SKIP000585 | |
SKIP ID | SKIP000585 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | umbilical cord mononuclear blood cells |
Cell Tissue(Ja) | 臍帯血由来単核球 |
Cell Origin | Normal |
Cell Name 1(En) | UCBiPS7 |
Cell Name 1(Ja) | UCBiPS7 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | - |
ICD Code 1 | - |
Disease Name 1(En) | - |
OMIM1 | - |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | - |
Age Range | -- |
Sex | -- |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | -- |
Not Detected | No |
Description(En) | UCBiPS7 was reprogrammed from human umbilical cord mononuclear blood cells (from cord blood bank, IBC code number: 0802E26309) using Lentivirus (IBC code Number: 1109H04828) |
Description(Ja) | ヒト臍帯血単核細胞由来iPS細胞。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Lentivirus |
Transgene | OCT4, SOX2, KLF4, and C-MYC |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | MEF |
Medium | 85% DMEM/F12 supplemented with 15% knockout serum, 8 ng/mL bFGF, 0.56 Penicillin-Streptomycin, 16 Non-essential amino acid (NEAA), 1 mM glutamine, and 55 mM mercaptoeth- anol. |
Genome Editing | - |
CO2 | - |
Mycoplasma | Unknown |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | Immunohistochemistry Study |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | Cardiac Myocyte Differentiation |
in vivo Differentiation | No |
in vivo Differentiation Assay | - |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | No |
Karyotype Assay | - |
Remaining Vector Detection | - |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | Lei Ye |
Author Name(Ja) | Lei Ye |
Author Organization(En) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
Author Organization(Ja) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
Author Contact Email | zhang047[at]umn[dot]edu |
PI Organization(En) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
PI Organization(Ja) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
PI Name(En) | Jianyi Zhang |
PI Name(Ja) | Jianyi Zhang |
PI Contact Email | - |
Availability | Information Only |
Provider Organization(En) | - |
Provider Organization(Ja) | - |
Provider Email | - |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 23326500 -- 25479750 |
DOI | 10.1371/journal.pone.0053764 -- 10.1016/j.stem.2014.11.009 |
Title | Effective cardiac myocyte differentiation of human induced pluripotent stem cells requires VEGF. -- Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells. |
Authors | Ye L, Zhang S, Greder L, Dutton J, Keirstead SA, Lepley M, Zhang L, Kaufman D, Zhang J -- Ye L, Chang YH, Xiong Q, Zhang P, Zhang L, Somasundaram P, Lepley M, Swingen C, Su L, Wendel JS, Guo J, Jang A, Rosenbush D, Greder L, Dutton JR, Zhang J, Kamp TJ, Kaufman DS, Ge Y, Zhang J |
Journal | PLoS One -- Cell Stem Cell |
Year | 2013 -- 2014 |
Volume | 8 -- 15 |
Issue | 1 -- 6 |
Pages | e53764 -- 750-61 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/23326500 -- http://www.ncbi.nlm.nih.gov/pubmed/25479750 |
Free input | -- |
Note | iPS作成法は、PloS one 8, e53764、同一著者 |