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DK950559
Clone id TST38A01NGRL0008_P18
Library
Length 432
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_P18. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GGATGAGGTTACTTCTCCAGCAGGTACCACAATTGCAGGAATTAGAGCTTTAGAAAAGGC
AGGTTTCCGAGCGGCCCTCATAGATGCTGTCGTAGCTGCAGCGAAGAGAGGTGAAGAGCT
TTCAAAATCTTAGGATCGAAGCATCTATTCTGATGCCAGATTCTTGACAAATTTATGATG
TGACAGTGATCACGTCAAAAATTAGTTCAGGCTTTTATTTTGCTAAGCGAGCCTATACAG
CAGGAAAACTCTTTTGGAACATGAGATCTTTTTCTGCCAACTTTACAAAAGTAAGGCTGC
TAGTTCAGGGTAACAAGCATATAATCTGACTACAATAGATAACATTTACAACTTTGATTT
ATTAAAAAATTGTAGTGGGGGCAGTTTATTAACAGGGCGTTTTGGAGACNAAAAAAAAAA
AAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id Q04708
Definition sp|Q04708|P5CR_PEA Pyrroline-5-carboxylate reductase OS=Pisum sativum
Align length 41
Score (bit) 44.3
E-value 0.0002
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950559|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0008_P18, 5'
         (410 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q04708|P5CR_PEA Pyrroline-5-carboxylate reductase OS=Pisum sa...    44   2e-04
sp|P54904|P5CR1_ARATH Pyrroline-5-carboxylate reductase OS=Arabi...    44   3e-04
sp|P74572|P5CR_SYNY3 Pyrroline-5-carboxylate reductase OS=Synech...    44   3e-04
sp|P17817|P5CR_SOYBN Pyrroline-5-carboxylate reductase OS=Glycin...    41   0.002
sp|Q9HH99|P5CR_METAC Pyrroline-5-carboxylate reductase OS=Methan...    40   0.003
sp|Q20848|P5CR_CAEEL Putative pyrroline-5-carboxylate reductase ...    40   0.003
sp|O04016|P5CR_ACTCH Pyrroline-5-carboxylate reductase OS=Actini...    39   0.006
sp|P0A9L9|P5CR_SHIFL Pyrroline-5-carboxylate reductase OS=Shigel...    38   0.018
sp|P0A9L8|P5CR_ECOLI Pyrroline-5-carboxylate reductase OS=Escher...    38   0.018
sp|P0C1E5|P5CR_CORML Pyrroline-5-carboxylate reductase OS=Coryne...    37   0.024
sp|P0C1E4|P5CR_CORGL Pyrroline-5-carboxylate reductase OS=Coryne...    37   0.024
sp|Q9DCC4|P5CR3_MOUSE Pyrroline-5-carboxylate reductase 3 OS=Mus...    37   0.024
sp|Q5PQJ6|P5CR3_RAT Pyrroline-5-carboxylate reductase 3 OS=Rattu...    37   0.031
sp|P54893|P5CR_THET2 Pyrroline-5-carboxylate reductase OS=Thermu...    37   0.041
sp|Q5SPD7|P5CR3_DANRE Pyrroline-5-carboxylate reductase 3 OS=Dan...    37   0.041
sp|P14383|P5CR1_BACSU Pyrroline-5-carboxylate reductase 1 OS=Bac...    35   0.090
sp|Q11141|P5CR_MYCTU Pyrroline-5-carboxylate reductase OS=Mycoba...    35   0.15 
sp|P54552|P5CR2_BACSU Pyrroline-5-carboxylate reductase 2 OS=Bac...    33   0.45 
sp|O66553|P5CR_AQUAE Pyrroline-5-carboxylate reductase OS=Aquife...    33   0.59 
sp|Q53H96|P5CR3_HUMAN Pyrroline-5-carboxylate reductase 3 OS=Hom...    32   1.00 
sp|P27771|P5CR_TREPA Pyrroline-5-carboxylate reductase OS=Trepon...    32   1.3  
sp|Q4R531|P5CR3_MACFA Pyrroline-5-carboxylate reductase 3 OS=Mac...    32   1.3  
sp|Q54IL7|P5CR1_DICDI Pyrroline-5-carboxylate reductase 1 OS=Dic...    32   1.3  
sp|P46725|P5CR_MYCLE Pyrroline-5-carboxylate reductase OS=Mycoba...    31   2.2  
sp|Q58D08|P5CR3_BOVIN Pyrroline-5-carboxylate reductase 3 OS=Bos...    30   2.9  
sp|Q5R9X6|P5CR1_PONAB Pyrroline-5-carboxylate reductase 1, mitoc...    30   3.8  
sp|P32322|P5CR1_HUMAN Pyrroline-5-carboxylate reductase 1, mitoc...    30   3.8  
sp|Q58DT4|P5CR1_BOVIN Pyrroline-5-carboxylate reductase 1, mitoc...    30   3.8  
sp|Q922W5|P5CR1_MOUSE Pyrroline-5-carboxylate reductase 1, mitoc...    30   5.0  
sp|A1L2Q8|P5CR3_XENLA Pyrroline-5-carboxylate reductase 3 OS=Xen...    29   8.5  

