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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK948741
Clone id TST38A01NGRL0004_B23
Library
Length 701
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0004_B23. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GCTCTCCTTCCTATTCATATGGCATGATCGAAGCTGTCCATCAGCCGCTATGGGAAGGTA
AGCTGTATAAGTTAGAAAGAGATGGTCACGCCCTTCTGATTGTCTCCTTCTCGTCGTGAA
TTGTTGAAGAACATTCGAGCGTACATGGCGAAGTTGGATTCTGCACAGAGAGAAATTTGG
CAGTTTGGTTTTCTTTAATCTGATCATACATGCAATCACTGCCAGTAGGCCTTCAATCCT
CCGGCCTCCTTTGCAAGGCATGTTGGCGTAAGCTGTATTGCCTTGGTTTTGTTTGGTTCA
AGTGCCTGAGTTTGCAGAATCAAACCTTCTTTTCTTTAGTATGCACAGATCATGCATAGT
AGCTTGCATTTACCTCCTTTTTGTTAGTTTGGGAGTGTAAACCAGAGCACATGTAAGCAT
TTTATCAATGATCTTGCGCCTCTCACTCTGTTCATTTCCATCTTTATCTCGGTGACCTTA
ACATGTGCATGCATTCCCATACAGTCACTTCAGTCATCTAATTAACCCTTACACAAATCC
CCTTTGTTTTGCTTGATTTGCGCTCATCTGGTTCACTCCACATCTTGTTGCATACCTTTC
ACAAAGCCATGTCTTCTGTTTATGTATATCANACTCTTCGTCCACATGCGATCACCTCAT
CCATTGTCGCCATGCATGCTTTCTCACAGTTGCCCACCTTT
■■Homology search results ■■ -
sp_hit_id Q8BMC0
Definition sp|Q8BMC0|P2RY5_MOUSE Oleoyl-L-alpha-lysophosphatidic acid receptor OS=Mus musculus
Align length 163
Score (bit) 31.2
E-value 5.6
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK948741|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0004_B23, 5'
         (701 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8BMC0|P2RY5_MOUSE Oleoyl-L-alpha-lysophosphatidic acid recep...    31   5.6  
sp|P43657|P2RY5_HUMAN Oleoyl-L-alpha-lysophosphatidic acid recep...    31   5.6  
sp|Q9GL50|STEA1_PIG Metalloreductase STEAP1 OS=Sus scrofa GN=STE...    31   7.4  
sp|Q4G072|P2RY5_RAT Oleoyl-L-alpha-lysophosphatidic acid recepto...    31   7.4  
sp|P32250|P2RY5_CHICK Oleoyl-L-alpha-lysophosphatidic acid recep...    31   7.4  
sp|Q58675|Y1279_METJA Uncharacterized protein MJ1279 OS=Methanoc...    30   9.6  

>sp|Q8BMC0|P2RY5_MOUSE Oleoyl-L-alpha-lysophosphatidic acid receptor
           OS=Mus musculus GN=P2ry5 PE=1 SV=1
          Length = 344

 Score = 31.2 bits (69), Expect = 5.6
 Identities = 36/163 (22%), Positives = 73/163 (44%), Gaps = 17/163 (10%)
 Frame = +1

Query: 256 RHVGVSCIAL---VLFGSSA*VCRIKPSFL*YAQIMHSSLHLPPFC*FGSVNQSTCKHFI 426
           R+  + CIA+   V+ GS+  V         + Q  HS  +      F +   +T K ++
Sbjct: 131 RNAKIVCIAVWFTVMGGSAPAV---------FFQSTHSQGNNTSEACFENFPAATWKTYL 181

Query: 427 NDLAPLTLFISIFISVTL--TCACIPIQSLQS----SN*PLHKSPLFCLICAHLVHSTSC 588
           + +      +  FI + L  TC+ + +++L      S   ++K+ +  +I  HLV    C
Sbjct: 182 SRIVIFIEIVGFFIPLILNVTCSSMVLRTLNKPVTLSRSKMNKTKVLKMIFVHLVIFCFC 241

Query: 589 CIPFTKPCLLF--MYIXLFVH------MRSPHPLSPCMLSHSC 693
            +P+    +L+  M    FV+      +R+ +P++ C+   +C
Sbjct: 242 FVPYNINLILYSLMRTQTFVNCSVVAAVRTMYPITLCIAVSNC 284


