BP916058
Clone id YMU001_000082_E10
Library
Length 542
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000082_E10.
Accession
Tissue type prothallium
Developmental stage -
Contig ID
Sequence
AATGAAGGCATGTATAGCTAACCTAAGCAGTGTCTTTGCAATAACATTCACGGTCAACAT
ACTCTGAAGGCTCCGACTAAAATTTACGACACCTAACAAACATGTGCCATAAAGAAAACC
TCAACACTTCCAACTCTTCCTGCATCTATGGATTGCCACCTAATGGAGATTTCTGAAATC
AAAAAGCTTCAACATCACTGCAAGAGATGCTTGACACGATTCAAAAAAGGTGCAAGCCCT
TACTTTGACAAAGTTTCTTTAGTGAAAAGTAGCTGCAGAGGCCATTTGATGCAATATTCC
AATGCTATGGACTCCCAGCCATCGTTCAGTTTATTTGAAATATCTCCATGGATGGTCAGA
AGGCGTATGTTCCCTTCAGCACTAGACCTTGGCCTCAAAATCTCTTTGTGTGAGGCTCCC
AAGACGGAAACCCTGGAAACACGAAAGTCTACTGGTCTGCATGCTGCTCGAAGCTGACTT
TGTCTGCTGGTCTGCATGCTCCTTGAGGCCGACTTTGTCGTGGTCACAACACTAAGGCTG
CG
■■Homology search results ■■ -
sp_hit_id Q9M350
Definition sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog OS=Arabidopsis thaliana
Align length 58
Score (bit) 48.5
E-value 2.0e-05
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP916058|Adiantum capillus-veneris mRNA, clone:
YMU001_000082_E10.
(542 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog O... 49 2e-05
sp|Q9SC88|GCP4_MEDTR Gamma-tubulin complex component 4 homolog O... 47 8e-05
sp|Q9D4F8|GCP4_MOUSE Gamma-tubulin complex component 4 OS=Mus mu... 35 0.31
sp|Q9UGJ1|GCP4_HUMAN Gamma-tubulin complex component 4 OS=Homo s... 34 0.40
sp|P40114|TOP1_BACAN DNA topoisomerase 1 OS=Bacillus anthracis G... 32 1.5
sp|Q5R5J6|GCP2_PONAB Gamma-tubulin complex component 2 OS=Pongo ... 32 2.0
sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 OS=Mus mu... 32 2.0
sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 OS=Homo s... 32 2.0
sp|Q06679|UTP4_YEAST U3 small nucleolar RNA-associated protein 4... 31 3.4
sp|Q9H489|TSYL3_HUMAN Testis-specific Y-encoded-like protein 3 O... 31 3.4
sp|Q8T295|PRP8_DICDI Pre-mRNA-processing-splicing factor 8 homol... 31 3.4
sp|P34477|UBC7_CAEEL Probable ubiquitin-conjugating enzyme E2 7 ... 30 5.8
sp|Q8CP47|6PGD_STAES 6-phosphogluconate dehydrogenase, decarboxy... 30 9.9
sp|Q5HP42|6PGD_STAEQ 6-phosphogluconate dehydrogenase, decarboxy... 30 9.9

>sp|Q9M350|GCP4_ARATH Gamma-tubulin complex component 4 homolog
OS=Arabidopsis thaliana GN=At3g53760 PE=2 SV=2
Length = 745

Score = 48.5 bits (114), Expect = 2e-05
Identities = 24/58 (41%), Positives = 35/58 (60%)
Frame = -1

Query: 416 ASHKEILRPRSSAEGNIRLLTIHGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
+S +++R + S G L S+ DGW++IALEY + WP+QL FT+E LSK
Sbjct: 479 SSQADMVRSKVSLTGKANLT------SDTSVDGWDAIALEYSVDWPMQLFFTQEVLSK 530


>sp|Q9SC88|GCP4_MEDTR Gamma-tubulin complex component 4 homolog
OS=Medicago truncatula GN=85P PE=2 SV=1
Length = 739

Score = 46.6 bits (109), Expect = 8e-05
Identities = 23/48 (47%), Positives = 29/48 (60%)
Frame = -1

Query: 386 SSAEGNIRLLTIHGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
S+A I +I S DGW+ IALEY I+WPL L FT+E LS+
Sbjct: 472 SAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSR 519


>sp|Q9D4F8|GCP4_MOUSE Gamma-tubulin complex component 4 OS=Mus
musculus GN=Tubgcp4 PE=2 SV=2
Length = 667

Score = 34.7 bits (78), Expect = 0.31
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -1

