DK952718
Clone id TST38A01NGRL0014_N19
Library
Length 658
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_N19. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence
GCCCACAACATGAGGAAGGATGAAGCAGGGCATTAGAGAAAGTCCAGGATAACCCAAAAT
ACTAAGCTACTTCAGAAATCTTAGTGTTAACCTTACTTTTAGGTCAGCTCTTGCAAGAAA
CAGGCAGCCTTTGCAAATAAATTTAACCTTGCTAAAGTTGCTGCTAATCACTACCCAAAT
TTTGTGATTCTAAGTAAGAGTGCACTCCAACAGCTAGACAGAAATTTTCAAAGTAGTGTG
AGCAAGTATGATAGATGGATGCCCCGATGGATGGGCGGGCGGAATCCCGGTCTGCGTCCT
CTCCGGCTCTGCCCAGCAGGACAACACCAATGTTTGTGCATCAGCTAGAGCTGCTGGTAA
AGATGGAACTTTAGTGAAAGAGAGAGACTACATCAGCCTCGGGCTGACGGGCGCAGCACC
AGAACAAATGCTTACAAGTTCCATCCCTTCTGGTGTTGATAAAATGGAGTGTGATAATGT
GGTGCTAAGCCTTGGCTTAGGCCCCCCTGCAGATAAAGCAGCAGCTGCTTCCCAAGAGCA
GAACACCGGCATTGTTGACCAGCAAATTAGGCTCAAGGTCGCTATGACTAGCGCAAAACC
GCAGCACTTTTGGCATGAGAGCTTCCGCGTGAGCCTCAGCAATGATCAATATCATCAG
■■Homology search results ■■ -
sp_hit_id Q54Z69
Definition sp|Q54Z69|RL4_DICDI 60S ribosomal protein L4 OS=Dictyostelium discoideum
Align length 56
Score (bit) 32.3
E-value 2.3
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK952718|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0014_N19, 5'
(658 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q54Z69|RL4_DICDI 60S ribosomal protein L4 OS=Dictyostelium di... 32 2.3
sp|Q9D6Z1|NOL5A_MOUSE Nucleolar protein 5A OS=Mus musculus GN=No... 32 2.3
sp|Q95K50|NOL5A_MACFA Nucleolar protein 5A OS=Macaca fasciculari... 32 2.3
sp|O00567|NOL5A_HUMAN Nucleolar protein 5A OS=Homo sapiens GN=NO... 32 2.3
sp|Q3SZ63|NOL5A_BOVIN Nucleolar protein 5A OS=Bos taurus GN=NOL5... 32 2.3
sp|Q8PCJ8|DDLB_XANCP D-alanine--D-alanine ligase B OS=Xanthomona... 31 6.6
sp|P55200|HRX_MOUSE Histone-lysine N-methyltransferase HRX OS=Mu... 30 8.6

>sp|Q54Z69|RL4_DICDI 60S ribosomal protein L4 OS=Dictyostelium
discoideum GN=rpl4 PE=3 SV=1
Length = 369

Score = 32.3 bits (72), Expect = 2.3
Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 5/56 (8%)
Frame = +1

Query: 121 QAAFANKFNLAKVA-ANHYPNFVILSKSALQQLDRNF----QSSVSKYDRWMPRWM 273
+ A ++ NL K+A H F+I +KSA +QLD F +SS K +PR M
Sbjct: 227 EVANVSRLNLLKLAPGGHLGRFIIWTKSAFEQLDSTFGTFAKSSAQKKGYTLPRPM 282


>sp|Q9D6Z1|NOL5A_MOUSE Nucleolar protein 5A OS=Mus musculus GN=Nol5a
PE=1 SV=2
Length = 580

Score = 32.3 bits (72), Expect = 2.3
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1

Query: 103 VSSCKKQAAFANKFNLAKVA--ANHYPNFVILSKSALQQLDRNFQSSVSKYDRW 258
+S+CK Q + ++ AKV N N +I S S L QLD++ + + W
Sbjct: 139 LSACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREW 192


>sp|Q95K50|NOL5A_MACFA Nucleolar protein 5A OS=Macaca fascicularis
GN=NOL5A PE=2 SV=1
Length = 594

