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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
BP913034
Clone id YMU001_000025_F09
Library
Length 548
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000025_F09.
Accession
Tissue type prothallium
Developmental stage -
Contig ID
Sequence 
GGAAAACGCAGCCTTAGTGTTGTGACCACGACAAAGTCGGCCTCAAGGAGCATGCAGACC
AGCAGACAAAGTCAGCTTCGAGCAGCATGCAGACCAGTAGACTTTCGTGTTTCCAGTGTT
TCCGTCTTGGGAGCCTCACACAAAGAGATTTTGAGGCCAAGGTCTAGTGCTGAAGGGAAC
ATACGCCTTCTGACCATCCATGGAGATATTTCAAATAAACTGAACGATGGCTGGGAGTCC
ATAGCATTGGAATATTGCATCAAATGGCCTCTGCAGCTACTTTTCACTAAAGAAACTTTG
TCAAAGTAAGGGCTTGCACCTTTATTGAATCGTGTCAAGCATCTCTTGCAGTGATGTTGA
AGCTTTTTGATTTCAGAAATCTCCACTAGGTGGCAATCCATAGATGCAGGAAGAGTTGGA
AGTGTTGAGGTTTTCTTTATGGCACATGTGTGTAAGGTGTCGTAAATTTTAGTCGGAGCC
TTCAGAGTATGTTGACCGTGAATGTTATTGCAAAGACATGCTTTGGTTAGCTATACATGC
CTTCATTT
■■Homology search results ■■ -
sp_hit_id Q9M350
Definition sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog OS=Arabidopsis thaliana
Align length 71
Score (bit) 49.7
E-value 9.0e-06
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP913034|Adiantum capillus-veneris mRNA, clone:
YMU001_000025_F09.
         (548 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog O...    50   9e-06
sp|Q9SC88|GCP4_MEDTR Gamma-tubulin complex component 4 homolog O...    47   8e-05
sp|Q9D4F8|GCP4_MOUSE Gamma-tubulin complex component 4 OS=Mus mu...    35   0.31 
sp|Q9UGJ1|GCP4_HUMAN Gamma-tubulin complex component 4 OS=Homo s...    34   0.41 
sp|P40114|TOP1_BACAN DNA topoisomerase 1 OS=Bacillus anthracis G...    32   1.5  
sp|Q06679|UTP4_YEAST U3 small nucleolar RNA-associated protein 4...    32   2.0  
sp|Q5R5J6|GCP2_PONAB Gamma-tubulin complex component 2 OS=Pongo ...    32   2.0  
sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 OS=Mus mu...    32   2.0  
sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 OS=Homo s...    32   2.0  
sp|P13267|DPO3_BACSU DNA polymerase III polC-type OS=Bacillus su...    31   3.4  
sp|Q8NEE8|TTC16_HUMAN Tetratricopeptide repeat protein 16 OS=Hom...    31   4.5  
sp|P34477|UBC7_CAEEL Probable ubiquitin-conjugating enzyme E2 7 ...    30   5.9  
sp|Q6QD51|CCD80_RAT Coiled-coil domain-containing protein 80 OS=...    30   5.9  
sp|Q8R2G6|CCD80_MOUSE Coiled-coil domain-containing protein 80 O...    30   5.9  
sp|Q5UQ61|YR661_MIMIV Uncharacterized protein R661 OS=Acanthamoe...    30   7.7  

>sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog
           OS=Arabidopsis thaliana GN=At3g53760 PE=2 SV=2
          Length = 745

 Score = 49.7 bits (117), Expect = 9e-06
 Identities = 28/71 (39%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
 Frame = +1

Query: 97  VDFRVSSVSV-LGASHKEILRPRSSAEGNIRLLTIHGDISNKLNDGWESIALEYCIKWPL 273
           V  R+ S  V + +S  +++R + S  G   L       S+   DGW++IALEY + WP+
Sbjct: 466 VSLRMPSFGVTVRSSQADMVRSKVSLTGKANLT------SDTSVDGWDAIALEYSVDWPM 519

