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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
BP919899
Clone id YMU001_000130_E02
Library
Length 489
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000130_E02.
Accession
Tissue type prothallium
Developmental stage -
Contig ID -
Sequence 
CTCTGGCGCTACATGTAATTTAACATCCAACCGTGAGGACTTGAGAAATTATAAACCATT
GACTAAATCCTTTGAAGTTCGCTTTGGGAATAATAGCACTCAACAAGCCTTAGGAGTAGG
AACAGCACACATTATGCTATCTGATGGCAATGTAGTGGCTGTTGACAAAGTATACTATGT
TCCCCATATCGCTAAAAATCTGATCTCTGTCAGCCAGATTGCACAAACCGGCACTAAGGT
TGAGTTTGGAGCTAACCATTGTACAATCAAACATCACCTTCCTCGCGGTGGTCGGTATGA
AGTACTAGGCCCCAAAATTGGGAAACTTTACCTTTTGGGACACACAAAACCTTCAGAAGA
ATGCCACAGTACCTCTTTGACCCCTAACCCCGATCTATAGTACGCTACTATGGCATTATC
GTGTAGGGCACCTTAACTTAGCCACAATGCAAACAATGGCCAACCGTAACTTAGTAAATC
ATTATGCAT
■■Homology search results ■■ -
sp_hit_id P10978
Definition sp|P10978|POLX_TOBAC Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum
Align length 85
Score (bit) 32.0
E-value 0.001
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP919899|Adiantum capillus-veneris mRNA, clone:
YMU001_000130_E02.
         (489 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P10978|POLX_TOBAC Retrovirus-related Pol polyprotein from tra...    32   0.001
sp|O05954|MURE_RICPR UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--...    29   9.9  

>sp|P10978|POLX_TOBAC Retrovirus-related Pol polyprotein from
           transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1
          Length = 1328

 Score = 32.0 bits (71), Expect(2) = 0.001
 Identities = 26/85 (30%), Positives = 38/85 (44%)
 Frame = +2

Query: 77  VRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYVPHIAKNLISVSQIAQTGTKVEFGAN 256
           V+ GN S  +  G+G   I  + G  + +  V +VP +  NLIS   + + G +  F AN
Sbjct: 322 VKMGNTSYSKIAGIGDICIKTNVGCTLVLKDVRHVPDLRMNLISGIALDRDGYESYF-AN 380

Query: 257 HCTIKHHLPRGGRYEVLGPKIGKLY 331
               K  L +G      G   G LY
Sbjct: 381 Q---KWRLTKGSLVIAKGVARGTLY 402



 Score = 28.9 bits (63), Expect(2) = 0.001
 Identities = 9/25 (36%), Positives = 19/25 (76%)
 Frame = +3

Query: 408 LWHYRVGHLNLATMQTMANRNLVNH 482
           LWH R+GH++   +Q +A ++L+++
Sbjct: 424 LWHKRMGHMSEKGLQILAKKSLISY 448


>sp|O05954|MURE_RICPR
           UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
           6-diaminopimelate ligase OS=Rickettsia prowazekii
           GN=murE PE=3 SV=1
          Length = 480

 Score = 29.3 bits (64), Expect = 9.9
 Identities = 15/45 (33%), Positives = 22/45 (48%)
 Frame = +2

