BP913435
Clone id YMU001_000030_C02
Library
Length 546
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000030_C02.
Accession
Tissue type prothallium
Developmental stage -
Contig ID
Sequence
AACTGGAGGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTACTAAACACACTGCAGT
TCTCATTATGAATGGGTAAAGCTCTACTAGATAGTTTTCATCCAATTCGTAACGCCGTTC
ATTCAAAGCCTCTTCTTGCAAGCTCCATTATGTCACATGAACGGTTTAGTAGACAATCGA
ATGAATTCATCAAAGTCTGGATGCACGTGCAAGGCTTTCTGGCACTCCTTCTAGACTTCC
ATGCAAGAGTTCCCGAGCTTCCCACTTGAACACTTCTTCCTTTAATGCTCTGTAGACACC
GCGATCATAATTTCTGGAAGCTAATTCAGATGCTTTCTTGATAGCTGTTTCCCGGGTGGA
GTCTACATCAGAGCACGCCCCATGTATTATTCCTGCAACGGCTGCAGTCTTCCCTACATA
CCGATGAGCCGTTAGCACTGCTTCATGGTAGGTTGGCACTGGGAGCTTGCTCTTGATGAT
TGCATTCATACCAGGAGTTAAAGGAAGCTTGATTTCTCATTCAACATTATAAACCCTCTG
TCGCTT
■■Homology search results ■■ -
sp_hit_id A0JXZ9
Definition sp|A0JXZ9|ILVD_ARTS2 Dihydroxy-acid dehydratase OS=Arthrobacter sp. (strain FB24)
Align length 55
Score (bit) 30.8
E-value 3.7
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP913435|Adiantum capillus-veneris mRNA, clone:
YMU001_000030_C02.
(503 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|A0JXZ9|ILVD_ARTS2 Dihydroxy-acid dehydratase OS=Arthrobacter ... 31 3.7
sp|Q5Z0M2|ILVD1_NOCFA Dihydroxy-acid dehydratase 1 OS=Nocardia f... 31 3.7
sp|A8MLD3|RPOC_ALKOO DNA-directed RNA polymerase subunit beta' O... 30 4.9
sp|Q17X65|NADE_HELAH NH(3)-dependent NAD(+) synthetase OS=Helico... 30 4.9
sp|A3PSS2|ILVD_MYCSJ Dihydroxy-acid dehydratase OS=Mycobacterium... 30 4.9
sp|Q06FX0|RPOC1_PELHO DNA-directed RNA polymerase subunit beta' ... 30 6.4
sp|P24043|LAMA2_HUMAN Laminin subunit alpha-2 OS=Homo sapiens GN... 30 8.3

>sp|A0JXZ9|ILVD_ARTS2 Dihydroxy-acid dehydratase OS=Arthrobacter sp.
(strain FB24) GN=ilvD PE=3 SV=1
Length = 573

Score = 30.8 bits (68), Expect = 3.7
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = -1

Query: 353 AGIIHGACSDVDSTRETAIKKASELASRNYDRGVYRALKEEVFKWEARELLHGSL 189
AG++HG C V T +T + + + + D + RAL + K +LHGS+
Sbjct: 351 AGLLHGDCLTV--TGKTLAENLASINPPDLDGKILRALDNPIHKTGGITILHGSM 403


>sp|Q5Z0M2|ILVD1_NOCFA Dihydroxy-acid dehydratase 1 OS=Nocardia
farcinica GN=ilvD1 PE=3 SV=1
Length = 568

Score = 30.8 bits (68), Expect = 3.7
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -1

Query: 368 KTAAVAGIIHGACSDVDSTRETAIKKASELASRNYDRGVYRALKEEVFKWEARELLHGSL 189
KT AG++HG C V T T + + +A + D V RAL + + +L GSL
Sbjct: 341 KTLLDAGLLHGDCLTV--TGRTVAENLAHIAPPDPDGKVVRALNDPIHPTGGITILSGSL 398


