| SKIP000587 | |
| SKIP ID | SKIP000587 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | human bone marrow CD34+ cells |
| Cell Tissue(Ja) | human bone marrow CD34+ cells |
| Cell Origin | Normal |
| Cell Name 1(En) | BC-1 |
| Cell Name 1(Ja) | BC-1 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | -- |
| Sex | Unknown |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | BC-1 was reprogrammed from human bone marrow CD34+ cells using non-integrate pEB-C5 vectors. |
| Description(Ja) | ヒトCD34+ 骨髄細胞由来iPS細胞。非組込型pEB-C5ベクターを用いて作製。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Plasmid |
| Transgene | OCT4, SOX2, KLF4, and C-MYC |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | MEF |
| Medium | DMEM/ F-12 supplemented with 15% Knockout Serum Replacer, 1 mM l -glutamine, 0.1 mM β-mercaptoethanol, 1% minimum essential medium nonessential amino acids, 4 ng/ml basic fibroblast growth factor. |
| Genome Editing | - |
| CO2 | 5 |
| Mycoplasma | Unknown |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | Immunohistochemistry Study |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | Differentiation |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | Yes |
| Other 1 Assay Method | Quantitative RT-PCR |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Lei Ye |
| Author Name(Ja) | Lei Ye |
| Author Organization(En) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
| Author Organization(Ja) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
| Author Contact Email | zhang047[at]umn[dot]edu |
| PI Organization(En) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
| PI Organization(Ja) | Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America |
| PI Name(En) | Jianyi Zhang |
| PI Name(Ja) | Jianyi Zhang |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | - |
| Provider Organization(Ja) | - |
| Provider Email | - |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 23326500 -- 25479750 |
| DOI | 10.1371/journal.pone.0053764 -- 10.1016/j.stem.2014.11.009 |
| Title | Effective cardiac myocyte differentiation of human induced pluripotent stem cells requires VEGF. -- Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells. |
| Authors | Ye L, Zhang S, Greder L, Dutton J, Keirstead SA, Lepley M, Zhang L, Kaufman D, Zhang J -- Ye L, Chang YH, Xiong Q, Zhang P, Zhang L, Somasundaram P, Lepley M, Swingen C, Su L, Wendel JS, Guo J, Jang A, Rosenbush D, Greder L, Dutton JR, Zhang J, Kamp TJ, Kaufman DS, Ge Y, Zhang J |
| Journal | PLoS One -- Cell Stem Cell |
| Year | 2013 -- 2014 |
| Volume | 8 -- 15 |
| Issue | 1 -- 6 |
| Pages | e53764 -- 750-61 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/23326500 -- http://www.ncbi.nlm.nih.gov/pubmed/25479750 |
| Free input | -- |
| Note | - |