| SKIP000616 | |
| SKIP ID | SKIP000616 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | LQTS2 |
| Cell Name 1(Ja) | LQTS2 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | QT延長症候群 type 2 |
| ICD Code 1 | I490 |
| Disease Name 1(En) | long QT syndrome 2 |
| OMIM1 | 613688 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 13 |
| Age Range | 10-19 |
| Sex | Male |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | Disease(LQTS2)-specific-induced pluripotent stem cells using the virus-free reprogramming method. Reprogramming of patient-derived skin fibroblasts were co-transfected with two oriP/EBNA1-based episomal plasmids (pEP4 EO2S CK2M EN2L, pEP4 EO2SET2K; Addgene Inc., Cambridge, MA, USA) via nucleofection (NHDF-VPD-1001 with U-20 program; Amaxa, Walkersville, MD, USA). |
| Description(Ja) | QT延長症候群 type2患者由来ウィルスフリーのiPS細胞。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Plasmid |
| Transgene | pEP4 EO2S CK2M EN2L, pEP4 EO2SET2K |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | MEFs |
| Medium | DMEM/F12 culture medium supplemented with 20% KnockOut serum replacer, 0.1 mM non-essential amino acids (all from Invitrogen, Carlsbad, CA), 1 mM L-glutamine, 0.1 mM β-mercaptoethanol and 100 ng/ml zebrafish basic fibroblast growth factor (zbFGF) |
| Genome Editing | - |
| CO2 | 5% |
| Mycoplasma | Unknown |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | Immunofluorscence |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | EB formation and cardiomyocyte differentiation |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | Teratoma formation |
| Other 1 Assay | Yes |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-banding chromosome analysis |
| Remaining Vector Detection | Yes |
| Remaining Vector Detection Assay | Genomic PCR |
| STR | Yes |
| HLA | - |
| Stem Cell Transcriptome analysis | Yes |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Winston Shim |
| Author Name(Ja) | Winston Shim |
| Author Organization(En) | Research and Development Unit, National Heart Centre Singapore |
| Author Organization(Ja) | Research and Development Unit, National Heart Centre Singapore |
| Author Contact Email | winston[dot]shim[dot]s[dot]n[at]nhcs[dot]com[dot]sg |
| PI Organization(En) | Research and Development Unit, National Heart Centre Singapore |
| PI Organization(Ja) | Research and Development Unit, National Heart Centre Singapore |
| PI Name(En) | Winston Shim |
| PI Name(Ja) | Winston Shim |
| PI Contact Email | winston[dot]shim[dot]s[dot]n[at]nhcs[dot]com[dot]sg |
| Availability | Information Only |
| Provider Organization(En) | - |
| Provider Organization(Ja) | - |
| Provider Email | - |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 24623279 -- 21565833 -- 19325077 |
| DOI | 10.1093/cvr/cvu060 -- 10.1093/cvr/cvr132 -- 10.1126/science.1172482 |
| Title | Re-trafficking of hERG reverses long QT syndrome 2 phenotype in human iPS-derived cardiomyocytes. -- Pharmacological response of human cardiomyocytes derived from virus-free induced pluripotent stem cells. -- Human induced pluripotent stem cells free of vector and transgene sequences. |
| Authors | Mehta A, Sequiera GL, Ramachandra CJ, Sudibyo Y, Chung Y, Sheng J, Wong KY, Tan TH, Wong P, Liew R, Shim W -- Mehta A, Chung YY, Ng A, Iskandar F, Atan S, Wei H, Dusting G, Sun W, Wong P, Shim W -- Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA |
| Journal | Cardiovasc Res -- Cardiovasc Res -- Science |
| Year | 2014 -- 2011 -- 2009 |
| Volume | 102 -- 91 -- 324 |
| Issue | 3 -- 4 -- 5928 |
| Pages | 497-506 -- 577-86 -- 797-801 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/24623279 -- http://www.ncbi.nlm.nih.gov/pubmed/21565833 -- http://www.ncbi.nlm.nih.gov/pubmed/19325077 |
| Free input | -- -- |
| Note | - |