>sp|A8MLD3|RPOC_ALKOO DNA-directed RNA polymerase subunit beta'
OS=Alkaliphilus oremlandii (strain OhILAs) GN=rpoC PE=3
SV=1
Length = 1179

Score = 30.4 bits (67), Expect = 4.9
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1

Query: 332 CSDVDSTRETAIKKASELASRNYDRGVYRALKEEVFKWEARELLHGSLEGVPESLA 165
C + + A+K +E+ YDR V R EEV E E++ G E + E +A
Sbjct: 791 CGSQEGSNIKALKDGNEVIEELYDRIVGRYTLEEVIHPETGEVMIGKNEMILEDMA 846


>sp|Q17X65|NADE_HELAH NH(3)-dependent NAD(+) synthetase
OS=Helicobacter acinonychis (strain Sheeba) GN=nadE PE=3
SV=1
Length = 260

Score = 30.4 bits (67), Expect = 4.9
Identities = 23/63 (36%), Positives = 29/63 (46%)
Frame = -1

Query: 314 TRETAIKKASELASRNYDRGVYRALKEEVFKWEARELLHGSLEGVPESLARASRL**IHS 135
T++ KK S D V L ++VFK A LL SL +PES + A L S
Sbjct: 20 TQKRGFKKVVYGLSGGLDSAVVGVLSQKVFKENAHALLMPSLVSMPESKSDALDLCETFS 79

Query: 134 IVY 126
I Y
Sbjct: 80 IPY 82


>sp|A3PSS2|ILVD_MYCSJ Dihydroxy-acid dehydratase OS=Mycobacterium
sp. (strain JLS) GN=ilvD PE=3 SV=1
Length = 580

Score = 30.4 bits (67), Expect = 4.9
Identities = 20/62 (32%), Positives = 29/62 (46%)
Frame = -1

Query: 374 VGKTAAVAGIIHGACSDVDSTRETAIKKASELASRNYDRGVYRALKEEVFKWEARELLHG 195
V K AG++HG C V T ET + + + + D V RA+ + +LHG
Sbjct: 351 VMKALLDAGLMHGDCLTV--TGETMAENLAHIEPPDPDGKVLRAMNNPIHPTGGITILHG 408

Query: 194 SL 189
SL
Sbjct: 409 SL 410


>sp|Q06FX0|RPOC1_PELHO DNA-directed RNA polymerase subunit beta'
OS=Pelargonium hortorum GN=rpoC1 PE=3 SV=1
Length = 701

Score = 30.0 bits (66), Expect = 6.4
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = -1

Query: 500 RQRVYNVE*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGI 345
RQ+ N++ L +T G + I+ +LPV ++E++ T H G V +
Sbjct: 616 RQKRINLDSPFWLKVTWGQHRIVSKELPVEVHYESLGTHHEIYGHCKIVRSV 667


>sp|P24043|LAMA2_HUMAN Laminin subunit alpha-2 OS=Homo sapiens
GN=LAMA2 PE=1 SV=4
Length = 3122

Score = 29.6 bits (65), Expect = 8.3
Identities = 20/71 (28%), Positives = 29/71 (40%)
Frame = -3

Query: 324 CRLHPGNSYQESI*ISFQKL*SRCLQSIKGRSVQVGSSGTLAWKSRRSARKPCTCIQTLM 145
CR + G S+ E Q C +++G+ +GT +S R PC C
Sbjct: 918 CRCNAGGSFSEVC--HSQTGQCECRANVQGQRCDKCKAGTFGLQSARGC-VPCNCNSFGS 974

