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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK948027
Clone id TST38A01NGRL0002_D14
Library
Length 447
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0002_D14. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GTTCCAGTGACAGCTATACTTCTGAGGAGGATGATAGCCAGTTTTTTCCGAAGAGAATTG
CTTTCGATTATGGTTTTCAAGCTAAATTTCTTAGGAAAGGCCCTACCGTTCCTGGGAATG
TGTTCAAGCTTGCGTTTGAGAATTTTGGCAGAGAGTGGACGGCTTTGCGGCGAACCTATT
TATTTGCCAAATTGGAGAAAATTAAGATAAACCAGATCGAAGGAGGTCCTCTAGGGATTG
TTTCCGTTAATTCAAGCCGAGGGTTTGTGTTATTTTTGAGGGGGTTTGACAAATTACTGC
AGTTGTATGATGGACTTCCAAAAATAAACCCCCATATACGGGTCGTGTAGGAGGAAGTGG
ATGACTTACAAAAAACTTTCAAGAAGTTGACACTCAGCAAGGATGCACTCTGGGAAAGAG
ACAAAAATCGCCCTCCGGTGGAACCAC
■■Homology search results ■■ -
sp_hit_id Q87AW7
Definition sp|Q87AW7|PBPA_XYLFT Penicillin-binding protein 1A OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964)
Align length 61
Score (bit) 32.3
E-value 0.93
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK948027|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0002_D14, 5'
         (447 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q87AW7|PBPA_XYLFT Penicillin-binding protein 1A OS=Xylella fa...    32   0.93 
sp|A4VIX2|LEPA_PSEU5 GTP-binding protein lepA OS=Pseudomonas stu...    30   3.5  
sp|P11557|DAMX_ECOLI Protein damX OS=Escherichia coli (strain K1...    30   3.5  
sp|Q8X826|DAMX_ECO57 Protein damX OS=Escherichia coli O157:H7 GN...    30   3.5  
sp|Q97C04|GCSPB_THEVO Probable glycine dehydrogenase [decarboxyl...    30   4.6  
sp|P20824|NUC1_NEUCR Phosphorus acquisition-controlling protein ...    30   6.0  
sp|A8MIA8|HSLO_ALKOO 33 kDa chaperonin OS=Alkaliphilus oremlandi...    30   6.0  
sp|Q9HII2|GCSPB_THEAC Probable glycine dehydrogenase [decarboxyl...    30   6.0  
sp|O60141|CWC16_SCHPO Protein cwc16 OS=Schizosaccharomyces pombe...    30   6.0  
sp|Q6MD97|CLPB_PARUW Chaperone protein clpB OS=Protochlamydia am...    29   7.8  

>sp|Q87AW7|PBPA_XYLFT Penicillin-binding protein 1A OS=Xylella
           fastidiosa (strain Temecula1 / ATCC 700964) GN=mrcA PE=3
           SV=1
          Length = 809

 Score = 32.3 bits (72), Expect = 0.93
 Identities = 23/61 (37%), Positives = 31/61 (50%), Gaps = 7/61 (11%)
 Frame = -3

Query: 379 KVFCKSSTSSYTT-------RIWGFIFGSPSYNCSNLSNPLKNNTNPRLELTETIPRGPP 221
           KV C S TS+ T         + GF FG+P+   +  S P+KNN  P    T+TIP   P
Sbjct: 603 KVACSSCTSANTNVTQSTSHLVDGFNFGAPTK--TEPSKPVKNNNEP----TQTIPSPDP 656

Query: 220 S 218
           +
Sbjct: 657 N 657


>sp|A4VIX2|LEPA_PSEU5 GTP-binding protein lepA OS=Pseudomonas
           stutzeri (strain A1501) GN=lepA PE=3 SV=1
          Length = 599

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 22/69 (31%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
 Frame = +3

Query: 207 INQIEGGPLGIVSVNSSRGFVLFLRGFDKLL-QLYDGLPKINPHIRVV*EEVDDLQKTFK 383
           I  I G P+G     SS   V  L GF ++  Q+Y GL  ++       ++ +D ++  +
Sbjct: 265 IKDIHGAPVGDTLTLSSTPDVEMLPGFQRIKPQVYAGLFPVSS------DDFEDFREALQ 318