>sp|P43657|P2RY5_HUMAN Oleoyl-L-alpha-lysophosphatidic acid receptor
           OS=Homo sapiens GN=P2RY5 PE=1 SV=3
          Length = 344

 Score = 31.2 bits (69), Expect = 5.6
 Identities = 26/132 (19%), Positives = 61/132 (46%), Gaps = 14/132 (10%)
 Frame = +1

Query: 340 YAQIMHSSLHLPPFC*FGSVNQSTCKHFINDLAPLTLFISIFISVTL--TCACIPIQSLQ 513
           + Q  HS  +      F +  ++T K +++ +      +  FI + L  TC+ + +++L 
Sbjct: 153 FVQSTHSQGNNASEACFENFPEATWKTYLSRIVIFIEIVGFFIPLILNVTCSSMVLKTLT 212

Query: 514 S----SN*PLHKSPLFCLICAHLVHSTSCCIPFTKPCLLFMYI--XLFVH------MRSP 657
                S   ++K+ +  +I  HL+    C +P+    +L+  +    FV+      +R+ 
Sbjct: 213 KPVTLSRSKINKTKVLKMIFVHLIIFCFCFVPYNINLILYSLVRTQTFVNCSVVAAVRTM 272

Query: 658 HPLSPCMLSHSC 693
           +P++ C+   +C
Sbjct: 273 YPITLCIAVSNC 284


>sp|Q9GL50|STEA1_PIG Metalloreductase STEAP1 OS=Sus scrofa GN=STEAP1
           PE=2 SV=1
          Length = 338

 Score = 30.8 bits (68), Expect = 7.4
 Identities = 29/105 (27%), Positives = 51/105 (48%)
 Frame = +2

Query: 155 WILHREKFGSLVFFNLIIHAITASRPSILRPPLQGMLA*AVLPWFCLVQVPEFAESNLLF 334
           W++ R++FG L FF  ++HA+ +     L  P++      +L W    QV +  E   + 
Sbjct: 156 WMVTRKQFGLLSFFFAVLHAVYS-----LSYPMRRSYRYKLLNW-AYQQVQQNKEDAWIE 209

Query: 335 FSMHRSCIVACIYLLFVSLGV*TRAHVSILSMILRLSLCSFPSLS 469
             + R  I       +VSLG+ T      L+++  L++ S PS+S
Sbjct: 210 HDVWRMEI-------YVSLGIVT------LAILALLAVTSIPSVS 241


>sp|Q4G072|P2RY5_RAT Oleoyl-L-alpha-lysophosphatidic acid receptor
           OS=Rattus norvegicus GN=P2ry5 PE=2 SV=1
          Length = 344

 Score = 30.8 bits (68), Expect = 7.4
 Identities = 35/163 (21%), Positives = 73/163 (44%), Gaps = 17/163 (10%)
 Frame = +1

Query: 256 RHVGVSCIAL---VLFGSSA*VCRIKPSFL*YAQIMHSSLHLPPFC*FGSVNQSTCKHFI 426
           R+  + CIA+   V+ GS+  V         + Q  HS  +      F +   +T K ++
Sbjct: 131 RNAKIVCIAVWFTVMGGSAPAV---------FFQSTHSQGNNTSEACFENFPAATWKTYL 181

Query: 427 NDLAPLTLFISIFISVTL--TCACIPIQSLQS----SN*PLHKSPLFCLICAHLVHSTSC 588
           + +      +  FI + L  TC+ + +++L      S   ++K+ +  +I  HLV    C
Sbjct: 182 SRIVIFIEIVGFFIPLILNVTCSSMVLRTLNKPVTLSRSKMNKTKVLKMIFVHLVIFCFC 241

Query: 589 CIPFTKPCLLFMYI--XLFVH------MRSPHPLSPCMLSHSC 693
            +P+    +L+  +    FV+      +R+ +P++ C+   +C
Sbjct: 242 FVPYNINLILYSLVRTQTFVNCSVGAAVRTMYPITLCIAVSNC 284


