DK951078
Clone id TST38A01NGRL0010_G23
Library
Length 518
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_G23. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence
CCACAAGCAATGGCAGCAGCGTCGTCGGCATCAGTTATGGCCTCCTCTGTCGCGCCGCCA
GCGATGATGGGTTTCACTAAGACGATCTGTGCATCCTCATCCCTGATGCCTCCATTCTCA
TGCAACTTGCTCCCTCTTCAAGCATCTTCGGCCTACCCCTTAGTCACGTGCGGACGGGGG
GACAAGCGCACTGCCAAGGGGAAGCGTTTCTTGGGCTCTTACGGCAATTCAAGACCAAGA
AAGCCCAACAAGGGTAGAGGCTTGCCAGAGGTGCCCTTACCACCTCGACCACCAAGGAAG
GATCCATTGGATGATGGTGAGTTTGTGGAGATCATTATAGATGACAATCTTTTCAAGAAG
AAAGTTTAAGCGGGGCACCTCTTAAGATCGTTACATGGTTCGCTTCCTCATCAAGGAATT
CTTGTGCGAGTTAAGTCTGCTGAAAAACACGTATTGACGAAGGGGCATTTTGAAAAAAGG
TATTATTACTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id O80439
Definition sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic OS=Arabidopsis thaliana
Align length 75
Score (bit) 58.9
E-value 1.0e-08
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK951078|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0010_G23, 5'
(492 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic OS... 59 1e-08
sp|P47910|RR31_SPIOL 30S ribosomal protein S31, chloroplastic OS... 56 8e-08
sp|P47909|RT31_ORYSJ 30S ribosomal protein S31, mitochondrial OS... 52 1e-06
sp|Q9SJU8|RT31_ARATH 30S ribosomal protein S31, mitochondrial OS... 48 3e-05
sp|P62612|RSHX_THETH 30S ribosomal protein Thx OS=Thermus thermo... 35 0.14
sp|Q5SIH3|RSHX_THET8 30S ribosomal protein Thx OS=Thermus thermo... 35 0.14
sp|P62613|RSHX_THET2 30S ribosomal protein Thx OS=Thermus thermo... 35 0.14
sp|P62611|RSHX_THEAQ 30S ribosomal protein Thx OS=Thermus aquati... 35 0.14
sp|Q7Z6G3|NECA2_HUMAN N-terminal EF-hand calcium-binding protein... 33 0.54
sp|Q91ZP9|NECA2_MOUSE N-terminal EF-hand calcium-binding protein... 32 1.6
sp|Q9ESB5|NECA1_RAT N-terminal EF-hand calcium-binding protein 1... 30 7.8
sp|Q5R467|NECA1_PONAB N-terminal EF-hand calcium-binding protein... 30 7.8
sp|Q8BG18|NECA1_MOUSE N-terminal EF-hand calcium-binding protein... 30 7.8
sp|Q8N987|NECA1_HUMAN N-terminal EF-hand calcium-binding protein... 30 7.8

>sp|O80439|RR31_ARATH 30S ribosomal protein S31, chloroplastic
OS=Arabidopsis thaliana GN=RPS31 PE=2 SV=1
Length = 118

Score = 58.9 bits (141), Expect = 1e-08
Identities = 31/75 (41%), Positives = 42/75 (56%)
Frame = +1

Query: 130 LPLQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXX 309
L +SS+ PLV CGRGD++TAKGKRF S+GN+RPR +K
Sbjct: 42 LSTSSSSSIPLVYCGRGDRKTAKGKRFNHSFGNARPRNKSKGRGPERVPVPPAPPRKDKF 101

Query: 310 XXXEFVEIIIDDNLF 354
E ++I ID++LF
Sbjct: 102 ENDEKIKIDIDESLF 116


>sp|P47910|RR31_SPIOL 30S ribosomal protein S31, chloroplastic
OS=Spinacia oleracea GN=RPS31 PE=1 SV=2
Length = 101

Score = 56.2 bits (134), Expect = 8e-08
Identities = 26/59 (44%), Positives = 37/59 (62%)
Frame = +1

Query: 76 TKTICASSSLMPPFSCNLLPLQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
++++ S PP + S A PL+ CGRGD++TAKGKRF S+GN+RP+ NK
Sbjct: 23 SQSVAGVSLSSPPTLSLSSSVSTSPALPLIYCGRGDRKTAKGKRFNHSFGNARPKNKNK 81


>sp|P47909|RT31_ORYSJ 30S ribosomal protein S31, mitochondrial
OS=Oryza sativa subsp. japonica GN=Os09g0528100 PE=3
SV=2
Length = 82

Score = 52.4 bits (124), Expect = 1e-06
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +1

