| DK952695 |
| Clone id |
TST38A01NGRL0014_M19 |
| Library |
TST38 |
| Length |
582 |
| Definition |
Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_M19. 5' end sequence. |
| Accession |
DK952695 |
| Tissue type |
prothallia |
| Developmental stage |
gametophyte |
| Contig ID |
CL319Contig1 |
| Sequence |
CACCAAGCCCATGCCTGGCTCCCGCTGGATTAAGCTTCTTGTCGATGCCGGGTGTCGAGT
TGAGATTTGCACGGAAAAGAAGACTATTCTCAGTGTCGATGATATCAAACGTCTGATTGG
AAGGAAGTGTGATGGCGTGATTGGCCAGCTCACTGAAGACTGGGGAGATGGCCTCTTTTC
TTCCCTCAAAGCAGCCGGAGGCCATGCTTACAGCAACATGGCAGTTGGATACAACAATGT
TAACGTTGAGGCGGCCACACGTAACGGTGTAGCTGTAGGCAATACACCAGGGGTTCTTAC
CGAAACAACTGCCGAGCTTGCTGCGGCATTAACCTTAGCAGCTGCTCGACGGGTTGTTGA
AGCTGATTACTTCATGCGGGCAGGAAAATATGATGGCTGGCTTCCAACTTTGTTCATCGG
AAATTTGCTTAAAGGTCAGACAGTATGCATTATTGGAGCGGGAAGGATTGGTTCTGCCTA
CGCAAGGATGATGGTTGAAGGCTTTAAAATGAACGTTGTTTACTACGATCTCTATCACGC
AAGCAGACTTGAGAAGTTCGTAACAGCCTATGGCAAGTTTCT |
| ■■Homology search results ■■ |
- |
| Swiss-Prot (release 56.9) |
Link to BlastX Result : Swiss-Prot |
| sp_hit_id |
P13443 |
| Definition |
sp|P13443|DHGY_CUCSA Glycerate dehydrogenase OS=Cucumis sativus |
| Align length |
193 |
| Score (bit) |
275.0 |
| E-value |
1.0e-73 |
| Report |
 BLASTX 2.2.19 [Nov-02-2008]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DK952695|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0014_M19, 5'
(582 letters)
Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P13443|DHGY_CUCSA Glycerate dehydrogenase OS=Cucumis sativus ... 275 1e-73
sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase OS=Pyrococcus furiosus... 76 1e-13
sp|Q9C4M5|GYAR_THELI Glyoxylate reductase OS=Thermococcus litora... 75 2e-13
sp|B1L765|GYAR_KORCO Glyoxylate reductase OS=Korarchaeum cryptof... 75 3e-13
sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase OS=Pyrococcus kodakara... 74 7e-13
sp|O58320|GYAR_PYRHO Glyoxylate reductase OS=Pyrococcus horikosh... 73 9e-13
sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase OS=Pyrococcus abyssi G... 72 2e-12
sp|Q9YAW4|GYAR_AERPE Glyoxylate reductase OS=Aeropyrum pernix GN... 69 1e-11
sp|Q49ZM5|Y606_STAS1 Putative 2-hydroxyacid dehydrogenase SSP060... 62 2e-09
sp|Q2FVW4|Y2577_STAA8 Putative 2-hydroxyacid dehydrogenase SAOUH... 60 6e-09
sp|Q6GEC9|Y2389_STAAR Putative 2-hydroxyacid dehydrogenase SAR23... 60 6e-09
sp|Q99RW8|Y2305_STAAM Putative 2-hydroxyacid dehydrogenase SAV23... 60 6e-09
sp|Q5HDQ4|Y2296_STAAC Putative 2-hydroxyacid dehydrogenase SACOL... 60 6e-09
sp|Q2FEI9|Y2254_STAA3 Putative 2-hydroxyacid dehydrogenase SAUSA... 60 6e-09
sp|Q8NV80|Y2224_STAAW Putative 2-hydroxyacid dehydrogenase MW222... 60 6e-09
sp|Q6G716|Y2196_STAAS Putative 2-hydroxyacid dehydrogenase SAS21... 60 6e-09
sp|Q2YYT9|Y2178_STAAB Putative 2-hydroxyacid dehydrogenase SAB21... 60 6e-09
sp|Q7A417|Y2098_STAAN Putative 2-hydroxyacid dehydrogenase SA209... 60 6e-09