Query: 144 NSFDCLLNRSC 112
SFDC + C
Sbjct: 975 KSFDCEESGQC 985


tr_hit_id A9RRB0
Definition tr|A9RRB0|A9RRB0_PHYPA Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens
Align length 85
Score (bit) 101.0
E-value 2.0e-20
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP913435|Adiantum capillus-veneris mRNA, clone:
YMU001_000030_C02.
(503 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9RRB0|A9RRB0_PHYPA Predicted protein (Fragment) OS=Physcomit... 101 2e-20
tr|Q54SN3|Q54SN3_DICDI Putative uncharacterized protein OS=Dicty... 60 6e-08
tr|A0ACZ7|A0ACZ7_STRAM Putative enoyl-CoA hydratase/isomerase fa... 60 8e-08
tr|Q47PN9|Q47PN9_THEFY Putative enoyl-CoA hydratase/isomerase fa... 57 4e-07
tr|B6KFN8|B6KFN8_TOXGO Enoyl-CoA hydratase/isomerase family prot... 53 9e-06
tr|A0Z9E4|A0Z9E4_9GAMM Putative enoyl-CoA hydratase/isomerase fa... 51 4e-05
tr|A9NYU1|A9NYU1_PICSI Putative uncharacterized protein OS=Picea... 50 5e-05
tr|A8L469|A8L469_FRASN Enoyl-CoA hydratase/isomerase OS=Frankia ... 50 6e-05
tr|A8KYK2|A8KYK2_FRASN Enoyl-CoA hydratase/isomerase OS=Frankia ... 49 1e-04
tr|B1MBC5|B1MBC5_MYCA9 Putative enoyl-coa hydratase/isomerase fa... 47 7e-04
tr|A0YFY4|A0YFY4_9GAMM Putative enoyl-CoA hydratase/isomerase fa... 45 0.001
tr|Q0S9U5|Q0S9U5_RHOSR Possible enoyl-CoA hydratase OS=Rhodococc... 45 0.003
tr|Q9P3G4|Q9P3G4_NEUCR Putative uncharacterized protein B2A19.01... 43 0.007
tr|Q65XC3|Q65XC3_ORYSJ Os05g0529200 protein OS=Oryza sativa subs... 42 0.013
tr|A3B635|A3B635_ORYSJ Putative uncharacterized protein OS=Oryza... 42 0.013
tr|A2Y6S0|A2Y6S0_ORYSI Putative uncharacterized protein OS=Oryza... 42 0.013
tr|B6GYH9|B6GYH9_PENCH Pc12g01170 protein OS=Penicillium chrysog... 42 0.013
tr|A0C764|A0C764_PARTE Chromosome undetermined scaffold_154, who... 42 0.021
tr|B2B1W2|B2B1W2_PODAN Predicted CDS Pa_6_4650 OS=Podospora anse... 42 0.021
tr|Q9CB21|Q9CB21_MYCLE Possible enoyl-CoA hydratase OS=Mycobacte... 41 0.037
tr|Q0S351|Q0S351_RHOSR Possible enoyl-CoA hydratase OS=Rhodococc... 41 0.037
tr|B2GIB8|B2GIB8_KOCRD Putative enoyl-CoA hydratase OS=Kocuria r... 41 0.037
tr|B8ZTB9|B8ZTB9_MYCLE Possible enoyl-CoA hydratase OS=Mycobacte... 41 0.037
tr|B6TPA4|B6TPA4_MAIZE Carnitine racemase/ catalytic OS=Zea mays... 41 0.037
tr|Q1YQ28|Q1YQ28_9GAMM Putative enoyl-CoA hydratase/isomerase fa... 40 0.048
tr|Q5Z184|Q5Z184_NOCFA Putative enoyl-CoA hydratase/isomerase fa... 40 0.062
tr|A9NZH5|A9NZH5_PICSI Putative uncharacterized protein OS=Picea... 40 0.062
tr|A9NSP6|A9NSP6_PICSI Putative uncharacterized protein OS=Picea... 40 0.062
tr|Q5YVK1|Q5YVK1_NOCFA Putative enoyl-CoA hydratase/isomerase fa... 40 0.081
tr|Q5AY63|Q5AY63_EMENI Putative uncharacterized protein OS=Emeri... 40 0.081

>tr|A9RRB0|A9RRB0_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_26730 PE=4 SV=1
Length = 205