Query: 86  GNNSTQQALGVGTAHIMLSDGNVVAVDKVYYVPHIAKNLISVSQI 220
           GN+    AL  G   ++  + N  A+DKV YV  + K L    +I
Sbjct: 40  GNDFINDALNNGAVLVITENKNNTAIDKVIYVEDVHKALYEAIEI 84
tr_hit_id Q572I3
Definition tr|Q572I3|Q572I3_PHYIN Putative polyprotein OS=Phytophthora infestans
Align length 124
Score (bit) 59.3
E-value 5.0e-09
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BP919899|Adiantum capillus-veneris mRNA, clone:
YMU001_000130_E02.
         (489 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q572I3|Q572I3_PHYIN Putative polyprotein OS=Phytophthora infe...    59   5e-09
tr|Q94EV3|Q94EV3_MAIZE Fourf gag/pol protein OS=Zea mays GN=Four...    59   6e-09
tr|A5AEN5|A5AEN5_VITVI Putative uncharacterized protein OS=Vitis...    53   1e-08
tr|Q8S476|Q8S476_MAIZE Putative Fourf gag/pol protein OS=Zea may...    57   2e-08
tr|A5APH6|A5APH6_VITVI Putative uncharacterized protein OS=Vitis...    58   2e-08
tr|A5C3I5|A5C3I5_VITVI Putative uncharacterized protein OS=Vitis...    54   3e-08
tr|A5BBW7|A5BBW7_VITVI Putative uncharacterized protein OS=Vitis...    55   8e-08
tr|B8MFW4|B8MFW4_9EURO Putative uncharacterized protein OS=Talar...    54   1e-07
tr|Q01M93|Q01M93_ORYSA OSIGBa0146N20.7 protein OS=Oryza sativa G...    49   1e-07
tr|A5BWL7|A5BWL7_VITVI Putative uncharacterized protein OS=Vitis...    55   1e-07
tr|B8LUX2|B8LUX2_9EURO Putative uncharacterized protein OS=Talar...    54   1e-07
tr|A7R7G1|A7R7G1_VITVI Chromosome undetermined scaffold_1754, wh...    53   1e-07
tr|A5BPR9|A5BPR9_VITVI Putative uncharacterized protein OS=Vitis...    51   2e-07
tr|A5AN26|A5AN26_VITVI Putative uncharacterized protein OS=Vitis...    52   2e-07
tr|Q6BCY1|Q6BCY1_IPOBA Gag-Pol OS=Ipomoea batatas GN=Rtsp-1AA PE...    49   3e-07
tr|A5AM64|A5AM64_VITVI Putative uncharacterized protein OS=Vitis...    54   3e-07
tr|B8MV91|B8MV91_9EURO Putative uncharacterized protein (Fragmen...    53   3e-07
tr|Q9SHR5|Q9SHR5_ARATH F28L22.3 protein OS=Arabidopsis thaliana ...    47   4e-07
tr|O81903|O81903_ARATH Putative transposable element OS=Arabidop...    47   4e-07
tr|A5BA89|A5BA89_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5BIC7|A5BIC7_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5CBM1|A5CBM1_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5BGX3|A5BGX3_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5C6L3|A5C6L3_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5B0C8|A5B0C8_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5BPB3|A5BPB3_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A5AQN2|A5AQN2_VITVI Putative uncharacterized protein OS=Vitis...    53   4e-07
tr|A7QG92|A7QG92_VITVI Chromosome undetermined scaffold_91, whol...    53   4e-07
tr|A5AVU6|A5AVU6_VITVI Putative uncharacterized protein OS=Vitis...    53   5e-07
tr|A5B0V5|A5B0V5_VITVI Putative uncharacterized protein OS=Vitis...    53   5e-07

>tr|Q572I3|Q572I3_PHYIN Putative polyprotein OS=Phytophthora
           infestans GN=PI35.0430c PE=4 SV=1
          Length = 1353

 Score = 59.3 bits (142), Expect(2) = 5e-09
 Identities = 38/124 (30%), Positives = 60/124 (48%), Gaps = 2/124 (1%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           SGAT ++T +R DL  Y+ L     V   +    +  G GT  +   DG  + +  V ++
Sbjct: 252 SGATSHMTPHRNDLFGYEALDSGINVTIADGKKLRVAGTGTVKLTGLDGKRIRMVDVLHI 311

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVL--GPKIGKLYLLGHTKPS 355
           P + + L+SV ++A+ G  V F  + C I       GR   +  G K+GK +LL   +  
Sbjct: 312 PGLDRRLLSVGKLAERGMSVAFQRSSCVI------WGRNAAIASGKKVGKAFLLDCQQ-- 363

