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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK947972
Clone id TST38A01NGRL0002_B02
Library
Length 616
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0002_B02. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GTTTAAAGGTGCAGTAAAACTTAATATGCCACAGGCTAGAGAAAATTTTGATCTGTTCAA
GGGAGCTGCTGGAAAAGCTTACCTGCGGAGTGGTTTCTCCACGAAACGACCTGATGATCC
ACATCTTAGCAACGTAAATTATCAGAGGCCTGGTGCACGTACGACAGAGGACACAACAGC
GCAAAACTTTACACCGAGTGGAATAGGTGCATCACGTCTAAAAAAGTACAATCTGGTCAC
TTCACAGACCCCACATTCTGGGGAGGTGGGTCAAGCCAGTAGATCAGATGTCCCTATCAC
ATCATTGCATGAAGATGCTGCAAGGGTCCGTATTCGTGAAAATAGTCTTGGAGACTATTG
GAATAGCTGGGTCAGACTGCTCGAGGAGCAGGATCATGGTGCTAGGATAAAGTGCAGGCA
GTTGATTGCACCGTCCGACTATCTTACAAACTTTGTAGCAGACTGTCTAGAGGTGCCCCA
AGAGCAGTATCTGTATTCCAACGAAGGAGCATCAGGTAGAGTGGATTCACAACCTCATAA
TAAAAATTCTGGCTTCCACAGAAGGTTTCCAGATGATGGCAGTTCTCCTTCCGGGATGCG
TTCAGTTAATCTCTCT
■■Homology search results ■■ -
sp_hit_id Q48E77
Definition sp|Q48E77|IF2_PSE14 Translation initiation factor IF-2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
Align length 57
Score (bit) 32.3
E-value 2.0
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK947972|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0002_B02, 5'
         (616 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q48E77|IF2_PSE14 Translation initiation factor IF-2 OS=Pseudo...    32   2.0  
sp|Q28GQ3|OCAD1_XENTR OCIA domain-containing protein 1 OS=Xenopu...    32   3.4  
sp|Q9BXI6|TB10A_HUMAN TBC1 domain family member 10A OS=Homo sapi...    31   4.5  
sp|Q3KI84|IF2_PSEPF Translation initiation factor IF-2 OS=Pseudo...    31   4.5  
sp|Q4KIF6|IF2_PSEF5 Translation initiation factor IF-2 OS=Pseudo...    31   4.5  
sp|O96133|YB145_PLAF7 Uncharacterized protein PFB0145c OS=Plasmo...    30   7.6  
sp|O89084|PDE4A_MOUSE cAMP-specific 3',5'-cyclic phosphodiestera...    30   7.6  
sp|A0LIN6|PDXA_SYNFM 4-hydroxythreonine-4-phosphate dehydrogenas...    30   9.9  

>sp|Q48E77|IF2_PSE14 Translation initiation factor IF-2
           OS=Pseudomonas syringae pv. phaseolicola (strain 1448A /
           Race 6) GN=infB PE=3 SV=1
          Length = 843

 Score = 32.3 bits (72), Expect = 2.0
 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
 Frame = +2

Query: 92  GFSTKRPDDPHLSNVNYQRPGART---TEDTTAQNFTPSGIGASRLKKYNLVTSQTP 253
           G   +R + PH ++V  + P  R    T D  + +F   G G SRLKK N    Q+P
Sbjct: 200 GGDGERKNAPHRASVKEKAPAPRVAPRTTDEESDSFRRGGRGKSRLKKRNAHGFQSP 256


>sp|Q28GQ3|OCAD1_XENTR OCIA domain-containing protein 1 OS=Xenopus
           tropicalis GN=ociad1 PE=2 SV=1
          Length = 254

 Score = 31.6 bits (70), Expect = 3.4
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +2

Query: 227 YNLVTSQTPHSGEVGQASRSDVPITSLHEDAARVRIRENSLGDYW 361
           Y+ + S+   + E+    R+D P+ S  + AAR  ++ N  GD W
Sbjct: 208 YDELRSRNRETYEMAVTQRADAPVRSSLDRAARKDVKTNKYGDVW 252


