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NBDC - LSDB Archive
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DK953261
Clone id TST39A01NGRL0017_E23
Library
Length 578
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_E23. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
CGCCATGGCTAACTCCACCTCCATCGGCTCTCTCTTTCTCCCTCCAGAAGCCATCGACTA
CCACGGAGCCCTCTTGCTCGGCGCACTAACCCTCCTCCTCAGCCTTCTTTTCCAGTTATG
GAGGTCGACCTCCAGAAAACAGCTGCTCTTGCCCTTGCCCCCCGGCAGCCTTGGCCTCCC
TTTCATTGGCGAAACCCTCCACTTCCTCAACACGCAGAGGTGCAACACGTCCTGGAAATT
CATCGATGATCGTGTCTCCACTTATGGCGAAGTCTTCAAGACCCATCTCATCGGCGATCC
TACCATTTGTGCTTCCAATCCTTCAGGTAACAAGATGATCTTCACTGGAGAGAACAAGCT
GTGCCATGTTTCTTGGCCTGGTAGTGTAGTTACTGTGATTGGGAAGAGGTCCCTCATAGC
CTTAAACGGCGATGCGGCCAAGCGCATTCGGAGCGCTATGATGACGTTCTTGCGGCCAGA
AGCTCTTCAGGCATATACTGGAAAGACAGATAGGAGCGTACGAGGCTTTCTTGAAGAGCA
TTGGCAAGGGAAGTCTGAAGTGAAGGTGTTTCCTCTTA
■■Homology search results ■■ -
sp_hit_id Q50EK0
Definition sp|Q50EK0|C16B2_PICSI Cytochrome P450 716B2 OS=Picea sitchensis
Align length 131
Score (bit) 125.0
E-value 2.0e-28
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953261|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_E23, 5'
         (578 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q50EK0|C16B2_PICSI Cytochrome P450 716B2 OS=Picea sitchensis ...   125   2e-28
sp|Q50EK1|C16B1_PICSI Cytochrome P450 716B1 OS=Picea sitchensis ...   125   2e-28
sp|Q8W4T9|T13H_TAXCU Taxane 13-alpha-hydroxylase OS=Taxus cuspid...   114   4e-25
sp|Q9AXM6|T10H_TAXCU Taxane 10-beta-hydroxylase OS=Taxus cuspida...   112   1e-24
sp|Q42569|C90A1_ARATH Cytochrome P450 90A1 OS=Arabidopsis thalia...    85   3e-16
sp|P79739|CP26A_DANRE Cytochrome P450 26A1 OS=Danio rerio GN=cyp...    78   4e-14
sp|Q6F4F5|C724B_ORYSJ Cytochrome P450 724B1 OS=Oryza sativa subs...    76   1e-13
sp|O64989|C90B1_ARATH Cytochrome P450 90B1 OS=Arabidopsis thalia...    75   2e-13
sp|O93323|CP26A_XENLA Cytochrome P450 26A1 OS=Xenopus laevis GN=...    70   1e-11
sp|Q9M066|C90C1_ARATH Cytochrome P450 90C1 OS=Arabidopsis thalia...    69   2e-11
sp|O55127|CP26A_MOUSE Cytochrome P450 26A1 OS=Mus musculus GN=Cy...    68   4e-11
sp|O43174|CP26A_HUMAN Cytochrome P450 26A1 OS=Homo sapiens GN=CY...    68   4e-11
sp|Q59990|CP120_SYNY3 Putative cytochrome P450 120 OS=Synechocys...    67   8e-11
sp|Q9PUB4|CP26A_CHICK Cytochrome P450 26A1 OS=Gallus gallus GN=C...    65   2e-10
sp|Q6V0L0|CP26C_HUMAN Cytochrome P450 26C1 OS=Homo sapiens GN=CY...    65   3e-10
sp|Q6EIG3|CP26B_DANRE Cytochrome P450 26B1 OS=Danio rerio GN=cyp...    63   9e-10
sp|Q50EK6|C72B1_PINTA Cytochrome P450 720B1 OS=Pinus taeda GN=CY...    63   1e-09
sp|Q7XU38|C87A3_ORYSJ Cytochrome P450 87A3 OS=Oryza sativa subsp...    62   2e-09
sp|Q43147|C85A1_SOLLC Cytochrome P450 85A1 OS=Solanum lycopersic...    61   4e-09
sp|Q69F95|C85A_PHAVU Cytochrome P450 85A OS=Phaseolus vulgaris G...    60   6e-09
sp|Q0J185|ABAH3_ORYSJ Abscisic acid 8'-hydroxylase 3 OS=Oryza sa...    60   6e-09
sp|A2Z212|ABAH3_ORYSI Abscisic acid 8'-hydroxylase 3 OS=Oryza sa...    60   6e-09
sp|Q9C5Y2|KAO2_ARATH Ent-kaurenoic acid oxidase 2 OS=Arabidopsis...    59   2e-08
sp|Q6ZDE3|ABAH2_ORYSJ Abscisic acid 8'-hydroxylase 2 OS=Oryza sa...    58   3e-08
sp|Q09J78|ABAH2_ORYSI Abscisic acid 8'-hydroxylase 2 OS=Oryza sa...    58   3e-08
sp|Q50LE0|C85A3_SOLLC Cytochrome P450 85A3 OS=Solanum lycopersic...    58   4e-08
sp|Q9NR63|CP26B_HUMAN Cytochrome P450 26B1 OS=Homo sapiens GN=CY...    57   7e-08
sp|Q50EK5|C72B2_PINTA Cytochrome P450 720B2 OS=Pinus taeda GN=CY...    57   9e-08
sp|Q8GSQ1|C85A1_ORYSJ Cytochrome P450 85A1 OS=Oryza sativa subsp...    55   2e-07
sp|Q949P1|ABAH1_ARATH Abscisic acid 8'-hydroxylase 1 OS=Arabidop...    55   2e-07

