DK953212
Clone id TST39A01NGRL0017_C21
Library
Length 529
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_C21. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CACAGAGGCACGCCATCGCCGCGAGCTGGTTTGGGGTCTCTGACGATGGCTTGCAGCAGC
GGCAACAGAGCCATGCAGGCGCCCTCTTCTCTCTCCTCCTGCGAGGCCTCCTCTCTGCGT
GGCCTCTCTTTGGCTCCTGCCTTGAGGCCCCGAGCTCGATCGCCGCCTTCTTCCTCTGCT
CCTCATCACACTCTGAAGGTCATGGCTATGGCGCCCAAAAACAAGGTGAATGCATATGAT
TCTGATTGGAACAAAACATGGTTCGGAGCCGGGATGTTTGCCGAAAATACAGAGGCTGCA
CCTATGAACATTGTGCAGAAGCTGGAGAAGAAGAAGCTTCTTAGCCAAGTAGAGAAGGCA
GGGCTTCTTTCAAAGGCAGAGAGTGCTGGCCTCACTCTCTCCTCAATAGAGAAGATGGGT
TTGTTGTCGAAGGCCGAGGATCTTGGTCTGTTGAGCTTTGCAGAGAGCTTCGCAACATCA
TCACCTGCACTTCTCGCATCTCTGTCATTGCCTCTCATTGTCCTATCTG
■■Homology search results ■■ -
sp_hit_id O74324
Definition sp|O74324|YH04_SCHPO Uncharacterized protein C1685.04 OS=Schizosaccharomyces pombe
Align length 54
Score (bit) 32.0
E-value 1.9
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK953212|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_C21, 5'
(529 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|O74324|YH04_SCHPO Uncharacterized protein C1685.04 OS=Schizos... 32 1.9
sp|O66976|HISX_AQUAE Histidinol dehydrogenase OS=Aquifex aeolicu... 32 2.5
sp|A1T041|RL9_PSYIN 50S ribosomal protein L9 OS=Psychromonas ing... 31 4.2
sp|Q66GS9|CP135_HUMAN Centrosomal protein of 135 kDa OS=Homo sap... 31 4.2
sp|Q9LS42|CASP_ARATH Protein CASP OS=Arabidopsis thaliana GN=CAS... 31 4.2
sp|Q32539|NU5C_LACSA NAD(P)H-quinone oxidoreductase subunit 5, c... 30 9.2
sp|O13817|SEC7C_SCHPO Protein transport protein sec73 OS=Schizos... 30 9.4
sp|Q5WFQ1|HSLU_BACSK ATP-dependent hsl protease ATP-binding subu... 30 9.4

>sp|O74324|YH04_SCHPO Uncharacterized protein C1685.04
OS=Schizosaccharomyces pombe GN=SPBC1685.04 PE=2 SV=1
Length = 325

Score = 32.0 bits (71), Expect = 1.9
Identities = 21/54 (38%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +1

Query: 286 NTEAAPMNIVQKLEKKKLLSQVEKAGLLSKAESAGLTLSSIEK-MGLLSKAEDL 444
NT + LEKKK LS ++ L + A +SS+EK M +L K EDL
Sbjct: 167 NTLVKRLQTYDNLEKKKALSMEDRLLTLQEQYDAHANVSSLEKRMEVLKKREDL 220


>sp|O66976|HISX_AQUAE Histidinol dehydrogenase OS=Aquifex aeolicus
GN=hisD PE=3 SV=1
Length = 426

Score = 31.6 bits (70), Expect = 2.5
Identities = 26/85 (30%), Positives = 39/85 (45%), Gaps = 4/85 (4%)
Frame = +1

Query: 202 MAMAPKNKVNAYDSDWNKTWFGAGMF--AENTEAAPMNIVQKLEK--KKLLSQVEKAGLL 369
M P + D N TW A M AE+ E A ++ E+ K+ +VE+ LL
Sbjct: 228 MIAGPSEVLVIADERANPTWVAADMLSQAEHDELAASILLTPSEELANKVKEEVER--LL 285

Query: 370 SKAESAGLTLSSIEKMGLLSKAEDL 444
S+ E + S+EK G + +DL
Sbjct: 286 SQLERKEIAQKSLEKFGTIFLVKDL 310


>sp|A1T041|RL9_PSYIN 50S ribosomal protein L9 OS=Psychromonas
ingrahamii (strain 37) GN=rplI PE=3 SV=1
Length = 150

