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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK953133
Clone id TST38A01NGRL0015_P12
Library
Length 658
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0015_P12. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence 
GCCTCGGCTATGGCTTCGAGGGTCTCGTCCTCTCCGCAGTGAAGGCGCCCCCTTGTCTCA
AAGCTCGGCTGCAGGTATTCCCTTTGTTGTGGGTTTGATTTTCGCGCAGATTTGCTTTTG
GGCTTCCTGCAAACACCTTCTTTTTGCCGCGGATCTCTGTCAGACCTCGCAAAGCTCAAG
GTCTGTTTTAGGGCATTTGATACATGCTGGATATGGTTATGGAGATGATAACCGGGGCAG
ATCCTTACACTCTAGCTGCGGTGGGGGCCTCGTTGATTGTTGCAGTAGGCATCTACTATC
TGGTTTTCATTCTGGGCCCTGCCGAAACAAAGGGCAAATCTGCAGTAGAGCTGGGAGGGG
GCTCTCTTGCACGGGAGCAGGTCGCAGATAACTACAAGGCCTATGCAGATTTTTTCAAAA
AGCCTAAGGAGATTGAAAATGCAAAACAGGTTCCAAAACTTGTAGACACCTTCTACAACC
TTGTGACTGACATCTATGAATGGGGCTGGGGACAGAGCTTTCATTTTTCACCTGCAATTA
CAGGAAAATCCCATCGTGAGGAGACTAGACTTCATGAGGAACACGTTGCAGATCTTCTGC
ACATTAAGCCCAAGGACAAGGTTCTTGATGCAGGTTGTGGTGTGGGGGGCCCGATGCG
■■Homology search results ■■ -
sp_hit_id Q39227
Definition sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana
Align length 139
Score (bit) 197.0
E-value 3.0e-50
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953133|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0015_P12, 5'
         (658 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2 OS...   197   3e-50
sp|Q94JS4|SMT3B_ARATH 24-methylenesterol C-methyltransferase 3 O...   183   8e-46
sp|O82427|SMT2_ORYSJ 24-methylenesterol C-methyltransferase 2 OS...   170   7e-42
sp|Q6ZIX2|SMT1_ORYSJ Cycloartenol-C-24-methyltransferase 1 OS=Or...    85   4e-16
sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase OS=Arab...    82   3e-15
sp|Q6BRB7|ERG6_DEBHA Sterol 24-C-methyltransferase OS=Debaryomyc...    81   4e-15
sp|Q6C2D9|ERG6_YARLI Sterol 24-C-methyltransferase OS=Yarrowia l...    80   7e-15
sp|O74198|ERG6_CANAL Sterol 24-C-methyltransferase OS=Candida al...    79   2e-14
sp|Q54I98|SMT1_DICDI Probable cycloartenol-C-24-methyltransferas...    78   4e-14
sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase OS=Schizosacc...    78   4e-14
sp|Q875K1|ERG6_CLALS Sterol 24-C-methyltransferase OS=Clavispora...    77   6e-14
sp|Q9P3R1|ERG6_NEUCR Sterol 24-C-methyltransferase OS=Neurospora...    77   1e-13
sp|Q6FRZ7|ERG6_CANGA Sterol 24-C-methyltransferase OS=Candida gl...    76   1e-13
sp|Q6CYB3|ERG6_KLULA Sterol 24-C-methyltransferase OS=Kluyveromy...    75   4e-13
sp|P25087|ERG6_YEAST Sterol 24-C-methyltransferase OS=Saccharomy...    74   9e-13
sp|Q759S7|ERG6_ASHGO Sterol 24-C-methyltransferase OS=Ashbya gos...    72   2e-12
sp|Q5EN22|ERG6_MAGGR Sterol 24-C-methyltransferase OS=Magnaporth...    72   3e-12
sp|Q96WX4|ERG6_PNECA Sterol 24-C-methyltransferase OS=Pneumocyst...    65   3e-10
sp|Q9ZSK1|GTOMC_ARATH Tocopherol O-methyltransferase, chloroplas...    36   0.20 
sp|Q6LMS7|RUMA_PHOPR 23S rRNA (uracil-5-)-methyltransferase rumA...    35   0.35 
sp|Q9C6B9|PEAM3_ARATH Putative phosphoethanolamine N-methyltrans...    35   0.45 
sp|P31695|NOTC4_MOUSE Neurogenic locus notch homolog protein 4 O...    33   1.0  
sp|Q84BQ9|PRMA_THET8 Ribosomal protein L11 methyltransferase OS=...    32   2.9  
sp|P59051|CU087_HUMAN Uncharacterized protein C21orf87 OS=Homo s...    32   2.9  
sp|Q8E6W1|Y448_STRA3 Uncharacterized RNA methyltransferase gbs04...    32   3.8  
sp|Q8E1E4|Y413_STRA5 Uncharacterized RNA methyltransferase SAG04...    32   3.8  
sp|Q9M571|PEAMT_SPIOL Phosphoethanolamine N-methyltransferase OS...    32   3.8  
sp|Q9FR44|PEAM1_ARATH Phosphoethanolamine N-methyltransferase 1 ...    32   3.8  
sp|Q8VEJ3|DKK4_MOUSE Dickkopf-related protein 4 OS=Mus musculus ...    32   3.9  
sp|O74885|YQE1_SCHPO Putative dioxygenase C576.01c OS=Schizosacc...    31   6.5  

