| SKIP000581 | |
| SKIP ID | SKIP000581 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | HCM 090909 |
| Cell Name 1(Ja) | HCM 090909 |
| Cell Name 2(En) | HCM 1 |
| Cell Name 2(Ja) | HCM 1 |
| Disease Name 1(Ja) | 肥大型心筋症 |
| ICD Code 1 | I422 |
| Disease Name 1(En) | Hypertrophic cardiomyopathy |
| OMIM1 | 192600 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 63 |
| Age Range | 60-69 |
| Sex | Male |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | iPS cell lines from skin fibroblasts containing the TPM1-Arg91Cys mutation that might be causal mutation of hypertrophic cardiomyopathy, by using four classic retroviral vectors pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc encoding (mouse) reprogramming factors. Four classic retroviral vectors pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc encoding (mouse) reprogramming factors constructed by the laboratory of Dr Yamanaka were obtained from Addgene. |
| Description(Ja) | TPM1-Arg91Cys変異（肥大型心筋症の原因遺伝子の可能性をもつ）を持つ皮膚細胞からiPS細胞を樹立した。4つのレトロウイルスベクター（pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Mycを用いてiPS細胞を樹立。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | mouse embryonic fibroblast (MEF) |
| Medium | the standard hES cell medium (80% DMEM/F12, 20% KO Serum Replacement (Invitrogen) with 4 ng/ml basic fibroblast growth factor) |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | - |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | teratoma formation |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | No |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | - |
| Author Name(Ja) | - |
| Author Organization(En) | - |
| Author Organization(Ja) | - |
| Author Contact Email | - |
| PI Organization(En) | Department of Cardiology,Keio University School of Medicine |
| PI Organization(Ja) | 慶應義塾大学医学部循環器内科 |
| PI Name(En) | Shinsuke Yuasa |
| PI Name(Ja) | 湯浅 慎介 |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | - |
| Provider Organization(Ja) | - |
| Provider Email | - |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 25389285 |
| DOI | 10.1161/JAHA.114.001263 |
| Title | Endothelin-1 induces myofibrillar disarray and contractile vector variability in hypertrophic cardiomyopathy-induced pluripotent stem cell-derived cardiomyocytes. |
| Authors | Tanaka A, Yuasa S, Mearini G, Egashira T, Seki T, Kodaira M, Kusumoto D, Kuroda Y, Okata S, Suzuki T, Inohara T, Arimura T, Makino S, Kimura K, Kimura A, Furukawa T, Carrier L, Node K, Fukuda K |
| Journal | J Am Heart Assoc |
| Year | 2014 |
| Volume | 3 |
| Issue | 6 |
| Pages | - |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/25389285 |
| Free input | - |
| Note | - |