SKIP000585
SKIP ID SKIP000585
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) umbilical cord mononuclear blood cells
Cell Tissue(Ja) 臍帯血由来単核球
Cell Origin Normal
Cell Name 1(En) UCBiPS7
Cell Name 1(Ja) UCBiPS7
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex --
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) UCBiPS7 was reprogrammed from human umbilical cord mononuclear blood cells (from cord blood bank, IBC code number: 0802E26309) using Lentivirus (IBC code Number: 1109H04828)
Description(Ja) ヒト臍帯血単核細胞由来iPS細胞。
Cell Morphology human ES-like
Grade Research Grade
Vector Lentivirus
Transgene OCT4, SOX2, KLF4, and C-MYC
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium 85% DMEM/F12 supplemented with 15% knockout serum, 8 ng/mL bFGF, 0.56 Penicillin-Streptomycin, 16 Non-essential amino acid (NEAA), 1 mM glutamine, and 55 mM mercaptoeth- anol.
Genome Editing -
CO2 -
Mycoplasma Unknown
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunohistochemistry Study
in vitro Differentiation Yes
in vitro Differentiation Assay Cardiac Myocyte Differentiation
in vivo Differentiation No
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype No
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Lei Ye
Author Name(Ja) Lei Ye
Author Organization(En) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
Author Organization(Ja) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
Author Contact Email zhang047[at]umn[dot]edu
PI Organization(En) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
PI Organization(Ja) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
PI Name(En) Jianyi Zhang
PI Name(Ja) Jianyi Zhang
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 23326500
--
25479750
DOI 10.1371/journal.pone.0053764
--
10.1016/j.stem.2014.11.009
Title Effective cardiac myocyte differentiation of human induced pluripotent stem cells requires VEGF.
--
Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.
Authors Ye L, Zhang S, Greder L, Dutton J, Keirstead SA, Lepley M, Zhang L, Kaufman D, Zhang J
--
Ye L, Chang YH, Xiong Q, Zhang P, Zhang L, Somasundaram P, Lepley M, Swingen C, Su L, Wendel JS, Guo J, Jang A, Rosenbush D, Greder L, Dutton JR, Zhang J, Kamp TJ, Kaufman DS, Ge Y, Zhang J
Journal PLoS One
--
Cell Stem Cell
Year 2013
--
2014
Volume 8
--
15
Issue 1
--
6
Pages e53764
--
750-61
URL http://www.ncbi.nlm.nih.gov/pubmed/23326500
--
http://www.ncbi.nlm.nih.gov/pubmed/25479750
Free input
--
Note iPS作成法は、PloS one 8, e53764、同一著者