>sp|Q39227|SMT2_ARATH 24-methylenesterol C-methyltransferase 2
           OS=Arabidopsis thaliana GN=SMT2 PE=1 SV=2
          Length = 361

 Score =  197 bits (502), Expect = 3e-50
 Identities = 92/139 (66%), Positives = 110/139 (79%)
 Frame = +3

Query: 240 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 419
           D  TL   GA  +VAVGIY+ + +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DSLTLFFTGA--LVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59

Query: 420 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 599
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH++ TRLHEE   DL+
Sbjct: 60  RPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLI 119

Query: 600 HIKPKDKVLDAGCGVGGPM 656
            +KP  K+LD GCGVGGPM
Sbjct: 120 QVKPGQKILDVGCGVGGPM 138


>sp|Q94JS4|SMT3B_ARATH 24-methylenesterol C-methyltransferase 3
           OS=Arabidopsis thaliana GN=SMT3 PE=2 SV=1
          Length = 359

 Score =  183 bits (464), Expect = 8e-46
 Identities = 80/127 (62%), Positives = 101/127 (79%)
 Frame = +3

Query: 276 IVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVP 455
           ++A  +Y+ + +LGPAE KGK A +L GGS++ E+V DNY  Y  FF+KPKEIE+A++VP
Sbjct: 12  LIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRKPKEIESAEKVP 71

Query: 456 KLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAG 635
             VDTFYNLVTDIYEWGWGQSFHFSP + GKS ++ TR+HEE   DL+ +KP  K+LDAG
Sbjct: 72  DFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAG 131

Query: 636 CGVGGPM 656
           CGVGGPM
Sbjct: 132 CGVGGPM 138


>sp|O82427|SMT2_ORYSJ 24-methylenesterol C-methyltransferase 2
           OS=Oryza sativa subsp. japonica GN=Smt2-1 PE=2 SV=2
          Length = 363

 Score =  170 bits (430), Expect = 7e-42
 Identities = 85/150 (56%), Positives = 107/150 (71%), Gaps = 4/150 (2%)
 Frame = +3

Query: 219 MEMITGADPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYK 398
           ME  T A  +T A VG +L+     Y+ V+++G AE KGK AV+L  GS+  ++V D Y 
Sbjct: 1   MEAATMA--WTAAGVGMALV-----YWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYT 53

Query: 399 AYADFFKKPKEIE----NAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREET 566
            Y  FF++PKE      +A++VP  VDTFYNLVTDIYEWGWGQSFHFSP++ G+SHRE T
Sbjct: 54  QYWSFFRRPKETATTEASAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREAT 113

Query: 567 RLHEEHVADLLHIKPKDKVLDAGCGVGGPM 656
           R+HEE VADLL  KP  ++LD GCGVGGPM
Sbjct: 114 RVHEERVADLLQAKPGHRLLDVGCGVGGPM 143


