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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK950762
Clone id TST38A01NGRL0009_I20
Library
Length 670
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0009_I20. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
CTAAGACGATCTGTGCATCCTCATCCCTGATGCCTCCATTCTCATGCAACTTGCTCCCTC
TTCAAGCATCTTCGGCCTACCCCTTAGTCACGTGCGGACGGGGGGACAAGCGCACTGCCA
AGGGGAAGCGTTTCTTGGGCTCTTACGGCAATTCAAGACCAAGAAAGCCCAACAAGGGTA
GAGGCTTGCCAGAGGTGCCCTTACCACCTCGACCACCAAGGAAGGATCCATTGGATGATG
GTGAGTTTGTGGAGATCATTATAGATGACAATCTTTTCAAGAAGAAAGTTTAAGCGGGGC
ACCTCTTAAGATCGTTACATGGTTCGCTTCCTCATCAAGGAATTCTTGTGCGAGTTAAGT
CTGCTGAAAAACACGTATTGACGAAGGGGCATTTTGAAAAAAGGTATTATTACTTCTCTT
TGGCCAGCTAACATCAGCTGAAGATAGCCTTAAACTGATCGCTCATTTGCAAGATCACAT
GTAATGTGGTATTTCTTTTCTTTGATGTACCCCTGATTACATCAACATCTTGTGTATTTC
TTAGTTGAGCGTTTCTTGCAACCTTTGTTCCATATTACCAAGTGTCATTCAAGCTTTATG
TAAGCAAAACTGTCAATGCTATGAATGATGTGTCCAACAAAAATGCCAAAAAAAAAAAAA
AAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id O80439
Definition sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic OS=Arabidopsis thaliana
Align length 75
Score (bit) 58.9
E-value 2.0e-08
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950762|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0009_I20, 5'
         (647 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic OS...    59   2e-08
sp|P47910|RR31_SPIOL 30S ribosomal protein S31, chloroplastic OS...    56   2e-07
sp|P47909|RT31_ORYSJ 30S ribosomal protein S31, mitochondrial OS...    52   2e-06
sp|Q9SJU8|RT31_ARATH 30S ribosomal protein S31, mitochondrial OS...    48   5e-05
sp|P62612|RSHX_THETH 30S ribosomal protein Thx OS=Thermus thermo...    35   0.26 
sp|Q5SIH3|RSHX_THET8 30S ribosomal protein Thx OS=Thermus thermo...    35   0.26 
sp|P62613|RSHX_THET2 30S ribosomal protein Thx OS=Thermus thermo...    35   0.26 
sp|P62611|RSHX_THEAQ 30S ribosomal protein Thx OS=Thermus aquati...    35   0.26 
sp|Q7Z6G3|NECA2_HUMAN N-terminal EF-hand calcium-binding protein...    33   0.98 
sp|Q91ZP9|NECA2_MOUSE N-terminal EF-hand calcium-binding protein...    32   2.9  
sp|Q5R467|NECA1_PONAB N-terminal EF-hand calcium-binding protein...    31   6.4  
sp|Q8N987|NECA1_HUMAN N-terminal EF-hand calcium-binding protein...    31   6.4  
sp|Q9ESB5|NECA1_RAT N-terminal EF-hand calcium-binding protein 1...    30   8.3  
sp|Q8BG18|NECA1_MOUSE N-terminal EF-hand calcium-binding protein...    30   8.3  

>sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic
           OS=Arabidopsis thaliana GN=RPS31 PE=2 SV=1
          Length = 118

 Score = 58.9 bits (141), Expect = 2e-08
 Identities = 31/75 (41%), Positives = 42/75 (56%)
 Frame = +3

Query: 54  LPLQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXX 233
           L   +SS+ PLV CGRGD++TAKGKRF  S+GN+RPR  +K                   
Sbjct: 42  LSTSSSSSIPLVYCGRGDRKTAKGKRFNHSFGNARPRNKSKGRGPERVPVPPAPPRKDKF 101

