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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK950821
Clone id TST38A01NGRL0009_L14
Library
Length 714
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0009_L14. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID -
Sequence 
CAGAGCCAACGGGTGTTTTGAATGGAAGATCTTCACAATCGGTAAAAGGATACACGGAAG
TCAACTCAGGATCCTTTGCTAGAATCAATGGAGCCAGCAGAATAGCAGAAGCCGCTAAAT
TACCGGAAGCTTTGCCTTCCACTTCAAGGTTATCATTGAAACAGGCACATCTTCGAAAAT
CCCACTCAAATTTTACTGTTCCGCAGCCCTTTGCACTTGCAACTGATAAAAGAGCTTCCT
TAGGAGGGCAACCACACTCTTCAAGTAGAGTTTACGTGTCTTCCAGGTCTTTCAGCTCTC
AGATAATTCGAGAAGGTGAAAAGAAAGCTAATGACGGCAAAGGAGATGACCTAAAATTTC
AAGAGTCTGTTAAGCATGAAGAGACCAAAGCACAGTTGAAGGCAAGTGCCAATACGTTCA
ACTTCAATAGTGACGTCCGTGCTGAACGAAGGAAAGAGTTCAATTCGAAAATGGAGGAGC
GTCTAACAGCAAAGGAGGCAGAGAAGACTCAAGCGCAAGCCAAAACAAAGAAGGTTATAC
AAGCTGAAATAAAGCAGTTTCGGAAGAGTCTTACTTTCAAAGCTACTCCTATGCCGTCAT
TCTATCAAGATGCGACTCCACCAAAACTAGAGATCAAGAAAATACCTCCNACAAGGGCCA
AGTCACCAAAGCTAGGTAGAAGGAACAGTGCTACAGGGGCTCATGCTGGTCATT
■■Homology search results ■■ -
sp_hit_id P20237
Definition sp|P20237|GBRA4_BOVIN Gamma-aminobutyric acid receptor subunit alpha-4 OS=Bos taurus
Align length 43
Score (bit) 33.1
E-value 1.5
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950821|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0009_L14, 5'
         (714 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P20237|GBRA4_BOVIN Gamma-aminobutyric acid receptor subunit a...    33   1.5  
sp|P36188|TNNI_DROME Troponin I OS=Drosophila melanogaster GN=wu...    32   2.6  
sp|Q28969|NOS3_PIG Nitric oxide synthase, endothelial OS=Sus scr...    31   5.8  

>sp|P20237|GBRA4_BOVIN Gamma-aminobutyric acid receptor subunit
           alpha-4 OS=Bos taurus GN=GABRA4 PE=2 SV=2
          Length = 555

 Score = 33.1 bits (74), Expect = 1.5
 Identities = 16/43 (37%), Positives = 23/43 (53%)
 Frame = +3

Query: 15  VLNGRSSQSVKGYTEVNSGSFARINGASRIAEAAKLPEALPST 143
           V+ G S  + + Y   +   F+R N A  I+ A  +P ALPST
Sbjct: 416 VVQGSSEATPQSYLASSPNPFSRANAAETISAARAIPSALPST 458


>sp|P36188|TNNI_DROME Troponin I OS=Drosophila melanogaster GN=wupA
           PE=1 SV=3
          Length = 269

 Score = 32.3 bits (72), Expect = 2.6
 Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 2/61 (3%)
 Frame = +3

Query: 306 IREGEKKANDGKGDDLK--FQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEE 479
           I +   + ND +G  +K   ++  K+E   A+L+  A  FNF + ++  ++KEF  + EE
Sbjct: 185 INDLNAQVNDLRGKFVKPALKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEE 244

Query: 480 R 482
           +
Sbjct: 245 K 245


>sp|Q28969|NOS3_PIG Nitric oxide synthase, endothelial OS=Sus scrofa
           GN=NOS3 PE=1 SV=4
          Length = 1205

 Score = 31.2 bits (69), Expect = 5.8
 Identities = 18/52 (34%), Positives = 25/52 (48%)
 Frame = +3

