SKIP000588
SKIP ID SKIP000588
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) GRiPS
Cell Name 1(Ja) GRiPS
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range 0-9
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) GRiPS16 was reprogrammed from neonatal human dermal fibroblasts by GFP-expressing Sendai virus vector.
Description(Ja) ヒト新生児皮膚線維芽細胞由来iPS細胞。GFP発現センダイウイルスベクターを用いて作製。
Cell Morphology human ES-like
Grade Research Grade
Vector Sendai virus
Transgene OCT4, SOX2, KLF4, and C-MYC
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium DMEM/ F-12 supplemented with 15% Knockout Serum Replacer, 1 mM l -glutamine, 0.1 mM β-mercaptoethanol, 1% minimum essential medium nonessential amino acids, 4 ng/ml basic fibroblast growth factor.
Genome Editing -
CO2 5
Mycoplasma Unknown
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunohistochemistry Study
in vitro Differentiation Yes
in vitro Differentiation Assay Differentiation
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay Yes
Other 1 Assay Method Quantitative RT-PCR
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Lei Ye
Author Name(Ja) Lei Ye
Author Organization(En) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
Author Organization(Ja) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
Author Contact Email zhang047[at]umn[dot]edu
PI Organization(En) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
PI Organization(Ja) Division of Cardiology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
PI Name(En) Jianyi Zhang
PI Name(Ja) Jianyi Zhang
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 23326500
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25479750
DOI 10.1371/journal.pone.0053764
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10.1016/j.stem.2014.11.009
Title Effective cardiac myocyte differentiation of human induced pluripotent stem cells requires VEGF.
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Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.
Authors Ye L, Zhang S, Greder L, Dutton J, Keirstead SA, Lepley M, Zhang L, Kaufman D, Zhang J
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Ye L, Chang YH, Xiong Q, Zhang P, Zhang L, Somasundaram P, Lepley M, Swingen C, Su L, Wendel JS, Guo J, Jang A, Rosenbush D, Greder L, Dutton JR, Zhang J, Kamp TJ, Kaufman DS, Ge Y, Zhang J
Journal PLoS One
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Cell Stem Cell
Year 2013
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2014
Volume 8
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15
Issue 1
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6
Pages e53764
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750-61
URL http://www.ncbi.nlm.nih.gov/pubmed/23326500
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http://www.ncbi.nlm.nih.gov/pubmed/25479750
Free input
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