>sp|Q6ZIX2|SMT1_ORYSJ Cycloartenol-C-24-methyltransferase 1 OS=Oryza
           sativa subsp. japonica GN=Smt1-1 PE=2 SV=1
          Length = 344

 Score = 84.7 bits (208), Expect = 4e-16
 Identities = 40/103 (38%), Positives = 62/103 (60%), Gaps = 3/103 (2%)
 Frame = +3

Query: 357 GGSLAREQV---ADNYKAYADFFKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHF 527
           GG + +++V    D Y+ Y  ++   +E   +     +V+ +Y+L T  YE+GWG+SFHF
Sbjct: 14  GGKITKDEVKSAVDEYEKYHGYYGGKEEARKSNYTD-MVNKYYDLATSFYEYGWGESFHF 72

Query: 528 SPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPM 656
           +    G+S RE  + HE  +A  L +KP  KVLD GCG+GGP+
Sbjct: 73  AHRWNGESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPL 115


>sp|Q9LM02|SMT1_ARATH Cycloartenol-C-24-methyltransferase
           OS=Arabidopsis thaliana GN=SMT1 PE=2 SV=1
          Length = 336

 Score = 81.6 bits (200), Expect = 3e-15
 Identities = 40/102 (39%), Positives = 57/102 (55%), Gaps = 2/102 (1%)
 Frame = +3

Query: 357 GGSLAREQVADNYKAYADF--FKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFS 530
           GG + +  V    + Y  +  F    E E       +V+ +Y+L T  YE+GWG+SFHF+
Sbjct: 8   GGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFA 67

Query: 531 PAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPM 656
               G+S RE  + HE  +A  L I+P  KVLD GCG+GGP+
Sbjct: 68  QRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPL 109


>sp|Q6BRB7|ERG6_DEBHA Sterol 24-C-methyltransferase OS=Debaryomyces
           hansenii GN=ERG6 PE=3 SV=1
          Length = 377

 Score = 81.3 bits (199), Expect = 4e-15
 Identities = 43/96 (44%), Positives = 52/96 (54%), Gaps = 1/96 (1%)
 Frame = +3

Query: 369 AREQVADNY-KAYADFFKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITG 545
           A E   D Y K +     K  E +       L   +YNLVTD YE+GWG SFHFS   TG
Sbjct: 42  ASEVANDGYFKHWDGGVTKEDEDKRLSDYSNLTAHYYNLVTDFYEYGWGSSFHFSRYYTG 101

Query: 546 KSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGP 653
           ++ R+ T  HE  +A  + I    KVLD GCGVGGP
Sbjct: 102 EAFRQATARHEHFLAHKMQINENMKVLDVGCGVGGP 137


>sp|Q6C2D9|ERG6_YARLI Sterol 24-C-methyltransferase OS=Yarrowia
           lipolytica GN=ERG6 PE=3 SV=1
          Length = 381

 Score = 80.5 bits (197), Expect = 7e-15
 Identities = 40/85 (47%), Positives = 54/85 (63%), Gaps = 6/85 (7%)
 Frame = +3

Query: 417 KKPKEIENAKQVPKLVDT------FYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHE 578
           K   +IE+A +  ++ D       +YNLVTD YE+GWG SFHFS    G++ R+ T  HE
Sbjct: 58  KDRSKIEDADEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHE 117

Query: 579 EHVADLLHIKPKDKVLDAGCGVGGP 653
            ++A  + I+P  KVLD GCGVGGP
Sbjct: 118 HYLAYKMGIQPGMKVLDVGCGVGGP 142


>sp|O74198|ERG6_CANAL Sterol 24-C-methyltransferase OS=Candida
           albicans GN=ERG6 PE=3 SV=1
          Length = 376

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 37/87 (42%), Positives = 50/87 (57%)
 Frame = +3

Query: 393 YKAYADFFKKPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRL 572
           +K +     K  E +      +L   +YNLVTD YE+GWG SFHFS    G++ R+ T  
Sbjct: 50  FKHWDGGISKDDEEKRLNDYSQLTHHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATAR 109