Query: 384 KLTLSKDAL 410
           KLTL+  AL
Sbjct: 319 KLTLNDAAL 327


>sp|P11557|DAMX_ECOLI Protein damX OS=Escherichia coli (strain K12)
           GN=damX PE=1 SV=2
          Length = 428

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 25/75 (33%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
 Frame = -3

Query: 367 KSSTSS-YTTRIWGFIFGSPSYNCSNLSNPLKNNTNPRLELTETIPRGPPSIWFILIFSN 191
           KS+ SS YT ++      S S N  NL+   K        + ET   G P  W++L+   
Sbjct: 341 KSAPSSHYTLQL------SSSSNYDNLNGWAKKENLKNYVVYETTRNGQP--WYVLVSGV 392

Query: 190 LANK*VRRKAVHSLP 146
            A+K   +KAV +LP
Sbjct: 393 YASKEEAKKAVSTLP 407


>sp|Q8X826|DAMX_ECO57 Protein damX OS=Escherichia coli O157:H7
           GN=damX PE=4 SV=1
          Length = 428

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 25/75 (33%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
 Frame = -3

Query: 367 KSSTSS-YTTRIWGFIFGSPSYNCSNLSNPLKNNTNPRLELTETIPRGPPSIWFILIFSN 191
           KS+ SS YT ++      S S N  NL+   K        + ET   G P  W++L+   
Sbjct: 341 KSAPSSHYTLQL------SSSSNYDNLNGWAKKENLKNYVVYETTRNGQP--WYVLVSGV 392

Query: 190 LANK*VRRKAVHSLP 146
            A+K   +KAV +LP
Sbjct: 393 YASKEEAKKAVSTLP 407


>sp|Q97C04|GCSPB_THEVO Probable glycine dehydrogenase
           [decarboxylating] subunit 2 OS=Thermoplasma volcanium
           GN=gcvPB PE=3 SV=1
          Length = 472

 Score = 30.0 bits (66), Expect = 4.6
 Identities = 22/56 (39%), Positives = 29/56 (51%)
 Frame = -2

Query: 206 LNFLQFGK*IGSPQSRPLSAKILKRKLEHIPRNGRAFPKKFSLKTIIESNSLRKKL 39
           LN+ +  K IG   S   S  IL R   +I RNG    K  S + ++ SN L+KKL
Sbjct: 308 LNY-ELKKTIGKVSSFYGSFSILLRAWSYIIRNGDDGLKNVSARAVLNSNYLKKKL 362


>sp|P20824|NUC1_NEUCR Phosphorus acquisition-controlling protein
           OS=Neurospora crassa GN=nuc-1 PE=4 SV=1
          Length = 823

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 19/48 (39%), Positives = 29/48 (60%)
 Frame = -2

Query: 179 IGSPQSRPLSAKILKRKLEHIPRNGRAFPKKFSLKTIIESNSLRKKLA 36
           + SPQ +P SA  LKR  +  P  GR+  K+ S+ +I  S +LR K++
Sbjct: 569 LSSPQLKPDSAHSLKRTPQLAPM-GRSSKKRASVTSIQMSPALRPKIS 615


>sp|A8MIA8|HSLO_ALKOO 33 kDa chaperonin OS=Alkaliphilus oremlandii
           (strain OhILAs) GN=hslO PE=3 SV=1
          Length = 299

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 23/50 (46%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
 Frame = +3

Query: 129 LAFENFGREWTALRRTYLFAKLEKIKI--NQIEGGPLG-IVSVNSSRGFV 269
           +A    GR  TA     L +K EK KI  N   GGPLG IV V +S+G V
Sbjct: 38  VAIAALGRTLTATSMMGLMSKSEKEKITVNINGGGPLGPIVVVGNSKGIV 87


>sp|Q9HII2|GCSPB_THEAC Probable glycine dehydrogenase
           [decarboxylating] subunit 2 OS=Thermoplasma acidophilum
           GN=gcvPB PE=3 SV=1
          Length = 472

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
 Frame = -2

Query: 200 FLQFGK*--IGSPQSRPLSAKILKRKLEHIPRNGRAFPKKFSLKTIIESNSLRKKL 39
           FL +GK   IG   S   S  IL R   ++ RNG    K  +++ ++ SN L+KK+
Sbjct: 307 FLDYGKKKSIGRVSSFYGSFSILLRAWSYVIRNGDDGLKNATIRAVLNSNYLKKKV 362