Query: 163 VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
VTCGRGDK+T +GKRF GSYGN+RP++ K
Sbjct: 30 VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>sp|Q9SJU8|RT31_ARATH 30S ribosomal protein S31, mitochondrial
OS=Arabidopsis thaliana GN=At2g21290 PE=2 SV=1
Length = 98

Score = 47.8 bits (112), Expect = 3e-05
Identities = 24/40 (60%), Positives = 28/40 (70%), Gaps = 4/40 (10%)
Frame = +1

Query: 136 LQASSAYPLVT----CGRGDKRTAKGKRFLGSYGNSRPRK 243
L + A P V+ CGRGDK+T KGKRF GSYGNSR +K
Sbjct: 33 LSPACAAPAVSEMDLCGRGDKKTKKGKRFKGSYGNSRGKK 72


>sp|P62612|RSHX_THETH 30S ribosomal protein Thx OS=Thermus
thermophilus GN=rpsU PE=1 SV=2
Length = 27

Score = 35.4 bits (80), Expect = 0.14
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1

Query: 172 GRGDKRTAKGKRFLGSYGNSRPRK 243
G+GD+RT +GK + G+YG RPRK
Sbjct: 2 GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|Q5SIH3|RSHX_THET8 30S ribosomal protein Thx OS=Thermus
thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=rpsU
PE=1 SV=3
Length = 27

Score = 35.4 bits (80), Expect = 0.14
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1

Query: 172 GRGDKRTAKGKRFLGSYGNSRPRK 243
G+GD+RT +GK + G+YG RPRK
Sbjct: 2 GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|P62613|RSHX_THET2 30S ribosomal protein Thx OS=Thermus
thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
GN=rpsU PE=1 SV=2
Length = 27

Score = 35.4 bits (80), Expect = 0.14
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1

Query: 172 GRGDKRTAKGKRFLGSYGNSRPRK 243
G+GD+RT +GK + G+YG RPRK
Sbjct: 2 GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|P62611|RSHX_THEAQ 30S ribosomal protein Thx OS=Thermus aquaticus
GN=rpsU PE=1 SV=2
Length = 27

Score = 35.4 bits (80), Expect = 0.14
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1

Query: 172 GRGDKRTAKGKRFLGSYGNSRPRK 243
G+GD+RT +GK + G+YG RPRK
Sbjct: 2 GKGDRRTRRGKIWRGTYGKYRPRK 25


>sp|Q7Z6G3|NECA2_HUMAN N-terminal EF-hand calcium-binding protein 2
OS=Homo sapiens GN=NECAB2 PE=2 SV=1
Length = 386

Score = 33.5 bits (75), Expect = 0.54
Identities = 17/67 (25%), Positives = 39/67 (58%), Gaps = 5/67 (7%)
Frame = +2

Query: 233 DQESPTRVEACQRCPYHLDHQGRIHWMMVSLWRSL*MTI-----FSRRKFKRGTS*DRYM 397
D ++ V+ + C Y +DH G ++ SL +L ++ ++++ ++ G++ D+++
Sbjct: 107 DSDNTNHVDTKELCDYFVDHMGDYEDVLASL-ETLNHSVLKAMGYTKKVYEGGSNVDQFV 165

Query: 398 VRFLIKE 418
RFL+KE
Sbjct: 166 TRFLLKE 172


>sp|Q91ZP9|NECA2_MOUSE N-terminal EF-hand calcium-binding protein 2
OS=Mus musculus GN=Necab2 PE=1 SV=1
Length = 389

Score = 32.0 bits (71), Expect = 1.6
Identities = 16/67 (23%), Positives = 39/67 (58%), Gaps = 5/67 (7%)
Frame = +2

Query: 233 DQESPTRVEACQRCPYHLDHQGRIHWMMVSLWRSL*MTI-----FSRRKFKRGTS*DRYM 397
D ++ V+ + C Y ++H G ++ SL +L ++ ++++ ++ G++ D+++
Sbjct: 110 DSDNTNHVDTKELCDYFVEHMGDYEDVLASL-ETLNHSVLKAMGYTKKVYEGGSNVDQFV 168