Score = 32.3 bits (72), Expect = 2.3
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1

Query: 103 VSSCKKQAAFANKFNLAKVA--ANHYPNFVILSKSALQQLDRNFQSSVSKYDRW 258
+S+CK Q + ++ AKV N N +I S S L QLD++ + + W
Sbjct: 139 LSACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREW 192


>sp|O00567|NOL5A_HUMAN Nucleolar protein 5A OS=Homo sapiens GN=NOL5A
PE=1 SV=4
Length = 594

Score = 32.3 bits (72), Expect = 2.3
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1

Query: 103 VSSCKKQAAFANKFNLAKVA--ANHYPNFVILSKSALQQLDRNFQSSVSKYDRW 258
+S+CK Q + ++ AKV N N +I S S L QLD++ + + W
Sbjct: 139 LSACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREW 192


>sp|Q3SZ63|NOL5A_BOVIN Nucleolar protein 5A OS=Bos taurus GN=NOL5A
PE=2 SV=1
Length = 596

Score = 32.3 bits (72), Expect = 2.3
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1

Query: 103 VSSCKKQAAFANKFNLAKVA--ANHYPNFVILSKSALQQLDRNFQSSVSKYDRW 258
+S+CK Q + ++ AKV N N +I S S L QLD++ + + W
Sbjct: 139 LSACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREW 192


>sp|Q8PCJ8|DDLB_XANCP D-alanine--D-alanine ligase B OS=Xanthomonas
campestris pv. campestris GN=ddlB PE=3 SV=1
Length = 318

Score = 30.8 bits (68), Expect = 6.6
Identities = 37/135 (27%), Positives = 58/135 (42%), Gaps = 3/135 (2%)
Frame = +2

Query: 200 VHSNS*TEIFKVV*ASMIDGCPDGWAGGIPVCVLSGSAQQDNT--NVCASARAAGKDGTL 373
V NS + + + A +D P GIP + AQ+ + NV G+DG +
Sbjct: 32 VSLNSGSNVLDALRARGVDAQP---VDGIPALAQALVAQRFDRVFNVLHGHNGGGEDGIV 88

Query: 374 VKERDYISLGLTGAAPEQMLTSSIPSGVDKMECDNVVLSLGLGPPA-DKAAAASQEQNTG 550
+ + TG+ +L S++ +DK+ V LSLGL P + AA + Q
Sbjct: 89 QGLMEAFGVPYTGS---NVLGSAL--SMDKIRTKQVWLSLGLSTPRYARLAAGASAQQIH 143

Query: 551 IVDQQIRLKVAMTSA 595
+QI L V + A
Sbjct: 144 AAAEQIGLPVIVKPA 158


>sp|P55200|HRX_MOUSE Histone-lysine N-methyltransferase HRX OS=Mus
musculus GN=Mll PE=2 SV=2
Length = 3966

Score = 30.4 bits (67), Expect = 8.6
Identities = 27/104 (25%), Positives = 41/104 (39%), Gaps = 1/104 (0%)
Frame = +2

Query: 242 ASMIDGCPDGWAGGIPVCVLSGSAQQDNTNVCASARAAGKDGTLVKERDYISLGLTGAAP 421
AS I P G+ G A++ + AGK GTL +RD G P
Sbjct: 3420 ASSICVLPSSQTAGMTAASPPGEAEEHYKLQRGNQLLAGKTGTLTSQRDRDPDSAPGTQP 3479

Query: 422 EQML-TSSIPSGVDKMECDNVVLSLGLGPPADKAAAASQEQNTG 550
T+ P+GV ++E + + S A ++ S Q +G
Sbjct: 3480 SNFTQTAEAPNGV-RLEQNKTLPSAKPASSASPGSSPSSGQQSG 3522