Score = 30.8 bits (68), Expect = 4.2
Identities = 18/69 (26%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +1

Query: 253 KTWFGAGMFAENTEAAPMNI--VQKLEKKKLLSQVEKAGLLSKAESAGLTLSSIEKMGLL 426
K+ + +A P N ++ E +++ + ++A L+ AE G L+++E + +
Sbjct: 22 KSGYARNFLFPQQKAVPANKANLEAFETRRVELEAKQAANLAAAEKRGAALAALEAVVIA 81

Query: 427 SKAEDLGLL 453
SKA D+G L
Sbjct: 82 SKAGDVGKL 90


>sp|Q66GS9|CP135_HUMAN Centrosomal protein of 135 kDa OS=Homo
sapiens GN=CEP135 PE=1 SV=1
Length = 1140

Score = 30.8 bits (68), Expect = 4.2
Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +1

Query: 283 ENTEA-APMNIVQKLEKKKLLSQVEKAGLLSKAESAGLTLSSIEKMGLLSKAE 438
E+T+ AP NIV +EK+K L+ + ++++ E+ L IE++ L K+E
Sbjct: 553 ESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSE 605


>sp|Q9LS42|CASP_ARATH Protein CASP OS=Arabidopsis thaliana GN=CASP
PE=1 SV=2
Length = 689

Score = 30.8 bits (68), Expect = 4.2
Identities = 27/86 (31%), Positives = 39/86 (45%), Gaps = 3/86 (3%)
Frame = +1

Query: 193 LKVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQV-EKAGLL 369
+K++ N + A DW+ G M + N + E +L Q+ EKA LL
Sbjct: 380 VKILQAVGYNSIEA--EDWDAATTGEEMSKMESLLLDKNRKMEHEVTQLKVQLSEKASLL 437

Query: 370 SKAESAG--LTLSSIEKMGLLSKAED 441
KAE+ G LT E+ L+ K ED
Sbjct: 438 EKAEAKGEELTAKVNEQQRLIQKLED 463


>sp|Q32539|NU5C_LACSA NAD(P)H-quinone oxidoreductase subunit 5,
chloroplastic OS=Lactuca sativa GN=ndhF PE=3 SV=2
Length = 742

Score = 29.6 bits (65), Expect = 9.2
Identities = 27/95 (28%), Positives = 37/95 (38%), Gaps = 1/95 (1%)
Frame = -3

Query: 476 VAKLSAKLNRPRSSAFDNKPIFSIEERVRPALSAFERSPAFSTWLRSFFFSSFCTMFIGA 297
V L K N PR S F N ++ I+E VR + F P F G
Sbjct: 494 VTLLKMKKN-PRPSFFSNNKVYKIDENVRNMIQPFLSIPHF-----------------GN 535

Query: 296 ASVFSANIPAPNHVLFQ-SESYAFTLFLGAIAMTF 195
+S + N +LF FTLF+G + + F
Sbjct: 536 TKTYSYPYESDNTMLFPILILILFTLFVGFLGIPF 570


>sp|O13817|SEC7C_SCHPO Protein transport protein sec73
OS=Schizosaccharomyces pombe GN=sec73 PE=2 SV=2
Length = 1082

Score = 29.6 bits (65), Expect = 9.4
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = -1

Query: 466 SLQSSTDQDPRPSTTNPSSL 407
SL+SS D D RPS+T+PS+L
Sbjct: 902 SLESSNDIDDRPSSTSPSTL 921


>sp|Q5WFQ1|HSLU_BACSK ATP-dependent hsl protease ATP-binding subunit
hslU OS=Bacillus clausii (strain KSM-K16) GN=hslU PE=3
SV=1
Length = 463

Score = 29.6 bits (65), Expect = 9.4
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = +1

Query: 265 GAGM--FAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSKAESAGLTLSSIEKMGLLSKAE 438
GAGM N + N++ K +KK+ ++ E G+L++ E+ L +++AE
Sbjct: 210 GAGMEQMGMNMQEMLSNMMPKKKKKRRMTVAEARGVLAEEEAQKLIDMDDVTQEAITRAE 269