Query: 573 HEEHVADLLHIKPKDKVLDAGCGVGGP 653
           HE  +A  +++    KVLD GCGVGGP
Sbjct: 110 HEHFLAHKMNLNENMKVLDVGCGVGGP 136


>sp|Q54I98|SMT1_DICDI Probable cycloartenol-C-24-methyltransferase 1
           OS=Dictyostelium discoideum GN=smt1 PE=1 SV=1
          Length = 354

 Score = 78.2 bits (191), Expect = 4e-14
 Identities = 42/97 (43%), Positives = 56/97 (57%), Gaps = 3/97 (3%)
 Frame = +3

Query: 375 EQVADNYKAYADFFK--KPKEIENAKQ-VPKLVDTFYNLVTDIYEWGWGQSFHFSPAITG 545
           ++V D    Y + FK    K I+  K     +V+TFY+L TD YE+GWGQSFHF+     
Sbjct: 30  DKVDDTLNGYKELFKGNDDKAIQARKNNYTHMVNTFYDLATDFYEFGWGQSFHFATRHKY 89

Query: 546 KSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGPM 656
           +S       HE ++A  L + P  KV+D GCGVGGPM
Sbjct: 90  ESFEASIARHEMYMAHQLGLFPGMKVIDIGCGVGGPM 126


>sp|O14321|ERG6_SCHPO Sterol 24-C-methyltransferase
           OS=Schizosaccharomyces pombe GN=SPBC16E9.05 PE=2 SV=1
          Length = 378

 Score = 78.2 bits (191), Expect = 4e-14
 Identities = 41/118 (34%), Positives = 69/118 (58%), Gaps = 11/118 (9%)
 Frame = +3

Query: 333 GKSAVELGG-GSLAREQVADNYKAYADFFK-------KPKEIENAKQVP---KLVDTFYN 479
           GK+A +  G  ++A + V +  +   ++F+          E + A+++     +V+++Y+
Sbjct: 21  GKAAEKKTGLAAIASKDVDEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVVNSYYD 80

Query: 480 LVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGCGVGGP 653
           L TD+YE+GW QSFHFS    G++  +    HE ++A  + IKP  +VLD GCGVGGP
Sbjct: 81  LATDLYEYGWSQSFHFSRFYKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGP 138
tr_hit_id B8LK59
Definition tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 133
Score (bit) 213.0
E-value 1.0e-53
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953133|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0015_P12, 5'
         (658 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea...   213   1e-53
tr|B0FDL8|B0FDL8_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...   198   3e-49
tr|B0FDL7|B0FDL7_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...   195   2e-48
tr|A9P9Q4|A9P9Q4_POPTR Putative uncharacterized protein OS=Popul...   195   2e-48
tr|A7P8G6|A7P8G6_VITVI Chromosome chr3 scaffold_8, whole genome ...   192   1e-47
tr|O24154|O24154_TOBAC S-adenosyl-methionine-sterol-C-methyltran...   192   2e-47
tr|A9PE86|A9PE86_POPTR Putative uncharacterized protein OS=Popul...   191   3e-47
tr|A9T0X1|A9T0X1_PHYPA Predicted protein OS=Physcomitrella paten...   189   1e-46
tr|A9T0U8|A9T0U8_PHYPA Predicted protein OS=Physcomitrella paten...   189   1e-46
tr|O24153|O24153_TOBAC S-adenosyl-methionine-sterol-C-methyltran...   188   3e-46
tr|A9TCR1|A9TCR1_PHYPA Predicted protein OS=Physcomitrella paten...   186   8e-46
tr|A7NVP2|A7NVP2_VITVI Chromosome chr18 scaffold_1, whole genome...   186   8e-46
tr|A5BH10|A5BH10_VITVI Putative uncharacterized protein OS=Vitis...   183   7e-45
tr|B4FQZ7|B4FQZ7_MAIZE Putative uncharacterized protein OS=Zea m...   170   6e-41
tr|B4FC47|B4FC47_MAIZE Putative uncharacterized protein OS=Zea m...   170   6e-41
tr|B4FB47|B4FB47_MAIZE Putative uncharacterized protein OS=Zea m...   170   6e-41
tr|Q10S41|Q10S41_ORYSJ Os03g0136200 protein OS=Oryza sativa subs...   170   8e-41
tr|A3ADV8|A3ADV8_ORYSJ Putative uncharacterized protein OS=Oryza...   169   1e-40
tr|A2XC88|A2XC88_ORYSI Putative uncharacterized protein OS=Oryza...   169   1e-40
tr|B6TB41|B6TB41_MAIZE 24-methylenesterol C-methyltransferase 2 ...   168   2e-40
tr|B6TJP4|B6TJP4_MAIZE 24-methylenesterol C-methyltransferase 2 ...   167   4e-40
tr|A8IJ34|A8IJ34_CHLRE Sterol-C24-methyltransferase OS=Chlamydom...   115   2e-24
tr|A4RUR2|A4RUR2_OSTLU Predicted protein OS=Ostreococcus lucimar...    95   3e-18
tr|O82720|O82720_TOBAC S-adenosyl-methionine-sterol-C-methyltran...    88   4e-16
tr|A7Q258|A7Q258_VITVI Chromosome chr13 scaffold_45, whole genom...    87   7e-16
tr|Q2QDF7|Q2QDF7_GOSHI 24-sterol C-methyltransferase OS=Gossypiu...    87   1e-15
tr|O24328|O24328_RICCO S-adenosyl-methionine-sterol-C-methyltran...    86   3e-15
tr|B8BTY9|B8BTY9_THAPS Predicted protein (Fragment) OS=Thalassio...    86   3e-15
tr|A9V6Y8|A9V6Y8_MONBE Predicted protein OS=Monosiga brevicollis...    86   3e-15
tr|Q0D7V5|Q0D7V5_ORYSJ Os07g0206700 protein OS=Oryza sativa subs...    85   4e-15

