SKIP001101 |
SKIP ID |
SKIP001101 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
o induced chondrogenic (iChon) cells |
Cell Type(Ja) |
ダイレクトリプログラミングで作製した軟骨細胞 |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Normal |
Cell Name 1(En) |
WT-1-iChon |
Cell Name 1(Ja) |
WT-1-iChon |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
- |
ICD Code 1 |
- |
Disease Name 1(En) |
- |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
- |
Age Range |
0-9 |
Sex |
Male |
Race(En) |
- |
Race(Ja) |
- |
Genetic Diagnosis |
-- |
Not Detected |
No |
Description(En) |
Direct iInduction of Chondrogenic(iChon) cells from Human Dermal Fibroblasts(1439 KURABO). |
Description(Ja) |
新生児皮膚繊維芽細胞(1439 KURABO)をダイレクトリプログラミングにより軟骨細胞へと誘導(iChon細胞) |
Cell Morphology |
Other |
Grade |
Research Grade |
Vector |
Retrovirus |
Transgene |
c-MYC, KLF4,SOX9 |
Adhesiveness |
- |
Feeder |
No |
Feeder Cell |
- |
Medium |
DMEM (Sigma) with 10% FBS (Invitrogen), 50 U/ml penicillin and 50 μg/ml streptomycin. |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
Yes |
Pluripotent Markers Assay |
a real-time RT-PCR expression analysis |
in vitro Differentiation |
Yes |
in vitro Differentiation Assay |
Alcian blue and toluidine blue staining |
in vivo Differentiation |
- |
in vivo Differentiation Assay |
- |
Other 1 Assay |
- |
Other 1 Assay Method |
- |
Other 2 Assay |
- |
Other 2 Assay Method |
- |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
- |
Karyotype Assay |
- |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Minoru Okada |
Author Name(Ja) |
岡田 稔 |
Author Organization(En) |
Center for iPS Cell Research and Application, Kyoto University |
Author Organization(Ja) |
京都大学iPS細胞研究所 |
Author Contact Email |
ntsumaki[at]cira[dot]kyoto-u[dot]ac[dot]jp |
PI Organization(En) |
- |
PI Organization(Ja) |
- |
PI Name(En) |
- |
PI Name(Ja) |
- |
PI Contact Email |
- |
Availability |
Information Only |
Provider Organization(En) |
Center for iPS Cell Research and Application,Kyoto University |
Provider Organization(Ja) |
京都大学iPS細胞研究所 CiRA |
Provider Email |
- |
Provider URL |
https://www.cira.kyoto-u.ac.jp/e/index.html |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
25187577 |
DOI |
10.1093/hmg/ddu444 |
Title |
Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells. |
Authors |
Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N |
Journal |
Hum Mol Genet |
Year |
2014 |
Volume |
24 |
Issue |
2 |
Pages |
299-313 |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/25187577 |
Free input |
- |
Note |
- |