SKIP001110 | |
SKIP ID | SKIP001110 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Normal |
Cell Name 1(En) | C1-2 |
Cell Name 1(Ja) | C1-2 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | - |
ICD Code 1 | - |
Disease Name 1(En) | - |
OMIM1 | - |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | - |
Age Range | 0-9 |
Sex | Male |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | -- |
Not Detected | No |
Description(En) | iPS cells from fibroblasts a control individual outside of the pedigree as an additional control (ATCC(CRL-2097)) |
Description(Ja) | 血縁関係のない正常な個人由来のiPS細胞(コントロール) |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Plasmid |
Transgene | OCT4,SOX2,KLF4,c-MYC,LIN28,SV40LT. pEP4 EO2S ET2K (Addgene Plasmid 20927), pEP4 EO2S EN2L (Addgene Plasmid 20922), and pEP4 EO2S EM2K (Addgene Plasmid 20923) |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | MEF |
Medium | D-MEM/F12 (Invitrogen), 20% Knockout Serum Replacement (KSR, Invitrogen), 2 mM l-glutamine (Invitrogen), 100 uM MEM NEAA (Invitrogen), 100 uM β-mercaptoethanol (Invitrogen), and 10 ng ml-1 human basic FGF (bFGF, PeproTech) |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | Immunostaining |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | Embryoid body assay |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | Teratoma formation |
Other 1 Assay | Yes |
Other 1 Assay Method | Differentiation of iPS cells into forebrain-specific neural progenitors and cortical neurons |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | G-banding |
Remaining Vector Detection | - |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | - |
Author Name(Ja) | - |
Author Organization(En) | - |
Author Organization(Ja) | - |
Author Contact Email | - |
PI Organization(En) | Johns Hopkins University School of Medicine |
PI Organization(Ja) | ジョンスホプキンス大学 |
PI Name(En) | Hongjun Song |
PI Name(Ja) | 宋红军 |
PI Contact Email | - |
Availability | Information Only |
Provider Organization(En) | - |
Provider Organization(Ja) | - |
Provider Email | - |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 25132547 |
DOI | 10.1038/nature13716 |
Title | Synaptic dysregulation in a human iPS cell model of mental disorders. |
Authors | Wen Z, Nguyen HN, Guo Z, Lalli MA, Wang X, Su Y, Kim NS, Yoon KJ, Shin J, Zhang C, Makri G, Nauen D, Yu H, Guzman E, Chiang CH, Yoritomo N, Kaibuchi K, Zou J, Christian KM, Cheng L, Ross CA, Margolis RL, Chen G, Kosik KS, Song H, Ming GL |
Journal | Nature |
Year | 2014 |
Volume | 515 |
Issue | 7527 |
Pages | 414-8 |
URL | - |
Free input | - |
Note | - |