Query: 315 GEKKANDGKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSK 470
           GE+    G+GD+L  QE       +A  +AS  TF    D +A  R  F+ K
Sbjct: 678 GERLLQLGQGDELCGQEEAFRGWAQAAFQASCETFCVGEDAKAAARDIFSPK 729
tr_hit_id A9TU86
Definition tr|A9TU86|A9TU86_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 260
Score (bit) 128.0
E-value 4.0e-28
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK950821|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0009_L14, 5'
         (714 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A9TU86|A9TU86_PHYPA Predicted protein OS=Physcomitrella paten...   128   4e-28
tr|Q7XYW3|Q7XYW3_BRANA Seed specific protein Bn15D14A (Fragment)...   113   1e-23
tr|A9P9R6|A9P9R6_POPTR Putative uncharacterized protein OS=Popul...   113   1e-23
tr|Q94C48|Q94C48_ARATH Putative uncharacterized protein At4g3233...   108   3e-22
tr|A7P5T9|A7P5T9_VITVI Chromosome chr4 scaffold_6, whole genome ...   108   3e-22
tr|Q761Z6|Q761Z6_ORYSJ BRI1-KD interacting protein 118 (Fragment...   108   4e-22
tr|Q6Z797|Q6Z797_ORYSJ Putative BRI1-KD interacting protein OS=O...   108   4e-22
tr|Q0E303|Q0E303_ORYSJ Os02g0200800 protein (Fragment) OS=Oryza ...   108   4e-22
tr|A3A489|A3A489_ORYSJ Putative uncharacterized protein OS=Oryza...   108   4e-22
tr|A2X244|A2X244_ORYSI Putative uncharacterized protein OS=Oryza...   107   6e-22
tr|Q3E9S8|Q3E9S8_ARATH Uncharacterized protein At4g32330.2 OS=Ar...   105   2e-21
tr|B7ZXM3|B7ZXM3_MAIZE Putative uncharacterized protein OS=Zea m...   102   2e-20
tr|B6U8B2|B6U8B2_MAIZE WDL1 OS=Zea mays PE=2 SV=1                     102   2e-20
tr|B4FBB6|B4FBB6_MAIZE Putative uncharacterized protein OS=Zea m...   102   2e-20
tr|A9PG41|A9PG41_POPTR Putative uncharacterized protein OS=Popul...   102   3e-20
tr|B6TK06|B6TK06_MAIZE Lymphoid organ expressed yellow head viru...   101   4e-20
tr|Q9LS82|Q9LS82_ARATH Gb|AAF04893.1 OS=Arabidopsis thaliana PE=...   101   5e-20
tr|Q84WL6|Q84WL6_ARATH At3g23090 (Putative uncharacterized prote...   101   5e-20
tr|B4FRD7|B4FRD7_MAIZE Putative uncharacterized protein OS=Zea m...   100   1e-19
tr|A9SSX1|A9SSX1_PHYPA Predicted protein (Fragment) OS=Physcomit...   100   1e-19
tr|Q9SKK1|Q9SKK1_ARATH Putative uncharacterized protein At2g2548...    99   2e-19
tr|A9SIL1|A9SIL1_PHYPA Predicted protein (Fragment) OS=Physcomit...    99   2e-19
tr|Q2R2B9|Q2R2B9_ORYSJ Os11g0571900 protein OS=Oryza sativa subs...    98   5e-19
tr|Q0WSZ8|Q0WSZ8_ARATH Putative uncharacterized protein At2g2548...    98   5e-19
tr|B8BL54|B8BL54_ORYSI Putative uncharacterized protein OS=Oryza...    98   5e-19
tr|B6T3N0|B6T3N0_MAIZE Seed specific protein Bn15D14A OS=Zea may...    98   5e-19
tr|Q9SJ62|Q9SJ62_ARATH Expressed protein (Putative uncharacteriz...    97   1e-18
tr|A7QMQ2|A7QMQ2_VITVI Chromosome undetermined scaffold_127, who...    97   1e-18
tr|A9TNQ1|A9TNQ1_PHYPA Predicted protein (Fragment) OS=Physcomit...    96   2e-18
tr|Q69Q42|Q69Q42_ORYSJ Os06g0606800 protein OS=Oryza sativa subs...    96   3e-18

>tr|A9TU86|A9TU86_PHYPA Predicted protein OS=Physcomitrella patens
            subsp. patens GN=PHYPADRAFT_97346 PE=4 SV=1
          Length = 775

 Score =  128 bits (321), Expect = 4e-28
 Identities = 88/260 (33%), Positives = 132/260 (50%), Gaps = 27/260 (10%)
 Frame = +3