Query: 439 DLGLLSFAE 465
LG++ E
Sbjct: 270 QLGIIFIDE 278


tr_hit_id A9TMU4
Definition tr|A9TMU4|A9TMU4_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 90
Score (bit) 147.0
E-value 4.0e-34
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK953212|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_C21, 5'
(529 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9TMU4|A9TMU4_PHYPA Predicted protein OS=Physcomitrella paten... 147 4e-34
tr|A9TJ04|A9TJ04_PHYPA Predicted protein OS=Physcomitrella paten... 142 8e-33
tr|A9S8Z7|A9S8Z7_PHYPA Predicted protein (Fragment) OS=Physcomit... 133 5e-30
tr|A9NZ32|A9NZ32_PICSI Putative uncharacterized protein OS=Picea... 129 9e-29
tr|B6TFL9|B6TFL9_MAIZE Putative uncharacterized protein OS=Zea m... 129 1e-28
tr|B4FRC0|B4FRC0_MAIZE Putative uncharacterized protein OS=Zea m... 124 3e-27
tr|Q0JLN9|Q0JLN9_ORYSJ cDNA clone:J023103B06, full insert sequen... 123 7e-27
tr|B7FFF0|B7FFF0_MEDTR Putative uncharacterized protein OS=Medic... 123 7e-27
tr|A2WRV9|A2WRV9_ORYSI Putative uncharacterized protein OS=Oryza... 123 7e-27
tr|A5C347|A5C347_VITVI Putative uncharacterized protein (Chromos... 121 3e-26
tr|A9PJV1|A9PJV1_POPJC Putative uncharacterized protein OS=Popul... 119 1e-25
tr|Q9SSF7|Q9SSF7_ARATH F25A4.30 protein OS=Arabidopsis thaliana ... 119 1e-25
tr|Q94F10|Q94F10_ARATH Putative uncharacterized protein (Putativ... 119 1e-25
tr|B7FKD0|B7FKD0_MEDTR Putative uncharacterized protein OS=Medic... 117 5e-25
tr|A9PGF4|A9PGF4_POPTR Putative uncharacterized protein OS=Popul... 67 7e-10
tr|A3CIP9|A3CIP9_ORYSJ Putative uncharacterized protein OS=Oryza... 65 2e-09
tr|A7QI04|A7QI04_VITVI Chromosome chr17 scaffold_101, whole geno... 65 2e-09
tr|Q2QN86|Q2QN86_ORYSJ Os12g0575000 protein OS=Oryza sativa subs... 65 3e-09
tr|A9NMI1|A9NMI1_PICSI Putative uncharacterized protein OS=Picea... 65 3e-09
tr|B4FV96|B4FV96_MAIZE Putative uncharacterized protein OS=Zea m... 64 5e-09
tr|B8BMN2|B8BMN2_ORYSI Putative uncharacterized protein OS=Oryza... 64 6e-09
tr|A9S2G0|A9S2G0_PHYPA Predicted protein (Fragment) OS=Physcomit... 61 3e-08
tr|Q9SD79|Q9SD79_ARATH Putative uncharacterized protein F13G24.2... 61 4e-08
tr|Q8VYV1|Q8VYV1_ARATH AT5g08050/F13G24_250 OS=Arabidopsis thali... 61 4e-08
tr|Q2QN87|Q2QN87_ORYSJ Expressed protein OS=Oryza sativa subsp. ... 57 6e-07
tr|A2ZE10|A2ZE10_ORYSI Putative uncharacterized protein OS=Oryza... 47 6e-04
tr|Q2R4U2|Q2R4U2_ORYSJ Putative uncharacterized protein OS=Oryza... 47 8e-04
tr|B7G128|B7G128_PHATR Predicted protein OS=Phaeodactylum tricor... 44 0.007
tr|B8LBT9|B8LBT9_THAPS Predicted protein OS=Thalassiosira pseudo... 43 0.011
tr|A6R060|A6R060_AJECN Glycyl-tRNA synthetase 1 OS=Ajellomyces c... 35 0.52

>tr|A9TMU4|A9TMU4_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_147884 PE=4 SV=1
Length = 152

Score = 147 bits (370), Expect = 4e-34
Identities = 73/90 (81%), Positives = 80/90 (88%)
Frame = +1