Query: 274 QLLFTKETLSK 306
           QL FT+E LSK
Sbjct: 520 QLFFTQEVLSK 530


>sp|Q9SC88|GCP4_MEDTR Gamma-tubulin complex component 4 homolog
           OS=Medicago truncatula GN=85P PE=2 SV=1
          Length = 739

 Score = 46.6 bits (109), Expect = 8e-05
 Identities = 23/48 (47%), Positives = 29/48 (60%)
 Frame = +1

Query: 163 SSAEGNIRLLTIHGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 306
           S+A   I   +I    S    DGW+ IALEY I+WPL L FT+E LS+
Sbjct: 472 SAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSR 519


>sp|Q9D4F8|GCP4_MOUSE Gamma-tubulin complex component 4 OS=Mus
           musculus GN=Tubgcp4 PE=2 SV=2
          Length = 667

 Score = 34.7 bits (78), Expect = 0.31
 Identities = 12/28 (42%), Positives = 18/28 (64%)
 Frame = +1

Query: 223 NDGWESIALEYCIKWPLQLLFTKETLSK 306
           + GW ++ L Y ++WPL +LFT   L K
Sbjct: 447 SSGWAALGLSYKVQWPLHILFTPAVLEK 474


>sp|Q9UGJ1|GCP4_HUMAN Gamma-tubulin complex component 4 OS=Homo
           sapiens GN=TUBGCP4 PE=1 SV=1
          Length = 667

 Score = 34.3 bits (77), Expect = 0.41
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +1

Query: 229 GWESIALEYCIKWPLQLLFTKETLSK 306
           GW ++ L Y ++WPL +LFT   L K
Sbjct: 449 GWAALGLSYKVQWPLHILFTPAVLEK 474


>sp|P40114|TOP1_BACAN DNA topoisomerase 1 OS=Bacillus anthracis
           GN=topX PE=3 SV=2
          Length = 870

 Score = 32.3 bits (72), Expect = 1.5
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = -1

Query: 308 YFDKVSLVKSSCRGHLMQYSNAMDSQPSFSLFEI 207
           Y++    + S CRGHL++  N  +  P + LF++
Sbjct: 21  YYENDHYIVSWCRGHLLELKNPEEMDPKYKLFQL 54


>sp|Q06679|UTP4_YEAST U3 small nucleolar RNA-associated protein 4
           OS=Saccharomyces cerevisiae GN=UTP4 PE=1 SV=1
          Length = 776

 Score = 32.0 bits (71), Expect = 2.0
 Identities = 22/91 (24%), Positives = 47/91 (51%), Gaps = 10/91 (10%)
 Frame = +1

Query: 10  SLSVVTTTKSASRSMQ--------TSRQSQLRAACRPVDFRVSSVSVLGASHKEILRPRS 165
           S S+++  K  SRS++        TS++  +   CR VDF  ++++ L  SHK  +   +
Sbjct: 2   SSSLLSVLKEKSRSLKIRNKPVKMTSQERMIVHRCRFVDFTPATITSLAFSHKSNINKLT 61

Query: 166 SAEGNIRLLTIHGDIS--NKLNDGWESIALE 252
            ++  + +   +G+I   N  N+ ++ + +E
Sbjct: 62  PSDLRLAIGRSNGNIEIWNPRNNWFQEMVIE 92


>sp|Q5R5J6|GCP2_PONAB Gamma-tubulin complex component 2 OS=Pongo
           abelii GN=TUBGCP2 PE=2 SV=1
          Length = 902

 Score = 32.0 bits (71), Expect = 2.0
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +1

Query: 199 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 306
           H D +     G E+ + +Y +KWPL L+  ++ L++
Sbjct: 602 HADPTELTLSGLEAFSFDYIVKWPLSLIINRKALTR 637


>sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 OS=Mus
           musculus GN=Tubgcp2 PE=2 SV=1
          Length = 905

 Score = 32.0 bits (71), Expect = 2.0
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +1

Query: 199 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 306
           H D +     G E+ + +Y +KWPL L+  ++ L++
Sbjct: 602 HADPTELTLSGLEAFSFDYMVKWPLSLIINRKALTR 637


>sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 OS=Homo
           sapiens GN=TUBGCP2 PE=1 SV=2
          Length = 902

 Score = 32.0 bits (71), Expect = 2.0
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +1

Query: 199 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 306
           H D +     G E+ + +Y +KWPL L+  ++ L++
Sbjct: 602 HADPTELALSGLEAFSFDYIVKWPLSLIINRKALTR 637


>sp|P13267|DPO3_BACSU DNA polymerase III polC-type OS=Bacillus
           subtilis GN=polC PE=1 SV=2
          Length = 1437

 Score = 31.2 bits (69), Expect = 3.4
 Identities = 22/98 (22%), Positives = 43/98 (43%)
 Frame = -1

Query: 524 KACLCNNIHGQHTLKAPTKIYDTLHTCAIKKTSTLPTLPASMDCHLVEISEIKKLQHHCK 345
           KA    + H Q     P +IYDTL T   +  + +  + +S++    E+SE   +Q +  
Sbjct: 41  KASKSWHFHFQFKSLLPFQIYDTLTTRLTQSFAHIAKVTSSIEVQDAEVSE-SIVQDYWS 99

Query: 344 RCLTRFNKGASPYFDKVSLVKSSCRGHLMQYSNAMDSQ 231
           RC+      + P    ++  K   +G+ +      D++
Sbjct: 100 RCIEELQGISPPIISLLNQQKPKLKGNKLIVKTKTDTE 137
tr_hit_id A9NW25
Definition tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 72
Score (bit) 61.2
E-value 3.0e-08
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP913034|Adiantum capillus-veneris mRNA, clone:
YMU001_000025_F09.
         (548 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea...    61   3e-08
tr|Q60F24|Q60F24_ORYSJ Os05g0154500 protein OS=Oryza sativa subs...    50   6e-05
tr|B8AY65|B8AY65_ORYSI Putative uncharacterized protein OS=Oryza...    50   6e-05
tr|A3B039|A3B039_ORYSJ Putative uncharacterized protein OS=Oryza...    50   6e-05
tr|A9RXH9|A9RXH9_PHYPA Gamma tubulin ring complex protein 4 OS=P...    49   1e-04
tr|A7Q0E6|A7Q0E6_VITVI Chromosome chr7 scaffold_42, whole genome...    46   0.001
tr|A5BKC3|A5BKC3_VITVI Putative uncharacterized protein OS=Vitis...    46   0.001
tr|Q642S3|Q642S3_XENLA MGC81229 protein OS=Xenopus laevis GN=tub...    37   0.52 
tr|A7GME9|A7GME9_BACCN DNA topoisomerase I OS=Bacillus cereus su...    36   1.2  
tr|Q05AR5|Q05AR5_XENTR Tubulin, gamma complex associated protein...    36   1.5  
tr|Q8BYN2|Q8BYN2_MOUSE Putative uncharacterized protein OS=Mus m...    36   1.5  
tr|Q8BWR3|Q8BWR3_MOUSE Putative uncharacterized protein OS=Mus m...    36   1.5  
tr|Q8BKL6|Q8BKL6_MOUSE Putative uncharacterized protein OS=Mus m...    36   1.5  
tr|B6L8S9|B6L8S9_BRAFL Putative uncharacterized protein OS=Branc...    35   2.0  
tr|B3RSJ9|B3RSJ9_TRIAD Putative uncharacterized protein OS=Trich...    35   2.0  
tr|B3M608|B3M608_DROAN GF23778 OS=Drosophila ananassae GN=GF2377...    35   2.0  
tr|Q74YQ6|Q74YQ6_YERPE Putative membrane protein OS=Yersinia pes...    35   2.6  
tr|Q543T6|Q543T6_MOUSE Putative uncharacterized protein OS=Mus m...    35   3.4  
tr|Q3U6E9|Q3U6E9_MOUSE Putative uncharacterized protein OS=Mus m...    35   3.4  
tr|Q3TSV8|Q3TSV8_MOUSE Putative uncharacterized protein (Fragmen...    35   3.4  
tr|A2YWR4|A2YWR4_ORYSI Putative uncharacterized protein OS=Oryza...    34   4.4  
tr|Q5NVE4|Q5NVE4_PONAB Putative uncharacterized protein DKFZp459...    34   4.4  
tr|B7NZE4|B7NZE4_RABIT Tubulin, gamma complex associated protein...    34   4.4  
tr|B2KIP3|B2KIP3_RHIFE Gamma-tubulin complex component 4 (Predic...    34   4.4  
tr|B0KWF7|B0KWF7_CALJA Tubulin, gamma complex associated protein...    34   4.4  
tr|A9X1B2|A9X1B2_PAPAN Tubulin, gamma complex associated protein...    34   4.4  
tr|A0JNH5|A0JNH5_BOVIN Tubulin, gamma complex associated protein...    34   4.4  
tr|B6MLL6|B6MLL6_BRAFL Putative uncharacterized protein OS=Branc...    34   4.4  
tr|Q9BVR6|Q9BVR6_HUMAN TUBGCP4 protein OS=Homo sapiens GN=TUBGCP...    34   4.4  
tr|B3KNK6|B3KNK6_HUMAN cDNA FLJ14797 fis, clone NT2RP4001256, hi...    34   4.4  

>tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 754

 Score = 61.2 bits (147), Expect = 3e-08
 Identities = 31/72 (43%), Positives = 49/72 (68%), Gaps = 2/72 (2%)
 Frame = +1

Query: 97  VDFRVSSVSVLGASHKEILRPRSSAEGNIRLLT-IHGDISNKLN-DGWESIALEYCIKWP 270
           V  R+ S+     +H +I RP+S+ +G++  L+ ++G+ S   + DGW+ IALEY + WP
Sbjct: 466 VSLRMPSLGSTSTTHGDISRPKSTRDGSLGSLSNMYGNSSAGPSLDGWDGIALEYSVDWP 525

Query: 271 LQLLFTKETLSK 306
           LQLLFT+E L+K
Sbjct: 526 LQLLFTREVLAK 537


>tr|Q60F24|Q60F24_ORYSJ Os05g0154500 protein OS=Oryza sativa subsp.
           japonica GN=OSJNBa0059G01.11 PE=4 SV=1
          Length = 754

 Score = 50.4 bits (119), Expect = 6e-05
 Identities = 27/71 (38%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
 Frame = +1

Query: 97  VDFRVSSVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 273
           V  R+SS  +  ++ ++ L+  S+ E     ++  G  +++L  DGW+SIALEY + WPL
Sbjct: 469 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 523

Query: 274 QLLFTKETLSK 306
           QL FT + +SK
Sbjct: 524 QLFFTPDVVSK 534


>tr|B8AY65|B8AY65_ORYSI Putative uncharacterized protein OS=Oryza
           sativa subsp. indica GN=OsI_18509 PE=4 SV=1
          Length = 762

 Score = 50.4 bits (119), Expect = 6e-05
 Identities = 27/71 (38%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
 Frame = +1

Query: 97  VDFRVSSVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 273
           V  R+SS  +  ++ ++ L+  S+ E     ++  G  +++L  DGW+SIALEY + WPL
Sbjct: 469 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 523

Query: 274 QLLFTKETLSK 306
           QL FT + +SK
Sbjct: 524 QLFFTPDVVSK 534


>tr|A3B039|A3B039_ORYSJ Putative uncharacterized protein OS=Oryza
           sativa subsp. japonica GN=OsJ_016411 PE=4 SV=1
          Length = 700

 Score = 50.4 bits (119), Expect = 6e-05
 Identities = 27/71 (38%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
 Frame = +1

Query: 97  VDFRVSSVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 273
           V  R+SS  +  ++ ++ L+  S+ E     ++  G  +++L  DGW+SIALEY + WPL
Sbjct: 407 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 461