Query: 398 VRFLIKE 418
RFL+KE
Sbjct: 169 TRFLLKE 175


tr_hit_id B8LNB1
Definition tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 67
Score (bit) 57.0
E-value 5.0e-07
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK951078|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0010_G23, 5'
(492 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea... 57 5e-07
tr|A9NT69|A9NT69_PICSI Putative uncharacterized protein OS=Picea... 57 5e-07
tr|A9NME7|A9NME7_PICSI Putative uncharacterized protein OS=Picea... 57 5e-07
tr|B6UIF3|B6UIF3_MAIZE 30S ribosomal protein S31 OS=Zea mays PE=... 55 1e-06
tr|A5C1F7|A5C1F7_VITVI Putative uncharacterized protein OS=Vitis... 55 1e-06
tr|A7PP27|A7PP27_VITVI Chromosome chr8 scaffold_23, whole genome... 55 2e-06
tr|A9TLI9|A9TLI9_PHYPA Predicted protein OS=Physcomitrella paten... 55 2e-06
tr|Q0J066|Q0J066_ORYSJ Os09g0528100 protein OS=Oryza sativa subs... 52 1e-05
tr|A2Z3B9|A2Z3B9_ORYSI Putative uncharacterized protein OS=Oryza... 52 1e-05
tr|A7P7Y7|A7P7Y7_VITVI Chromosome chr3 scaffold_8, whole genome ... 52 2e-05
tr|A5AQK5|A5AQK5_VITVI Putative uncharacterized protein OS=Vitis... 52 2e-05
tr|A9RUK9|A9RUK9_PHYPA Predicted protein (Fragment) OS=Physcomit... 52 2e-05
tr|B6T2D1|B6T2D1_MAIZE PSRP4 OS=Zea mays PE=2 SV=1 48 3e-04
tr|B6SR89|B6SR89_MAIZE PSRP4 OS=Zea mays PE=2 SV=1 48 3e-04
tr|B4FP06|B4FP06_MAIZE Putative uncharacterized protein OS=Zea m... 48 3e-04
tr|Q60EZ7|Q60EZ7_ORYSJ Os05g0186000 protein OS=Oryza sativa subs... 47 4e-04
tr|B8AYV7|B8AYV7_ORYSI Putative uncharacterized protein OS=Oryza... 47 4e-04
tr|A9SJ69|A9SJ69_PHYPA Predicted protein OS=Physcomitrella paten... 47 5e-04
tr|B6TPC8|B6TPC8_MAIZE PSRP4 OS=Zea mays PE=2 SV=1 47 6e-04
tr|A2A0P9|A2A0P9_9SPHI Putative uncharacterized protein OS=Micro... 45 0.002
tr|A7M6H4|A7M6H4_MALDO Putative uncharacterized protein (Fragmen... 43 0.009
tr|Q87AX9|Q87AX9_XYLFT Putative uncharacterized protein OS=Xylel... 40 0.046
tr|B2I8A5|B2I8A5_XYLF2 Putative uncharacterized protein OS=Xylel... 40 0.046
tr|B0U4F4|B0U4F4_XYLFM Putative uncharacterized protein OS=Xylel... 40 0.046
tr|Q3RCE8|Q3RCE8_XYLFA Putative uncharacterized protein OS=Xylel... 40 0.046
tr|Q3R1Z9|Q3R1Z9_XYLFA Putative uncharacterized protein OS=Xylel... 40 0.046
tr|Q9PGC0|Q9PGC0_XYLFA Putative uncharacterized protein OS=Xylel... 39 0.13
tr|Q5H2N3|Q5H2N3_XANOR Putative uncharacterized protein OS=Xanth... 39 0.13
tr|Q2P5K0|Q2P5K0_XANOM Putative uncharacterized protein XOO1422 ... 39 0.13
tr|B2SSG3|B2SSG3_XANOP Putative uncharacterized protein OS=Xanth... 39 0.13

>tr|B8LNB1|B8LNB1_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=4 SV=1
Length = 112

Score = 57.0 bits (136), Expect = 5e-07
Identities = 30/67 (44%), Positives = 35/67 (52%)
Frame = +1

Query: 154 YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 333
YP V CGRGDKRTAKGKRF S+GN+RPR K E +EI
Sbjct: 45 YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 334 IIDDNLF 354
ID++ F
Sbjct: 105 EIDESFF 111


>tr|A9NT69|A9NT69_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=4 SV=1
Length = 112

Score = 57.0 bits (136), Expect = 5e-07
Identities = 30/67 (44%), Positives = 35/67 (52%)
Frame = +1

Query: 154 YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 333
YP V CGRGDKRTAKGKRF S+GN+RPR K E +EI
Sbjct: 45 YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 334 IIDDNLF 354
ID++ F
Sbjct: 105 EIDESFF 111


>tr|A9NME7|A9NME7_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=4 SV=1
Length = 112

Score = 57.0 bits (136), Expect = 5e-07
Identities = 30/67 (44%), Positives = 35/67 (52%)
Frame = +1

Query: 154 YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEI 333
YP V CGRGDKRTAKGKRF S+GN+RPR K E +EI
Sbjct: 45 YPTVYCGRGDKRTAKGKRFKHSFGNARPRDNKKGRGLPITPLPPGAPKKDLFDDGEKIEI 104