Query: 208 MAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSKAESA 387
MAPK KVNAYD W+K WFGAG+FAENTE P+NIV+KLEKKKLLSQVEKAGLLSKAES
Sbjct: 1 MAPKKKVNAYDEGWSKQWFGAGIFAENTETEPVNIVKKLEKKKLLSQVEKAGLLSKAESL 60

Query: 388 GLTLSSIEKMGLLSKAEDLGLLSFAESFAT 477
G++LS IEK+GLLSKAEDLGLLS AE FAT
Sbjct: 61 GVSLSQIEKLGLLSKAEDLGLLSLAEKFAT 90


>tr|A9TJ04|A9TJ04_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_146224 PE=4 SV=1
Length = 165

Score = 142 bits (359), Expect = 8e-33
Identities = 71/94 (75%), Positives = 82/94 (87%)
Frame = +1

Query: 196 KVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSK 375
+V AMAPK KVNAYD W+K WFGAG+FAENTE ++IV+KLEKKKLLSQVEKAGLLSK
Sbjct: 10 QVRAMAPKKKVNAYDDAWSKQWFGAGLFAENTETESVDIVKKLEKKKLLSQVEKAGLLSK 69

Query: 376 AESAGLTLSSIEKMGLLSKAEDLGLLSFAESFAT 477
AES G++LS IEK+GLLSKAEDLGLL+ AE+F T
Sbjct: 70 AESLGVSLSQIEKLGLLSKAEDLGLLTLAENFVT 103


>tr|A9S8Z7|A9S8Z7_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_48633 PE=4 SV=1
Length = 117

Score = 133 bits (335), Expect = 5e-30
Identities = 69/94 (73%), Positives = 80/94 (85%)
Frame = +1

Query: 196 KVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSK 375
+V AMAPK KVNA+D W+K WFGAG+FAENTE ++IV+KLEKK LLSQVEKAGLLSK
Sbjct: 5 QVRAMAPKKKVNAHDDAWSKQWFGAGVFAENTETESVDIVKKLEKK-LLSQVEKAGLLSK 63

Query: 376 AESAGLTLSSIEKMGLLSKAEDLGLLSFAESFAT 477
AES G++LS IEK+GLLSKAEDLGLL+ AE F T
Sbjct: 64 AESLGVSLSQIEKLGLLSKAEDLGLLTLAEKFVT 97


>tr|A9NZ32|A9NZ32_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 209

Score = 129 bits (324), Expect = 9e-29
Identities = 64/95 (67%), Positives = 77/95 (81%)
Frame = +1

Query: 193 LKVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLS 372
L ++AMAPK KVN YD +W K WFGAG+F E E ++I++KLEK+K+LS VEKAGLLS
Sbjct: 54 LLILAMAPKKKVNKYDDNWKKQWFGAGIFLEGDEDVDVDIIKKLEKRKVLSGVEKAGLLS 113

Query: 373 KAESAGLTLSSIEKMGLLSKAEDLGLLSFAESFAT 477
KA+ GL+LSSIEKMGLLSKAEDLGLLS AE A+
Sbjct: 114 KADELGLSLSSIEKMGLLSKAEDLGLLSLAEKVAS 148


>tr|B6TFL9|B6TFL9_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 189

Score = 129 bits (323), Expect = 1e-28
Identities = 63/93 (67%), Positives = 76/93 (81%)
Frame = +1

Query: 196 KVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSK 375
KV AMAPK KVN YD W+K WFGAG+FAE +E +++ +KLE++K+LS VEKAGLLSK
Sbjct: 33 KVTAMAPKKKVNRYDESWSKQWFGAGIFAEGSEEVSVDVFKKLERRKVLSTVEKAGLLSK 92

Query: 376 AESAGLTLSSIEKMGLLSKAEDLGLLSFAESFA 474
AE GLTLSS+EK+GLLSKAEDLGLLS E+ A
Sbjct: 93 AEELGLTLSSLEKLGLLSKAEDLGLLSLVETAA 125


>tr|B4FRC0|B4FRC0_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 153

Score = 124 bits (311), Expect = 3e-27
Identities = 60/89 (67%), Positives = 73/89 (82%)
Frame = +1

Query: 208 MAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSKAESA 387
MAPK KVN YD W+K WFGAG+FAE +E +++ +KLE++K+LS VEKAGLLSKAE
Sbjct: 1 MAPKKKVNRYDESWSKQWFGAGIFAEGSEEVSVDVFKKLERRKVLSTVEKAGLLSKAEEL 60