Query: 234 XXXEFVEIIIDDNLF 278
              E ++I ID++LF
Sbjct: 102 ENDEKIKIDIDESLF 116


>sp|P47910|RR31_SPIOL 30S ribosomal protein S31, chloroplastic
           OS=Spinacia oleracea GN=RPS31 PE=1 SV=2
          Length = 101

 Score = 55.8 bits (133), Expect = 2e-07
 Identities = 26/58 (44%), Positives = 36/58 (62%)
 Frame = +3

Query: 3   KTICASSSLMPPFSCNLLPLQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           +++   S   PP       +  S A PL+ CGRGD++TAKGKRF  S+GN+RP+  NK
Sbjct: 24  QSVAGVSLSSPPTLSLSSSVSTSPALPLIYCGRGDRKTAKGKRFNHSFGNARPKNKNK 81


>sp|P47909|RT31_ORYSJ 30S ribosomal protein S31, mitochondrial
           OS=Oryza sativa subsp. japonica GN=Os09g0528100 PE=3
           SV=2
          Length = 82

 Score = 52.4 bits (124), Expect = 2e-06
 Identities = 21/30 (70%), Positives = 26/30 (86%)
 Frame = +3

Query: 87  VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           VTCGRGDK+T +GKRF GSYGN+RP++  K
Sbjct: 30  VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>sp|Q9SJU8|RT31_ARATH 30S ribosomal protein S31, mitochondrial
           OS=Arabidopsis thaliana GN=At2g21290 PE=2 SV=1
          Length = 98

 Score = 47.8 bits (112), Expect = 5e-05
 Identities = 24/40 (60%), Positives = 28/40 (70%), Gaps = 4/40 (10%)
 Frame = +3

Query: 60  LQASSAYPLVT----CGRGDKRTAKGKRFLGSYGNSRPRK 167
           L  + A P V+    CGRGDK+T KGKRF GSYGNSR +K
Sbjct: 33  LSPACAAPAVSEMDLCGRGDKKTKKGKRFKGSYGNSRGKK 72


>sp|P62612|RSHX_THETH 30S ribosomal protein Thx OS=Thermus
           thermophilus GN=rpsU PE=1 SV=2
          Length = 27

 Score = 35.4 bits (80), Expect = 0.26
 Identities = 14/24 (58%), Positives = 19/24 (79%)
 Frame = +3

Query: 96  GRGDKRTAKGKRFLGSYGNSRPRK 167
           G+GD+RT +GK + G+YG  RPRK
Sbjct: 2   GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|Q5SIH3|RSHX_THET8 30S ribosomal protein Thx OS=Thermus
           thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=rpsU
           PE=1 SV=3
          Length = 27

 Score = 35.4 bits (80), Expect = 0.26
 Identities = 14/24 (58%), Positives = 19/24 (79%)
 Frame = +3

Query: 96  GRGDKRTAKGKRFLGSYGNSRPRK 167
           G+GD+RT +GK + G+YG  RPRK
Sbjct: 2   GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|P62613|RSHX_THET2 30S ribosomal protein Thx OS=Thermus
           thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
           GN=rpsU PE=1 SV=2
          Length = 27

 Score = 35.4 bits (80), Expect = 0.26
 Identities = 14/24 (58%), Positives = 19/24 (79%)
 Frame = +3

Query: 96  GRGDKRTAKGKRFLGSYGNSRPRK 167
           G+GD+RT +GK + G+YG  RPRK
Sbjct: 2   GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|P62611|RSHX_THEAQ 30S ribosomal protein Thx OS=Thermus aquaticus
           GN=rpsU PE=1 SV=2
          Length = 27

 Score = 35.4 bits (80), Expect = 0.26
 Identities = 14/24 (58%), Positives = 19/24 (79%)
 Frame = +3

Query: 96  GRGDKRTAKGKRFLGSYGNSRPRK 167
           G+GD+RT +GK + G+YG  RPRK
Sbjct: 2   GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|Q7Z6G3|NECA2_HUMAN N-terminal EF-hand calcium-binding protein 2
           OS=Homo sapiens GN=NECAB2 PE=2 SV=1
          Length = 386