Query: 356 EECH 367
           EE H
Sbjct: 364 EEAH 367



 Score = 24.3 bits (51), Expect(2) = 5e-09
 Identities = 8/10 (80%), Positives = 9/10 (90%)
 Frame = +3

Query: 408 LWHYRVGHLN 437
           LWH R+GHLN
Sbjct: 380 LWHARMGHLN 389


>tr|Q94EV3|Q94EV3_MAIZE Fourf gag/pol protein OS=Zea mays GN=Fourf
           gag/pol PE=4 SV=1
          Length = 1279

 Score = 58.5 bits (140), Expect(2) = 6e-09
 Identities = 39/133 (29%), Positives = 65/133 (48%), Gaps = 1/133 (0%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           SGA  ++ ++     +Y+ + +S  +  GN S    LGVGT  +  + G  V +  V +V
Sbjct: 280 SGANIHVCADASMFTSYQ-VGRSGALLMGNGSRAHVLGVGTVILKFTSGKTVPLKSVQHV 338

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYLLG-HTKPSE 358
           P I KNL+S S + + G KV   +N C +  H    G +   G + G L+ L  H   ++
Sbjct: 339 PSIKKNLVSASMLCRDGYKVVLESNKCVVSKH----GTFVGKGYECGGLFRLSLHDVCNK 394

Query: 359 ECHSTSLTPNPDL 397
             +S + +   DL
Sbjct: 395 LVNSVNFSDESDL 407



 Score = 24.6 bits (52), Expect(2) = 6e-09
 Identities = 9/23 (39%), Positives = 13/23 (56%)
 Frame = +3

Query: 408 LWHYRVGHLNLATMQTMANRNLV 476
           LWH R  H +   +  +AN NL+
Sbjct: 407 LWHSRFCHASFGCLMRLANINLI 429


>tr|A5AEN5|A5AEN5_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_011340 PE=4 SV=1
          Length = 1316

 Score = 52.8 bits (125), Expect(2) = 1e-08
 Identities = 32/111 (28%), Positives = 58/111 (52%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           +GAT ++ S+++    ++P+    +V  GN++T +  G G   + ++ G  + +  V YV
Sbjct: 340 TGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLTNVLYV 399

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYL 334
           P I KNL+S S +   G ++ F +N    K  L + G Y      +GK Y+
Sbjct: 400 PEIRKNLVSGSLLNNHGFRLVFXSN----KVVLSKSGMY------VGKGYM 440



 Score = 29.3 bits (64), Expect(2) = 1e-08
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +3

Query: 399 STLLWHYRVGHLNLATMQTMANRN 470
           S+ LWH R+GH+N  T++ + N N
Sbjct: 469 SSNLWHGRLGHVNYDTLRRLINLN 492


>tr|Q8S476|Q8S476_MAIZE Putative Fourf gag/pol protein OS=Zea mays
           GN=Z138B04.13 PE=4 SV=1
          Length = 1292

 Score = 57.0 bits (136), Expect(2) = 2e-08
 Identities = 39/133 (29%), Positives = 63/133 (47%), Gaps = 1/133 (0%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           SGA  ++ ++     +Y+ + +S  +  GN S    LGVGT  +  + G  V +  V +V
Sbjct: 320 SGANIHVCADASMFTSYQ-VGRSGALLMGNGSRAHVLGVGTVILKFTSGKTVPLKSVQHV 378

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYLLG-HTKPSE 358
           P I KNL+S S + + G KV   +N C +  H    G +   G   G L+ L  H   ++
Sbjct: 379 PSIKKNLVSASMLCRDGYKVVLESNKCVVSKH----GTFVGKGYDCGGLFRLSLHDVCNK 434

Query: 359 ECHSTSLTPNPDL 397
             +S   +   DL
Sbjct: 435 LVNSVHFSDESDL 447