Query: 12   GVLNGRSSQSVKGYTEVNSGSFARINGASRIAEAAKLPEALPSTSRLSLKQAHLRKSHSN 191
            G++ G+ + SV    +    S   +  A   +   KL       +  S+ ++ +R S + 
Sbjct: 325  GIVTGKKAPSVAELGKPAKPSTTSVQPALDGSLKTKLAAMEEGETSDSVPKSSVRGSRAK 384

Query: 192  FTVPQPFALATDKRASLGGQPHXXXXXXXXXXXXXX-----------------------Q 302
             T+PQPF LAT+KRASLG +P                                      +
Sbjct: 385  LTIPQPFRLATEKRASLGVRPADAETTRPRATSNVVKKSHPVPTTKTAGATGRIMAFPTE 444

Query: 303  IIREGEKKANDGKGD-DLKFQESVKHEETKAQLKA-SANTFNFNSDVRAERRKEFNSKME 476
            I +E ++  +  + D + KF+E    E T  ++K+ + + F+F SD RAE+R+EF SK+E
Sbjct: 445  ISKEDQEALDTSEKDKEAKFEE----ELTVLRIKSGNPSGFSFKSDERAEKRREFYSKLE 500

Query: 477  ERLXXXXXXXXXXXXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTR 656
            E++                 ++ ++KQ RKSLTFKATP+PSFYQ++ PPK+E+KKIPPTR
Sbjct: 501  EKMKAKEEEMSQSQAKSQEELENKMKQLRKSLTFKATPLPSFYQESGPPKVEVKKIPPTR 560

Query: 657  AKSPKL--GRRNSATGAHAG 710
             KSPKL   RR S  G H G
Sbjct: 561  PKSPKLTTARRGSPRGEHDG 580


>tr|Q7XYW3|Q7XYW3_BRANA Seed specific protein Bn15D14A (Fragment)
           OS=Brassica napus PE=2 SV=1
          Length = 267

 Score =  113 bits (283), Expect = 1e-23
 Identities = 78/208 (37%), Positives = 103/208 (49%), Gaps = 11/208 (5%)
 Frame = +3

Query: 108 EAAKLPEALPSTSRLSLKQAHLRK-----SHSNFTVPQPFALATDKRASLGG-QPHXXXX 269
           E A L +   S ++  L   H+ K     +    TVPQPFALAT KRAS    + H    
Sbjct: 3   EIATLRKDAKSVNKSVLHAKHVSKPGRGSNKIRNTVPQPFALATAKRASSATRESHEVAD 62

Query: 270 XXXXXXXXXXQIIREGEKKANDGKGDDLKFQESVKHEETKAQLKASANTF-----NFNSD 434
                     ++ R+  +  N    D+ +   SV    T A   A + T      +F S 
Sbjct: 63  MYSKIQKQVIKVPRKPLQPKNKKLSDEEEDSCSVASYATSAAKSAKSRTIVTAAPSFRST 122

Query: 435 VRAERRKEFNSKMEERLXXXXXXXXXXXXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDA 614
            RAE+RKEF +K+EE+                    A ++Q RKSL FKA PMP+FY + 
Sbjct: 123 ERAEKRKEFYTKLEEKHQAMEAEKTQSEARNKEEKDAALRQLRKSLMFKANPMPNFYHEG 182

Query: 615 TPPKLEIKKIPPTRAKSPKLGRRNSATG 698
            PPK+E+KK PPTRAKSPKLGRRN+  G
Sbjct: 183 PPPKVELKKPPPTRAKSPKLGRRNTKEG 210


>tr|A9P9R6|A9P9R6_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 404

 Score =  113 bits (283), Expect = 1e-23
 Identities = 76/206 (36%), Positives = 104/206 (50%), Gaps = 20/206 (9%)
 Frame = +3

Query: 138 STSRLSLKQAHLRKSHSNFTVPQPFALATDKRASLGGQP-------HXXXXXXXXXXXXX 296
           S+++ + K A    + +  TVPQPFALAT+KRASLG +P       +             
Sbjct: 121 SSTKHASKPAPAGLARTKHTVPQPFALATEKRASLGMRPSGEPDITNGLNKSFKANNALR 180