Score = 101 bits (252), Expect = 2e-20
Identities = 48/85 (56%), Positives = 67/85 (78%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E +IKLP +PGMNAII+ KLPV TYH+A+L+A RY G+TA AG++H + SD+ ST + A
Sbjct: 121 EIDIKLPFSPGMNAIIRCKLPVMTYHKAMLSAFRYTGQTAVEAGMVHSSPSDLQSTYQEA 180

Query: 299 IKKASELASRNYDRGVYRALKEEVF 225
+K A LASRN+DR VY+ +K+++F
Sbjct: 181 LKLAKGLASRNFDRVVYQTIKKQMF 205


>tr|Q54SN3|Q54SN3_DICDI Putative uncharacterized protein
OS=Dictyostelium discoideum GN=DDB_0204191 PE=4 SV=1
Length = 249

Score = 60.1 bits (144), Expect = 6e-08
Identities = 34/98 (34%), Positives = 60/98 (61%), Gaps = 1/98 (1%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPT-YHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRET 303
E +I +PLTPGMNAI++ K+ + + VLT R+ GK A ++ +C+D+ E
Sbjct: 142 EIDIHIPLTPGMNAILQCKITNSNIFRDVVLTGKRFGGKEAEKLQLVDKSCTDI---LEK 198

Query: 302 AIKKASELASRNYDRGVYRALKEEVFKWEARELLHGSL 189
+++ A L ++ DR + +LKEE++K ++ELL ++
Sbjct: 199 SVELAELLHTKGKDRITFGSLKEEMYKNASKELLEKTI 236


>tr|A0ACZ7|A0ACZ7_STRAM Putative enoyl-CoA hydratase/isomerase
family protein OS=Streptomyces ambofaciens ATCC 23877
GN=SAMR0642 PE=4 SV=1
Length = 223

Score = 59.7 bits (143), Expect = 8e-08
Identities = 33/73 (45%), Positives = 47/73 (64%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E +I +P TPGM A+I+S+L T HEA+LTA RY G AA AG++ A ++ D+ R TA
Sbjct: 127 EADINIPFTPGMAALIQSRLAPRTAHEAMLTARRYGGTDAAAAGVVDRAVTE-DAVRSTA 185

Query: 299 IKKASELASRNYD 261
I+ A ++ D
Sbjct: 186 IEIAQAQVNKAGD 198


>tr|Q47PN9|Q47PN9_THEFY Putative enoyl-CoA hydratase/isomerase
family protein OS=Thermobifida fusca (strain YX)
GN=Tfu_1544 PE=4 SV=1
Length = 223

Score = 57.4 bits (137), Expect = 4e-07
Identities = 30/65 (46%), Positives = 44/65 (67%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E +IK+P TPGM+A+I+++L T HEA++TA RY G A AGI+ A ++ D R TA
Sbjct: 127 EADIKIPFTPGMSALIQARLSPQTAHEAMVTARRYGGHDAFAAGIVDSAVAE-DEVRATA 185

Query: 299 IKKAS 285
+ A+
Sbjct: 186 LDIAA 190


>tr|B6KFN8|B6KFN8_TOXGO Enoyl-CoA hydratase/isomerase family
protein, putative OS=Toxoplasma gondii ME49
GN=TGME49_042390 PE=4 SV=1
Length = 250

Score = 52.8 bits (125), Expect = 9e-06
Identities = 31/93 (33%), Positives = 49/93 (52%)
Frame = -1

Query: 470 IKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETAIKK 291
I LPLTPGM A+I+SK+ + +L R+ G A A I+ +TA +
Sbjct: 137 IGLPLTPGMCAVIQSKIDRSLWTSTMLRGQRWTGTEALAARIVDNVAVGDAEVLKTARRL 196

Query: 290 ASELASRNYDRGVYRALKEEVFKWEARELLHGS 192
A+ +A+ +R VY ++K E++ E R L G+
Sbjct: 197 AASVAAFGENRVVYGSIKREIYARELRALDEGN 229


>tr|A0Z9E4|A0Z9E4_9GAMM Putative enoyl-CoA hydratase/isomerase
family protein OS=marine gamma proteobacterium HTCC2080
GN=MGP2080_15989 PE=3 SV=1
Length = 220