>sp|Q50EK0|C16B2_PICSI Cytochrome P450 716B2 OS=Picea sitchensis
           GN=CYP716B2 PE=2 SV=1
          Length = 497

 Score =  125 bits (313), Expect = 2e-28
 Identities = 64/131 (48%), Positives = 78/131 (59%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGETL FL  QR      F D R   YG VF T L+G PT+   +P GN+ +F+ ENKL 
Sbjct: 61  IGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLV 120

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
             SWP SV  +  + SLI   GD AKR+R  +MTFLRPEAL+ + G+ D   +  L EHW
Sbjct: 121 VNSWPSSVGNLF-RSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHW 179

Query: 545 QGKSEVKVFPL 577
            GK EV   PL
Sbjct: 180 IGKDEVTALPL 190


>sp|Q50EK1|C16B1_PICSI Cytochrome P450 716B1 OS=Picea sitchensis
           GN=CYP716B1 PE=2 SV=1
          Length = 493

 Score =  125 bits (313), Expect = 2e-28
 Identities = 64/131 (48%), Positives = 78/131 (59%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGETL FL  QR      F D R   YG VF T L+G PT+   +P GN+ +F+ ENKL 
Sbjct: 61  IGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLV 120

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
             SWP SV  +  + SLI   GD AKR+R  +MTFLRPEAL+ + G+ D   +  L EHW
Sbjct: 121 VNSWPSSVGNLF-RSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHW 179

Query: 545 QGKSEVKVFPL 577
            GK EV   PL
Sbjct: 180 IGKDEVMALPL 190


>sp|Q8W4T9|T13H_TAXCU Taxane 13-alpha-hydroxylase OS=Taxus cuspidata
           GN=CYP725A2 PE=1 SV=1
          Length = 485

 Score =  114 bits (285), Expect = 4e-25
 Identities = 51/131 (38%), Positives = 84/131 (64%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           +GETL F+ +   +T  +FI++R+S +G+VFKT +IG PT+    P+GN+++ + ENKL 
Sbjct: 48  VGETLQFVRSLGSSTPQQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLV 107

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +SWP S++ +IG+  L    G+  + +R+A+  FL P+ALQ +  K    ++  + E W
Sbjct: 108 QMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQRHINEKW 167

Query: 545 QGKSEVKVFPL 577
           +GK E  V PL
Sbjct: 168 KGKDEATVLPL 178


>sp|Q9AXM6|T10H_TAXCU Taxane 10-beta-hydroxylase OS=Taxus cuspidata
           GN=CYP725A1 PE=1 SV=1
          Length = 497

