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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK952946
Clone id TST38A01NGRL0015_H13
Library
Length 591
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0015_H13. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GAGGAGAATGAGATCTCTAACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTTGTAGGCG
GTGGCCCGGTTAAGGGGGAGAACCCCCGGAGCCGTAGCGAAAGCGAGTCTTGCTGGGGCA
ATTGTCACCGCTTATAAACCCGAACCTGGGTGATCTATCCATGACCAGGATGAAGCTTGG
GTGAAACTAAGTGGAGGTCCGAACCGACTGATGTTGAAAAATCAGCGGATGAGTTGTGGT
TAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGC
GCAGCAGTTGACTAGACCATCTAGGGGTAAAGCACCGTTCCGGTGCGGGCTGCGAGAGCG
GTACCAAATCGAGGCAAACTCTGAATACTAGATGTAACGTTCAAGTAAAAGGAACGAAGG
AAAAGGGTCAACCAGTGAGACGATGAGGGATAAGCTTCACCGTCGAGAGGGGAACAGCCC
AGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGGTAAAGGAGGTAGGAGTGCAAAG
ACAGCCAGGAGGTTTGCCTAGAAGCAGCCACCCTTGAAAGAGTGGCGTAAT
■■Homology search results ■■ -
sp_hit_id Q99819
Definition sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo sapiens
Align length 26
Score (bit) 30.8
E-value 5.3
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK952946|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0015_H13, 5'
         (591 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo s...    31   5.3  
sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas s...    31   5.3  
sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas s...    31   5.3  
sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus ...    30   6.9  
sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas s...    30   7.0  
sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas s...    30   7.0  
sp|Q5R108|RECO_IDILO DNA repair protein recO OS=Idiomarina loihi...    30   9.0  
sp|Q8UVR5|BAZ1A_XENLA Bromodomain adjacent to zinc finger domain...    30   9.0  

>sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo
           sapiens GN=ARHGDIG PE=2 SV=2
          Length = 225

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 13/26 (50%), Positives = 20/26 (76%)
 Frame = +2

Query: 101 KRVLLGQLSPLINPNLGDLSMTRMKL 178
           KRVLLG L P ++P+L ++ +TR+ L
Sbjct: 73  KRVLLGPLPPAVDPSLPNVQVTRLTL 98


>sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. tomato GN=gshA PE=3 SV=1
          Length = 529

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +1

Query: 106 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 234
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 263 SLRKAVATPYKPYVDIGTHDQNGEWVQLN--TNVLQIENEYYS 303


>sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. phaseolicola (strain 1448A / Race 6)
           GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +1

Query: 106 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 234
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVDVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus
           GN=Tmem201 PE=2 SV=1
          Length = 664

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -2

Query: 131 AVTIAPARLAFATAPGVLPLNRATAYKSPAHSS 33
           +VT +PA L   T PG LPL +   ++SP   S
Sbjct: 409 SVTSSPASLFIPTPPGFLPLTKQQLFRSPRRVS 441


>sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. syringae (strain B728a) GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.4 bits (67), Expect = 7.0
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +1

Query: 106 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 234
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. syringae GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.4 bits (67), Expect = 7.0
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +1

Query: 106 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 234
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|Q5R108|RECO_IDILO DNA repair protein recO OS=Idiomarina
           loihiensis GN=recO PE=3 SV=1
          Length = 234

 Score = 30.0 bits (66), Expect = 9.0
 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 3/79 (3%)
 Frame = -3

Query: 367 LVPLSQPAPERCFTPRWSSQLLRLNAFRGEPASSGFEWHFTPNHNSSADFSTSVGSDLHL 188
           LVP    APE  F    S+     N+ R EPA   FEW    +  ++ D+     +   L
Sbjct: 99  LVPTEAEAPE-LFESYLSALQNMTNSERVEPALRQFEWQLLQHLGAAFDWHYDADTGNVL 157

Query: 187 VSPK---LHPGHG*ITQVR 140
              K     P HG +TQ R
Sbjct: 158 SDSKWCYFIPDHGFVTQAR 176


>sp|Q8UVR5|BAZ1A_XENLA Bromodomain adjacent to zinc finger domain
           protein 1A (Fragment) OS=Xenopus laevis GN=baz1a PE=2
           SV=1
          Length = 627