Query: 334 IIDDNLF 354
ID++ F
Sbjct: 105 EIDESFF 111


>tr|B6UIF3|B6UIF3_MAIZE 30S ribosomal protein S31 OS=Zea mays PE=4
SV=1
Length = 88

Score = 55.5 bits (132), Expect = 1e-06
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = +1

Query: 136 LQASSAYPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
L A+S VTCGRGDK+T +GKRF GSYGN+RP++ K
Sbjct: 27 LAAASTAEAVTCGRGDKKTKRGKRFKGSYGNARPKREKK 65


>tr|A5C1F7|A5C1F7_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_030951 PE=4 SV=1
Length = 117

Score = 55.5 bits (132), Expect = 1e-06
Identities = 34/90 (37%), Positives = 43/90 (47%), Gaps = 3/90 (3%)
Frame = +1

Query: 94 SSSLMPPFSCNLLPLQASSA---YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXXX 264
S +L F + L S+ P V CGRGDK+TAKGKRF S+GN+RPR K
Sbjct: 27 SETLAVSFDTRFVSLSISATSPPIPSVYCGRGDKKTAKGKRFNHSFGNARPRNKKKGRGP 86

Query: 265 XXXXXXXXXXXXXXXXXXEFVEIIIDDNLF 354
E V+I ID++LF
Sbjct: 87 PRAPIPPSPPKKDRFEDDEVVKIEIDESLF 116


>tr|A7PP27|A7PP27_VITVI Chromosome chr8 scaffold_23, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00021486001
PE=4 SV=1
Length = 118

Score = 55.1 bits (131), Expect = 2e-06
Identities = 34/91 (37%), Positives = 43/91 (47%), Gaps = 4/91 (4%)
Frame = +1

Query: 94 SSSLMPPFSCNLLPLQASSA----YPLVTCGRGDKRTAKGKRFLGSYGNSRPRKPNKXXX 261
S +L F + L S+ P V CGRGDK+TAKGKRF S+GN+RPR K
Sbjct: 27 SETLAVSFDTRFVSLSISATSPPPIPSVYCGRGDKKTAKGKRFNHSFGNARPRNKKKGRG 86

Query: 262 XXXXXXXXXXXXXXXXXXXEFVEIIIDDNLF 354
E V+I ID++LF
Sbjct: 87 PPRAPIPPSPPKKDRFEDDEVVKIEIDESLF 117


>tr|A9TLI9|A9TLI9_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_196235 PE=4 SV=1
Length = 109

Score = 54.7 bits (130), Expect = 2e-06
Identities = 35/102 (34%), Positives = 51/102 (50%), Gaps = 8/102 (7%)
Frame = +1

Query: 82 TICASSSL----MPPFSCNLLPLQASSA----YPLVTCGRGDKRTAKGKRFLGSYGNSRP 237
++C SSS +P S + +SS+ P + CGRGDKRTAKGKR GS+GN RP
Sbjct: 10 SLCCSSSTFFKALPMPSAAAVSSSSSSSSSVSLPAIVCGRGDKRTAKGKRARGSFGNCRP 69

Query: 238 RKPNKXXXXXXXXXXXXXXXXXXXXXXEFVEIIIDDNLFKKK 363
+ +K EF+ + ID++LF ++
Sbjct: 70 KDSSK--GTGLAITPLPPRTKKEVEDTEFINVEIDESLFNRQ 109


>tr|Q0J066|Q0J066_ORYSJ Os09g0528100 protein OS=Oryza sativa subsp.
japonica GN=Os09g0528100 PE=4 SV=1
Length = 82

Score = 52.4 bits (124), Expect = 1e-05
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +1

Query: 163 VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
VTCGRGDK+T +GKRF GSYGN+RP++ K
Sbjct: 30 VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>tr|A2Z3B9|A2Z3B9_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_32120 PE=4 SV=1
Length = 82

Score = 52.4 bits (124), Expect = 1e-05
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +1

Query: 163 VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
VTCGRGDK+T +GKRF GSYGN+RP++ K
Sbjct: 30 VTCGRGDKKTKRGKRFKGSYGNARPKREKK 59


>tr|A7P7Y7|A7P7Y7_VITVI Chromosome chr3 scaffold_8, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00035968001
PE=4 SV=1
Length = 88

Score = 52.0 bits (123), Expect = 2e-05
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = +1

Query: 163 VTCGRGDKRTAKGKRFLGSYGNSRPRKPNK 252
+ CGRGDK+T KGKRF GSYGN+RP+K K
Sbjct: 36 ILCGRGDKKTKKGKRFKGSYGNARPKKEKK 65