>sp|P32250|P2RY5_CHICK Oleoyl-L-alpha-lysophosphatidic acid receptor
           OS=Gallus gallus GN=P2RY5 PE=1 SV=1
          Length = 308

 Score = 30.8 bits (68), Expect = 7.4
 Identities = 24/116 (20%), Positives = 52/116 (44%), Gaps = 14/116 (12%)
 Frame = +1

Query: 388 FGSVNQSTCKHFINDLAPLTLFISIFISVTL--TCACIPIQSLQS----SN*PLHKSPLF 549
           F +  +ST K +++ +      +  FI + L  TC+ + +++L      S   L K  + 
Sbjct: 166 FENFPESTWKTYLSRIVIFIEIVGFFIPLILNVTCSTMVLRTLNKPLTLSRNKLSKKKVL 225

Query: 550 CLICAHLVHSTSCCIPFTKPCLLFMYI--------XLFVHMRSPHPLSPCMLSHSC 693
            +I  HLV    C +P+    +L+  +         +   +R+ +P++ C+   +C
Sbjct: 226 KMIFVHLVIFCFCFVPYNITLILYSLMRTQTWINCSVVTAVRTMYPVTLCIAVSNC 281


>sp|Q58675|Y1279_METJA Uncharacterized protein MJ1279
           OS=Methanocaldococcus jannaschii GN=MJ1279 PE=4 SV=1
          Length = 185

 Score = 30.4 bits (67), Expect = 9.6
 Identities = 12/44 (27%), Positives = 25/44 (56%)
 Frame = +3

Query: 525 TLTQIPFVLLDLRSSGSLHILLHTFHKAMSSVYVYXTLRPHAIT 656
           TLT   +VL+D+ S+G+ H  +    ++   + +   + PH++T
Sbjct: 40  TLTDNGYVLMDIASNGNFHYFMFEHLESWDRIKIVAEVLPHSLT 83
tr_hit_id A0EH89
Definition tr|A0EH89|A0EH89_PARTE Chromosome undetermined scaffold_96, whole genome shotgun sequence OS=Paramecium tetraurelia
Align length 87
Score (bit) 38.5
E-value 0.42
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK948741|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0004_B23, 5'
         (701 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A0EH89|A0EH89_PARTE Chromosome undetermined scaffold_96, whol...    39   0.42 
tr|B0DKR5|B0DKR5_LACBS Predicted protein OS=Laccaria bicolor (st...    37   1.6  
tr|B3S3E0|B3S3E0_TRIAD Putative uncharacterized protein OS=Trich...    36   2.7  
tr|B2VH70|B2VH70_ERWT9 Putative membrane protein OS=Erwinia tasm...    35   6.1  
tr|B1DF86|B1DF86_9BACL Putative uncharacterized protein OS=Paeni...    35   6.1  
tr|B8MCC4|B8MCC4_9EURO Putative uncharacterized protein OS=Talar...    35   6.1  

>tr|A0EH89|A0EH89_PARTE Chromosome undetermined scaffold_96, whole
           genome shotgun sequence OS=Paramecium tetraurelia
           GN=GSPATT00027004001 PE=4 SV=1
          Length = 1896

 Score = 38.5 bits (88), Expect = 0.42
 Identities = 27/87 (31%), Positives = 32/87 (36%), Gaps = 10/87 (11%)
 Frame = +1

Query: 382 C*FGSVNQSTCKHFINDLAPLTLFISIFISVTLTCACIPIQSLQSSN*PLHKSPLFCLIC 561
           C   S N STC    N + P             TCAC   Q L +S+      P  C +C
Sbjct: 133 CSSSSTNCSTCVDTTNQVTP-------------TCACKEAQILNTSSYLCDSCPSNCKVC 179

Query: 562 AHLVHSTSC----------CIPFTKPC 612
           +     T C          CIP TKPC
Sbjct: 180 SSTSVCTQCYDGYLLSATVCIPCTKPC 206


>tr|B0DKR5|B0DKR5_LACBS Predicted protein OS=Laccaria bicolor
           (strain S238N-H82) GN=LACBIDRAFT_330268 PE=4 SV=1
          Length = 158

 Score = 36.6 bits (83), Expect = 1.6
 Identities = 22/46 (47%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
 Frame = +1