Query: 297 XQIIREGE------------KKANDGKGDDLKFQESVKHEETKAQLKASANTFN-FNSDV 437
              I++ +             K +  + D+     S       A+ K +A     F  + 
Sbjct: 181 PNPIKQNQPLSVSRKPLQPNNKKHPDEEDNCSVTSSTTASARPAESKPAAVAAPVFRCNE 240

Query: 438 RAERRKEFNSKMEERLXXXXXXXXXXXXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDAT 617
           RAE+RKEF SK+EE+                   +A IKQ RKSL FKA+PMPSFY +  
Sbjct: 241 RAEKRKEFYSKLEEKHLALEAEKTQSEARTKEEKEAAIKQLRKSLMFKASPMPSFYHEGP 300

Query: 618 PPKLEIKKIPPTRAKSPKLGRRNSAT 695
           PPK+E+KK+PPTRAKSPKLGRR S +
Sbjct: 301 PPKVELKKLPPTRAKSPKLGRRKSCS 326


>tr|Q94C48|Q94C48_ARATH Putative uncharacterized protein At4g32330
           OS=Arabidopsis thaliana GN=At4g32330 PE=2 SV=1
          Length = 437

 Score =  108 bits (271), Expect = 3e-22
 Identities = 58/130 (44%), Positives = 79/130 (60%), Gaps = 2/130 (1%)
 Frame = +3

Query: 318 EKKANDGKGDDLKFQESVKHEETKAQLKASANT--FNFNSDVRAERRKEFNSKMEERLXX 491
           +K+A++   DD +   S K ++ K +   +     F+F  D RAE+RKEF  K+EE+   
Sbjct: 172 KKQAHETSEDDTQSSNSPKADDGKPRKVGALPNYGFSFKCDQRAEKRKEFYVKLEEKTHA 231

Query: 492 XXXXXXXXXXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPK 671
                           +AE++  RKSL FKATPMPSFYQ+  PPK E+KKIPPTR KSPK
Sbjct: 232 KEEEINSMQAKSKETQEAELRMLRKSLNFKATPMPSFYQEPQPPKTELKKIPPTRPKSPK 291

Query: 672 LGRRNSATGA 701
           LGR+ +A+GA
Sbjct: 292 LGRKKTASGA 301


>tr|A7P5T9|A7P5T9_VITVI Chromosome chr4 scaffold_6, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00032657001
           PE=4 SV=1
          Length = 431

 Score =  108 bits (270), Expect = 3e-22
 Identities = 56/107 (52%), Positives = 73/107 (68%), Gaps = 2/107 (1%)
 Frame = +3

Query: 378 EETKAQLKASANTFNFN--SDVRAERRKEFNSKMEERLXXXXXXXXXXXXXXXXVIQAEI 551
           E+TK +  A+  ++NF+   D RAE+R+EF +K+EE+                   +AEI
Sbjct: 219 EDTKPRRVAALPSYNFSFRCDERAEKRREFYTKLEEKTHAKEIERTNLQAKSKETQEAEI 278

Query: 552 KQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGRRNSA 692
           K  RKSLTFKATPMPSFYQ+  PPK+E+KKIPPTRAKSPKLGR+ S+
Sbjct: 279 KMLRKSLTFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSS 325


>tr|Q761Z6|Q761Z6_ORYSJ BRI1-KD interacting protein 118 (Fragment)
           OS=Oryza sativa subsp. japonica GN=bip118 PE=2 SV=1
          Length = 334

 Score =  108 bits (269), Expect = 4e-22
 Identities = 59/122 (48%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
 Frame = +3

Query: 336 GKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEERLXXXXXXXXXX 515
           G+   +K  +    E  KA     A  F+F  D RAE+RKEF SK+EE++          
Sbjct: 102 GQNSSVKKDDESNPESRKAG-GTPAYGFSFKCDERAEKRKEFYSKLEEKIHAREMEISNL 160

Query: 516 XXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGR-RNSA 692
                   +AE+K  RKSL FKATPMPSFYQ+ TPPK+E+KKIPPTRA+SPKLGR +N +
Sbjct: 161 QAKSKETEEAELKMLRKSLNFKATPMPSFYQEPTPPKVELKKIPPTRARSPKLGRSKNKS 220

Query: 693 TG 698
            G
Sbjct: 221 AG 222


>tr|Q6Z797|Q6Z797_ORYSJ Putative BRI1-KD interacting protein
           OS=Oryza sativa subsp. japonica GN=P0419A09.11 PE=4 SV=1
          Length = 395