sp|Q5HLU4|Y1888_STAEQ Putative 2-hydroxyacid dehydrogenase SERP1... 60 6e-09
sp|Q8CNB8|Y1879_STAES Putative 2-hydroxyacid dehydrogenase SE_18... 60 6e-09
sp|A1RYE4|GYAR_THEPD Glyoxylate reductase OS=Thermofilum pendens... 59 1e-08
sp|Q4L8G4|Y752_STAHJ Putative 2-hydroxyacid dehydrogenase SH0752... 59 2e-08
sp|O32264|TKRA_BACSU Probable 2-ketogluconate reductase OS=Bacil... 57 5e-08
sp|Q59642|LDHD_PEDAC D-lactate dehydrogenase OS=Pediococcus acid... 57 9e-08
sp|P36234|DHGY_HYPME Glycerate dehydrogenase OS=Hyphomicrobium m... 55 3e-07
sp|O08651|SERA_RAT D-3-phosphoglycerate dehydrogenase OS=Rattus ... 54 6e-07
sp|Q61753|SERA_MOUSE D-3-phosphoglycerate dehydrogenase OS=Mus m... 54 7e-07
sp|Q60HD7|SERA_MACFA D-3-phosphoglycerate dehydrogenase OS=Macac... 54 7e-07
sp|Q8E0N5|LDHD_STRA5 D-lactate dehydrogenase OS=Streptococcus ag... 54 7e-07
sp|A5A6P1|SERA_PANTR D-3-phosphoglycerate dehydrogenase OS=Pan t... 53 1e-06
>sp|P13443|DHGY_CUCSA Glycerate dehydrogenase OS=Cucumis sativus
GN=HPR-A PE=2 SV=1
Length = 382
Score = 275 bits (703), Expect = 1e-73
Identities = 134/193 (69%), Positives = 152/193 (78%)
Frame = +2
Query: 2 TKPMPGSRWIKLLVDAGCRVEICTEKKTILSVDDIKRLIGRKCDGVIGQLTEDWGDGLFS 181
TKPMPG+RWI LL++ CRVEICTEKKTILSV+DI LIG KCDGVIGQLTEDWG+ LFS
Sbjct: 21 TKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDILALIGDKCDGVIGQLTEDWGEVLFS 80
Query: 182 SLKAAGGHAYSNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXX 361
+L AGG A+SNMAVGYNNV+V AA + GVAVGNTPG
Sbjct: 81 ALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVE 140
Query: 362 XDYFMRAGKYDGWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHA 541
D FMRAG+YDGWLP LF+GNLLKGQTV +IGAGRIGSAYARMMVEGFKMN++Y+DLY +
Sbjct: 141 ADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQS 200
Query: 542 SRLEKFVTAYGKF 580
+RLEKFVTAYG+F
Sbjct: 201 TRLEKFVTAYGEF 213
>sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase OS=Pyrococcus furiosus
GN=gyaR PE=3 SV=1
Length = 336
Score = 75.9 bits (185), Expect = 1e-13
Identities = 53/170 (31%), Positives = 83/170 (48%), Gaps = 4/170 (2%)
Frame = +2
Query: 59 VEICTEKKTILSVDDIKRLIGRKCDGVIGQLTEDWGDGLFSSLKAAGGHAYSNMAVGYNN 238
VE+ E++ I ++++ + D ++ L+E +F + A +N AVGY+N
Sbjct: 25 VEVWEEEREIPREKLLEKV--KDVDALVTMLSERIDQEVFEN--APRLRIVANYAVGYDN 80
Query: 239 VNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKYD----GWLP 406
++VE ATR G+ V NTP D F+R+G++ W P
Sbjct: 81 IDVEEATRRGIYVTNTPDVLTNATADHAFALLLATARHVVKGDKFVRSGEWKRKGIAWHP 140
Query: 407 TLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEK 556
F+G L G+T+ I+G GRIG A AR +GF M ++YY S+ EK
Sbjct: 141 KWFLGYELYGKTIGIVGFGRIGQAIAR-RAKGFNMRILYYSRTRKSQAEK 189
>sp|Q9C4M5|GYAR_THELI Glyoxylate reductase OS=Thermococcus litoralis
GN=gyaR PE=1 SV=1
Length = 331
Score = 75.1 bits (183), Expect = 2e-13
Identities = 42/120 (35%), Positives = 63/120 (52%), Gaps = 4/120 (3%)
Frame = +2