Query: 274 QLLFTKETLSK 306
           QL FT + +SK
Sbjct: 462 QLFFTPDVVSK 472


>tr|A9RXH9|A9RXH9_PHYPA Gamma tubulin ring complex protein 4
           OS=Physcomitrella patens subsp. patens
           GN=PHYPADRAFT_206582 PE=4 SV=1
          Length = 735

 Score = 49.3 bits (116), Expect = 1e-04
 Identities = 20/27 (74%), Positives = 23/27 (85%)
 Frame = +1

Query: 226 DGWESIALEYCIKWPLQLLFTKETLSK 306
           DGW+ +ALEY I WPLQLLFTK+ LSK
Sbjct: 502 DGWDGLALEYSINWPLQLLFTKDVLSK 528


>tr|A7Q0E6|A7Q0E6_VITVI Chromosome chr7 scaffold_42, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00028020001
           PE=4 SV=1
          Length = 743

 Score = 45.8 bits (107), Expect = 0.001
 Identities = 18/27 (66%), Positives = 22/27 (81%)
 Frame = +1

Query: 226 DGWESIALEYCIKWPLQLLFTKETLSK 306
           +GW+ IALEY + WPLQL FT+E LSK
Sbjct: 501 EGWDGIALEYSVDWPLQLFFTQEVLSK 527


>tr|A5BKC3|A5BKC3_VITVI Putative uncharacterized protein OS=Vitis
            vinifera GN=VITISV_003625 PE=4 SV=1
          Length = 1529

 Score = 45.8 bits (107), Expect = 0.001
 Identities = 18/27 (66%), Positives = 22/27 (81%)
 Frame = +1

Query: 226  DGWESIALEYCIKWPLQLLFTKETLSK 306
            +GW+ IALEY + WPLQL FT+E LSK
Sbjct: 1287 EGWDGIALEYSVDWPLQLFFTQEVLSK 1313


>tr|Q642S3|Q642S3_XENLA MGC81229 protein OS=Xenopus laevis
           GN=tubgcp4 PE=2 SV=1
          Length = 666

 Score = 37.4 bits (85), Expect = 0.52
 Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
 Frame = +1

Query: 130 GASHKEILRPRSSAEGNIRLLTIHGDISNKL--NDGWESIALEYCIKWPLQLLFTKETLS 303
           G  HK+  +PR   EG  R      D+S +     GW ++ L Y ++WPL +LFT   L 
Sbjct: 422 GKEHKDTSQPR---EGPFR------DMSPREAPTSGWAALGLSYKVQWPLHILFTPAVLE 472

Query: 304 K 306
           K
Sbjct: 473 K 473


>tr|A7GME9|A7GME9_BACCN DNA topoisomerase I OS=Bacillus cereus
           subsp. cytotoxis (strain NVH 391-98) GN=Bcer98_0977 PE=4
           SV=1
          Length = 96

 Score = 36.2 bits (82), Expect = 1.2
 Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
 Frame = -1

Query: 377 SEIKKLQ--HHCKRCLTRFNKGASPYFDKVSLVKSSCRGHLMQYSNAMDSQPSFSLFEI 207
           +EI KL   HH ++ ++     + PY++    + S CRGHL++  N  +  P + +F++
Sbjct: 22  NEIMKLPRFHHSQKYIS-----SKPYYENNHYIVSWCRGHLLELKNPEEMDPMYKVFKL 75


>tr|Q05AR5|Q05AR5_XENTR Tubulin, gamma complex associated protein 4
           OS=Xenopus tropicalis GN=tubgcp4 PE=2 SV=1
          Length = 665

 Score = 35.8 bits (81), Expect = 1.5
 Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
 Frame = +1

Query: 130 GASHKEILRPRSSAEGNIRLLTIHGDISNKLN--DGWESIALEYCIKWPLQLLFTKETLS 303
           G  HK++ +PR   EG  R      D S +     GW ++ L Y ++WPL +LFT   L 
Sbjct: 421 GKEHKDMSQPR---EGPSR------DTSPREPPISGWAALGLSYKVQWPLHILFTPAVLE 471

Query: 304 K 306
           K
Sbjct: 472 K 472