>sp|Q04708|P5CR_PEA Pyrroline-5-carboxylate reductase OS=Pisum
           sativum GN=PROC PE=2 SV=1
          Length = 273

 Score = 44.3 bits (103), Expect = 2e-04
 Identities = 22/41 (53%), Positives = 24/41 (58%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELS 124
           D+VTSP GTTIAG+  LEK GFR           KR  ELS
Sbjct: 233 DDVTSPGGTTIAGVHELEKGGFRGTLMNAVVAAAKRSRELS 273


>sp|P54904|P5CR1_ARATH Pyrroline-5-carboxylate reductase
           OS=Arabidopsis thaliana GN=PROC1 PE=2 SV=1
          Length = 276

 Score = 43.9 bits (102), Expect = 3e-04
 Identities = 22/43 (51%), Positives = 25/43 (58%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D+VTSP GTTIAG+  LEK  FR           KR  ELS+S
Sbjct: 234 DDVTSPGGTTIAGVHELEKGSFRATLMNAVVAAAKRSRELSQS 276


>sp|P74572|P5CR_SYNY3 Pyrroline-5-carboxylate reductase
           OS=Synechocystis sp. (strain PCC 6803) GN=proC PE=3 SV=1
          Length = 267

 Score = 43.5 bits (101), Expect = 3e-04
 Identities = 21/42 (50%), Positives = 25/42 (59%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D+VTSP GTTIAG+  LEK GFR           +R +EL K
Sbjct: 225 DKVTSPGGTTIAGVAVLEKMGFRSAIIEAVRAAYRRSQELGK 266


>sp|P17817|P5CR_SOYBN Pyrroline-5-carboxylate reductase OS=Glycine
           max PE=2 SV=1
          Length = 274

 Score = 41.2 bits (95), Expect = 0.002
 Identities = 21/41 (51%), Positives = 22/41 (53%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELS 124
           D+VTSP GTTI GI  LE  GFR           KR  ELS
Sbjct: 234 DDVTSPGGTTITGIHELENGGFRGTLMNAVVAAAKRSRELS 274


>sp|Q9HH99|P5CR_METAC Pyrroline-5-carboxylate reductase
           OS=Methanosarcina acetivorans GN=proC PE=3 SV=1
          Length = 270

 Score = 40.4 bits (93), Expect = 0.003
 Identities = 21/42 (50%), Positives = 25/42 (59%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D VTSPAGTTI GI +LE+AG R           +R +EL K
Sbjct: 228 DMVTSPAGTTIQGIHSLEEAGIRAAFMNAVIRASERSKELGK 269


>sp|Q20848|P5CR_CAEEL Putative pyrroline-5-carboxylate reductase
           OS=Caenorhabditis elegans GN=F55G1.9 PE=2 SV=1
          Length = 299