>sp|O60141|CWC16_SCHPO Protein cwc16 OS=Schizosaccharomyces pombe
           GN=cwc16 PE=2 SV=1
          Length = 294

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = -3

Query: 367 KSSTSSYTTRIWGFIFGSPSYNCSNLSNPLKNNTNPR 257
           K   S YTT+IW F     S  C   SNP+  +T+P+
Sbjct: 51  KEIGSYYTTKIWSF-----SLKCHLCSNPIDVHTDPK 82


>sp|Q6MD97|CLPB_PARUW Chaperone protein clpB OS=Protochlamydia
           amoebophila (strain UWE25) GN=clpB PE=3 SV=1
          Length = 868

 Score = 29.3 bits (64), Expect = 7.8
 Identities = 28/104 (26%), Positives = 47/104 (45%), Gaps = 4/104 (3%)
 Frame = +3

Query: 87  FLRKGPTVPGNVF---KLAFENFGREWTALRRTYLFAKLEKIKINQIEGGPLGIVSVNSS 257
           FL  GPT  G       LAF+ F ++  AL R  +   +EK  ++++ G P G +     
Sbjct: 602 FLFLGPTGVGKTELAKALAFQLFNQD-EALIRLDMSEYMEKHTVSKLIGSPPGYIGYEEG 660

Query: 258 RGFVLFLRGFDKLLQLYDGLPKINPHI-RVV*EEVDDLQKTFKK 386
                 LR     + L+D + K +P +  ++ +  DD + T  K
Sbjct: 661 GQLTEALRRRPYAVVLFDEIEKAHPDVFNILLQVFDDGRLTDSK 704
tr_hit_id Q8S308
Definition tr|Q8S308|Q8S308_CHLRE Quinol-to-oxygen oxidoreductase OS=Chlamydomonas reinhardtii
Align length 147
Score (bit) 35.4
E-value 1.4
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK948027|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0002_D14, 5'
         (447 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q8S308|Q8S308_CHLRE Quinol-to-oxygen oxidoreductase OS=Chlamy...    35   1.4  
tr|A8IU73|A8IU73_CHLRE Alternative oxidase OS=Chlamydomonas rein...    35   1.4  
tr|B8LD44|B8LD44_THAPS Predicted protein OS=Thalassiosira pseudo...    35   2.4  
tr|A5BB13|A5BB13_VITVI Putative uncharacterized protein OS=Vitis...    35   2.4  
tr|A7P833|A7P833_VITVI Chromosome chr3 scaffold_8, whole genome ...    34   3.1  
tr|A8HK52|A8HK52_HAEPL Plastid terminal oxidase OS=Haematococcus...    33   5.3  
tr|A5GZV5|A5GZV5_HAEPL Plastid terminal oxidase OS=Haematococcus...    33   5.3  
tr|B4N733|B4N733_DROWI GK23609 OS=Drosophila willistoni GN=GK236...    33   5.3  
tr|B5XTU8|B5XTU8_KLEP3 DamX protein OS=Klebsiella pneumoniae (st...    33   9.1  

>tr|Q8S308|Q8S308_CHLRE Quinol-to-oxygen oxidoreductase
           OS=Chlamydomonas reinhardtii PE=2 SV=1
          Length = 471

 Score = 35.4 bits (80), Expect = 1.4
 Identities = 32/147 (21%), Positives = 58/147 (39%), Gaps = 18/147 (12%)
 Frame = +3

Query: 45  FPKRIAFDYGFQAKFLR-----KGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKI 209
           F + +  DYGF++   R         +P N F+L ++NF  E  +LR++  F     I  
Sbjct: 92  FMEVMCCDYGFRSGASRMYQQENDGRIPKNAFQLGWDNFKSELRSLRQSIRFDDYSTISE 151

Query: 210 NQIEGGPLGIVSVNSSRGFVLFLRGFDKLLQLYDGLPKINPHIRVV*EEVDD-------- 365
                 P+   + +  +  +  L   D+ L+    L  + P    +     D        
Sbjct: 152 ANPPRTPMSRAAYSVGQAALDSLDAVDRALEAAQVLEPLRPRAVTLDGVTRDSESGSKLI 211