 Score =  112 bits (280), Expect = 1e-24
 Identities = 54/131 (41%), Positives = 79/131 (60%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGET+  L T R  T  KF DDR+  +G V+ T LIG PT+    P+GNK++ + E+KL 
Sbjct: 60  IGETIQLLRTLRSETPQKFFDDRLKKFGPVYMTSLIGHPTVVLCGPAGNKLVLSNEDKLV 119

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +  P S + +IG+ S++A  G+  + +R+A+  FL  +ALQ Y G+    +     E W
Sbjct: 120 EMEGPKSFMKLIGEDSIVAKRGEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKW 179

Query: 545 QGKSEVKVFPL 577
           +GK EVKV PL
Sbjct: 180 KGKDEVKVLPL 190


>sp|Q42569|C90A1_ARATH Cytochrome P450 90A1 OS=Arabidopsis thaliana
           GN=CYP90A1 PE=2 SV=1
          Length = 472

 Score = 84.7 bits (208), Expect = 3e-16
 Identities = 44/124 (35%), Positives = 67/124 (54%), Gaps = 1/124 (0%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGET   +   +      FID+RV+ YG VF THL G+PTI +++P  N+ +   E KL 
Sbjct: 41  IGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLF 100

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTG-KTDRSVRGFLEEH 541
             S+P S+  ++GK SL+ + G   KR+ S  M+F     ++ +     DR VR F  + 
Sbjct: 101 ECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVR-FNLDS 159

Query: 542 WQGK 553
           W  +
Sbjct: 160 WSSR 163


>sp|P79739|CP26A_DANRE Cytochrome P450 26A1 OS=Danio rerio
           GN=cyp26a1 PE=2 SV=1
          Length = 492

 Score = 77.8 bits (190), Expect = 4e-14
 Identities = 45/131 (34%), Positives = 69/131 (52%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           FIGETL  +  +R     KF+  +   YG ++KTHL G+PT+        + I  GE+KL
Sbjct: 53  FIGETLQLILQRR-----KFLRMKRQKYGCIYKTHLFGNPTVRVMGADNVRQILLGEHKL 107

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
             V WP SV T++G  +L  ++G   K  + A+M     +AL+ Y     + V+  ++E 
Sbjct: 108 VSVQWPASVRTILGSDTLSNVHGVQHKNKKKAIMRAFSRDALEHYIPVIQQEVKSAIQEW 167

Query: 542 WQGKSEVKVFP 574
            Q  S V V+P
Sbjct: 168 LQKDSCVLVYP 178


>sp|Q6F4F5|C724B_ORYSJ Cytochrome P450 724B1 OS=Oryza sativa subsp.
           japonica GN=CYP724B1 PE=1 SV=1
          Length = 480

 Score = 76.3 bits (186), Expect = 1e-13
 Identities = 42/128 (32%), Positives = 69/128 (53%), Gaps = 1/128 (0%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           +GETL FL+    NT   F++D  S YG VFK+HL   PTI + +   N  I   E +L 
Sbjct: 45  LGETLRFLSPHASNTLGSFLEDHCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNEERLF 104

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQ-AYTGKTDRSVRGFLEEH 541
             S+P  +  ++GK S++ + G+  KR+R+  +  +    L+ +Y G  ++ +   +   
Sbjct: 105 QCSYPRPIHGILGKSSMLVVLGEDHKRLRNLALALVTSTKLKPSYLGDIEK-IALHIVGS 163

Query: 542 WQGKSEVK 565
           W GKS+ K
Sbjct: 164 WHGKSKDK 171


>sp|O64989|C90B1_ARATH Cytochrome P450 90B1 OS=Arabidopsis thaliana
           GN=CYP90B1 PE=1 SV=1
          Length = 513

 Score = 75.1 bits (183), Expect = 2e-13
 Identities = 39/125 (31%), Positives = 67/125 (53%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           F+GET+ +L      T   F+   VS YG++++++L G+PTI +++   N+ I   E +L
Sbjct: 48  FLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRL 107

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
              S+P S+  ++GK S++ L GD  + +RS  + FL    L+    K       F+ + 
Sbjct: 108 FECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDS 167