 Score = 30.0 bits (66), Expect = 9.0
 Identities = 22/96 (22%), Positives = 39/96 (40%), Gaps = 1/96 (1%)
 Frame = +2

Query: 239 VRGEMPLEPRASWFSPKCVEAQQLTR-PSRGKAPFRCGLRERYQIEANSEY*M*RSSKRN 415
           VR ++   P   WF P+C   Q+  R PS          R RY ++++ E       K  
Sbjct: 252 VRPKLKYVPEGDWFCPECHPKQRSHRLPS----------RHRYSMDSDEEEEEELDQKEE 301

Query: 416 EGKGSTSETMRDKLHRREGNSPDHQLRPLNDRSVVK 523
           E +    E + +  + +E    + +  P   R+ V+
Sbjct: 302 EEEEEEQEELSESENEQEDEMSEEESPPKRGRAKVQ 337
tr_hit_id Q5M9Z9
Definition tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b OS=Nicotiana tabacum
Align length 37
Score (bit) 77.4
E-value 2.0e-34
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK952946|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0015_H13, 5'
         (591 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b ...    77   2e-34
tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa s...   136   8e-31
tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medic...   129   2e-28
tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whol...    77   1e-19
tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1            87   5e-16
tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein OS=Rumin...    79   1e-13
tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein OS=Rumin...    79   1e-13
tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein OS=Rumin...    77   9e-13
tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein OS=Rumin...    72   3e-12
tr|B1BC32|B1BC32_CLOBO Putative uncharacterized protein OS=Clost...    45   4e-10
tr|Q85WS9|Q85WS9_PINKO ORF40u OS=Pinus koraiensis PE=4 SV=2            65   2e-09
tr|A7PNF4|A7PNF4_VITVI Chromosome chr1 scaffold_22, whole genome...    65   3e-09
tr|A5KR91|A5KR91_9FIRM Putative uncharacterized protein OS=Rumin...    62   3e-09
tr|A7QY24|A7QY24_VITVI Chromosome undetermined scaffold_237, who...    52   9e-09
tr|B0A7G6|B0A7G6_9CLOT Putative uncharacterized protein OS=Clost...    62   2e-08
tr|A5KRH1|A5KRH1_9FIRM Putative uncharacterized protein OS=Rumin...    62   2e-08
tr|B2PX02|B2PX02_PROST Putative uncharacterized protein OS=Provi...    62   3e-08
tr|A7B5W8|A7B5W8_RUMGN Putative uncharacterized protein OS=Rumin...    57   6e-08
tr|Q0JIM3|Q0JIM3_ORYSJ Photosystem I P700 chlorophyll A apoprote...    60   9e-08
tr|B0NEY0|B0NEY0_EUBSP Putative uncharacterized protein OS=Clost...    54   4e-07
tr|A2PD01|A2PD01_VIBCH Putative uncharacterized protein OS=Vibri...    57   6e-07
tr|Q9RXQ4|Q9RXQ4_DEIRA Putative uncharacterized protein OS=Deino...    56   2e-06
tr|Q9RS75|Q9RS75_DEIRA Putative uncharacterized protein OS=Deino...    56   2e-06
tr|A8URE2|A8URE2_9AQUI Putative uncharacterized protein OS=Hydro...    55   4e-06
tr|Q3BKI1|Q3BKI1_9PROT Putative uncharacterized protein OS=Magne...    53   1e-05
tr|A9PE47|A9PE47_POPTR Putative uncharacterized protein OS=Popul...    53   1e-05
tr|B4VCZ2|B4VCZ2_9ACTO Putative uncharacterized protein OS=Strep...    53   1e-05
tr|A4ECR2|A4ECR2_9ACTN Putative uncharacterized protein OS=Colli...    53   1e-05
tr|B1BE08|B1BE08_CLOBO Putative uncharacterized protein OS=Clost...    51   3e-05
tr|B7R8W0|B7R8W0_9THEO Putative uncharacterized protein OS=Carbo...    47   6e-04

>tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b
           OS=Nicotiana tabacum GN=orf106b PE=4 SV=1
          Length = 106

 Score = 77.4 bits (189), Expect(3) = 2e-34
 Identities = 35/37 (94%), Positives = 36/37 (97%)
 Frame = -1

Query: 312 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSV 202
           VNCCASTHFGENQLALG+SGISPLTTTHPLI QHQSV
Sbjct: 48  VNCCASTHFGENQLALGASGISPLTTTHPLILQHQSV 84



 Score = 60.8 bits (146), Expect(3) = 2e-34
 Identities = 31/47 (65%), Positives = 35/47 (74%)
 Frame = -3

Query: 460 VKLIPHRLTG*PFSFVPFT*TLHLVFRVCLDLVPLSQPAPERCFTPR 320
           +KLIPHRLT  P    P     +LVFRVCLDLVPLS+PAP+ CFTPR
Sbjct: 1   MKLIPHRLTSRPCP--PLILRSYLVFRVCLDLVPLSRPAPKPCFTPR 45



 Score = 52.0 bits (123), Expect(3) = 2e-34
 Identities = 21/21 (100%), Positives = 21/21 (100%)
 Frame = -2

Query: 200 GPPLSFTQASSWSWIDHPGSG 138
           GPPLSFTQASSWSWIDHPGSG
Sbjct: 85  GPPLSFTQASSWSWIDHPGSG 105


>tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa
           subsp. indica PE=4 SV=1
          Length = 109

 Score =  136 bits (343), Expect = 8e-31
 Identities = 75/113 (66%), Positives = 80/113 (70%), Gaps = 2/113 (1%)
 Frame = +2

Query: 251 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRE--RYQIEANSEY*M*RSSKRNEGK 424
           MPLEPRASWFSPKCVEAQQLT     K  F  G     + +   N+ Y       +  G 
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGCASGTKSRQTLNTRY-----DPKITGV 55

Query: 425 GSTSETMRDKLHRREGNSPDHQLRPLNDRSVVKEVGVQRQPGGLPRSSHP*KS 583
            S SETM DKLHRREGNSPDHQLRPLNDRSV+KEVGVQRQPGGLPRSSHP KS
Sbjct: 56  RSASETMGDKLHRREGNSPDHQLRPLNDRSVIKEVGVQRQPGGLPRSSHPLKS 108


>tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medicago
           truncatula PE=4 SV=1
          Length = 102

 Score =  129 bits (323), Expect = 2e-28
 Identities = 70/108 (64%), Positives = 77/108 (71%)
 Frame = +2

Query: 251 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY*M*RSSKRNEGKGS 430
           MPLEPRASWFSPKCVEAQQLT     K  F  G       E+ ++     +++      S
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGR------ESGTKSRQTLNTRYPWRVKS 54

Query: 431 TSETMRDKLHRREGNSPDHQLRPLNDRSVVKEVGVQRQPGGLPRSSHP 574
            SE M DKLHRREGNSPDHQLRPLNDRSV+KEVGVQRQPGGLPRSSHP
Sbjct: 55  ASEAMGDKLHRREGNSPDHQLRPLNDRSVIKEVGVQRQPGGLPRSSHP 102


>tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whole
           genome shotgun sequence OS=Vitis vinifera
           GN=GSVIVT00035369001 PE=4 SV=1
          Length = 235

 Score = 77.0 bits (188), Expect(2) = 1e-19
 Identities = 37/41 (90%), Positives = 38/41 (92%)
 Frame = -1

Query: 312 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSVRTST 190
           VN CASTHFGENQLALGSS ISPLTTTHPLI QHQSV+TST
Sbjct: 57  VNRCASTHFGENQLALGSSDISPLTTTHPLILQHQSVQTST 97



 Score = 43.5 bits (101), Expect(2) = 1e-19
 Identities = 18/23 (78%), Positives = 22/23 (95%)
 Frame = -3

Query: 388 VFRVCLDLVPLSQPAPERCFTPR 320
           +FRVCLDLVPLS+PA ++CFTPR
Sbjct: 32  MFRVCLDLVPLSRPALKQCFTPR 54


>tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1
          Length = 46

 Score = 87.4 bits (215), Expect = 5e-16
 Identities = 42/46 (91%), Positives = 42/46 (91%)
 Frame = +2