Query: 366 -----LQKTFKKLTLSKDALWERDKNR 431
                ++   K+L LS  A+W+R+  R
Sbjct: 212 NNCAEIRSKLKQLKLSNKAMWDREHAR 238


>tr|A8IU73|A8IU73_CHLRE Alternative oxidase OS=Chlamydomonas
           reinhardtii GN=PTOX1 PE=4 SV=1
          Length = 471

 Score = 35.4 bits (80), Expect = 1.4
 Identities = 32/147 (21%), Positives = 58/147 (39%), Gaps = 18/147 (12%)
 Frame = +3

Query: 45  FPKRIAFDYGFQAKFLR-----KGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKI 209
           F + +  DYGF++   R         +P N F+L ++NF  E  +LR++  F     I  
Sbjct: 92  FMEVMCCDYGFRSGASRMYQQENDGRIPKNAFQLGWDNFKSELRSLRQSIRFDDYSTISE 151

Query: 210 NQIEGGPLGIVSVNSSRGFVLFLRGFDKLLQLYDGLPKINPHIRVV*EEVDD-------- 365
                 P+   + +  +  +  L   D+ L+    L  + P    +     D        
Sbjct: 152 ANPPRTPMSRAAYSVGQAALDSLDAVDRALEAAQVLEPLRPRAVTLDGVTRDSESGSKLI 211

Query: 366 -----LQKTFKKLTLSKDALWERDKNR 431
                ++   K+L LS  A+W+R+  R
Sbjct: 212 NNCAEIRSKLKQLKLSNKAMWDREHAR 238


>tr|B8LD44|B8LD44_THAPS Predicted protein OS=Thalassiosira
           pseudonana CCMP1335 GN=THAPSDRAFT_25462 PE=4 SV=1
          Length = 636

 Score = 34.7 bits (78), Expect = 2.4
 Identities = 24/76 (31%), Positives = 37/76 (48%), Gaps = 3/76 (3%)
 Frame = +3

Query: 222 GGPLGIVSVNSSRGFVLFLRGFDKLLQLY---DGLPKINPHIRVV*EEVDDLQKTFKKLT 392
           G P  + S+ S +    F+R  + +L  Y   +  P + P  R       +LQ    +LT
Sbjct: 207 GPPGNVFSLGSQQ----FMRNLNAMLDEYKDEEDNPDLTPRQR-------ELQAKLNELT 255

Query: 393 LSKDALWERDKNRPPV 440
           LS DA+WER++ R  V
Sbjct: 256 LSSDAIWERERARGEV 271


>tr|A5BB13|A5BB13_VITVI Putative uncharacterized protein OS=Vitis
            vinifera GN=VITISV_038780 PE=4 SV=1
          Length = 1206

 Score = 34.7 bits (78), Expect = 2.4
 Identities = 33/106 (31%), Positives = 47/106 (44%), Gaps = 2/106 (1%)
 Frame = +3

Query: 63   FDYGFQAKFLRKGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKINQIEGGPLGIV 242
            F Y       RKG  VP  +F  A  +      A     L A  E I+  +I+G PLGIV
Sbjct: 764  FAYSVYTDMTRKG-VVPDEMFLSALID-----VAGHAGKLDAAFEVIQEARIQGIPLGIV 817

Query: 243  SVNSSRGFVLFLRGFDKLLQLYDGLP--KINPHIRVV*EEVDDLQK 374
            S +S  G     + + K L+LY  +   K+NP +  +   +  L K
Sbjct: 818  SYSSLMGACSNAKNWQKALELYVDIKSMKLNPTVSTMNALITALSK 863


>tr|A7P833|A7P833_VITVI Chromosome chr3 scaffold_8, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00036018001
           PE=4 SV=1
          Length = 757

 Score = 34.3 bits (77), Expect = 3.1
 Identities = 31/94 (32%), Positives = 43/94 (45%), Gaps = 2/94 (2%)
 Frame = +3

Query: 63  FDYGFQAKFLRKGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKINQIEGGPLGIV 242
           F Y       RKG  VP  +F  A  +      A     L A  E I+  +I+G PLGIV
Sbjct: 329 FAYSVYTDMTRKG-VVPDEMFLSALID-----VAGHAGKLDAAFEVIQEARIQGIPLGIV 382