>tr|B8LK59|B8LK59_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 369

 Score =  213 bits (541), Expect = 1e-53
 Identities = 98/133 (73%), Positives = 118/133 (88%)
 Frame = +3

Query: 258 AVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIE 437
           AVG + ++ +G+Y++++++G AE +GK AVELGGGSL+R QVADNYK Y+ FF+KPKEIE
Sbjct: 10  AVGTA-VLGMGLYWILYVVGAAEQRGKRAVELGGGSLSRNQVADNYKQYSAFFEKPKEIE 68

Query: 438 NAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKD 617
            A +VPKLVDTFYNLVTDIYEWGWGQSFHFSPA+ GKSH+EETRLHEE VADLL +KP D
Sbjct: 69  KADKVPKLVDTFYNLVTDIYEWGWGQSFHFSPALPGKSHKEETRLHEEFVADLLKLKPGD 128

Query: 618 KVLDAGCGVGGPM 656
           +VLDAGCGVGGPM
Sbjct: 129 RVLDAGCGVGGPM 141


>tr|B0FDL8|B0FDL8_GOSHI 24-sterol C-methyltransferase OS=Gossypium
           hirsutum GN=SMT2-2 PE=2 SV=1
          Length = 361

 Score =  198 bits (503), Expect = 3e-49
 Identities = 92/139 (66%), Positives = 110/139 (79%)
 Frame = +3

Query: 240 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 419
           DP  L   GA  ++A G+Y+ V +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DPVGLFCTGA--LLACGLYWFVCLLGPAEQKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59

Query: 420 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 599
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHR+ TRLHEE   DL+
Sbjct: 60  QPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIRGKSHRDATRLHEEMAVDLI 119

Query: 600 HIKPKDKVLDAGCGVGGPM 656
            +KP D+VLD GCGVGGPM
Sbjct: 120 EVKPGDRVLDVGCGVGGPM 138


>tr|B0FDL7|B0FDL7_GOSHI 24-sterol C-methyltransferase OS=Gossypium
           hirsutum GN=SMT2-1 PE=2 SV=1
          Length = 361

 Score =  195 bits (496), Expect = 2e-48
 Identities = 90/139 (64%), Positives = 110/139 (79%)
 Frame = +3