Query: 326 NDGWESIALEYCIKWPLQLLFTKETLSK 243
+ GW ++ L Y ++WPL +LFT L K
Sbjct: 447 SSGWAALGLSYKVQWPLHILFTPAVLEK 474


>sp|Q9UGJ1|GCP4_HUMAN Gamma-tubulin complex component 4 OS=Homo
sapiens GN=TUBGCP4 PE=1 SV=1
Length = 667

Score = 34.3 bits (77), Expect = 0.40
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -1

Query: 320 GWESIALEYCIKWPLQLLFTKETLSK 243
GW ++ L Y ++WPL +LFT L K
Sbjct: 449 GWAALGLSYKVQWPLHILFTPAVLEK 474


>sp|P40114|TOP1_BACAN DNA topoisomerase 1 OS=Bacillus anthracis
GN=topX PE=3 SV=2
Length = 870

Score = 32.3 bits (72), Expect = 1.5
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +1

Query: 241 YFDKVSLVKSSCRGHLMQYSNAMDSQPSFSLFEI 342
Y++ + S CRGHL++ N + P + LF++
Sbjct: 21 YYENDHYIVSWCRGHLLELKNPEEMDPKYKLFQL 54


>sp|Q5R5J6|GCP2_PONAB Gamma-tubulin complex component 2 OS=Pongo
abelii GN=TUBGCP2 PE=2 SV=1
Length = 902

Score = 32.0 bits (71), Expect = 2.0
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -1

Query: 350 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
H D + G E+ + +Y +KWPL L+ ++ L++
Sbjct: 602 HADPTELTLSGLEAFSFDYIVKWPLSLIINRKALTR 637


>sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 OS=Mus
musculus GN=Tubgcp2 PE=2 SV=1
Length = 905

Score = 32.0 bits (71), Expect = 2.0
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -1

Query: 350 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
H D + G E+ + +Y +KWPL L+ ++ L++
Sbjct: 602 HADPTELTLSGLEAFSFDYMVKWPLSLIINRKALTR 637


>sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 OS=Homo
sapiens GN=TUBGCP2 PE=1 SV=2
Length = 902

Score = 32.0 bits (71), Expect = 2.0
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -1

Query: 350 HGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
H D + G E+ + +Y +KWPL L+ ++ L++
Sbjct: 602 HADPTELALSGLEAFSFDYIVKWPLSLIINRKALTR 637


>sp|Q06679|UTP4_YEAST U3 small nucleolar RNA-associated protein 4
OS=Saccharomyces cerevisiae GN=UTP4 PE=1 SV=1
Length = 776

Score = 31.2 bits (69), Expect = 3.4
Identities = 22/91 (24%), Positives = 46/91 (50%), Gaps = 10/91 (10%)
Frame = -1

Query: 539 SLSVVTTTKSASRSMQ--------TSRQSQLRAACRPVDFRVSRVSVLGASHKEILRPRS 384
S S+++ K SRS++ TS++ + CR VDF + ++ L SHK + +
Sbjct: 2 SSSLLSVLKEKSRSLKIRNKPVKMTSQERMIVHRCRFVDFTPATITSLAFSHKSNINKLT 61

Query: 383 SAEGNIRLLTIHGDIS--NKLNDGWESIALE 297
++ + + +G+I N N+ ++ + +E
Sbjct: 62 PSDLRLAIGRSNGNIEIWNPRNNWFQEMVIE 92


>sp|Q9H489|TSYL3_HUMAN Testis-specific Y-encoded-like protein 3
OS=Homo sapiens GN=TSPYL3 PE=2 SV=1
Length = 355

Score = 31.2 bits (69), Expect = 3.4
Identities = 16/45 (35%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Frame = +1

Query: 148 MDCHLMEISEIKKLQHHCKRCLTRFKKGASPYFDKVSLVKS-SCR 279
M C+LM + E+++L+H C +F+ ++PYF +VK CR
Sbjct: 217 MLCYLMNL-EVRELRHSRTGCKFKFRFWSNPYFQNKVIVKEYECR 260