 Score = 40.4 bits (93), Expect = 0.003
 Identities = 20/43 (46%), Positives = 25/43 (58%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D V SP GTTI G+RALEK GFR            + +E++KS
Sbjct: 254 DMVCSPGGTTIEGVRALEKNGFRYAVMEAVVAASTKADEMAKS 296


>sp|O04016|P5CR_ACTCH Pyrroline-5-carboxylate reductase OS=Actinidia
           chinensis PE=2 SV=1
          Length = 278

 Score = 39.3 bits (90), Expect = 0.006
 Identities = 20/42 (47%), Positives = 23/42 (54%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D+V S  GTTIAGI  LEK GFR           KR +E+ K
Sbjct: 236 DDVASAGGTTIAGIHELEKGGFRGTLMNAVVSATKRSQEIFK 277


>sp|P0A9L9|P5CR_SHIFL Pyrroline-5-carboxylate reductase OS=Shigella
           flexneri GN=proC PE=3 SV=1
          Length = 269

 Score = 37.7 bits (86), Expect = 0.018
 Identities = 19/43 (44%), Positives = 24/43 (55%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D V SP GTTI  +R LE+ GFR           ++ E+LSKS
Sbjct: 227 DMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS 269


>sp|P0A9L8|P5CR_ECOLI Pyrroline-5-carboxylate reductase
           OS=Escherichia coli (strain K12) GN=proC PE=1 SV=1
          Length = 269

 Score = 37.7 bits (86), Expect = 0.018
 Identities = 19/43 (44%), Positives = 24/43 (55%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D V SP GTTI  +R LE+ GFR           ++ E+LSKS
Sbjct: 227 DMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS 269


>sp|P0C1E5|P5CR_CORML Pyrroline-5-carboxylate reductase
           OS=Corynebacterium melassecola GN=proC PE=3 SV=1
          Length = 270

 Score = 37.4 bits (85), Expect = 0.024
 Identities = 19/40 (47%), Positives = 23/40 (57%)
 Frame = +2