 Score =  108 bits (269), Expect = 4e-22
 Identities = 59/122 (48%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
 Frame = +3

Query: 336 GKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEERLXXXXXXXXXX 515
           G+   +K  +    E  KA     A  F+F  D RAE+RKEF SK+EE++          
Sbjct: 163 GQNSSVKKDDESNPESRKAG-GTPAYGFSFKCDERAEKRKEFYSKLEEKIHAREMEISNL 221

Query: 516 XXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGR-RNSA 692
                   +AE+K  RKSL FKATPMPSFYQ+ TPPK+E+KKIPPTRA+SPKLGR +N +
Sbjct: 222 QAKSKETEEAELKMLRKSLNFKATPMPSFYQEPTPPKVELKKIPPTRARSPKLGRSKNKS 281

Query: 693 TG 698
            G
Sbjct: 282 AG 283


>tr|Q0E303|Q0E303_ORYSJ Os02g0200800 protein (Fragment) OS=Oryza
           sativa subsp. japonica GN=Os02g0200800 PE=4 SV=1
          Length = 334

 Score =  108 bits (269), Expect = 4e-22
 Identities = 59/122 (48%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
 Frame = +3

Query: 336 GKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEERLXXXXXXXXXX 515
           G+   +K  +    E  KA     A  F+F  D RAE+RKEF SK+EE++          
Sbjct: 102 GQNSSVKKDDESNPESRKAG-GTPAYGFSFKCDERAEKRKEFYSKLEEKIHAREMEISNL 160

Query: 516 XXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGR-RNSA 692
                   +AE+K  RKSL FKATPMPSFYQ+ TPPK+E+KKIPPTRA+SPKLGR +N +
Sbjct: 161 QAKSKETEEAELKMLRKSLNFKATPMPSFYQEPTPPKVELKKIPPTRARSPKLGRSKNKS 220

Query: 693 TG 698
            G
Sbjct: 221 AG 222


>tr|A3A489|A3A489_ORYSJ Putative uncharacterized protein OS=Oryza
           sativa subsp. japonica GN=OsJ_005611 PE=4 SV=1
          Length = 410

 Score =  108 bits (269), Expect = 4e-22
 Identities = 59/122 (48%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
 Frame = +3

Query: 336 GKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEERLXXXXXXXXXX 515
           G+   +K  +    E  KA     A  F+F  D RAE+RKEF SK+EE++          
Sbjct: 163 GQNSSVKKDDESNPESRKAG-GTPAYGFSFKCDERAEKRKEFYSKLEEKIHAREMEISNL 221

Query: 516 XXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGR-RNSA 692
                   +AE+K  RKSL FKATPMPSFYQ+ TPPK+E+KKIPPTRA+SPKLGR +N +
Sbjct: 222 QAKSKETEEAELKMLRKSLNFKATPMPSFYQEPTPPKVELKKIPPTRARSPKLGRSKNKS 281

Query: 693 TG 698
            G
Sbjct: 282 AG 283


>tr|A2X244|A2X244_ORYSI Putative uncharacterized protein OS=Oryza
           sativa subsp. indica GN=OsI_06272 PE=4 SV=1
          Length = 395

 Score =  107 bits (268), Expect = 6e-22
 Identities = 59/122 (48%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
 Frame = +3

Query: 336 GKGDDLKFQESVKHEETKAQLKASANTFNFNSDVRAERRKEFNSKMEERLXXXXXXXXXX 515
           G+   +K  +    E  KA     A  F+F  D RAE+RKEF SK+EE++          
Sbjct: 163 GQNSSVKKDDESNPESRKAG-GIPAYGFSFKCDERAEKRKEFYSKLEEKIHAREMEISNL 221

Query: 516 XXXXXXVIQAEIKQFRKSLTFKATPMPSFYQDATPPKLEIKKIPPTRAKSPKLGR-RNSA 692
                   +AE+K  RKSL FKATPMPSFYQ+ TPPK+E+KKIPPTRA+SPKLGR +N +
Sbjct: 222 QAKSKETEEAELKMLRKSLNFKATPMPSFYQEPTPPKVELKKIPPTRARSPKLGRSKNKS 281

Query: 693 TG 698
            G
Sbjct: 282 AG 283