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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
BP911459
Clone id YMU001_000005_C01
Library
Length 607
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000005_C01.
Accession
Tissue type prothallium
Developmental stage -
Contig ID -
Sequence 
CAGAGCATGATGACAGGAGGCTGGATCTTGTGCTTGTCCACAAAGTGGGCCGACTTCAAT
TGCTTAGATTTTTGGTCCTAATGCAATTTGGCAGACACCTTTCGTTACCGTTCGTAGAGT
ACACAAAGGTGCAATCGGTGAAACTCAAGCCTGGGAATAATCACCATAAAGGTTGTGGAA
TAGATGGAGAACTCATGCCTCTCAATGGACCCATCTCAACCACTATTCTTGAGGAGCAAT
GCCAACTCATTGGGCACTCTGTTTTCAGGCATTAAGGTGGAGTAAGGAAGGCAGTTTTAC
TGTACAGGTATAGGAAGCTTTTCTTACAAGGGTGTTCCAACACAAGCTGCTTTTGGAGAC
CTTAATTTTGCATCAAGGTCTTTGTATCAATATTTGTAGATTCTAGCTTTTATGTCATTT
ACTTTCACAAAGTGGCCTAAAATTGTTGAGCGGAGATTATGGTTGTCAGAACATGGGATC
CTCTTTTCAAATAAAGCCACCAGGACTCAATCTACACTGATTTCCATGTACATCATGACC
ATGTGGGCGTGAGTGATTATTTATTCTTTTTGTAAAATAAGTTCAATGGTGGGACCCATC
GCTCTTA
■■Homology search results ■■ -
sp_hit_id Q9LRB0
Definition sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Arabidopsis thaliana
Align length 90
Score (bit) 116.0
E-value 8.0e-26
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP911459|Adiantum capillus-veneris mRNA, clone:
YMU001_000005_C01.
         (607 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Ara...   116   8e-26
sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium disc...    37   0.10 
sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium disc...    35   0.30 
sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SP...    34   0.66 
sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sp...    33   1.5  
sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sp...    31   4.3  

>sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1
           OS=Arabidopsis thaliana GN=LCBK1 PE=1 SV=1
          Length = 763

 Score =  116 bits (291), Expect = 8e-26
 Identities = 54/90 (60%), Positives = 70/90 (77%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           EHDD  +D++LVH  GRL+LLRF +L+Q GRHLSLP+VE  KV+SVK+K G N H  CGI
Sbjct: 673 EHDDGTMDMLLVHGCGRLRLLRFFILLQTGRHLSLPYVECVKVKSVKIKAGKNTHDSCGI 732

Query: 183 DGELMPLNGPISTTILEEQCQLIGHSVFRH 272
           DGEL  L+G + +T+L EQC+LIG++  RH
Sbjct: 733 DGELFALHGEVISTMLPEQCRLIGNAPGRH 762


>sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium
           discoideum GN=sgkB PE=2 SV=1
          Length = 760

 Score = 36.6 bits (83), Expect = 0.10
 Identities = 21/66 (31%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
 Frame = +3

Query: 12  DRRLDLVLVH---KVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           D  +DL+ ++   K+ +  LL  L     G HL    +E+ KV+++ L+P    H    I
Sbjct: 674 DGLIDLIFINNRSKLSKASLLSILTDSATGDHLKSDLIEHHKVKALILEPSIQKHGIVAI 733

Query: 183 DGELMP 200
           DGE +P
Sbjct: 734 DGERIP 739


>sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium
           discoideum GN=sgkA PE=2 SV=2
          Length = 624

 Score = 35.0 bits (79), Expect = 0.30
 Identities = 20/65 (30%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
 Frame = +3

Query: 12  DRRLDLVLVH---KVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           D  +DLV+++   K  +  LL  L     G H+    ++  KVQ++ L+P N+      +
Sbjct: 539 DGLIDLVVINNNKKFSKAGLLSVLTESSTGAHVKSDLIDQYKVQAMILEPSNDREGIIAV 598

Query: 183 DGELM 197
           DGEL+
Sbjct: 599 DGELI 603


>sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1
           PE=1 SV=1
          Length = 384

 Score = 33.9 bits (76), Expect = 0.66
 Identities = 21/54 (38%), Positives = 31/54 (57%), Gaps = 3/54 (5%)
 Frame = +3

Query: 45  VGRLQLLRFLVLMQFGRHL--SLPFVEYTKVQSVKLKPGNNHHKGC-GIDGELM 197
           V R  LLR  + M+ GRH+    P++ Y  V + +L+P +   KG   +DGELM
Sbjct: 294 VSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG--KGVFAVDGELM 345


>sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sphk1
           PE=1 SV=1
          Length = 382