Query: 8   VTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           V+SPAGTT+A IR LE++G R            R EEL K
Sbjct: 230 VSSPAGTTVAAIRELEESGIRGAFYRAAQACADRSEELGK 269
tr_hit_id A9NNU3
Definition tr|A9NNU3|A9NNU3_PICSI Pyrroline-5-carboxylate reductase OS=Picea sitchensis
Align length 43
Score (bit) 48.1
E-value 0.0002
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950559|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0008_P18, 5'
         (410 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9NNU3|A9NNU3_PICSI Pyrroline-5-carboxylate reductase OS=Pice...    48   2e-04
tr|A9T0D9|A9T0D9_PHYPA Pyrroline-5-carboxylate reductase OS=Phys...    47   4e-04
tr|A2Q2Y7|A2Q2Y7_MEDTR Pyrroline-5-carboxylate reductase OS=Medi...    47   6e-04
tr|Q8GE04|Q8GE04_HELMO Pyrroline-5-carboxylate reductase (Fragme...    45   0.002
tr|B0TGN7|B0TGN7_HELMI Pyrroline-5-carboxylate reductase OS=Heli...    44   0.005
tr|A0ZMZ3|A0ZMZ3_NODSP Pyrroline-5-carboxylate reductase OS=Nodu...    44   0.005
tr|Q8YZH2|Q8YZH2_ANASP Pyrroline-5-carboxylate reductase OS=Anab...    43   0.007
tr|Q3M924|Q3M924_ANAVT Pyrroline-5-carboxylate reductase OS=Anab...    43   0.007
tr|B2J1K7|B2J1K7_NOSP7 Pyrroline-5-carboxylate reductase OS=Nost...    43   0.007
tr|Q4TZJ2|Q4TZJ2_MAIZE Pyrroline-5-carboxylate reductase OS=Zea ...    43   0.007
tr|Q10V93|Q10V93_TRIEI Pyrroline-5-carboxylate reductase OS=Tric...    42   0.012
tr|Q84UC6|Q84UC6_HORVD Pyrroline-5-carboxylate reductase OS=Hord...    42   0.012
tr|Q5EI64|Q5EI64_WHEAT Pyrroline-5-carboxylate reductase OS=Trit...    42   0.012
tr|B7KDJ1|B7KDJ1_9CHRO Pyrroline-5-carboxylate reductase OS=Cyan...    42   0.015
tr|Q8GT01|Q8GT01_ORYSJ Pyrroline-5-carboxylate reductase OS=Oryz...    42   0.015
tr|Q5JMU4|Q5JMU4_ORYSJ Putative pyrroline-5-carboxylate reductas...    42   0.015
tr|Q0JG21|Q0JG21_ORYSJ Pyrroline-5-carboxylate reductase (Fragme...    42   0.015
tr|B8C4H2|B8C4H2_THAPS Pyrroline-5-carboxylate reductase OS=Thal...    42   0.015
tr|B8A957|B8A957_ORYSI Putative uncharacterized protein OS=Oryza...    42   0.015
tr|A3A1I2|A3A1I2_ORYSJ Putative uncharacterized protein OS=Oryza...    42   0.015
tr|A8X1F4|A8X1F4_CAEBR Pyrroline-5-carboxylate reductase OS=Caen...    42   0.015
tr|A5GB68|A5GB68_GEOUR Pyrroline-5-carboxylate reductase OS=Geob...    42   0.020
tr|B7JW57|B7JW57_SYNP8 Pyrroline-5-carboxylate reductase OS=Syne...    42   0.020
tr|B4C0H3|B4C0H3_9CHRO Pyrroline-5-carboxylate reductase OS=Cyan...    42   0.020
tr|A7QJL9|A7QJL9_VITVI Pyrroline-5-carboxylate reductase OS=Viti...    42   0.020
tr|Q7U8F7|Q7U8F7_SYNPX Pyrroline-5-carboxylate reductase OS=Syne...    41   0.026
tr|Q1J041|Q1J041_DEIGD Pyrroline-5-carboxylate reductase OS=Dein...    41   0.026
tr|B7AZG4|B7AZG4_9FIRM Putative uncharacterized protein OS=Mitsu...    41   0.026
tr|A3IWN8|A3IWN8_9CHRO Pyrroline-5-carboxylate reductase OS=Cyan...    41   0.026
tr|Q9AYM3|Q9AYM3_VIGUN Pyrroline-5-carboxylate reductase OS=Vign...    41   0.026

>tr|A9NNU3|A9NNU3_PICSI Pyrroline-5-carboxylate reductase OS=Picea
           sitchensis PE=2 SV=1
          Length = 272

 Score = 48.1 bits (113), Expect = 2e-04
 Identities = 25/43 (58%), Positives = 26/43 (60%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           DEV SPAGTTI GI  LEK GFR           KR EELSK+
Sbjct: 230 DEVASPAGTTITGIHELEKGGFRATLMSAVIAATKRSEELSKT 272


>tr|A9T0D9|A9T0D9_PHYPA Pyrroline-5-carboxylate reductase
           OS=Physcomitrella patens subsp. patens
           GN=PHYPADRAFT_138353 PE=3 SV=1
          Length = 271

 Score = 47.4 bits (111), Expect = 4e-04
 Identities = 24/41 (58%), Positives = 27/41 (65%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELS 124
           D VTSPAGTTIAG+R LE+ GFR           KRGEEL+
Sbjct: 229 DMVTSPAGTTIAGVRELEERGFRSALINAVLAGTKRGEELA 269


>tr|A2Q2Y7|A2Q2Y7_MEDTR Pyrroline-5-carboxylate reductase
           OS=Medicago truncatula GN=MtrDRAFT_AC153128g5v2 PE=3
           SV=1
          Length = 303

 Score = 46.6 bits (109), Expect = 6e-04
 Identities = 23/41 (56%), Positives = 26/41 (63%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELS 124
           D+VTSP GTTIAG+  LEKAGFR           KR +ELS
Sbjct: 263 DDVTSPGGTTIAGVHELEKAGFRGILMNAVVAAAKRSQELS 303