Query: 221 AVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKYD-- 394
AVGY+N+++E AT+ G+ V NTPG D F+R+G++
Sbjct: 75 AVGYDNIDIEEATKRGIYVTNTPGVLTDATADLAFALLLAVARRIVEADAFVRSGEWKKS 134
Query: 395 --GWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEKFVTA 568
GW P +F+G LKG+T+ I+G GRIG A A+ +GF M ++YY E+ + A
Sbjct: 135 EVGWHPLMFLGYGLKGKTLGIVGFGRIGQALAK-RAKGFGMKIIYYSRTRKPEAEEEIGA 193
>sp|B1L765|GYAR_KORCO Glyoxylate reductase OS=Korarchaeum
cryptofilum (strain OPF8) GN=gyaR PE=3 SV=1
Length = 332
Score = 74.7 bits (182), Expect = 3e-13
Identities = 46/146 (31%), Positives = 72/146 (49%), Gaps = 1/146 (0%)
Frame = +2
Query: 122 RKCDGVIGQLTEDWGDGLFSSLKAAGGHAYSNMAVGYNNVNVEAATRNGVAVGNTPGXXX 301
+ CD ++ LT+ +F + A + AVGY+N++V+ AT+ G+ V NTPG
Sbjct: 44 KDCDALVSLLTDPIDAEVFEA--APKLRIVAQYAVGYDNIDVKEATKRGIYVTNTPGVLT 101
Query: 302 XXXXXXXXXXXXXXXXXXXXXDYFMRAGKYD-GWLPTLFIGNLLKGQTVCIIGAGRIGSA 478
D ++R GK+ W P + +G + G+T+ I+G GRIG+A
Sbjct: 102 ETTADFAFALLMAAARRVVEADRYVREGKWKVAWHPMMMLGYDVYGRTLGIVGMGRIGAA 161
Query: 479 YARMMVEGFKMNVVYYDLYHASRLEK 556
AR +GF M ++YYD EK
Sbjct: 162 VAR-RAKGFGMRILYYDSIRREDFEK 186
>sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase OS=Pyrococcus
kodakaraensis GN=gyaR PE=3 SV=1
Length = 333
Score = 73.6 bits (179), Expect = 7e-13
Identities = 53/170 (31%), Positives = 80/170 (47%), Gaps = 4/170 (2%)
Frame = +2
Query: 59 VEICTEKKTILSVDDIKRLIGRKCDGVIGQLTEDWGDGLFSSLKAAGGHAYSNMAVGYNN 238
VE+ E++ I +K++ R D ++ L+E +F + A +N AVGY+N
Sbjct: 25 VEVWPEEREIPREVLLKKV--RDVDALVTMLSERIDSEVFDA--APRLRIVANYAVGYDN 80
Query: 239 VNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKYD----GWLP 406
++VE ATR G+ V NTP D+F R+G++ W P
Sbjct: 81 IDVEEATRRGIYVTNTPDVLTDATADFAWTLLLATARRLIEADHFTRSGEWKRRGIAWHP 140
Query: 407 TLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEK 556
F+G + G+T+ I+G GRIG A AR GF M ++YY EK
Sbjct: 141 RWFLGYDVYGKTIGIVGFGRIGQAVAR-RARGFGMRILYYSRSRKPEAEK 189
>sp|O58320|GYAR_PYRHO Glyoxylate reductase OS=Pyrococcus horikoshii
GN=gyaR PE=1 SV=2
Length = 334
Score = 73.2 bits (178), Expect = 9e-13
Identities = 54/184 (29%), Positives = 90/184 (48%), Gaps = 4/184 (2%)
Frame = +2
Query: 29 IKLLVDAGCRVEICTEKKTILSVDDIKRLIGRKCDGVIGQLTEDWGDGLFSSLKAAGGHA 208
IK+L D VE+ ++K I +K++ ++ D ++ L+E +F + A
Sbjct: 16 IKMLEDE-FEVEVWGDEKEIPREILLKKV--KEVDALVTMLSERIDKEVFEN--APKLRI 70
Query: 209 YSNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGK 388
+N AVGY+N+++E AT+ G+ V NTP D F+R+G+
Sbjct: 71 VANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKGDRFVRSGE 130
Query: 389 YD----GWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEK 556
+ W P F+G + G+T+ IIG GRIG A A+ +GF M ++YY +E+
Sbjct: 131 WKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAK-RAKGFNMRILYYSRTRKEEVER 189
Query: 557 FVTA 568
+ A
Sbjct: 190 ELNA 193
>sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase OS=Pyrococcus abyssi
GN=gyaR PE=3 SV=1