Query: 542 WQGKS 556
           WQ  S
Sbjct: 168 WQQNS 172


>sp|O93323|CP26A_XENLA Cytochrome P450 26A1 OS=Xenopus laevis
           GN=cyp26a1 PE=2 SV=1
          Length = 492

 Score = 69.7 bits (169), Expect = 1e-11
 Identities = 42/131 (32%), Positives = 63/131 (48%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           F GETL  +  +R     +F+  + S YG ++KTHL G PT+  +     + I  GE+KL
Sbjct: 53  FFGETLQMVLQRR-----RFLQVKRSQYGRIYKTHLFGSPTVRVTGAENVRQILMGEHKL 107

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
             V WP SV T++G   L  L+ +  K  +  +      EAL  Y  + +  VR  +   
Sbjct: 108 VSVHWPASVRTILGAGCLSNLHDNEHKYTKKVIAQAFSREALANYVPQMEEEVRCSVNLW 167

Query: 542 WQGKSEVKVFP 574
            Q    V V+P
Sbjct: 168 LQSGPCVLVYP 178


>sp|Q9M066|C90C1_ARATH Cytochrome P450 90C1 OS=Arabidopsis thaliana
           GN=ROT3 PE=2 SV=3
          Length = 524

 Score = 68.9 bits (167), Expect = 2e-11
 Identities = 34/102 (33%), Positives = 60/102 (58%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGETL+F+     +    F+D R S YG+VFKT++IG P I +++   NK++        
Sbjct: 79  IGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNHGNTF 138

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQ 490
             ++P S+  ++G+ S++++NG   KR+ + +  FLR   L+
Sbjct: 139 VPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLK 180
tr_hit_id Q84KI1
Definition tr|Q84KI1|Q84KI1_TAXCU Taxane 14b-hydroxylase OS=Taxus cuspidata
Align length 132
Score (bit) 118.0
E-value 2.0e-25
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953261|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_E23, 5'
         (578 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q84KI1|Q84KI1_TAXCU Taxane 14b-hydroxylase OS=Taxus cuspidata...   118   2e-25
tr|B8LM27|B8LM27_PICSI Putative uncharacterized protein OS=Picea...   118   3e-25
tr|Q56GD5|Q56GD5_TAXCH 13-alpha-hydroxylase OS=Taxus chinensis P...   117   5e-25
tr|Q6WG30|Q6WG30_TAXCU Taxadiene 5-alpha hydroxylase OS=Taxus cu...   115   2e-24
tr|Q5XQ40|Q5XQ40_TAXCH Taxadiene 5-alpha hydroxylase OS=Taxus ch...   115   2e-24
tr|Q5BU48|Q5BU48_9CONI Taxane 13-alpha-hydroxylase OS=Taxus x me...   114   3e-24
tr|Q6JTJ0|Q6JTJ0_TAXCU Taxoid 7-beta-hydroxylase OS=Taxus cuspid...   113   9e-24
tr|Q6JLD0|Q6JLD0_TAXCH Hydroxylase OS=Taxus chinensis PE=2 SV=1       113   9e-24
tr|Q6SPR0|Q6SPR0_9CONI 5-alpha-taxadienol-10-beta-hydroxylase OS...   112   1e-23
tr|Q5MCM6|Q5MCM6_9ASCO Taxane 10-beta-hydroxylase (P450 taxane 1...   112   1e-23
tr|A9S8G2|A9S8G2_PHYPA Predicted protein OS=Physcomitrella paten...   110   4e-23
tr|B8LLP7|B8LLP7_PICSI Putative uncharacterized protein OS=Picea...   109   1e-22
tr|Q6GZI3|Q6GZI3_TAXCU Taxoid 10-beta hydroxylase OS=Taxus cuspi...   108   2e-22
tr|Q8H6A8|Q8H6A8_TAXCH 5-alpha-taxadienol-10-beta-hydroxylase OS...   106   1e-21
tr|A5AVH5|A5AVH5_VITVI Putative uncharacterized protein OS=Vitis...   105   2e-21
tr|A7PFF5|A7PFF5_VITVI Chromosome chr11 scaffold_14, whole genom...   103   5e-21
tr|Q0D6C9|Q0D6C9_ORYSJ Os07g0491800 protein OS=Oryza sativa subs...   103   9e-21
tr|B8B684|B8B684_ORYSI Putative uncharacterized protein OS=Oryza...   103   9e-21
tr|A3C123|A3C123_ORYSJ Putative uncharacterized protein OS=Oryza...   103   9e-21
tr|A7P0T0|A7P0T0_VITVI Chromosome chr19 scaffold_4, whole genome...   102   1e-20
tr|A7R0M4|A7R0M4_VITVI Chromosome chr10 scaffold_312, whole geno...   102   2e-20
tr|A5BFI4|A5BFI4_VITVI Putative uncharacterized protein OS=Vitis...   102   2e-20
tr|Q2HYU7|Q2HYU7_ARTAN Putative taxane 13-alpha-hydroxylase cyto...   100   6e-20
tr|A7Q7B4|A7Q7B4_VITVI Chromosome chr18 scaffold_59, whole genom...   100   8e-20
tr|B8B6N9|B8B6N9_ORYSI Putative uncharacterized protein OS=Oryza...    99   2e-19
tr|A7PFF0|A7PFF0_VITVI Chromosome chr11 scaffold_14, whole genom...    99   2e-19
tr|A7PFB6|A7PFB6_VITVI Chromosome chr11 scaffold_14, whole genom...    99   2e-19
tr|A7P0S7|A7P0S7_VITVI Chromosome chr19 scaffold_4, whole genome...    98   4e-19
tr|Q7XIL3|Q7XIL3_ORYSJ Os07g0520300 protein OS=Oryza sativa subs...    97   5e-19
tr|A3BKE9|A3BKE9_ORYSJ Putative uncharacterized protein OS=Oryza...    97   5e-19

