| SKIP000858 | |
| SKIP ID | SKIP000858 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | αβT |
| Cell Tissue(Ja) | αβT |
| Cell Origin | Normal |
| Cell Name 1(En) | 585A1 |
| Cell Name 1(Ja) | 585A1 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 30 |
| Age Range | 30-39 |
| Sex | Male |
| Race(En) | Japanese |
| Race(Ja) | Japanese |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | hiPSC Generation from Peripheral Blood. Medium contained IL-2 and antibodies against CD3 and CD28 to stimulate the proliferation of T cells. |
| Description(Ja) | 末梢血由来iPS細胞。 T細胞の増殖を刺激する条件で作製を行った。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Plasmid |
| Transgene | Y4(OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA for TP53) |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | MEF |
| Medium | T medium,X-vivo10 (Lonza) supplemented with 30 U/ml IL-2 (PeproTech Inc., Rocky Hill, NJ, http://www.peprotech.com/) and 5 μl/well of Dynabeads Human T-activator CD3/CD28. |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | RT-PCR |
| in vitro Differentiation | - |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | Teratoma formation |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | Yes |
| Other 2 Assay Method | Microarray |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-band |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Keisuke Okita |
| Author Name(Ja) | 沖田 圭介 |
| Author Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| Author Organization(Ja) | 京都大学iPS細胞研究所 |
| Author Contact Email | - |
| PI Organization(En) | - |
| PI Organization(Ja) | - |
| PI Name(En) | - |
| PI Name(Ja) | - |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| Provider Organization(Ja) | 京都大学iPS細胞研究所 |
| Provider Email | - |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 23193063 -- 26198166 -- 26198166 |
| DOI | 10.1002/stem.1293 -- 10.5966/sctm.2014-0219 -- 10.5966/sctm.2014-0219 |
| Title | An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. -- Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice. -- Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice. |
| Authors | Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S -- Toyohara T, Mae S, Sueta S, Inoue T, Yamagishi Y, Kawamoto T, Kasahara T, Hoshina A, Toyoda T, Tanaka H, Araoka T, Sato-Otsubo A, Takahashi K, Sato Y, Yamaji N, Ogawa S, Yamanaka S, Osafune K -- Toyohara T, Mae S, Sueta S, Inoue T, Yamagishi Y, Kawamoto T, Kasahara T, Hoshina A, Toyoda T, Tanaka H, Araoka T, Sato-Otsubo A, Takahashi K, Sato Y, Yamaji N, Ogawa S, Yamanaka S, Osafune K |
| Journal | Stem Cells -- Stem Cells Transl Med -- Stem Cells Transl Med |
| Year | 2013 -- 2015 -- 2015 |
| Volume | 31 -- 4 -- 4 |
| Issue | 3 -- 9 -- 9 |
| Pages | 458-66 -- 980-92 -- 980-92 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/23193063 -- http://www.ncbi.nlm.nih.gov/pubmed/26198166 -- http://www.ncbi.nlm.nih.gov/pubmed/26198166 |
| Free input | -- -- |
| Note | Southern blot analysis The TRD locus is located within the TRA locus and is excised coincident with TRA rearrangement during the maturation of αβT cells. Hence, these data suggested that the four iPS clones were derived from αβT cells. αβT細胞由来であることを確認 |