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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
BP913149
Clone id YMU001_000027_A08
Library
Length 397
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000027_A08.
Accession
Tissue type prothallium
Developmental stage -
Contig ID -
Sequence 
CCTCTTTACTGCAAGAACAGAAACTTCTTTAACTGACACTCCACAAGGAAAAGAATCCAC
ATTTAGCAACTTTATCAAGGTAGGAATGGCATCCTGTATGCTGTCCTCAGTGATAAAATT
TGCTAGCTTGGCAAGCAACAAACAACCCAACATTTGCATGGCCGGATCTTCAAAATTGAT
CATTTGAACCAATAAAAGGTCACAATCAGCGGTCAGGCTCTCATCAAATCCATCTTTCAT
TGCCATCTCAAGGCCGGCTTTCATAAACTGTCTTCTAGTAAATCTGTCGAAAACACCATC
ATTTGAGGCTGACACAAAAGCCGCCCTGACTTTTGCGTAGTGACTATCACAAGTCTCTTC
CAATATGCACCCTTCATCTAGCCACTTTCTTATCACA
■■Homology search results ■■ -
sp_hit_id Q55BP5
Definition sp|Q55BP5|TOP2_DICDI Probable DNA topoisomerase 2 OS=Dictyostelium discoideum
Align length 62
Score (bit) 30.8
E-value 2.2
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP913149|Adiantum capillus-veneris mRNA, clone:
YMU001_000027_A08.
         (397 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q55BP5|TOP2_DICDI Probable DNA topoisomerase 2 OS=Dictyosteli...    31   2.2  
sp|Q8DIN5|PUR9_THEEB Bifunctional purine biosynthesis protein pu...    31   2.2  
sp|P34534|TOP2M_CAEEL Putative DNA topoisomerase 2, mitochondria...    30   2.9  
sp|Q01879|TOP2_PICGU DNA topoisomerase 2 OS=Pichia guilliermondi...    30   3.7  
sp|Q833Z3|PUR9_ENTFA Bifunctional purine biosynthesis protein pu...    30   3.7  
sp|Q6BYZ4|PSF3_DEBHA DNA replication complex GINS protein PSF3 O...    30   3.7  
sp|Q7VWD8|MSBA_BORPE Lipid A export ATP-binding/permease protein...    30   3.7  
sp|Q7W9N7|MSBA_BORPA Lipid A export ATP-binding/permease protein...    30   3.7  
sp|Q7WH20|MSBA_BORBR Lipid A export ATP-binding/permease protein...    30   3.7  
sp|Q60T36|SRA11_CAEBR Serpentine receptor class alpha-11 OS=Caen...    30   4.9  
sp|P35084|RPB1_DICDI DNA-directed RNA polymerase II subunit rpb1...    29   6.4  
sp|P10081|IF4A_YEAST ATP-dependent RNA helicase eIF4A OS=Sacchar...    29   6.4  
sp|A6ZQJ1|IF4A_YEAS7 ATP-dependent RNA helicase eIF4A OS=Sacchar...    29   6.4  
sp|O63623|NU1M_DALCH NADH-ubiquinone oxidoreductase chain 1 OS=D...    29   6.4  
sp|P87078|TOP2_CANAL DNA topoisomerase 2 OS=Candida albicans GN=...    29   8.3  

>sp|Q55BP5|TOP2_DICDI Probable DNA topoisomerase 2 OS=Dictyostelium
           discoideum GN=top2 PE=3 SV=1
          Length = 1521

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 20/62 (32%), Positives = 31/62 (50%)
 Frame = +2

Query: 191 NKRSQSAVRLSSNPSFIAISRPAFINCLLVNLSKTPSFEADTKAALTFA**LSQVSSNMH 370
           NK+++ +  +   P+FI      F+NCL+ N    P F++ TK  LT    +S   S   
Sbjct: 412 NKKNKGSADIK--PAFIKNHLSVFVNCLIEN----PHFDSQTKETLTSK--ISSFGSRCE 463