Query: 251 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY 388
           MPLEPRASWFSPKCVEAQQLT PSRGKA FR G RERYQIEANSEY
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGPSRGKALFRYGPRERYQIEANSEY 46


>tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein
           OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_02439 PE=4
           SV=1
          Length = 199

 Score = 79.3 bits (194), Expect = 1e-13
 Identities = 43/80 (53%), Positives = 52/80 (65%)
 Frame = -2

Query: 326 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 147
           P  + + A+P+ IS RTS L VR+ F P P LI   FN   FGPPL  T AS+W+WIDHP
Sbjct: 11  PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 146 GSGL*AVTIAPARLAFATAP 87
            SGL  +T+A  RL F TAP
Sbjct: 71  VSGLPPLTLALLRLGFPTAP 90


>tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein
           OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_01293 PE=4
           SV=1
          Length = 199

 Score = 79.3 bits (194), Expect = 1e-13
 Identities = 43/80 (53%), Positives = 52/80 (65%)
 Frame = -2

Query: 326 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 147
           P  + + A+P+ IS RTS L VR+ F P P LI   FN   FGPPL  T AS+W+WIDHP
Sbjct: 11  PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 146 GSGL*AVTIAPARLAFATAP 87
            SGL  +T+A  RL F TAP
Sbjct: 71  VSGLPPLTLALLRLGFPTAP 90


>tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein
           OS=Ruminococcus torques ATCC 27756 GN=RUMTOR_00910 PE=4
           SV=1
          Length = 245

 Score = 76.6 bits (187), Expect = 9e-13
 Identities = 41/73 (56%), Positives = 48/73 (65%)
 Frame = -2

Query: 305 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 126
           A+P+ IS RTS L VR+ F P P LI   FN   FGPPL FT  S+W+WIDHP SGL  +
Sbjct: 4   ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 125 TIAPARLAFATAP 87
           T A  RL F +AP
Sbjct: 64  TFALFRLGFPSAP 76


>tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein
           OS=Ruminococcus gnavus ATCC 29149 GN=RUMGNA_03973 PE=4
           SV=1
          Length = 81

 Score = 71.6 bits (174), Expect(2) = 3e-12
 Identities = 37/64 (57%), Positives = 44/64 (68%)
 Frame = -2

Query: 305 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 126
           A+P+ IS RTS L VR+ F P P LI   FN   FGPPL FT  S+W+WIDHP SGL  +
Sbjct: 4   ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 125 TIAP 114
           TI+P
Sbjct: 64  TISP 67



 Score = 23.9 bits (50), Expect(2) = 3e-12
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = -3

Query: 118 PQQDSLSLRLRGFSP 74
           P  DS+SLRL+ FSP
Sbjct: 67  PCSDSVSLRLQAFSP 81


>tr|B1BC32|B1BC32_CLOBO Putative uncharacterized protein
           OS=Clostridium botulinum C str. Eklund GN=CBC_0457 PE=4
           SV=1
          Length = 93

 Score = 45.4 bits (106), Expect(2) = 4e-10
 Identities = 22/49 (44%), Positives = 29/49 (59%)
 Frame = -3

Query: 358 LSQPAPERCFTPRWSSQLLRLNAFRGEPASSGFEWHFTPNHNSSADFST 212
           +S P+P     P  +   L L  FRGEPA S F+W+F+  H+SS  FST
Sbjct: 1   MSAPSPFSALPPLLNYPTLALKLFRGEPAISEFDWNFSAIHSSSHGFST 49



 Score = 42.7 bits (99), Expect(2) = 4e-10
 Identities = 19/35 (54%), Positives = 21/35 (60%)
 Frame = -2

Query: 203 FGPPLSFTQASSWSWIDHPGSGL*AVTIAPARLAF 99
           FGPP +FT ASSW WI H  SGL   T  P +  F
Sbjct: 53  FGPPRNFTSASSWPWIGHSVSGLQYATSCPVQTRF 87