Query: 240 DPYTLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFK 419
           D  +L   GA  ++A G+Y+ V +LGPAE KGK AV+L GGS++ E+V DNYK Y  FF+
Sbjct: 2   DSLSLFCTGA--LLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFR 59

Query: 420 KPKEIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLL 599
           +PKEIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHR+ TRLHEE   DL+
Sbjct: 60  RPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLI 119

Query: 600 HIKPKDKVLDAGCGVGGPM 656
            +KP D++LD GCGVGGPM
Sbjct: 120 DVKPGDRILDVGCGVGGPM 138


>tr|A9P9Q4|A9P9Q4_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 364

 Score =  195 bits (496), Expect = 2e-48
 Identities = 90/136 (66%), Positives = 107/136 (78%)
 Frame = +3

Query: 249 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 428
           +LA      ++A GIY+ V ILGPAE KGK AV+L GGS++ E+V DNYK Y  FF+ PK
Sbjct: 3   SLALFCTGALIAGGIYWFVCILGPAEQKGKRAVDLSGGSISAEKVQDNYKQYWSFFRSPK 62

Query: 429 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 608
           EIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH E TRLHEE   DL+++K
Sbjct: 63  EIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHLEATRLHEEMAVDLINVK 122

Query: 609 PKDKVLDAGCGVGGPM 656
           P D++LD GCGVGGPM
Sbjct: 123 PGDRILDVGCGVGGPM 138


>tr|A7P8G6|A7P8G6_VITVI Chromosome chr3 scaffold_8, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00036172001
           PE=4 SV=1
          Length = 356

 Score =  192 bits (489), Expect = 1e-47
 Identities = 89/136 (65%), Positives = 109/136 (80%)
 Frame = +3

Query: 249 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 428
           +LA    + +VA G+Y+ VF+LG AE KGK AV L GGS++ ++V DNYK Y  FF++PK
Sbjct: 3   SLALFCTASLVAGGVYWFVFLLGAAERKGKRAVHLSGGSISGDKVRDNYKQYWSFFRRPK 62

Query: 429 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 608
           EIE A++VP  VDTFYNLVTDIYEWGWGQSFHFSP++ GKSHR+ TR+HEE  ADLL I+
Sbjct: 63  EIETAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSVAGKSHRDATRVHEEMAADLLGIR 122

Query: 609 PKDKVLDAGCGVGGPM 656
             D+VLDAGCGVGGPM
Sbjct: 123 AGDRVLDAGCGVGGPM 138


>tr|O24154|O24154_TOBAC
           S-adenosyl-methionine-sterol-C-methyltransferase
           OS=Nicotiana tabacum PE=2 SV=1
          Length = 357

 Score =  192 bits (488), Expect = 2e-47
 Identities = 88/130 (67%), Positives = 104/130 (80%)
 Frame = +3

Query: 267 ASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAK 446
           A+L+ A G+Y+ V ILG AE KGK AV+L GGS+ +E V DNYK Y  FF++PKEIE A 
Sbjct: 10  AALLAAGGLYWFVCILGSAEVKGKRAVQLSGGSIEKENVQDNYKQYWSFFRRPKEIETAD 69

Query: 447 QVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVL 626
           +VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSHRE TR+HEE   DL+ +KP  ++L
Sbjct: 70  KVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHREATRIHEEMAVDLIGVKPGARIL 129

Query: 627 DAGCGVGGPM 656
           DAGCGVGGPM
Sbjct: 130 DAGCGVGGPM 139


>tr|A9PE86|A9PE86_POPTR Putative uncharacterized protein OS=Populus
           trichocarpa PE=2 SV=1
          Length = 364

 Score =  191 bits (485), Expect = 3e-47
 Identities = 87/136 (63%), Positives = 105/136 (77%)
 Frame = +3

Query: 249 TLAAVGASLIVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPK 428
           +LA      ++A GIY+ V +LGPAE KGK AV+L GGS++ E V DNY  Y  FF++PK
Sbjct: 3   SLALFFTGALLAGGIYWFVCVLGPAEQKGKRAVDLSGGSISAENVQDNYDQYWSFFRRPK 62