>sp|Q9BXI6|TB10A_HUMAN TBC1 domain family member 10A OS=Homo sapiens
           GN=TBC1D10A PE=1 SV=1
          Length = 508

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 2/100 (2%)
 Frame = +2

Query: 143 QRPGARTTEDTTA--QNFTPSGIGASRLKKYNLVTSQTPHSGEVGQASRSDVPITSLHED 316
           Q P A TT++ ++   +   +G    R+ K+  +       G +      +VP+  L + 
Sbjct: 28  QGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAL-----EEVPLEVLRQ- 81

Query: 317 AARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPS 436
                 RE+   D  N+W + + ++    R++C++ I PS
Sbjct: 82  ------RESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPS 115


>sp|Q3KI84|IF2_PSEPF Translation initiation factor IF-2
           OS=Pseudomonas fluorescens (strain Pf0-1) GN=infB PE=3
           SV=1
          Length = 837

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
 Frame = +2

Query: 86  RSGFSTKRPDDPHLSNVNYQRPGART---TEDTTAQNFTPSGIGASRLKKYNLVTSQTP 253
           R G   +R + PH ++V  + P  R    T D  +  F   G G ++LKK N    Q+P
Sbjct: 192 RRGGDGERKNAPHRASVKEKAPAPRVAPRTTDEESDGFRRGGRGKAKLKKRNAHGFQSP 250


>sp|Q4KIF6|IF2_PSEF5 Translation initiation factor IF-2
           OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
           GN=infB PE=3 SV=1
          Length = 838

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
 Frame = +2

Query: 86  RSGFSTKRPDDPHLSNVNYQRPGART---TEDTTAQNFTPSGIGASRLKKYNLVTSQTP 253
           R G   +R + PH ++V  + P  R    T D  +  F   G G ++LKK N    Q+P
Sbjct: 193 RRGGDGERKNAPHRASVKEKAPAPRVAPRTTDEESDGFRRGGRGKAKLKKRNAHGFQSP 251


>sp|O96133|YB145_PLAF7 Uncharacterized protein PFB0145c OS=Plasmodium
            falciparum (isolate 3D7) GN=PFB0145c PE=2 SV=1
          Length = 1979

 Score = 30.4 bits (67), Expect = 7.6
 Identities = 16/75 (21%), Positives = 34/75 (45%)
 Frame = +2

Query: 335  RENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQEQYLYSNEGAS 514
            R+N   D +  +++L++       I+C   I   D +            E+Y+ +N+G  
Sbjct: 906  RDNKYSDLYEKYIKLIKSICMIINIECCDDIENEDIIRRI---------EEYINNNKGLK 956

Query: 515  GRVDSQPHNKNSGFH 559
              V+ + H ++S F+
Sbjct: 957  KEVEEKEHKRHSSFN 971


>sp|O89084|PDE4A_MOUSE cAMP-specific 3',5'-cyclic phosphodiesterase
           4A OS=Mus musculus GN=Pde4a PE=2 SV=2
          Length = 844

 Score = 30.4 bits (67), Expect = 7.6
 Identities = 21/68 (30%), Positives = 30/68 (44%)
 Frame = +2

Query: 146 RPGARTTEDTTAQNFTPSGIGASRLKKYNLVTSQTPHSGEVGQASRSDVPITSLHEDAAR 325
           RPG RTT  +   +F  +G G    ++       TP  G     S++  P   LH  AA 
Sbjct: 76  RPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQAS-PGLMLHAGAAT 134

Query: 326 VRIRENSL 349
            + RE+ L
Sbjct: 135 SQRRESFL 142


>sp|A0LIN6|PDXA_SYNFM 4-hydroxythreonine-4-phosphate dehydrogenase
           OS=Syntrophobacter fumaroxidans (strain DSM 10017 /
           MPOB) GN=pdxA PE=3 SV=1
          Length = 345

 Score = 30.0 bits (66), Expect = 9.9
 Identities = 21/62 (33%), Positives = 29/62 (46%)
 Frame = +2