Query: 371 PS 376
           PS
Sbjct: 464 PS 465


>sp|Q8DIN5|PUR9_THEEB Bifunctional purine biosynthesis protein purH
           OS=Thermosynechococcus elongatus (strain BP-1) GN=purH
           PE=3 SV=1
          Length = 518

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 19/73 (26%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
 Frame = +2

Query: 44  QGKESTFSNFIKVGMASCMLSSVIKFASLASN--KQPNICMAGSSKLII*TNKRSQSAVR 217
           QGKE +++N + +  A  ++S  +  +  A+   K  N C     K ++   +R+ +A R
Sbjct: 247 QGKELSYNNLLDLEAARGVISEFLGDSPPAAVIIKHTNPCGVAEGKTLVEAYERAFAADR 306

Query: 218 LSSNPSFIAISRP 256
           +S+    +A++RP
Sbjct: 307 VSAFGGIVALNRP 319


>sp|P34534|TOP2M_CAEEL Putative DNA topoisomerase 2, mitochondrial
           OS=Caenorhabditis elegans GN=R05D3.1 PE=2 SV=4
          Length = 1054

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 17/37 (45%), Positives = 21/37 (56%)
 Frame = +2

Query: 221 SSNPSFIAISRPAFINCLLVNLSKTPSFEADTKAALT 331
           S  P+ I  +   FINCL+ N    PSFE+ TK  LT
Sbjct: 254 SVKPAVIKNNLSIFINCLIEN----PSFESQTKETLT 286


>sp|Q01879|TOP2_PICGU DNA topoisomerase 2 OS=Pichia guilliermondii
           GN=TOP2 PE=3 SV=3
          Length = 1400

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 18/54 (33%), Positives = 28/54 (51%)
 Frame = +2

Query: 170 SKLII*TNKRSQSAVRLSSNPSFIAISRPAFINCLLVNLSKTPSFEADTKAALT 331
           +KLI    K+ +   +L+  P  +  +   FINCL+ N    P+F + TK  LT
Sbjct: 372 TKLIDALTKKEKGKKKLNIKPQEVRNNMFVFINCLIEN----PAFTSQTKEQLT 421


>sp|Q833Z3|PUR9_ENTFA Bifunctional purine biosynthesis protein purH
           OS=Enterococcus faecalis GN=purH PE=3 SV=1
          Length = 513

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 24/101 (23%), Positives = 50/101 (49%), Gaps = 6/101 (5%)
 Frame = +2

Query: 47  GKESTFSNFIKVGMASCMLSSVIKFASLASNKQPNICMAGSSKLII*TNKRSQSAVRLSS 226
           GKE +++N I+   A+  ++S     ++ + K  N C  G+ K I+   +++  A  +S 
Sbjct: 243 GKELSYNN-IRDADAALRIASEFTEPTVVAVKHMNPCGIGTGKTILAAYRQAFEADPVSI 301

Query: 227 NPSFIAISRP------AFINCLLVNLSKTPSFEADTKAALT 331
               + ++RP      A ++ + + +   PSFE +  A L+
Sbjct: 302 FGGIVVLNRPVDQATAAEMHQIFLEIIIAPSFEEEALALLS 342


>sp|Q6BYZ4|PSF3_DEBHA DNA replication complex GINS protein PSF3
           OS=Debaryomyces hansenii GN=PSF3 PE=3 SV=2
          Length = 176

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = +2

Query: 224 SNPSFIAISRPAFINCLLVNLSKTPSFEADTKAALT 331
           S  SFI +S+P FIN  ++N  KT +   D  + LT
Sbjct: 65  SQQSFIDLSQPDFINYKVINAIKTNAVNIDLHSILT 100


>sp|Q7VWD8|MSBA_BORPE Lipid A export ATP-binding/permease protein
           msbA OS=Bordetella pertussis GN=msbA PE=3 SV=1
          Length = 623

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -3

Query: 371 GAYWKRLVIVTTQKSGRLLCQPQMMVFSTDLLED 270
           G+YWK LV+     +G    QP + V    LL+D
Sbjct: 60  GSYWKGLVLAVLLMAGAAATQPTLAVIMKPLLDD 93