 Score = 32.7 bits (73), Expect = 1.5
 Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
 Frame = +3

Query: 45  VGRLQLLRFLVLMQFGRHLSL--PFVEYTKVQSVKLKPGNNHHKGCGIDGELM 197
           V R  LLR  + MQ G+H+ L  P++ +  V + +L+P  +      +DGELM
Sbjct: 293 VSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEP-RSQRGVFSVDGELM 344


>sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sphk2
           PE=1 SV=2
          Length = 617

 Score = 31.2 bits (69), Expect = 4.3
 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 2/63 (3%)
 Frame = +3

Query: 45  VGRLQLLRFLVLMQFGRHLSL--PFVEYTKVQSVKLKPGNNHHKGCGIDGELMPLNGPIS 218
           + R  LLR L+ M+ G H SL  P + Y   ++ +L+P         +DGEL+   GPI 
Sbjct: 539 ISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGL-LTVDGELVEY-GPIQ 596

Query: 219 TTI 227
             +
Sbjct: 597 AQV 599
tr_hit_id A7R6E5
Definition tr|A7R6E5|A7R6E5_VITVI Chromosome undetermined scaffold_1258, whole genome shotgun sequence OS=Vitis vinifera
Align length 90
Score (bit) 127.0
E-value 5.0e-28
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP911459|Adiantum capillus-veneris mRNA, clone:
YMU001_000005_C01.
         (607 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A7R6E5|A7R6E5_VITVI Chromosome undetermined scaffold_1258, wh...   127   5e-28
tr|A7R3V9|A7R3V9_VITVI Chromosome undetermined scaffold_574, who...   127   5e-28
tr|A5AZR8|A5AZR8_VITVI Putative uncharacterized protein OS=Vitis...   127   5e-28
tr|Q5KR50|Q5KR50_LOTJA Sphingosine kinase OS=Lotus japonicus GN=...   122   2e-26
tr|B4FBT9|B4FBT9_MAIZE Putative uncharacterized protein OS=Zea m...   114   3e-24
tr|A9SVP5|A9SVP5_PHYPA Predicted protein OS=Physcomitrella paten...   114   3e-24
tr|Q7XN57|Q7XN57_ORYSJ Os04g0541500 protein OS=Oryza sativa subs...   114   6e-24
tr|Q01IK1|Q01IK1_ORYSA H0501D11.3 protein OS=Oryza sativa GN=H05...   114   6e-24
tr|B8ASL7|B8ASL7_ORYSI Putative uncharacterized protein OS=Oryza...   114   6e-24
tr|A9THB6|A9THB6_PHYPA Predicted protein (Fragment) OS=Physcomit...   110   6e-23
tr|Q94HY9|Q94HY9_ORYSA Putative sphingosine kinase OS=Oryza sati...   107   4e-22
tr|Q7XCS9|Q7XCS9_ORYSJ Os10g0516800 protein OS=Oryza sativa subs...   107   4e-22
tr|A3C6H6|A3C6H6_ORYSJ Putative uncharacterized protein OS=Oryza...   107   4e-22
tr|A2Z9C6|A2Z9C6_ORYSI Putative uncharacterized protein OS=Oryza...   107   4e-22
tr|B8BAQ3|B8BAQ3_ORYSI Putative uncharacterized protein OS=Oryza...    79   2e-13
tr|Q84S01|Q84S01_ORYSJ Os08g0152700 protein OS=Oryza sativa subs...    79   3e-13
tr|Q5K5B4|Q5K5B4_ORYSA Putative sphingosine kinase protein (Frag...    79   3e-13
tr|B3H501|B3H501_ARATH Uncharacterized protein At5g23450.3 OS=Ar...    61   6e-08
tr|A3AW08|A3AW08_ORYSJ Putative uncharacterized protein OS=Oryza...    60   8e-08
tr|A2YR99|A2YR99_ORYSI Putative uncharacterized protein OS=Oryza...    59   3e-07
tr|A6RQL8|A6RQL8_BOTFB Putative uncharacterized protein OS=Botry...    46   0.001
tr|Q1IM15|Q1IM15_ACIBL Sphingosine kinase related enzyme OS=Acid...    42   0.028
tr|B1HXA2|B1HXA2_LYSSC Putative uncharacterized protein OS=Lysin...    42   0.028
tr|Q4Q9C3|Q4Q9C3_LEIMA Putative uncharacterized protein OS=Leish...    42   0.036
tr|A4I203|A4I203_LEIIN Putative uncharacterized protein OS=Leish...    42   0.036
tr|A7EX08|A7EX08_SCLS1 Putative uncharacterized protein OS=Scler...    42   0.036
tr|A9TQY5|A9TQY5_PHYPA Predicted protein (Fragment) OS=Physcomit...    41   0.047
tr|A4HES6|A4HES6_LEIBR Putative uncharacterized protein OS=Leish...    41   0.047
tr|Q00YM9|Q00YM9_OSTTA Putative sphingosine kinase (ISS) OS=Ostr...    40   0.081
tr|Q57WS6|Q57WS6_9TRYP Putative uncharacterized protein OS=Trypa...    40   0.11 