Query: 388 GLTLSSIEKMGLLSKAEDLGLLSFAESFA 474
GLTLSS+EK+GLLSKAEDLGLLS E+ A
Sbjct: 61 GLTLSSLEKLGLLSKAEDLGLLSLVETAA 89


>tr|Q0JLN9|Q0JLN9_ORYSJ cDNA clone:J023103B06, full insert sequence
OS=Oryza sativa subsp. japonica GN=Os01g0585300 PE=2
SV=1
Length = 187

Score = 123 bits (308), Expect = 7e-27
Identities = 59/93 (63%), Positives = 76/93 (81%)
Frame = +1

Query: 196 KVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSK 375
KV AMAP+ KVN YD W+K WFGAG+FAE +E +++ +KLE++K+LS VEKAGLLS+
Sbjct: 31 KVTAMAPQKKVNRYDEKWSKQWFGAGIFAEGSEEVEVDVFKKLERRKVLSTVEKAGLLSR 90

Query: 376 AESAGLTLSSIEKMGLLSKAEDLGLLSFAESFA 474
AE G+TLSS+E++GLLSKAEDLGLLS E+ A
Sbjct: 91 AEELGVTLSSLEELGLLSKAEDLGLLSLVEAAA 123


>tr|B7FFF0|B7FFF0_MEDTR Putative uncharacterized protein OS=Medicago
truncatula PE=2 SV=1
Length = 193

Score = 123 bits (308), Expect = 7e-27
Identities = 60/92 (65%), Positives = 73/92 (79%)
Frame = +1

Query: 199 VMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSKA 378
V AMAPKNKVN Y+ +W K WFGAG+F E +E +++ +KLEK+K+LS VEKAGLLSKA
Sbjct: 38 VFAMAPKNKVNKYNDNWKKQWFGAGIFYEGSEEVEVDVFKKLEKRKVLSNVEKAGLLSKA 97

Query: 379 ESAGLTLSSIEKMGLLSKAEDLGLLSFAESFA 474
E G+TLSSIEK+GL SKAE+LGLLS E A
Sbjct: 98 EEFGVTLSSIEKLGLFSKAEELGLLSLLEKLA 129


>tr|A2WRV9|A2WRV9_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_02598 PE=4 SV=1
Length = 187

Score = 123 bits (308), Expect = 7e-27
Identities = 59/93 (63%), Positives = 76/93 (81%)
Frame = +1

Query: 196 KVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLSK 375
KV AMAP+ KVN YD W+K WFGAG+FAE +E +++ +KLE++K+LS VEKAGLLS+
Sbjct: 31 KVTAMAPQKKVNRYDEKWSKQWFGAGIFAEGSEEVEVDVFKKLERRKVLSTVEKAGLLSR 90

Query: 376 AESAGLTLSSIEKMGLLSKAEDLGLLSFAESFA 474
AE G+TLSS+E++GLLSKAEDLGLLS E+ A
Sbjct: 91 AEELGVTLSSLEELGLLSKAEDLGLLSLVEAAA 123


>tr|A5C347|A5C347_VITVI Putative uncharacterized protein (Chromosome
chr17 scaffold_101, whole genome shotgun sequence)
OS=Vitis vinifera GN=GSVIVT00000090001 PE=4 SV=1
Length = 193

Score = 121 bits (303), Expect = 3e-26
Identities = 58/95 (61%), Positives = 73/95 (76%)
Frame = +1

Query: 193 LKVMAMAPKNKVNAYDSDWNKTWFGAGMFAENTEAAPMNIVQKLEKKKLLSQVEKAGLLS 372
L+++AMA K KVN YD +W K WFGAG+F E +E +++ + LEKKK+LS VEK+GLLS
Sbjct: 36 LRILAMASKKKVNRYDENWKKEWFGAGIFVEGSEEVEVDVFKNLEKKKVLSNVEKSGLLS 95

Query: 373 KAESAGLTLSSIEKMGLLSKAEDLGLLSFAESFAT 477
KAE G TLSSIEK+G+ SKAEDLGLLS E A+
Sbjct: 96 KAEELGFTLSSIEKLGVFSKAEDLGLLSLLEKAAS 130