>sp|Q7W9N7|MSBA_BORPA Lipid A export ATP-binding/permease protein
           msbA OS=Bordetella parapertussis GN=msbA PE=3 SV=1
          Length = 623

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -3

Query: 371 GAYWKRLVIVTTQKSGRLLCQPQMMVFSTDLLED 270
           G+YWK LV+     +G    QP + V    LL+D
Sbjct: 60  GSYWKGLVLAVLLMAGAAATQPTLAVIMKPLLDD 93


>sp|Q7WH20|MSBA_BORBR Lipid A export ATP-binding/permease protein
           msbA OS=Bordetella bronchiseptica GN=msbA PE=3 SV=2
          Length = 623

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -3

Query: 371 GAYWKRLVIVTTQKSGRLLCQPQMMVFSTDLLED 270
           G+YWK LV+     +G    QP + V    LL+D
Sbjct: 60  GSYWKGLVLAVLLMAGAAATQPTLAVIMKPLLDD 93


>sp|Q60T36|SRA11_CAEBR Serpentine receptor class alpha-11
           OS=Caenorhabditis briggsae GN=sra-11 PE=3 SV=1
          Length = 343

 Score = 29.6 bits (65), Expect = 4.9
 Identities = 16/86 (18%), Positives = 37/86 (43%), Gaps = 4/86 (4%)
 Frame = +2

Query: 41  PQGKESTFSNFIKVGM----ASCMLSSVIKFASLASNKQPNICMAGSSKLII*TNKRSQS 208
           P      F  F+++ +    A  +++ +I + ++  +++  +            N+R QS
Sbjct: 182 PPKSVGRFQKFLEIALYMSLAHMVINVIILYINVVQDRRQRLVSTHDQSQSFDVNQRFQS 241

Query: 209 AVRLSSNPSFIAISRPAFINCLLVNL 286
            V L S  +   +S   F++C L  +
Sbjct: 242 RVALKSTQAIFFLSMSQFLSCFLYTI 267
tr_hit_id Q20Y40
Definition tr|Q20Y40|Q20Y40_RHOPB Putative uncharacterized protein OS=Rhodopseudomonas palustris (strain BisB18)
Align length 106
Score (bit) 35.4
E-value 1.4
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP913149|Adiantum capillus-veneris mRNA, clone:
YMU001_000027_A08.
         (397 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q20Y40|Q20Y40_RHOPB Putative uncharacterized protein OS=Rhodo...    35   1.4  
tr|A6GIK7|A6GIK7_9DELT Putative uncharacterized protein OS=Plesi...    33   5.4  
tr|Q6DTM8|Q6DTM8_CANGA Cell wall mannoprotein OS=Candida glabrat...    33   5.4  
tr|B4JUS7|B4JUS7_DROGR GH15123 OS=Drosophila grimshawi GN=GH1512...    33   7.0  
tr|B7QQL5|B7QQL5_9RHOB RND transporter, HAE1/HME family, permeas...    33   7.1  
tr|Q13IE9|Q13IE9_BURXL Putative uncharacterized protein OS=Burkh...    33   9.2  
tr|A8UE01|A8UE01_9FLAO Enoyl-CoA hydratase/isomerase OS=Flavobac...    33   9.2  
tr|B2BD27|B2BD27_BIOGL Fibrinogen-related protein 14 OS=Biomphal...    33   9.2  
tr|A9V1V6|A9V1V6_MONBE Predicted protein OS=Monosiga brevicollis...    33   9.2  

>tr|Q20Y40|Q20Y40_RHOPB Putative uncharacterized protein
           OS=Rhodopseudomonas palustris (strain BisB18)
           GN=RPC_4423 PE=4 SV=1
          Length = 196

 Score = 35.4 bits (80), Expect = 1.4
 Identities = 30/106 (28%), Positives = 47/106 (44%)
 Frame = -2