 Score = 24.6 bits (52), Expect(2) = 2e-08
 Identities = 9/23 (39%), Positives = 13/23 (56%)
 Frame = +3

Query: 408 LWHYRVGHLNLATMQTMANRNLV 476
           LWH R  H +   +  +AN NL+
Sbjct: 447 LWHSRFCHASFGCLMRLANINLI 469


>tr|A5APH6|A5APH6_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_018181 PE=4 SV=1
          Length = 1069

 Score = 57.8 bits (138), Expect(2) = 2e-08
 Identities = 37/111 (33%), Positives = 57/111 (51%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           SGA  + T +RE ++NY       +V   NNST   +G+G   I L +G+V  ++KV ++
Sbjct: 290 SGALFHTTPHREIIQNYAAGDFG-KVYLANNSTLDVVGLGDVQISLXNGSVWLLEKVRHI 348

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYL 334
           P + +NLISV Q+   G  + F      +     +G R    G K G LY+
Sbjct: 349 PDLRRNLISVGQLDDEGHAILFVGGTWKV----TKGARVLARGKKTGTLYM 395



 Score = 23.9 bits (50), Expect(2) = 2e-08
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = +3

Query: 402 TLLWHYRVGHLNLATMQTMANR 467
           T LWH R+GH++   M+ + ++
Sbjct: 412 TSLWHRRLGHMSEKGMKMLLSK 433


>tr|A5C3I5|A5C3I5_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_041386 PE=4 SV=1
          Length = 1132

 Score = 53.5 bits (127), Expect(2) = 3e-08
 Identities = 38/99 (38%), Positives = 51/99 (51%), Gaps = 2/99 (2%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSF--EVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVY 175
           SG T ++T    D R +K L K+   +VR GN +     G GT  I    G +  +  V 
Sbjct: 155 SGCTNHMTY---DQRLFKELNKTITSKVRIGNGAYLAVKGKGTVAIEGHTG-LKLISNVL 210

Query: 176 YVPHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGG 292
           YVP I +NL+S+SQ+ + G KV F  NHC I     R G
Sbjct: 211 YVPEINQNLLSISQLLEKGYKVLFEDNHCMIADAQGREG 249



 Score = 27.3 bits (59), Expect(2) = 3e-08
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +3

Query: 399 STLLWHYRVGHLNLATMQTMANRNL 473
           +T+LWH R+GH +  T+  M   +L
Sbjct: 277 NTMLWHKRLGHFHHTTLLFMKKNDL 301


>tr|A5BBW7|A5BBW7_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_021529 PE=4 SV=1
          Length = 1033

 Score = 55.1 bits (131), Expect(2) = 8e-08
 Identities = 35/112 (31%), Positives = 58/112 (51%), Gaps = 1/112 (0%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSF-EVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYY 178
           SGA+ + T +RE ++NY  +   F +V     S    +G+G   I+L +G++  ++KV Y
Sbjct: 290 SGASFHTTPHREIMQNY--VANDFGKVYLVYGSAMDVVGLGDVRILLPNGSIWLLEKVRY 347

Query: 179 VPHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYL 334
           +P + +NLISV Q+   G  + F      +     +G R    G K G LY+
Sbjct: 348 IPDLMRNLISVGQLDDEGHAILFVGGTWKV----TKGARILARGKKTGTLYM 395



 Score = 24.3 bits (51), Expect(2) = 8e-08
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = +3

Query: 402 TLLWHYRVGHLNLATMQTMANR 467
           T LWH R+GH++   M+ + ++
Sbjct: 412 TSLWHRRLGHMSEKEMKMLLSK 433


>tr|B8MFW4|B8MFW4_9EURO Putative uncharacterized protein
           OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_009530 PE=4
           SV=1
          Length = 1395

