| SKIP001101 | |
| SKIP ID | SKIP001101 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | o induced chondrogenic (iChon) cells |
| Cell Type(Ja) | ダイレクトリプログラミングで作製した軟骨細胞 |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Normal |
| Cell Name 1(En) | WT-1-iChon |
| Cell Name 1(Ja) | WT-1-iChon |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | 0-9 |
| Sex | Male |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | Direct iInduction of Chondrogenic(iChon) cells from Human Dermal Fibroblasts(1439 KURABO). |
| Description(Ja) | 新生児皮膚繊維芽細胞(1439 KURABO)をダイレクトリプログラミングにより軟骨細胞へと誘導（iChon細胞） |
| Cell Morphology | Other |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | c-MYC, KLF4,SOX9 |
| Adhesiveness | - |
| Feeder | No |
| Feeder Cell | - |
| Medium | DMEM (Sigma) with 10% FBS (Invitrogen), 50 U/ml penicillin and 50 μg/ml streptomycin. |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | a real-time RT-PCR expression analysis |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | Alcian blue and toluidine blue staining |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | - |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Minoru Okada |
| Author Name(Ja) | 岡田 稔 |
| Author Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| Author Organization(Ja) | 京都大学iPS細胞研究所 |
| Author Contact Email | ntsumaki[at]cira[dot]kyoto-u[dot]ac[dot]jp |
| PI Organization(En) | - |
| PI Organization(Ja) | - |
| PI Name(En) | - |
| PI Name(Ja) | - |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | Center for iPS Cell Research and Application,Kyoto University |
| Provider Organization(Ja) | 京都大学iPS細胞研究所 CiRA |
| Provider Email | - |
| Provider URL | https://www.cira.kyoto-u.ac.jp/e/index.html |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 25187577 |
| DOI | 10.1093/hmg/ddu444 |
| Title | Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells. |
| Authors | Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N |
| Journal | Hum Mol Genet |
| Year | 2014 |
| Volume | 24 |
| Issue | 2 |
| Pages | 299-313 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/25187577 |
| Free input | - |
| Note | - |