Query: 369 CILEETCDSHYAKVRAAFVSASNDGVFDRFTRRQFMKAGLEMAMKDGFDESLTADCDLLL 190
           CIL+ET        RA     S+ GV D   R    +A +E A    F+E++  D D+ L
Sbjct: 40  CILDET-------QRAIEKILSDKGVPDAADRAARARASMETA----FEEAMVTDFDVFL 88

Query: 189 VQMINFEDPAMQMLGCLLLAKLANFITEDSIQDAIPTLIKLLNVDS 52
                  DP    +    L   A  I  D+++D    ++K LN+++
Sbjct: 89  PASGGLPDPGDAHVLAAALKIQAAMIVTDNLKDFPENVLKRLNIEA 134


>tr|A6GIK7|A6GIK7_9DELT Putative uncharacterized protein
            OS=Plesiocystis pacifica SIR-1 GN=PPSIR1_09301 PE=4 SV=1
          Length = 995

 Score = 33.5 bits (75), Expect = 5.4
 Identities = 36/131 (27%), Positives = 51/131 (38%), Gaps = 25/131 (19%)
 Frame = -2

Query: 381  LDEGCILEETCDSHYAKVRAAFVSASNDGVFD-----RFTRRQFMKAGLEMAMKDGFD-- 223
            LD+    +   ++ Y+KVR  F               R   R F +AG+EM  + GF+  
Sbjct: 709  LDDDSAFKHNAEAPYSKVRLFFAKVGTLRFMSQLDLVRIIPRMFRRAGIEMGFRRGFNPQ 768

Query: 222  ---------------ESLTADCDLLLVQMINFEDPAMQMLGCLLLAKLANFITED---SI 97
                           +    DCDLLL      E  A  M G LL A+    I +D    +
Sbjct: 769  PRMSFGPALALGMGADEEVVDCDLLL------ERSAEDMAG-LLSAEERQAIADDVFARL 821

Query: 96   QDAIPTLIKLL 64
            QD+ P  + LL
Sbjct: 822  QDSTPPGMVLL 832


>tr|Q6DTM8|Q6DTM8_CANGA Cell wall mannoprotein OS=Candida glabrata
           GN=DAN1 PE=4 SV=1
          Length = 376

 Score = 33.5 bits (75), Expect = 5.4
 Identities = 27/104 (25%), Positives = 49/104 (47%), Gaps = 1/104 (0%)
 Frame = +2

Query: 17  TETSLTDTPQGKESTFSNFIKVGMASCMLSSVIKFASLASNKQPNICMAGSSKLII*TNK 196
           T  S +D+    +STFS+F+    ++ + SSV    S +S+   +     SS     ++ 
Sbjct: 92  TSISSSDSTASSDSTFSSFVSSSSSTSVTSSVSSSTSASSSTSASSSTFASSSTSASSST 151

Query: 197 RSQSAVRLSSNPSFIAISRPAFINCL-LVNLSKTPSFEADTKAA 325
            + S+   SS+ S  A S   +++ L  V+ S +PS    +  A
Sbjct: 152 SASSSTSASSSTS--ASSSSLYVSALSSVSASSSPSSHTSSVVA 193


>tr|B4JUS7|B4JUS7_DROGR GH15123 OS=Drosophila grimshawi GN=GH15123
           PE=4 SV=1
          Length = 990

 Score = 33.1 bits (74), Expect = 7.0
 Identities = 27/85 (31%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
 Frame = +2

Query: 65  SNFIKVGMASCMLSSVIKFASLASNKQPNICMAGSSKLII*TNKRSQSAVRLSSNPSFIA 244
           S+ +    ASC  SS + +    +N      +AGSS      ++ S S++ LSSNP+ + 
Sbjct: 180 SSSLASAAASCSTSSAVTYGG-GANSAAAALIAGSSPF---GSQSSTSSLSLSSNPANVT 235

Query: 245 ISRPAFINCLLVNLSK-TPSFEADT 316
            +  A      V +SK TPS  A T
Sbjct: 236 ATTTAAAGASTVTMSKSTPSTTAAT 260


>tr|B7QQL5|B7QQL5_9RHOB RND transporter, HAE1/HME family, permease
           protein OS=Ruegeria sp. R11 GN=RR11_2468 PE=4 SV=1
          Length = 1258