tr_hit_id A8J5T5
Definition tr|A8J5T5|A8J5T5_CHLRE COP-II coat subunit OS=Chlamydomonas reinhardtii
Align length 104
Score (bit) 38.9
E-value 0.28
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK952718|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0014_N19, 5'
(658 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A8J5T5|A8J5T5_CHLRE COP-II coat subunit OS=Chlamydomonas rein... 39 0.28
tr|A6SWV7|A6SWV7_JANMA Multidrug efflux system transmembrane pro... 37 1.4
tr|Q39TW1|Q39TW1_GEOMG 4Fe-4S ferredoxin, iron-sulfur binding pr... 35 3.1
tr|A1R9P9|A1R9P9_ARTAT Nitrite reductase [NAD(P)H], large subuni... 35 3.1
tr|B1J457|B1J457_PSEPW FAD-dependent pyridine nucleotide-disulph... 35 5.3
tr|Q1I2R6|Q1I2R6_PSEE4 Rubredoxin-NAD(+) reductase OS=Pseudomona... 34 7.0
tr|B5E7S9|B5E7S9_GEOBB 4Fe-4S ferredoxin iron-sulfur binding dom... 34 7.0
tr|Q0YNI7|Q0YNI7_9DELT 4Fe-4S ferredoxin, iron-sulfur binding OS... 34 7.0
tr|B3JSZ6|B3JSZ6_9DELT 4Fe-4S ferredoxin iron-sulfur binding dom... 34 9.1

>tr|A8J5T5|A8J5T5_CHLRE COP-II coat subunit OS=Chlamydomonas
reinhardtii GN=SEC31 PE=4 SV=1
Length = 1313

Score = 38.9 bits (89), Expect = 0.28
Identities = 35/104 (33%), Positives = 43/104 (41%), Gaps = 16/104 (15%)
Frame = +2

Query: 308 SAQQDNTNVCASARAAGKDGTLVKERDYISL---------GLTGAAPEQMLTSSIPSGVD 460
SA T A AAG D ++ D + G A+P Q+L P D
Sbjct: 508 SAAGGPTENGVDAAAAGMDQLNLQNADGVPRLEVPGASVGGAVAASPHQLLAQDDPDFFD 567

Query: 461 KMECDNVVLSLGLGPPADKAAAAS-------QEQNTGIVDQQIR 571
K+E VVL GL P AD AAAA+ Q N G +D R
Sbjct: 568 KLEDTEVVLG-GLAPDADVAAAAAGPDLGGVQPLNAGAMDAMAR 610


>tr|A6SWV7|A6SWV7_JANMA Multidrug efflux system transmembrane
protein OS=Janthinobacterium sp. (strain Marseille)
(Minibacterium massiliensis) GN=mma_1064 PE=4 SV=1
Length = 570

Score = 36.6 bits (83), Expect = 1.4
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 2/82 (2%)
Frame = +2

Query: 302 SGSAQQDNTNV--CASARAAGKDGTLVKERDYISLGLTGAAPEQMLTSSIPSGVDKMECD 475
SG A+Q T + + GKDG + E+ + LG+T Q+L SG++ E D
Sbjct: 483 SGFAEQSTTRTPRTGTVKVIGKDGNI--EQRKVELGVTNRVQMQVL-----SGLE--EGD 533

Query: 476 NVVLSLGLGPPADKAAAASQEQ 541
VVL + L P + AAA+ +Q
Sbjct: 534 TVVLGMKLPPSTKRPAAATNQQ 555


>tr|Q39TW1|Q39TW1_GEOMG 4Fe-4S ferredoxin, iron-sulfur binding
protein OS=Geobacter metallireducens (strain GS-15 /
ATCC 53774 / DSM 7210) GN=Gmet_2084 PE=4 SV=1
Length = 1014

Score = 35.4 bits (80), Expect = 3.1
Identities = 20/53 (37%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 RDYISLGLTGAAPEQMLT--SSIPSGVDKMECDNVVLSLGLGPPADKAAAASQ 535
R Y+++G + + + S++ GV + E D VVLS+GL PP D A AS+
Sbjct: 325 RSYVTIGREIESTKNVTIRYSTVDDGVKEEEFDMVVLSVGLNPPKDVEALASK 377


>tr|A1R9P9|A1R9P9_ARTAT Nitrite reductase [NAD(P)H], large subunit
OS=Arthrobacter aurescens (strain TC1) GN=AAur_3265 PE=4
SV=1
Length = 826