tr_hit_id A9NW25
Definition tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 72
Score (bit) 59.3
E-value 1.0e-07
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP916058|Adiantum capillus-veneris mRNA, clone:
YMU001_000082_E10.
(542 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea... 59 1e-07
tr|A9RXH9|A9RXH9_PHYPA Gamma tubulin ring complex protein 4 OS=P... 49 1e-04
tr|Q60F24|Q60F24_ORYSJ Os05g0154500 protein OS=Oryza sativa subs... 49 2e-04
tr|B8AY65|B8AY65_ORYSI Putative uncharacterized protein OS=Oryza... 49 2e-04
tr|A3B039|A3B039_ORYSJ Putative uncharacterized protein OS=Oryza... 49 2e-04
tr|A7Q0E6|A7Q0E6_VITVI Chromosome chr7 scaffold_42, whole genome... 46 0.001
tr|A5BKC3|A5BKC3_VITVI Putative uncharacterized protein OS=Vitis... 46 0.001
tr|Q642S3|Q642S3_XENLA MGC81229 protein OS=Xenopus laevis GN=tub... 37 0.51
tr|B3RSJ9|B3RSJ9_TRIAD Putative uncharacterized protein OS=Trich... 37 0.88
tr|A7GME9|A7GME9_BACCN DNA topoisomerase I OS=Bacillus cereus su... 36 1.1
tr|Q05AR5|Q05AR5_XENTR Tubulin, gamma complex associated protein... 36 1.5
tr|Q8BYN2|Q8BYN2_MOUSE Putative uncharacterized protein OS=Mus m... 36 1.5
tr|Q8BWR3|Q8BWR3_MOUSE Putative uncharacterized protein OS=Mus m... 36 1.5
tr|Q8BKL6|Q8BKL6_MOUSE Putative uncharacterized protein OS=Mus m... 36 1.5
tr|B6L8S9|B6L8S9_BRAFL Putative uncharacterized protein OS=Branc... 35 2.0
tr|Q543T6|Q543T6_MOUSE Putative uncharacterized protein OS=Mus m... 35 3.3
tr|Q3U6E9|Q3U6E9_MOUSE Putative uncharacterized protein OS=Mus m... 35 3.3
tr|Q3TSV8|Q3TSV8_MOUSE Putative uncharacterized protein (Fragmen... 35 3.3
tr|A2YWR4|A2YWR4_ORYSI Putative uncharacterized protein OS=Oryza... 35 3.3
tr|Q5NVE4|Q5NVE4_PONAB Putative uncharacterized protein DKFZp459... 34 4.4
tr|B7NZE4|B7NZE4_RABIT Tubulin, gamma complex associated protein... 34 4.4
tr|B2KIP3|B2KIP3_RHIFE Gamma-tubulin complex component 4 (Predic... 34 4.4
tr|B0KWF7|B0KWF7_CALJA Tubulin, gamma complex associated protein... 34 4.4
tr|A9X1B2|A9X1B2_PAPAN Tubulin, gamma complex associated protein... 34 4.4
tr|A0JNH5|A0JNH5_BOVIN Tubulin, gamma complex associated protein... 34 4.4
tr|B6MLL6|B6MLL6_BRAFL Putative uncharacterized protein OS=Branc... 34 4.4
tr|Q9BVR6|Q9BVR6_HUMAN TUBGCP4 protein OS=Homo sapiens GN=TUBGCP... 34 4.4
tr|B3KNK6|B3KNK6_HUMAN cDNA FLJ14797 fis, clone NT2RP4001256, hi... 34 4.4
tr|Q4T698|Q4T698_TETNG Chromosome undetermined SCAF8850, whole g... 34 5.7
tr|B3DIU9|B3DIU9_DANRE Similar to Gamma tubulin ring complex pro... 34 5.7

>tr|A9NW25|A9NW25_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 754

Score = 59.3 bits (142), Expect = 1e-07
Identities = 30/72 (41%), Positives = 48/72 (66%), Gaps = 2/72 (2%)
Frame = -1

Query: 452 VDFRVSRVSVLGASHKEILRPRSSAEGNIRLLT-IHGDISNKLN-DGWESIALEYCIKWP 279
V R+ + +H +I RP+S+ +G++ L+ ++G+ S + DGW+ IALEY + WP
Sbjct: 466 VSLRMPSLGSTSTTHGDISRPKSTRDGSLGSLSNMYGNSSAGPSLDGWDGIALEYSVDWP 525

Query: 278 LQLLFTKETLSK 243
LQLLFT+E L+K
Sbjct: 526 LQLLFTREVLAK 537


>tr|A9RXH9|A9RXH9_PHYPA Gamma tubulin ring complex protein 4
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_206582 PE=4 SV=1
Length = 735

Score = 49.3 bits (116), Expect = 1e-04
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = -1

Query: 323 DGWESIALEYCIKWPLQLLFTKETLSK 243
DGW+ +ALEY I WPLQLLFTK+ LSK
Sbjct: 502 DGWDGLALEYSINWPLQLLFTKDVLSK 528


>tr|Q60F24|Q60F24_ORYSJ Os05g0154500 protein OS=Oryza sativa subsp.
japonica GN=OSJNBa0059G01.11 PE=4 SV=1
Length = 754

Score = 48.5 bits (114), Expect = 2e-04
Identities = 26/71 (36%), Positives = 43/71 (60%), Gaps = 1/71 (1%)
Frame = -1

Query: 452 VDFRVSRVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 276
V R+S + ++ ++ L+ S+ E ++ G +++L DGW+SIALEY + WPL
Sbjct: 469 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 523