Query: 388 FGSVNQSTC-KHFINDLAPLTLFISIFISVTLTCACIPIQSLQSSN 522
           F  +N   C KH I DL  L    S+ ISVT TCAC  IQ L+  N
Sbjct: 67  FERINLLVCRKHHIRDLGVLVCMFSVSISVT-TCACYDIQLLKLGN 111


>tr|B3S3E0|B3S3E0_TRIAD Putative uncharacterized protein
           OS=Trichoplax adhaerens GN=TRIADDRAFT_58684 PE=4 SV=1
          Length = 388

 Score = 35.8 bits (81), Expect = 2.7
 Identities = 35/139 (25%), Positives = 61/139 (43%), Gaps = 12/139 (8%)
 Frame = +1

Query: 262 VGVSCIALVLFGSSA*VCRIKPSFL*YAQIMHSSLHLPPFC*FGSVNQSTCKHFINDLAP 441
           VG   + LVLFG S     +  S + +A  +HS   LP F   G+      + F+N   P
Sbjct: 87  VGFDLLCLVLFGVSEITNGLSGSQI-FAVALHSRAVLPGFIWIGASLLIFMRRFLNRFIP 145

Query: 442 -LTLFISIFISVTLTCACIPIQSLQSSN*PLHK------SPLFCLIC-----AHLVHSTS 585
            L+L   + + VT+ C  +   ++ +   P+H       SPL  ++      A L+    
Sbjct: 146 VLSLIPLVILIVTVVCCLVVGNAIHN---PIHSSWEYGASPLLIVLASLGGTARLMPYLV 202

Query: 586 CCIPFTKPCLLFMYIXLFV 642
             I F +  L++ Y+ + +
Sbjct: 203 NFIQFERKSLIYFYLSIIL 221


>tr|B2VH70|B2VH70_ERWT9 Putative membrane protein OS=Erwinia
           tasmaniensis (strain DSM 17950 / Et1/99) GN=ETA_06140
           PE=4 SV=1
          Length = 1209

 Score = 34.7 bits (78), Expect = 6.1
 Identities = 17/29 (58%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
 Frame = +1

Query: 109 SRRELLKNIRAYMAKLDSAQRE-IWQFGF 192
           ++ +LLK  RAY AK D AQRE IW F F
Sbjct: 281 TKADLLKGFRAYFAKFDKAQREQIWGFTF 309


>tr|B1DF86|B1DF86_9BACL Putative uncharacterized protein
           OS=Paenibacillus sp. JDR-2 GN=Pjdr2DRAFT_3774 PE=4 SV=1
          Length = 304

 Score = 34.7 bits (78), Expect = 6.1
 Identities = 25/71 (35%), Positives = 36/71 (50%), Gaps = 3/71 (4%)
 Frame = -3

Query: 207 MIRLKKTKLPN--FSLCRIQLRHVR-SNVLQQFTTRRRQSEGRDHLFLTYTAYLPIAADG 37
           M++LKK  LP+  F L  I +  V  S++L +F+       G   LF+T    LP     
Sbjct: 1   MLKLKKASLPSHPFVLLFISILSVSVSSILIKFSDTPTSVAGMYRLFITVIIMLPFVPWR 60

Query: 36  QLRSCHMNRKE 4
           QLRS  +N K+
Sbjct: 61  QLRSLKLNMKD 71


>tr|B8MCC4|B8MCC4_9EURO Putative uncharacterized protein
           OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_122990 PE=4
           SV=1
          Length = 897

 Score = 34.7 bits (78), Expect = 6.1
 Identities = 27/78 (34%), Positives = 38/78 (48%)
 Frame = +1

Query: 61  SCIS*KEMVTPF*LSPSRRELLKNIRAYMAKLDSAQREIWQFGFL*SDHTCNHCQ*AFNP 240
           +C+S ++  TP  L  SR +   ++R    ++ +AQ      GF     TC  CQ    P
Sbjct: 303 ACLS-RDCPTPSKLYESRHQWSHHMREIHPEVAAAQN-----GF-----TCPLCQINVQP 351

Query: 241 PASFARHVGVSCIALVLF 294
           PASF +HVG     L LF
Sbjct: 352 PASFEKHVGRHLEELALF 369