 Score = 33.1 bits (74), Expect = 7.1
 Identities = 18/54 (33%), Positives = 27/54 (50%)
 Frame = -3

Query: 377 MKGAYWKRLVIVTTQKSGRLLCQPQMMVFSTDLLEDSL*KPALRWQ*KMDLMRA 216
           +KG  W+ L   T Q   +    P +     DL+EDSL  P + WQ  +D+ +A
Sbjct: 769 IKGDNWETLTAATAQARAKFEATPGL-----DLIEDSLPLPGIDWQIDVDVEKA 817


>tr|Q13IE9|Q13IE9_BURXL Putative uncharacterized protein
           OS=Burkholderia xenovorans (strain LB400) GN=Bxeno_C0212
           PE=4 SV=1
          Length = 346

 Score = 32.7 bits (73), Expect = 9.2
 Identities = 18/49 (36%), Positives = 29/49 (59%), Gaps = 5/49 (10%)
 Frame = -2

Query: 276 RRQFMKAGLEMAMKDGFDESLTA--DCDLLLVQMINFEDP---AMQMLG 145
           ++ ++++ + + MKDGF  S+    D DLL   M +FEDP   AM  +G
Sbjct: 212 QKSYIRSEIRLVMKDGFAVSIEGGMDADLLSEYMASFEDPEAYAMSHIG 260


>tr|A8UE01|A8UE01_9FLAO Enoyl-CoA hydratase/isomerase
           OS=Flavobacteriales bacterium ALC-1 GN=FBALC1_14187 PE=4
           SV=1
          Length = 250

 Score = 32.7 bits (73), Expect = 9.2
 Identities = 24/57 (42%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
 Frame = -2

Query: 189 VQMINFEDPAMQMLGCLLLAKLANFITEDSIQDAIPTLIKLLNVD-SFPCGVSVKEV 22
           V  I F  PA   L   +LAKLAN ITE    DAI  ++     D +F  G S KE+
Sbjct: 14  VGYIEFFHPAHNSLPGDILAKLANTITEAGKNDAIKVIVLKSGGDRTFCAGASFKEL 70


>tr|B2BD27|B2BD27_BIOGL Fibrinogen-related protein 14
           OS=Biomphalaria glabrata PE=2 SV=1
          Length = 399

 Score = 32.7 bits (73), Expect = 9.2
 Identities = 15/59 (25%), Positives = 30/59 (50%)
 Frame = +1

Query: 4   LYCKNRNFFN*HSTRKRIHI*QLYQGRNGILYAVLSDKIC*LGKQQTTQHLHGRIFKID 180
           L C  ++F N  +   ++HI  +    NG++  +  D+   +   Q++ H HG+I+  D
Sbjct: 42  LSCSLKSFNNETNEHFKVHIMFIQHETNGVISTLSKDQAVAVSADQSSTHAHGKIYNKD 100


>tr|A9V1V6|A9V1V6_MONBE Predicted protein OS=Monosiga brevicollis
            GN=37458 PE=4 SV=1
          Length = 1605

 Score = 32.7 bits (73), Expect = 9.2
 Identities = 26/90 (28%), Positives = 41/90 (45%), Gaps = 9/90 (10%)
 Frame = +2

Query: 8    TARTETSLTDTPQGKESTFSNFIKVGMASCM-LSSVIKFASLASNKQPNI--------CM 160
            T  T T+ T T     +T +     G A C  ++S   F+SL +NKQ  +        C 
Sbjct: 1150 TTTTTTTTTTTTTTTTTTTTLLTATGPAYCANINSQTVFSSLLNNKQRILDGIFLLTGCQ 1209

Query: 161  AGSSKLII*TNKRSQSAVRLSSNPSFIAIS 250
            AG+S L + +   S   + L  NPS  +++
Sbjct: 1210 AGNSVLTVNSLIESIGPITLEGNPSVTSLA 1239