Score = 35.4 bits (80), Expect = 3.1
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 9/109 (8%)
Frame = +2

Query: 275 AGGIPVCVLSGSAQQDNTNVCASARAAGKDGTLVKERDYISLGL---TGAAPEQMLTSSI 445
A G+ V V+ N + G DG + R +LG+ TG+ +L S
Sbjct: 178 ANGLDVAVVHSGGHLMNAQL-------GPDGGAILRRSVEALGIEVHTGSRTTAILGSDQ 230

Query: 446 PSGV-----DKMECDNVVLSLGLGPPADKAAAASQEQNTGI-VDQQIRL 574
SGV +++C+ VV++ G+ P D A + GI VD Q+R+
Sbjct: 231 LSGVRLRDDTEIDCNMVVVTAGIRPNVDVAVVSGLAVERGIVVDDQLRV 279


>tr|B1J457|B1J457_PSEPW FAD-dependent pyridine nucleotide-disulphide
oxidoreductase OS=Pseudomonas putida (strain W619)
GN=PputW619_0159 PE=4 SV=1
Length = 382

Score = 34.7 bits (78), Expect = 5.3
Identities = 18/35 (51%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
Frame = +2

Query: 470 CDNVVLSLGLGPPADKAAAASQEQNTG-IVDQQIR 571
CD VV ++GL P D AAAA + N G +VD+Q+R
Sbjct: 230 CDLVVSAIGLRPRTDLAAAAGLQTNRGVVVDRQLR 264


>tr|Q1I2R6|Q1I2R6_PSEE4 Rubredoxin-NAD(+) reductase OS=Pseudomonas
entomophila (strain L48) GN=rubB PE=4 SV=1
Length = 382

Score = 34.3 bits (77), Expect = 7.0
Identities = 18/35 (51%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
Frame = +2

Query: 470 CDNVVLSLGLGPPADKAAAASQEQNTG-IVDQQIR 571
CD VV ++GL P D AAAA + N G +VD+Q+R
Sbjct: 230 CDLVVSAIGLRPRTDLAAAAGLQVNRGVVVDRQLR 264


>tr|B5E7S9|B5E7S9_GEOBB 4Fe-4S ferredoxin iron-sulfur binding domain
protein OS=Geobacter bemidjiensis (strain Bem / ATCC
BAA-1014 / DSM 16622) GN=Gbem_1447 PE=4 SV=1
Length = 1012

Score = 34.3 bits (77), Expect = 7.0
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 RDYISLG--LTGAAPEQMLTSSIPSGVDKMECDNVVLSLGLGPPADKAAAASQ 535
R Y+++G + + + S++ GV + E D VVLS+GL PP D A A++
Sbjct: 325 RSYVTVGREIESSKNVTIRYSTVDQGVKEEEFDMVVLSVGLNPPKDVEALATK 377


>tr|Q0YNI7|Q0YNI7_9DELT 4Fe-4S ferredoxin, iron-sulfur binding
OS=Geobacter sp. FRC-32 GN=GeobDRAFT_3317 PE=4 SV=1
Length = 1018

Score = 34.3 bits (77), Expect = 7.0
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = +2

Query: 383 RDYISLGLTGAAPEQMLT--SSIPSGVDKMECDNVVLSLGLGPPADKAAAAS 532
R Y+S+G + S+ GV + E D VVLS+GL PPAD A+
Sbjct: 324 RSYVSIGRENPETRNVSIRYSTFDQGVKEEEFDMVVLSVGLNPPADVQTMAT 375


>tr|B3JSZ6|B3JSZ6_9DELT 4Fe-4S ferredoxin iron-sulfur binding domain
protein OS=Geobacter sp. M21 GN=GM21DRAFT_0691 PE=4 SV=1
Length = 1012

Score = 33.9 bits (76), Expect = 9.1
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 RDYISLG--LTGAAPEQMLTSSIPSGVDKMECDNVVLSLGLGPPADKAAAASQ 535
R Y+++G + + + S++ GV + E D VVLS+GL PP D A A +
Sbjct: 325 RSYVTVGREIESSKNVTIRYSTVDQGVKEEEFDMVVLSVGLNPPKDVEALAEK 377