Query: 113 DDPHLSNVNYQRPGARTTEDTTAQNFTPSGIGASRLKKYNLVTSQTPHSGEVGQASRSDV 292
           D P L NV   R   R T +   ++F   G+ A RL     V    PH+GE G+  R ++
Sbjct: 178 DVPALLNVEMLREVIRITAEALIRDF---GLQAPRLA----VAGLNPHAGEEGRFGREEI 230

Query: 293 PI 298
            I
Sbjct: 231 EI 232
tr_hit_id B1Z3Y5
Definition tr|B1Z3Y5|B1Z3Y5_BURA4 Short-chain dehydrogenase/reductase SDR OS=Burkholderia ambifaria (strain MC40-6)
Align length 71
Score (bit) 38.5
E-value 0.32
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK947972|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0002_B02, 5'
         (616 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B1Z3Y5|B1Z3Y5_BURA4 Short-chain dehydrogenase/reductase SDR O...    39   0.32 
tr|A4JSV6|A4JSV6_BURVG Short-chain dehydrogenase/reductase SDR O...    39   0.32 
tr|Q39MU4|Q39MU4_BURS3 Short-chain dehydrogenase/reductase SDR O...    38   0.42 
tr|Q1BJ47|Q1BJ47_BURCA Short-chain dehydrogenase/reductase SDR O...    38   0.42 
tr|B1KB78|B1KB78_BURCC Short-chain dehydrogenase/reductase SDR O...    38   0.42 
tr|A0KE88|A0KE88_BURCH Short-chain dehydrogenase/reductase SDR O...    38   0.42 
tr|B1FIM9|B1FIM9_9BURK Short-chain dehydrogenase/reductase SDR O...    38   0.54 
tr|Q2NC63|Q2NC63_ERYLH Alpha-glucosidase OS=Erythrobacter litora...    37   1.2  
tr|Q0B1X1|Q0B1X1_BURCM Short-chain dehydrogenase/reductase SDR O...    37   1.2  
tr|Q584T7|Q584T7_9TRYP Putative uncharacterized protein OS=Trypa...    36   1.6  
tr|Q1GWR3|Q1GWR3_SPHAL Alpha-glucosidase OS=Sphingopyxis alasken...    36   2.1  
tr|Q1NHK1|Q1NHK1_9SPHN Alpha-glucosidase OS=Sphingomonas sp. SKA...    35   3.5  
tr|Q5CN73|Q5CN73_CRYHO Putative uncharacterized protein OS=Crypt...    34   6.0  
tr|B0N5Q7|B0N5Q7_9FIRM Putative uncharacterized protein OS=Clost...    34   7.8  
tr|Q5AXW1|Q5AXW1_EMENI Putative uncharacterized protein OS=Emeri...    34   7.8  

>tr|B1Z3Y5|B1Z3Y5_BURA4 Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia ambifaria (strain MC40-6)
           GN=BamMC406_6012 PE=3 SV=1
          Length = 262

 Score = 38.5 bits (88), Expect = 0.32
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAADRIRVNSVSPGW-TWSRVMDELTHGDRAKTNRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|A4JSV6|A4JSV6_BURVG Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia vietnamiensis (strain G4 / LMG 22486)
           GN=Bcep1808_6462 PE=3 SV=1
          Length = 262

 Score = 38.5 bits (88), Expect = 0.32
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAADRIRVNSVSPGW-TWSRVMDEMTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|Q39MU4|Q39MU4_BURS3 Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia sp. (strain 383) GN=Bcep18194_C7178 PE=3
           SV=1
          Length = 262

 Score = 38.1 bits (87), Expect = 0.42
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAPDRIRVNSVSPGW-TWSRVMDEMTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|Q1BJ47|Q1BJ47_BURCA Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia cenocepacia (strain AU 1054)
           GN=Bcen_5486 PE=3 SV=1
          Length = 262