Query: 429 EIENAKQVPKLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIK 608
           EIE  ++VP  VDTFYNLVTDIYEWGWGQSFHFSP+I GKSH E TRLHEE   DL+++K
Sbjct: 63  EIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHSEATRLHEEMAVDLINVK 122

Query: 609 PKDKVLDAGCGVGGPM 656
           P D++LD GCGVGGPM
Sbjct: 123 PGDRILDVGCGVGGPM 138


>tr|A9T0X1|A9T0X1_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_86618 PE=4 SV=1
          Length = 349

 Score =  189 bits (481), Expect = 1e-46
 Identities = 86/126 (68%), Positives = 99/126 (78%)
 Frame = +3

Query: 279 VAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVPK 458
           +  G YY++ + G AE KGK AV+LGGGS+ REQV  +Y+ YA FF+KPK+IE    VP 
Sbjct: 4   LGAGAYYVLVLAGAAERKGKRAVDLGGGSITREQVRSSYQKYASFFEKPKQIEAKDNVPN 63

Query: 459 LVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGC 638
            VDTFYNLVTDIYEWGWGQSFHFSPA+ GKSH+E TRLHEE V DLL +KP  KVLD GC
Sbjct: 64  FVDTFYNLVTDIYEWGWGQSFHFSPAVPGKSHKESTRLHEERVVDLLGVKPGQKVLDVGC 123

Query: 639 GVGGPM 656
           GVGGPM
Sbjct: 124 GVGGPM 129


>tr|A9T0U8|A9T0U8_PHYPA Predicted protein OS=Physcomitrella patens
           subsp. patens GN=PHYPADRAFT_231984 PE=4 SV=1
          Length = 346

 Score =  189 bits (480), Expect = 1e-46
 Identities = 85/126 (67%), Positives = 100/126 (79%)
 Frame = +3

Query: 279 VAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVPK 458
           +  G YY++ + G AE KGK AV+LGGGS+ REQV  +Y+ YA FF+KPK+IE    VP 
Sbjct: 1   MGAGAYYMLVMTGAAERKGKRAVDLGGGSITREQVKSSYQKYASFFEKPKQIEAKDNVPN 60

Query: 459 LVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAGC 638
            VDTFYNLVTDIYEWGWGQSFHFSPA+ GKSH+E TR+HEE V DLL +KP +KVLD GC
Sbjct: 61  FVDTFYNLVTDIYEWGWGQSFHFSPAVPGKSHKESTRIHEERVVDLLGLKPGEKVLDVGC 120

Query: 639 GVGGPM 656
           GVGGPM
Sbjct: 121 GVGGPM 126


>tr|O24153|O24153_TOBAC
           S-adenosyl-methionine-sterol-C-methyltransferase homolog
           (Fragment) OS=Nicotiana tabacum PE=2 SV=1
          Length = 352

 Score =  188 bits (477), Expect = 3e-46
 Identities = 89/127 (70%), Positives = 100/127 (78%)
 Frame = +3

Query: 276 IVAVGIYYLVFILGPAETKGKSAVELGGGSLAREQVADNYKAYADFFKKPKEIENAKQVP 455
           ++A G+Y+ V ILG AE KGK AV L GG +A+E+V D YK Y  FF++PKEIE  + VP
Sbjct: 7   LLAGGLYWFVCILGSAEIKGKRAVNLSGGFIAKEKVQDKYKQYWSFFRRPKEIETTENVP 66

Query: 456 KLVDTFYNLVTDIYEWGWGQSFHFSPAITGKSHREETRLHEEHVADLLHIKPKDKVLDAG 635
             VDTFYNLVTDIYEWGWGQSFHFSPAITGK HRE TRLHEE   DLL IKP  +VLDAG
Sbjct: 67  AFVDTFYNLVTDIYEWGWGQSFHFSPAITGKFHREATRLHEEMAVDLLGIKPGARVLDAG 126

Query: 636 CGVGGPM 656
           CGVGGPM
Sbjct: 127 CGVGGPM 133