Length = 335
Score = 72.0 bits (175), Expect = 2e-12
Identities = 43/123 (34%), Positives = 61/123 (49%), Gaps = 4/123 (3%)
Frame = +2
Query: 212 SNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKY 391
+N AVGY+N++VE AT+ G+ V NTPG D F R+G++
Sbjct: 73 ANYAVGYDNIDVEEATKRGIYVTNTPGVLTDATADLAFALLLATARHLVKGDKFTRSGEW 132
Query: 392 D----GWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEKF 559
W P F+G + G+T+ IIG GRIG A A+ GF M ++YY +EK
Sbjct: 133 KKRGVAWHPKWFLGYDVYGKTIGIIGFGRIGQAIAK-RARGFDMRILYYSRTRKPEVEKE 191
Query: 560 VTA 568
+ A
Sbjct: 192 LNA 194
>sp|Q9YAW4|GYAR_AERPE Glyoxylate reductase OS=Aeropyrum pernix
GN=gyaR PE=3 SV=2
Length = 335
Score = 69.3 bits (168), Expect = 1e-11
Identities = 40/119 (33%), Positives = 62/119 (52%), Gaps = 4/119 (3%)
Frame = +2
Query: 212 SNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKY 391
+ MAVG++N++VE ATR G+ V NTPG D+F+R G++
Sbjct: 73 AQMAVGFDNIDVECATRLGIYVTNTPGVLTEATAEFTWALILAAARRVVEADHFVRWGEW 132
Query: 392 ----DGWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVYYDLYHASRLEK 556
GW P + +G L+G+T+ I+G GRIGS A + + F M ++Y+ +EK
Sbjct: 133 WRLRTGWHPMMMLGVELRGKTLGILGMGRIGSRVAE-IGKAFGMRIIYHSRSRKREIEK 190
>sp|Q49ZM5|Y606_STAS1 Putative 2-hydroxyacid dehydrogenase SSP0606
OS=Staphylococcus saprophyticus subsp. saprophyticus
(strain ATCC 15305 / DSM 20229) GN=SSP0606 PE=3 SV=1
Length = 318
Score = 62.0 bits (149), Expect = 2e-09
Identities = 46/165 (27%), Positives = 73/165 (44%)
Frame = +2
Query: 29 IKLLVDAGCRVEICTEKKTILSVDDIKRLIGRKCDGVIGQLTEDWGDGLFSSLKAAGGHA 208
+ LL D G V++ E I+ + +K+ + + D +I L+ + + A
Sbjct: 15 LNLLKDQGFEVDMY-EGTGIIDKETLKQGV-KDADALISLLSTSVDKEVIDA--ANNLKI 70
Query: 209 YSNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGK 388
+N G+NNV+++ A + + V NTP D R
Sbjct: 71 ITNYGAGFNNVDIDYARQQNIDVTNTPKASTNSTAELTFALVLAVARRIPEGDKLCRTTG 130
Query: 389 YDGWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVY 523
+DGW P F G + G+T+ IIG G IGSA AR + F MN++Y
Sbjct: 131 FDGWAPLFFRGREVSGKTIGIIGLGEIGSAVAR-RAKAFDMNILY 174
>sp|Q2FVW4|Y2577_STAA8 Putative 2-hydroxyacid dehydrogenase
SAOUHSC_02577 OS=Staphylococcus aureus (strain NCTC
8325) GN=SAOUHSC_02577 PE=3 SV=1
Length = 317
Score = 60.5 bits (145), Expect = 6e-09
Identities = 34/104 (32%), Positives = 46/104 (44%)
Frame = +2
Query: 212 SNMAVGYNNVNVEAATRNGVAVGNTPGXXXXXXXXXXXXXXXXXXXXXXXXDYFMRAGKY 391
+N G+NN+++E A + V NTP D R +
Sbjct: 71 ANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGF 130
Query: 392 DGWLPTLFIGNLLKGQTVCIIGAGRIGSAYARMMVEGFKMNVVY 523
DGW P F G + G+T+ IIG G IGSA AR F M+V+Y
Sbjct: 131 DGWAPLFFRGREVSGKTIGIIGLGEIGSAVAR-RARAFDMDVLY 173
|
| TrEMBL (release 39.9) |
Link to BlastX Result : TrEMBL |
| tr_hit_id |
A9U188 |
| Definition |
tr|A9U188|A9U188_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens |
| Align length |
193 |
| Score (bit) |
287.0 |
| E-value |
3.0e-76 |
| Report |
|