 Score = 33.5 bits (75), Expect = 0.98
 Identities = 17/67 (25%), Positives = 39/67 (58%), Gaps = 5/67 (7%)
 Frame = +1

Query: 157 DQESPTRVEACQRCPYHLDHQGRIHWMMVSLWRSL*MTI-----FSRRKFKRGTS*DRYM 321
           D ++   V+  + C Y +DH G    ++ SL  +L  ++     ++++ ++ G++ D+++
Sbjct: 107 DSDNTNHVDTKELCDYFVDHMGDYEDVLASL-ETLNHSVLKAMGYTKKVYEGGSNVDQFV 165

Query: 322 VRFLIKE 342
            RFL+KE
Sbjct: 166 TRFLLKE 172


>sp|Q91ZP9|NECA2_MOUSE N-terminal EF-hand calcium-binding protein 2
           OS=Mus musculus GN=Necab2 PE=1 SV=1
          Length = 389

 Score = 32.0 bits (71), Expect = 2.9
 Identities = 16/67 (23%), Positives = 39/67 (58%), Gaps = 5/67 (7%)
 Frame = +1

Query: 157 DQESPTRVEACQRCPYHLDHQGRIHWMMVSLWRSL*MTI-----FSRRKFKRGTS*DRYM 321
           D ++   V+  + C Y ++H G    ++ SL  +L  ++     ++++ ++ G++ D+++
Sbjct: 110 DSDNTNHVDTKELCDYFVEHMGDYEDVLASL-ETLNHSVLKAMGYTKKVYEGGSNVDQFV 168

Query: 322 VRFLIKE 342
            RFL+KE
Sbjct: 169 TRFLLKE 175
tr_hit_id B8LNB1
Definition tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 67
Score (bit) 57.0
E-value 1.0e-06
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950762|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0009_I20, 5'
         (647 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea...    57   1e-06
tr|A9NT69|A9NT69_PICSI Putative uncharacterized protein OS=Picea...    57   1e-06
tr|A9NME7|A9NME7_PICSI Putative uncharacterized protein OS=Picea...    57   1e-06
tr|B6UIF3|B6UIF3_MAIZE 30S ribosomal protein S31 OS=Zea mays PE=...    55   3e-06
tr|A5C1F7|A5C1F7_VITVI Putative uncharacterized protein OS=Vitis...    55   3e-06
tr|A7PP27|A7PP27_VITVI Chromosome chr8 scaffold_23, whole genome...    55   4e-06
tr|A9TLI9|A9TLI9_PHYPA Predicted protein OS=Physcomitrella paten...    55   5e-06
tr|Q0J066|Q0J066_ORYSJ Os09g0528100 protein OS=Oryza sativa subs...    52   2e-05
tr|A2Z3B9|A2Z3B9_ORYSI Putative uncharacterized protein OS=Oryza...    52   2e-05
tr|A7P7Y7|A7P7Y7_VITVI Chromosome chr3 scaffold_8, whole genome ...    52   3e-05
tr|A5AQK5|A5AQK5_VITVI Putative uncharacterized protein OS=Vitis...    52   3e-05
tr|A9RUK9|A9RUK9_PHYPA Predicted protein (Fragment) OS=Physcomit...    52   4e-05
tr|B6T2D1|B6T2D1_MAIZE PSRP4 OS=Zea mays PE=2 SV=1                     48   6e-04
tr|B6SR89|B6SR89_MAIZE PSRP4 OS=Zea mays PE=2 SV=1                     48   6e-04
tr|B4FP06|B4FP06_MAIZE Putative uncharacterized protein OS=Zea m...    48   6e-04
tr|Q60EZ7|Q60EZ7_ORYSJ Os05g0186000 protein OS=Oryza sativa subs...    47   8e-04
tr|B8AYV7|B8AYV7_ORYSI Putative uncharacterized protein OS=Oryza...    47   8e-04
tr|B6TPC8|B6TPC8_MAIZE PSRP4 OS=Zea mays PE=2 SV=1                     47   0.001
tr|A2A0P9|A2A0P9_9SPHI Putative uncharacterized protein OS=Micro...    45   0.005
tr|A9SJ69|A9SJ69_PHYPA Predicted protein OS=Physcomitrella paten...    45   0.005
tr|A7M6H4|A7M6H4_MALDO Putative uncharacterized protein (Fragmen...    43   0.019
tr|Q87AX9|Q87AX9_XYLFT Putative uncharacterized protein OS=Xylel...    40   0.093
tr|B2I8A5|B2I8A5_XYLF2 Putative uncharacterized protein OS=Xylel...    40   0.093
tr|B0U4F4|B0U4F4_XYLFM Putative uncharacterized protein OS=Xylel...    40   0.093
tr|Q3RCE8|Q3RCE8_XYLFA Putative uncharacterized protein OS=Xylel...    40   0.093
tr|Q3R1Z9|Q3R1Z9_XYLFA Putative uncharacterized protein OS=Xylel...    40   0.093
tr|Q9PGC0|Q9PGC0_XYLFA Putative uncharacterized protein OS=Xylel...    39   0.27 
tr|Q5H2N3|Q5H2N3_XANOR Putative uncharacterized protein OS=Xanth...    39   0.27 
tr|Q2P5K0|Q2P5K0_XANOM Putative uncharacterized protein XOO1422 ...    39   0.27 
tr|B2SSG3|B2SSG3_XANOP Putative uncharacterized protein OS=Xanth...    39   0.27 

>tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=4 SV=1
          Length = 112

 Score = 57.0 bits (136), Expect = 1e-06
 Identities = 30/67 (44%), Positives = 35/67 (52%)
 Frame = +3

Query: 78  YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 257
           YP V CGRGDKRTAKGKRF  S+GN+RPR   K                      E +EI
Sbjct: 45  YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 258 IIDDNLF 278
            ID++ F
Sbjct: 105 EIDESFF 111


>tr|A9NT69|A9NT69_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=4 SV=1
          Length = 112

 Score = 57.0 bits (136), Expect = 1e-06
 Identities = 30/67 (44%), Positives = 35/67 (52%)
 Frame = +3

Query: 78  YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 257
           YP V CGRGDKRTAKGKRF  S+GN+RPR   K                      E +EI
Sbjct: 45  YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 258 IIDDNLF 278
            ID++ F
Sbjct: 105 EIDESFF 111


>tr|A9NME7|A9NME7_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=4 SV=1
          Length = 112

 Score = 57.0 bits (136), Expect = 1e-06
 Identities = 30/67 (44%), Positives = 35/67 (52%)
 Frame = +3

Query: 78  YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 257
           YP V CGRGDKRTAKGKRF  S+GN+RPR   K                      E +EI
Sbjct: 45  YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 258 IIDDNLF 278
            ID++ F
Sbjct: 105 EIDESFF 111


>tr|B6UIF3|B6UIF3_MAIZE 30S ribosomal protein S31 OS=Zea mays PE=4
           SV=1
          Length = 88

 Score = 55.5 bits (132), Expect = 3e-06
 Identities = 24/39 (61%), Positives = 30/39 (76%)
 Frame = +3

Query: 60  LQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           L A+S    VTCGRGDK+T +GKRF GSYGN+RP++  K
Sbjct: 27  LAAASTAEAVTCGRGDKKTKRGKRFKGSYGNARPKREKK 65


>tr|A5C1F7|A5C1F7_VITVI Putative uncharacterized protein OS=Vitis
           vinifera GN=VITISV_030951 PE=4 SV=1
          Length = 117

 Score = 55.5 bits (132), Expect = 3e-06
 Identities = 34/90 (37%), Positives = 43/90 (47%), Gaps = 3/90 (3%)
 Frame = +3