Query: 243 SVNSSRGFVLFLRGFDKLLQLYDGLP--KINPHI 338
           S +S  G     + + K L+LY  +   K+NP +
Sbjct: 383 SYSSLMGACSNAKNWQKALELYVDIKSMKLNPTV 416


>tr|A8HK52|A8HK52_HAEPL Plastid terminal oxidase OS=Haematococcus
           pluvialis GN=PTOX1 PE=4 SV=1
          Length = 447

 Score = 33.5 bits (75), Expect = 5.3
 Identities = 38/153 (24%), Positives = 65/153 (42%), Gaps = 21/153 (13%)
 Frame = +3

Query: 45  FPKRIAFDYGFQA----KFLRKGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKIN 212
           F + +  DYG++A     +     T+P +  ++A ENF  E   LRR+  F K +   + 
Sbjct: 71  FVEVMCCDYGYRAGSGRMYNTDSGTIPMSGVQMAVENFVDELACLRRS--FRKDDYGPVA 128

Query: 213 QIEGGPLGIVSVNSS---RGFVLFLRGFDKLLQLYDGLPKINPHI--------------R 341
                  GIV    +   RG    +   D+ L+    LPK++                 R
Sbjct: 129 AKAAPHPGIVRQGFNLLGRGLTRLVAAADRSLEWLHLLPKLDEEAGQLPGTVRQGNVVKR 188

Query: 342 VV*EEVDDLQKTFKKLTLSKDALWERDKNRPPV 440
            V EE  ++++   +L LS  A+ ER++ R  +
Sbjct: 189 SVGEECAEIRQRLSRLILSNHAVSERERVREAI 221


>tr|A5GZV5|A5GZV5_HAEPL Plastid terminal oxidase OS=Haematococcus
           pluvialis GN=PTOX1 PE=2 SV=1
          Length = 447

 Score = 33.5 bits (75), Expect = 5.3
 Identities = 38/153 (24%), Positives = 65/153 (42%), Gaps = 21/153 (13%)
 Frame = +3

Query: 45  FPKRIAFDYGFQA----KFLRKGPTVPGNVFKLAFENFGREWTALRRTYLFAKLEKIKIN 212
           F + +  DYG++A     +     T+P +  ++A ENF  E   LRR+  F K +   + 
Sbjct: 71  FVEVMCCDYGYRAGSGRMYNTDSGTIPMSGVQMAVENFVDELACLRRS--FRKDDYGPVA 128

Query: 213 QIEGGPLGIVSVNSS---RGFVLFLRGFDKLLQLYDGLPKINPHI--------------R 341
                  GIV    +   RG    +   D+ L+    LPK++                 R
Sbjct: 129 AKAAPHPGIVRQGFNLLGRGLTRLVAAADRSLEWLHLLPKLDEEAGQLPGTVRQGNVVKR 188

Query: 342 VV*EEVDDLQKTFKKLTLSKDALWERDKNRPPV 440
            V EE  ++++   +L LS  A+ ER++ R  +
Sbjct: 189 SVGEECAEIRQRLSRLILSNHAVSERERVREAI 221


>tr|B4N733|B4N733_DROWI GK23609 OS=Drosophila willistoni GN=GK23609
           PE=4 SV=1
          Length = 960

 Score = 33.5 bits (75), Expect = 5.3
 Identities = 16/34 (47%), Positives = 21/34 (61%)
 Frame = -3

Query: 319 GSPSYNCSNLSNPLKNNTNPRLELTETIPRGPPS 218
           GSP  +CS+ S P K++T   LE     P+GPPS
Sbjct: 93  GSPVPSCSSPSTPTKSHTPSPLESMAHTPQGPPS 126


>tr|B5XTU8|B5XTU8_KLEP3 DamX protein OS=Klebsiella pneumoniae
           (strain 342) GN=damX PE=4 SV=1
          Length = 429

 Score = 32.7 bits (73), Expect = 9.1
 Identities = 21/57 (36%), Positives = 29/57 (50%)
 Frame = -3

Query: 316 SPSYNCSNLSNPLKNNTNPRLELTETIPRGPPSIWFILIFSNLANK*VRRKAVHSLP 146
           S S N  NL+N  K     R  + ET   G P  W++L+    A+K   ++AV SLP
Sbjct: 354 SSSSNYDNLNNWAKKEKLDRYVVYETSRNGQP--WYVLVSGIYASKEEAKRAVTSLP 408