Query: 275 QLLFTKETLSK 243
QL FT + +SK
Sbjct: 524 QLFFTPDVVSK 534


>tr|B8AY65|B8AY65_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_18509 PE=4 SV=1
Length = 762

Score = 48.5 bits (114), Expect = 2e-04
Identities = 26/71 (36%), Positives = 43/71 (60%), Gaps = 1/71 (1%)
Frame = -1

Query: 452 VDFRVSRVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 276
V R+S + ++ ++ L+ S+ E ++ G +++L DGW+SIALEY + WPL
Sbjct: 469 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 523

Query: 275 QLLFTKETLSK 243
QL FT + +SK
Sbjct: 524 QLFFTPDVVSK 534


>tr|A3B039|A3B039_ORYSJ Putative uncharacterized protein OS=Oryza
sativa subsp. japonica GN=OsJ_016411 PE=4 SV=1
Length = 700

Score = 48.5 bits (114), Expect = 2e-04
Identities = 26/71 (36%), Positives = 43/71 (60%), Gaps = 1/71 (1%)
Frame = -1

Query: 452 VDFRVSRVSVLGASHKEILRPRSSAEGNIRLLTIHGDISNKLN-DGWESIALEYCIKWPL 276
V R+S + ++ ++ L+ S+ E ++ G +++L DGW+SIALEY + WPL
Sbjct: 407 VSLRMSSFGIKASTSQKDLQKSSTPE-----ISSQGKATSELALDGWDSIALEYSVDWPL 461

Query: 275 QLLFTKETLSK 243
QL FT + +SK
Sbjct: 462 QLFFTPDVVSK 472


>tr|A7Q0E6|A7Q0E6_VITVI Chromosome chr7 scaffold_42, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00028020001
PE=4 SV=1
Length = 743

Score = 45.8 bits (107), Expect = 0.001
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = -1

Query: 323 DGWESIALEYCIKWPLQLLFTKETLSK 243
+GW+ IALEY + WPLQL FT+E LSK
Sbjct: 501 EGWDGIALEYSVDWPLQLFFTQEVLSK 527


>tr|A5BKC3|A5BKC3_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_003625 PE=4 SV=1
Length = 1529

Score = 45.8 bits (107), Expect = 0.001
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = -1

Query: 323 DGWESIALEYCIKWPLQLLFTKETLSK 243
+GW+ IALEY + WPLQL FT+E LSK
Sbjct: 1287 EGWDGIALEYSVDWPLQLFFTQEVLSK 1313


>tr|Q642S3|Q642S3_XENLA MGC81229 protein OS=Xenopus laevis
GN=tubgcp4 PE=2 SV=1
Length = 666

Score = 37.4 bits (85), Expect = 0.51
Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = -1

Query: 419 GASHKEILRPRSSAEGNIRLLTIHGDISNKL--NDGWESIALEYCIKWPLQLLFTKETLS 246
G HK+ +PR EG R D+S + GW ++ L Y ++WPL +LFT L
Sbjct: 422 GKEHKDTSQPR---EGPFR------DMSPREAPTSGWAALGLSYKVQWPLHILFTPAVLE 472

Query: 245 K 243
K
Sbjct: 473 K 473


>tr|B3RSJ9|B3RSJ9_TRIAD Putative uncharacterized protein
OS=Trichoplax adhaerens GN=TRIADDRAFT_23331 PE=4 SV=1
Length = 646

Score = 36.6 bits (83), Expect = 0.88
Identities = 28/81 (34%), Positives = 41/81 (50%), Gaps = 4/81 (4%)
Frame = -1

Query: 422 LGASHKEILRPRSSAEGNIRLLTIHGDISNKLNDGWESIALEYCIKWPLQLLFTKETLSK 243
L H +I++P + NI + D + GW + L+Y ++WPLQL+ TK L K
Sbjct: 400 LSQFHLKIIQPEVEQK-NIPIEQQESD-REEAECGWTCLNLQYNVQWPLQLILTKPILEK 457

Query: 242 *G-LAPFL---NRVKHLLQ*C 192
L FL NR++ LQ C
Sbjct: 458 YNVLFRFLISVNRIQLELQHC 478


>tr|A7GME9|A7GME9_BACCN DNA topoisomerase I OS=Bacillus cereus
subsp. cytotoxis (strain NVH 391-98) GN=Bcer98_0977 PE=4
SV=1
Length = 96

Score = 36.2 bits (82), Expect = 1.1
Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
Frame = +1

Query: 172 SEIKKLQ--HHCKRCLTRFKKGASPYFDKVSLVKSSCRGHLMQYSNAMDSQPSFSLFEI 342
+EI KL HH ++ ++ + PY++ + S CRGHL++ N + P + +F++
Sbjct: 22 NEIMKLPRFHHSQKYIS-----SKPYYENNHYIVSWCRGHLLELKNPEEMDPMYKVFKL 75