 Score = 38.1 bits (87), Expect = 0.42
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAPDRIRVNSVSPGW-TWSRVMDEMTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|B1KB78|B1KB78_BURCC Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia cenocepacia (strain MC0-3)
           GN=Bcenmc03_6360 PE=4 SV=1
          Length = 262

 Score = 38.1 bits (87), Expect = 0.42
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAPDRIRVNSVSPGW-TWSRVMDEMTHGDRAKTDRVAAPF-HLLRRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|A0KE88|A0KE88_BURCH Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia cenocepacia (strain HI2424)
           GN=Bcen2424_6772 PE=3 SV=1
          Length = 262

 Score = 38.1 bits (87), Expect = 0.42
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAPDRIRVNSVSPGW-TWSRVMDEMTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|B1FIM9|B1FIM9_9BURK Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia ambifaria IOP40-10
           GN=BamIOP4010DRAFT_3889 PE=3 SV=1
          Length = 262

 Score = 37.7 bits (86), Expect = 0.54
 Identities = 26/71 (36%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D A  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLAADRIRVNSVSPGW-TWSRVMDELTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|Q2NC63|Q2NC63_ERYLH Alpha-glucosidase OS=Erythrobacter litoralis
           (strain HTCC2594) GN=ELI_03180 PE=4 SV=1
          Length = 681

 Score = 36.6 bits (83), Expect = 1.2
 Identities = 31/122 (25%), Positives = 50/122 (40%)
 Frame = +2

Query: 164 TEDTTAQNFTPSGIGASRLKKYNLVTSQTPHSGEVGQASRSDVPITSLHEDAARVRIREN 343
           TE   A   T   I A    +Y  +  +TP +G     S +  P+T   ED   +   E 
Sbjct: 151 TEFVIAGEGTAWWINAGAFNRYEYIYQETPITG----VSTAHTPMTVRLEDGTHLSFHEA 206

Query: 344 SLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQEQYLYSNEGASGRV 523
           +L DY   W++ +E Q      + R  +APS      V         + +  ++ A+G V
Sbjct: 207 ALVDYSGMWLKRIEGQ------RFRATLAPSSRGAKVVRKGAFTTPWRTIRISDDAAGLV 260

Query: 524 DS 529
           +S
Sbjct: 261 ES 262


>tr|Q0B1X1|Q0B1X1_BURCM Short-chain dehydrogenase/reductase SDR
           OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)
           GN=Bamb_6308 PE=3 SV=1
          Length = 262

 Score = 36.6 bits (83), Expect = 1.2
 Identities = 25/71 (35%), Positives = 37/71 (52%)
 Frame = +2

Query: 302 SLHEDAARVRIRENSLGDYWNSWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVPQ 481
           S+  D +  RIR NS+   W +W R+++E  HG R K  ++ AP  +L   V D  EV Q
Sbjct: 164 SMAMDLSADRIRVNSVSPGW-TWSRVMDELTHGDRAKTDRVAAPF-HLLGRVGDPSEVAQ 221

Query: 482 EQYLYSNEGAS 514
                 ++ AS
Sbjct: 222 VVTFLCSDAAS 232


>tr|Q584T7|Q584T7_9TRYP Putative uncharacterized protein
           OS=Trypanosoma brucei GN=Tb927.6.2190 PE=4 SV=1
          Length = 287

 Score = 36.2 bits (82), Expect = 1.6
 Identities = 17/85 (20%), Positives = 45/85 (52%), Gaps = 2/85 (2%)
 Frame = +2

Query: 305 LHEDAARVRIRENSLGDYWN--SWVRLLEEQDHGARIKCRQLIAPSDYLTNFVADCLEVP 478
           +  + ++ +  +  L + W+  +  ++ EE++ G R + R +++P   L +  A+ ++  
Sbjct: 82  IRAETSKQKHLQRQLQEVWDRVAMAKVEEEKEEGVRRRSRYVVSPPAILHHSTAELVQEN 141

Query: 479 QEQYLYSNEGASGRVDSQPHNKNSG 553
             Q+    E +SG++ + PH ++ G
Sbjct: 142 SPQHFVPAEDSSGKLSALPHVESPG 166