Score = 50.8 bits (120), Expect = 4e-05
Identities = 33/95 (34%), Positives = 49/95 (51%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E ++ +P+ P M I+++KLP T +AVLT RY A VAGI G S + A
Sbjct: 129 EVDVGVPIPPAMMGILRAKLPASTTRDAVLTGKRYAADEAMVAGIADGQAS-AEELLTQA 187

Query: 299 IKKASELASRNYDRGVYRALKEEVFKWEARELLHG 195
A EL ++ + G++ LK +F A L+ G
Sbjct: 188 KAMARELGTK--EPGIFSTLKTTLFGPTADALVAG 220


>tr|A9NYU1|A9NYU1_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 235

Score = 50.4 bits (119), Expect = 5e-05
Identities = 30/99 (30%), Positives = 49/99 (49%)
Frame = -1

Query: 500 RQRVYNVE*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDV 321
R +Y E +I + L + A+I+SKL T + VL A ++ + A GI+ +D
Sbjct: 122 RSVLYMSELDIGMKLPRSLMAVIRSKLLPGTLRDVVLGARKFSAQMALEGGIVDSVYADS 181

Query: 320 DSTRETAIKKASELASRNYDRGVYRALKEEVFKWEAREL 204
T E A+ +A +LA R + + +Y L+ F EL
Sbjct: 182 PQTLEAAVSEAQKLAGRGWKKEIYSELRLGAFPGVLEEL 220


>tr|A8L469|A8L469_FRASN Enoyl-CoA hydratase/isomerase OS=Frankia sp.
(strain EAN1pec) GN=Franean1_2452 PE=4 SV=1
Length = 223

Score = 50.1 bits (118), Expect = 6e-05
Identities = 27/70 (38%), Positives = 42/70 (60%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E +I +P TP M+A+I+++L T HEA+ TA RY G+ A A I+ A ++ + R T
Sbjct: 127 EADIDIPFTPAMSALIQARLSPQTAHEAMTTARRYGGRDACAAAIVDRAVAE-EEVRGTG 185

Query: 299 IKKASELASR 270
++ A A R
Sbjct: 186 VEIARAQAGR 195


>tr|A8KYK2|A8KYK2_FRASN Enoyl-CoA hydratase/isomerase OS=Frankia sp.
(strain EAN1pec) GN=Franean1_0639 PE=4 SV=1
Length = 225

Score = 48.9 bits (115), Expect = 1e-04
Identities = 26/70 (37%), Positives = 40/70 (57%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E ++ +P + G+ +I++++PV T HEA+ T RY G A AGII G +D + TA
Sbjct: 127 EVDVDIPFSIGITELIRARMPVRTAHEAMTTGRRYGGDDALAAGIIDGV-ADQEKVLATA 185

Query: 299 IKKASELASR 270
I A L +
Sbjct: 186 IAMARPLTGK 195


>tr|B1MBC5|B1MBC5_MYCA9 Putative enoyl-coa hydratase/isomerase
family OS=Mycobacterium abscessus (strain ATCC 19977 /
DSM 44196) GN=MAB_2465c PE=4 SV=1
Length = 216

Score = 46.6 bits (109), Expect = 7e-04
Identities = 31/92 (33%), Positives = 45/92 (48%)
Frame = -1

Query: 479 E*EIKLPLTPGMNAIIKSKLPVPTYHEAVLTAHRYVGKTAAVAGIIHGACSDVDSTRETA 300
E +I +P TPGM ++I++K+ T A+ T HRY G A AG+ +C+ TA
Sbjct: 126 EVDIHIPFTPGMASLIQAKVGPQTAVTAMTTGHRYGGAAAVAAGLA-DSCAAEHEVLSTA 184

Query: 299 IKKASELASRNYDRGVYRALKEEVFKWEAREL 204
I L + D G A+K +F A L
Sbjct: 185 IDLMKPLTGK--DSGTLSAIKATMFAATATAL 214