SKIP001103 | |
SKIP ID | SKIP001103 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | induced chondrogenic (iChon) cells |
Cell Type(Ja) | ダイレクトリプログラミングで作製した軟骨細胞 |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Diseased |
Cell Name 1(En) | ACGII-1-iChon |
Cell Name 1(Ja) | ACGII-1-iChon |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | 軟骨無発生症 |
ICD Code 1 | Q770 |
Disease Name 1(En) | Achondrogenesis type II (ACG2) |
OMIM1 | 200610 |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | - |
Age Range | Fetus |
Sex | Female |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | Yes |
Not Detected | No |
Description(En) | Direct iInduction of Chondrogenic(iChon) cells from ACGII-Human Dermal Fibroblasts(GM07892). COL2pathy-iChon cells have elevated ER stress and undergo apoptosis. |
Description(Ja) | 軟骨無発症患者線維芽細胞(GM07892)をダイレクトリプログラミングにより軟骨細胞へと誘導(iChon細胞)。 小胞体ストレスが生じ、過度のアポトーシスが引き起こされていた。 |
Cell Morphology | Other |
Grade | Research Grade |
Vector | Retrovirus |
Transgene | c-MYC, KLF4,SOX9 |
Adhesiveness | - |
Feeder | No |
Feeder Cell | - |
Medium | DMEM (Sigma) with 10% FBS (Invitrogen), 50 U/ml penicillin and 50 μg/ml streptomycin. |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | a real-time RT-PCR expression analysis |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | Alcian blue and toluidine blue staining |
in vivo Differentiation | - |
in vivo Differentiation Assay | - |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | - |
Karyotype Assay | - |
Remaining Vector Detection | - |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | Minoru Okada |
Author Name(Ja) | 岡田 稔 |
Author Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
Author Organization(Ja) | 京都大学iPS細胞研究所 |
Author Contact Email | ntsumaki[at]cira[dot]kyoto-u[dot]ac[dot]jp |
PI Organization(En) | - |
PI Organization(Ja) | - |
PI Name(En) | - |
PI Name(Ja) | - |
PI Contact Email | - |
Availability | Information Only |
Provider Organization(En) | Center for iPS Cell Research and Application,Kyoto University |
Provider Organization(Ja) | 京都大学iPS細胞研究所 CiRA |
Provider Email | - |
Provider URL | https://www.cira.kyoto-u.ac.jp/e/index.html |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 25187577 |
DOI | 10.1093/hmg/ddu444 |
Title | Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells. |
Authors | Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N |
Journal | Hum Mol Genet |
Year | 2014 |
Volume | 24 |
Issue | 2 |
Pages | 299-313 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/25187577 |
Free input | - |
Note | - |