>tr|Q84KI1|Q84KI1_TAXCU Taxane 14b-hydroxylase OS=Taxus cuspidata
           PE=2 SV=1
          Length = 509

 Score =  118 bits (296), Expect = 2e-25
 Identities = 56/132 (42%), Positives = 81/132 (61%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           FIGE+L FL   R NT  +F+D+RV  +G VFKT LIG PT+    P+GN++I   E KL
Sbjct: 59  FIGESLLFLKALRSNTVEQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILANEEKL 118

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
             +SWP S + ++G++S+ A  G+    IRSA+  F  P ALQ Y G+  +++   + E 
Sbjct: 119 VQMSWPKSSMKLMGEKSITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEK 178

Query: 542 WQGKSEVKVFPL 577
           W+G  +V V  L
Sbjct: 179 WKGNDQVSVVAL 190


>tr|B8LM27|B8LM27_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 490

 Score =  118 bits (295), Expect = 3e-25
 Identities = 61/131 (46%), Positives = 77/131 (58%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGET+ FL  Q+  +  +F D R   YG VF T L+G PT+    P GN+ +F  ENKL 
Sbjct: 59  IGETVEFLRCQKRGSPHQFFDSRTQRYGNVFTTFLLGHPTVVFCGPEGNRFLFANENKLV 118

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
             S P S+  + G  SL+    D AKR+R  +MTFLRPEALQ + G+ D   +  L EHW
Sbjct: 119 VNSCPTSLAKLFGS-SLLTGTPDDAKRLRRMLMTFLRPEALQKFVGRVDSMTKHHLAEHW 177

Query: 545 QGKSEVKVFPL 577
            GK EV V PL
Sbjct: 178 IGKDEVTVLPL 188


>tr|Q56GD5|Q56GD5_TAXCH 13-alpha-hydroxylase OS=Taxus chinensis PE=3
           SV=1
          Length = 485

 Score =  117 bits (293), Expect = 5e-25
 Identities = 53/131 (40%), Positives = 85/131 (64%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           +GETL F+ T   +T  +FI++R+S +G+VFKT +IG PT+    P+GN+++ + ENKL 
Sbjct: 48  VGETLQFVRTLGSSTPQQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLV 107

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +SWP S++ +IG+  L    G+  + +R+A+  FL P+ALQ +  K    ++  + E W
Sbjct: 108 QMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQRHINEKW 167

Query: 545 QGKSEVKVFPL 577
           +GK EV V PL
Sbjct: 168 RGKDEVTVLPL 178


>tr|Q6WG30|Q6WG30_TAXCU Taxadiene 5-alpha hydroxylase OS=Taxus
           cuspidata PE=2 SV=2
          Length = 499