 Score = 53.5 bits (127), Expect(2) = 1e-07
 Identities = 37/120 (30%), Positives = 65/120 (54%), Gaps = 2/120 (1%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           SGAT +  +++ DL++YK   +  E+  G+N+T  A G GT H+ +  G  + V  V++V
Sbjct: 349 SGATQHFCNSKLDLKDYKHFLEPREIYLGDNTTIYAEGSGTQHLQV--GPYILVLNVWFV 406

Query: 182 PHIAKNLISVSQIAQTG--TKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYLLGHTKPS 355
           P +A+NL+S+  + + G  T +E G  +   +        +++   K G LY + H  PS
Sbjct: 407 PKLAENLLSLQLLDRAGYSTLIENGIVYIR-QQGDSNSAWFQLANSKHGDLYRM-HISPS 464



 Score = 25.4 bits (54), Expect(2) = 1e-07
 Identities = 11/24 (45%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
 Frame = +3

Query: 396 YSTL-LWHYRVGHLNLATMQTMAN 464
           +STL LWH R+GH N  ++  + N
Sbjct: 478 FSTLRLWHNRLGHRNFRSVGDLMN 501


>tr|Q01M93|Q01M93_ORYSA OSIGBa0146N20.7 protein OS=Oryza sativa
           GN=OSIGBa0146N20.7 PE=4 SV=1
          Length = 1335

 Score = 48.9 bits (115), Expect(2) = 1e-07
 Identities = 35/129 (27%), Positives = 65/129 (50%), Gaps = 2/129 (1%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSFEVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYYV 181
           S  + ++ + R    +YKP+ K   VR G+++    +G+G+  I   DG    +  V Y+
Sbjct: 296 SACSFHICTKRNWFSSYKPVQKGDVVRMGDDNPCAIVGIGSVQIKTDDGMTRTLKNVRYI 355

Query: 182 PHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGP-KIGKLYLL-GHTKPS 355
           P +++NLIS+S +   G K  +  +   +K  + +G    + G     KLY+L G T P 
Sbjct: 356 PGMSRNLISLSTLDAEGYK--YSGSDGVLK--VSKGSLVCLKGDLNSAKLYVLRGCTLPG 411

Query: 356 EECHSTSLT 382
            +  + ++T
Sbjct: 412 SDSAAAAVT 420



 Score = 30.0 bits (66), Expect(2) = 1e-07
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +3

Query: 402 TLLWHYRVGHLNLATMQTMANRNLVNHY 485
           T LWH R+GH++   M  +  RNL+  Y
Sbjct: 427 TNLWHMRLGHMSHLGMTELMKRNLLKGY 454


>tr|A5BWL7|A5BWL7_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_038864 PE=4 SV=1
          Length = 1172

 Score = 54.7 bits (130), Expect(2) = 1e-07
 Identities = 35/112 (31%), Positives = 59/112 (52%), Gaps = 1/112 (0%)
 Frame = +2

Query: 2   SGATCNLTSNREDLRNYKPLTKSF-EVRFGNNSTQQALGVGTAHIMLSDGNVVAVDKVYY 178
           SGA+ + T +RE ++NY  +   F +V   + ST   +G+G   I L +G++  ++KV +
Sbjct: 222 SGASFHTTPHREIIQNY--VAGDFGKVYLADGSTLDVVGLGDVRITLPNGSIWLLEKVRH 279

Query: 179 VPHIAKNLISVSQIAQTGTKVEFGANHCTIKHHLPRGGRYEVLGPKIGKLYL 334
           +P + +NLISV Q+   G  + F      +     +G R    G K G LY+
Sbjct: 280 IPDLRRNLISVGQLDDEGHAILFVGGTWKV----TKGARVLARGKKTGTLYM 327



 Score = 24.3 bits (51), Expect(2) = 1e-07
 Identities = 8/23 (34%), Positives = 16/23 (69%)
 Frame = +3

Query: 399 STLLWHYRVGHLNLATMQTMANR 467
           +T LWH R+GH++   M+ + ++
Sbjct: 343 NTSLWHRRLGHMSEKGMKMLLSK 365