Query: 18  SSSLMPPFSCNLLPLQASSA---YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXX 188
           S +L   F    + L  S+     P V CGRGDK+TAKGKRF  S+GN+RPR   K    
Sbjct: 27  SETLAVSFDTRFVSLSISATSPPIPSVYCGRGDKKTAKGKRFNHSFGNARPRNKKKGRGP 86

Query: 189 XXXXXXXXXXXXXXXXXXEFVEIIIDDNLF 278
                             E V+I ID++LF
Sbjct: 87  PRAPIPPSPPKKDRFEDDEVVKIEIDESLF 116


>tr|A7PP27|A7PP27_VITVI Chromosome chr8 scaffold_23, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00021486001
           PE=4 SV=1
          Length = 118

 Score = 55.1 bits (131), Expect = 4e-06
 Identities = 34/91 (37%), Positives = 43/91 (47%), Gaps = 4/91 (4%)
 Frame = +3

Query: 18  SSSLMPPFSCNLLPLQASSA----YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXX 185
           S +L   F    + L  S+      P V CGRGDK+TAKGKRF  S+GN+RPR   K   
Sbjct: 27  SETLAVSFDTRFVSLSISATSPPPIPSVYCGRGDKKTAKGKRFNHSFGNARPRNKKKGRG 86

Query: 186 XXXXXXXXXXXXXXXXXXXEFVEIIIDDNLF 278
                              E V+I ID++LF
Sbjct: 87  PPRAPIPPSPPKKDRFEDDEVVKIEIDESLF 117


>tr|A9TLI9|A9TLI9_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_196235 PE=4 SV=1
          Length = 109

 Score = 54.7 bits (130), Expect = 5e-06
 Identities = 35/102 (34%), Positives = 51/102 (50%), Gaps = 8/102 (7%)
 Frame = +3

Query: 6   TICASSSL----MPPFSCNLLPLQASSA----YPLVTCGRGDKRTAKGKRFLGSYGNSRP 161
           ++C SSS     +P  S   +   +SS+     P + CGRGDKRTAKGKR  GS+GN RP
Sbjct: 10  SLCCSSSTFFKALPMPSAAAVSSSSSSSSSVSLPAIVCGRGDKRTAKGKRARGSFGNCRP 69

Query: 162 RKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEIIIDDNLFKKK 287
           +  +K                      EF+ + ID++LF ++
Sbjct: 70  KDSSK--GTGLAITPLPPRTKKEVEDTEFINVEIDESLFNRQ 109


>tr|Q0J066|Q0J066_ORYSJ Os09g0528100 protein OS=Oryza sativa subsp.
           japonica GN=Os09g0528100 PE=4 SV=1
          Length = 82

 Score = 52.4 bits (124), Expect = 2e-05
 Identities = 21/30 (70%), Positives = 26/30 (86%)
 Frame = +3

Query: 87  VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           VTCGRGDK+T +GKRF GSYGN+RP++  K
Sbjct: 30  VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>tr|A2Z3B9|A2Z3B9_ORYSI Putative uncharacterized protein OS=Oryza
           sativa subsp. indica GN=OsI_32120 PE=4 SV=1
          Length = 82

 Score = 52.4 bits (124), Expect = 2e-05
 Identities = 21/30 (70%), Positives = 26/30 (86%)
 Frame = +3

Query: 87  VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           VTCGRGDK+T +GKRF GSYGN+RP++  K
Sbjct: 30  VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>tr|A7P7Y7|A7P7Y7_VITVI Chromosome chr3 scaffold_8, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00035968001
           PE=4 SV=1
          Length = 88

 Score = 52.0 bits (123), Expect = 3e-05
 Identities = 21/30 (70%), Positives = 25/30 (83%)
 Frame = +3

Query: 87  VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 176
           + CGRGDK+T KGKRF GSYGN+RP+K  K
Sbjct: 36  ILCGRGDKKTKKGKRFKGSYGNARPKKEKK 65