>tr|A7R6E5|A7R6E5_VITVI Chromosome undetermined scaffold_1258, whole
           genome shotgun sequence OS=Vitis vinifera
           GN=GSVIVT00008144001 PE=4 SV=1
          Length = 463

 Score =  127 bits (319), Expect = 5e-28
 Identities = 58/90 (64%), Positives = 73/90 (81%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           E+DD  LDL+LVH  GRL+LLRF +L+QFGRHLSLP+VEY KV+SVK+KPG + H GCGI
Sbjct: 373 EYDDNTLDLLLVHGSGRLRLLRFFLLLQFGRHLSLPYVEYVKVKSVKIKPGKHTHNGCGI 432

Query: 183 DGELMPLNGPISTTILEEQCQLIGHSVFRH 272
           DGEL+P NG + +++L EQC+LIG    RH
Sbjct: 433 DGELLPTNGQVISSLLPEQCRLIGRPPNRH 462


>tr|A7R3V9|A7R3V9_VITVI Chromosome undetermined scaffold_574, whole
           genome shotgun sequence (Fragment) OS=Vitis vinifera
           GN=GSVIVT00003981001 PE=4 SV=1
          Length = 123

 Score =  127 bits (319), Expect = 5e-28
 Identities = 58/90 (64%), Positives = 73/90 (81%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           E+DD  LDL+LVH  GRL+LLRF +L+QFGRHLSLP+VEY KV+SVK+KPG + H GCGI
Sbjct: 33  EYDDNTLDLLLVHGSGRLRLLRFFLLLQFGRHLSLPYVEYVKVKSVKIKPGKHTHNGCGI 92

Query: 183 DGELMPLNGPISTTILEEQCQLIGHSVFRH 272
           DGEL+P NG + +++L EQC+LIG    RH
Sbjct: 93  DGELLPTNGQVISSLLPEQCRLIGRPPNRH 122


>tr|A5AZR8|A5AZR8_VITVI Putative uncharacterized protein OS=Vitis
            vinifera GN=VITISV_026658 PE=4 SV=1
          Length = 1062

 Score =  127 bits (319), Expect = 5e-28
 Identities = 58/90 (64%), Positives = 73/90 (81%)
 Frame = +3

Query: 3    EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
            E+DD  LDL+LVH  GRL+LLRF +L+QFGRHLSLP+VEY KV+SVK+KPG + H GCGI
Sbjct: 972  EYDDNTLDLLLVHGSGRLRLLRFFLLLQFGRHLSLPYVEYVKVKSVKIKPGKHTHNGCGI 1031

Query: 183  DGELMPLNGPISTTILEEQCQLIGHSVFRH 272
            DGEL+P NG + +++L EQC+LIG    RH
Sbjct: 1032 DGELLPTNGQVISSLLPEQCRLIGRPPNRH 1061


>tr|Q5KR50|Q5KR50_LOTJA Sphingosine kinase OS=Lotus japonicus
           GN=LjLCBK1 PE=2 SV=1
          Length = 788

 Score =  122 bits (305), Expect = 2e-26
 Identities = 56/84 (66%), Positives = 69/84 (82%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           EHDD  LDLVLVH  GRL+L+RF VL+Q GRHLSLP+VE  KV+SV++KPG + H GCGI
Sbjct: 701 EHDDNTLDLVLVHGNGRLKLIRFFVLLQMGRHLSLPYVENIKVKSVRIKPGKHTHNGCGI 760

Query: 183 DGELMPLNGPISTTILEEQCQLIG 254
           DGEL PLNG + +++L EQC+LIG
Sbjct: 761 DGELFPLNGQVISSLLPEQCRLIG 784


>tr|B4FBT9|B4FBT9_MAIZE Putative uncharacterized protein OS=Zea mays
           PE=2 SV=1
          Length = 304

 Score =  114 bits (286), Expect = 3e-24
 Identities = 54/83 (65%), Positives = 65/83 (78%)
 Frame = +3

Query: 6   HDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGID 185
           HDD  LDL+LV   GR +LLRF +L+QFGRHLSLP+VEY KV+SVKLKPG N H GCGID
Sbjct: 215 HDDNSLDLLLVGGKGRWKLLRFFILLQFGRHLSLPYVEYVKVKSVKLKPGANTHNGCGID 274