>tr|Q8GE04|Q8GE04_HELMO Pyrroline-5-carboxylate reductase (Fragment)
           OS=Heliobacillus mobilis PE=4 SV=1
          Length = 274

 Score = 44.7 bits (104), Expect = 0.002
 Identities = 23/43 (53%), Positives = 26/43 (60%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D+VTSP GTTIAG++ALE  GFR           KR  ELS S
Sbjct: 231 DKVTSPGGTTIAGVQALEDGGFRSAVFAAVHAGTKRARELSGS 273


>tr|B0TGN7|B0TGN7_HELMI Pyrroline-5-carboxylate reductase
           OS=Heliobacterium modesticaldum (strain ATCC 51547 /
           Ice1) GN=proC PE=4 SV=1
          Length = 276

 Score = 43.5 bits (101), Expect = 0.005
 Identities = 22/43 (51%), Positives = 26/43 (60%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSKS 130
           D+VTSP GTTIAG++ALE  GFR           +R  ELS S
Sbjct: 233 DKVTSPGGTTIAGVQALEDGGFRSSVFAAVHAATRRARELSGS 275


>tr|A0ZMZ3|A0ZMZ3_NODSP Pyrroline-5-carboxylate reductase
           OS=Nodularia spumigena CCY 9414 GN=N9414_22988 PE=3 SV=1
          Length = 270

 Score = 43.5 bits (101), Expect = 0.005
 Identities = 23/42 (54%), Positives = 24/42 (57%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D VTSP GTTIAGI  LEKAGFR            R +EL K
Sbjct: 229 DSVTSPGGTTIAGIAQLEKAGFRSAVIEAVKAAALRSQELGK 270


>tr|Q8YZH2|Q8YZH2_ANASP Pyrroline-5-carboxylate reductase
           OS=Anabaena sp. (strain PCC 7120) GN=alr0488 PE=3 SV=1
          Length = 270

 Score = 43.1 bits (100), Expect = 0.007
 Identities = 23/42 (54%), Positives = 24/42 (57%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D VTSP GTTIAGI  LEKAGFR            R +EL K
Sbjct: 229 DRVTSPGGTTIAGIAQLEKAGFRSALIEAVKAATWRSQELGK 270


>tr|Q3M924|Q3M924_ANAVT Pyrroline-5-carboxylate reductase
           OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937)
           GN=Ava_2901 PE=3 SV=1
          Length = 270

 Score = 43.1 bits (100), Expect = 0.007
 Identities = 23/42 (54%), Positives = 24/42 (57%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D VTSP GTTIAGI  LEKAGFR            R +EL K
Sbjct: 229 DRVTSPGGTTIAGIAQLEKAGFRSALIEAVKAATWRSQELGK 270


>tr|B2J1K7|B2J1K7_NOSP7 Pyrroline-5-carboxylate reductase OS=Nostoc
           punctiforme (strain ATCC 29133 / PCC 73102)
           GN=Npun_R1697 PE=4 SV=1
          Length = 272

 Score = 43.1 bits (100), Expect = 0.007
 Identities = 22/42 (52%), Positives = 25/42 (59%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELSK 127
           D VTSP GTTIAGI  LE+AGFR           +R +EL K
Sbjct: 231 DRVTSPGGTTIAGIAKLEQAGFRSALIEAVKAATERSQELGK 272


>tr|Q4TZJ2|Q4TZJ2_MAIZE Pyrroline-5-carboxylate reductase OS=Zea
           mays GN=p5cr PE=2 SV=1
          Length = 287

 Score = 43.1 bits (100), Expect = 0.007
 Identities = 23/41 (56%), Positives = 25/41 (60%)
 Frame = +2

Query: 2   DEVTSPAGTTIAGIRALEKAGFRXXXXXXXXXXXKRGEELS 124
           D+VTSPAGTTIAGI+ LEK  FR           KR  ELS
Sbjct: 247 DQVTSPAGTTIAGIQELEKGAFRGTLISAVVAAAKRCRELS 287