Query: 186 GELMPLNGPISTTILEEQCQLIG 254
           GEL  + G +  ++L EQC+LIG
Sbjct: 275 GELCRVKGQVLCSLLPEQCRLIG 297


>tr|A9SVP5|A9SVP5_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_84602 PE=4 SV=1
          Length = 646

 Score =  114 bits (286), Expect = 3e-24
 Identities = 55/84 (65%), Positives = 66/84 (78%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           EHDD  + L+LV  VGRLQL+RF +LMQFGRHLSLPFVEYTK ++V LKP    H+GCGI
Sbjct: 558 EHDDGLVHLLLVRDVGRLQLIRFFLLMQFGRHLSLPFVEYTKARAVWLKPAPRAHQGCGI 617

Query: 183 DGELMPLNGPISTTILEEQCQLIG 254
           DGEL+ L+GPI+T +   QC LIG
Sbjct: 618 DGELLTLDGPITTRMQPFQCHLIG 641


>tr|Q7XN57|Q7XN57_ORYSJ Os04g0541500 protein OS=Oryza sativa subsp.
           japonica GN=OSJNBb0103I08.5 PE=2 SV=2
          Length = 748

 Score =  114 bits (284), Expect = 6e-24
 Identities = 53/83 (63%), Positives = 65/83 (78%)
 Frame = +3

Query: 6   HDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGID 185
           HDD  LDL+LV   GR +LL+F +L+QFGRHLSLP+VEY KV+SVKLKPG N H GCGID
Sbjct: 659 HDDNTLDLLLVGGKGRWKLLKFFILLQFGRHLSLPYVEYVKVKSVKLKPGANTHNGCGID 718

Query: 186 GELMPLNGPISTTILEEQCQLIG 254
           GEL  + G +  ++L+EQC LIG
Sbjct: 719 GELCRVKGHVLCSLLQEQCMLIG 741


>tr|Q01IK1|Q01IK1_ORYSA H0501D11.3 protein OS=Oryza sativa
           GN=H0501D11.3 PE=4 SV=1
          Length = 748

 Score =  114 bits (284), Expect = 6e-24
 Identities = 53/83 (63%), Positives = 65/83 (78%)
 Frame = +3

Query: 6   HDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGID 185
           HDD  LDL+LV   GR +LL+F +L+QFGRHLSLP+VEY KV+SVKLKPG N H GCGID
Sbjct: 659 HDDNTLDLLLVGGKGRWKLLKFFILLQFGRHLSLPYVEYVKVKSVKLKPGANTHNGCGID 718

Query: 186 GELMPLNGPISTTILEEQCQLIG 254
           GEL  + G +  ++L+EQC LIG
Sbjct: 719 GELCRVKGHVLCSLLQEQCMLIG 741


>tr|B8ASL7|B8ASL7_ORYSI Putative uncharacterized protein OS=Oryza
           sativa subsp. indica GN=OsI_16825 PE=4 SV=1
          Length = 324

 Score =  114 bits (284), Expect = 6e-24
 Identities = 53/83 (63%), Positives = 65/83 (78%)
 Frame = +3

Query: 6   HDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGID 185
           HDD  LDL+LV   GR +LL+F +L+QFGRHLSLP+VEY KV+SVKLKPG N H GCGID
Sbjct: 235 HDDNTLDLLLVGGKGRWKLLKFFILLQFGRHLSLPYVEYVKVKSVKLKPGANTHNGCGID 294

Query: 186 GELMPLNGPISTTILEEQCQLIG 254
           GEL  + G +  ++L+EQC LIG
Sbjct: 295 GELCRVKGHVLCSLLQEQCMLIG 317


>tr|A9THB6|A9THB6_PHYPA Predicted protein (Fragment)
           OS=Physcomitrella patens subsp. patens
           GN=PHYPADRAFT_222331 PE=4 SV=1
          Length = 593

 Score =  110 bits (275), Expect = 6e-23
 Identities = 52/84 (61%), Positives = 64/84 (76%)
 Frame = +3

Query: 3   EHDDRRLDLVLVHKVGRLQLLRFLVLMQFGRHLSLPFVEYTKVQSVKLKPGNNHHKGCGI 182
           EHDD  + L+LV  VGRLQL+RF +LMQFGRHLSLPFVEY+K ++V LKP    H  CG+
Sbjct: 510 EHDDGVVHLLLVRNVGRLQLMRFFLLMQFGRHLSLPFVEYSKARAVWLKPAPGAHHCCGV 569

Query: 183 DGELMPLNGPISTTILEEQCQLIG 254
           DGEL+ L+GPI+T +    CQLIG
Sbjct: 570 DGELLTLDGPITTRVQPHSCQLIG 593