 Score =  115 bits (287), Expect = 2e-24
 Identities = 55/132 (41%), Positives = 80/132 (60%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           FIGE+  FL   R N+  +F D+RV  +G VFKT LIG PT+    P+GN++I + E KL
Sbjct: 61  FIGESFIFLRALRSNSLEQFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKL 120

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
             +SWP   + ++G+ S+    G+    +RSA+  F  P ALQ+Y GK +  ++  + E 
Sbjct: 121 VQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEK 180

Query: 542 WQGKSEVKVFPL 577
           W+GK EV V PL
Sbjct: 181 WKGKDEVNVLPL 192


>tr|Q5XQ40|Q5XQ40_TAXCH Taxadiene 5-alpha hydroxylase OS=Taxus
           chinensis PE=2 SV=1
          Length = 502

 Score =  115 bits (287), Expect = 2e-24
 Identities = 55/132 (41%), Positives = 80/132 (60%)
 Frame = +2

Query: 182 FIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKL 361
           FIGE+  FL   R N+  +F D+RV  +G VFKT LIG PT+    P+GN++I + E KL
Sbjct: 61  FIGESFIFLRALRSNSLEQFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKL 120

Query: 362 CHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEH 541
             +SWP   + ++G+ S+    G+    +RSA+  F  P ALQ+Y GK +  ++  + E 
Sbjct: 121 VQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFGPGALQSYIGKMNTEIQNHINEK 180

Query: 542 WQGKSEVKVFPL 577
           W+GK EV V PL
Sbjct: 181 WKGKDEVNVLPL 192


>tr|Q5BU48|Q5BU48_9CONI Taxane 13-alpha-hydroxylase OS=Taxus x media
           PE=2 SV=1
          Length = 485

 Score =  114 bits (286), Expect = 3e-24
 Identities = 51/131 (38%), Positives = 84/131 (64%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           +GETL F+ +   +T  +FI++R+S +G+VFKT +IG PT+    P+GN+++ + ENKL 
Sbjct: 48  VGETLQFVRSLGSSTPQQFIEERMSKFGDVFKTSIIGHPTVALCGPAGNRLVLSNENKLV 107

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +SWP S++ +IG+  L    G+  + +R+A+  FL P+ALQ +  K    ++  + E W
Sbjct: 108 QMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQRHINEKW 167

Query: 545 QGKSEVKVFPL 577
           +GK E  V PL
Sbjct: 168 KGKDEATVLPL 178


>tr|Q6JTJ0|Q6JTJ0_TAXCU Taxoid 7-beta-hydroxylase OS=Taxus cuspidata
           PE=2 SV=1
          Length = 500

 Score =  113 bits (282), Expect = 9e-24
 Identities = 54/150 (36%), Positives = 81/150 (54%)
 Frame = +2

Query: 128 TSRKQXXXXXXXXXXXXXFIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTI 307
           TS+++             FIGETL F+   R +T  +F+++R   +G VFKT L+G PT+
Sbjct: 44  TSKRRSSLKLPPGKLGLPFIGETLEFVKALRSDTLRQFVEEREGKFGRVFKTSLLGKPTV 103

Query: 308 CASNPSGNKMIFTGENKLCHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEAL 487
               P+GN+++ + E KL HVSW   +  ++G  S+    GD  + +R A+  FL    L
Sbjct: 104 ILCGPAGNRLVLSNEEKLLHVSWSAQIARILGLNSVAVKRGDDHRVLRVALAGFLGSAGL 163

Query: 488 QAYTGKTDRSVRGFLEEHWQGKSEVKVFPL 577
           Q Y GK    +R  + E W+GK EV V  L
Sbjct: 164 QLYIGKMSALIRNHINEKWKGKDEVNVLSL 193


>tr|Q6JLD0|Q6JLD0_TAXCH Hydroxylase OS=Taxus chinensis PE=2 SV=1
          Length = 493

 Score =  113 bits (282), Expect = 9e-24
 Identities = 54/150 (36%), Positives = 81/150 (54%)
 Frame = +2

Query: 128 TSRKQXXXXXXXXXXXXXFIGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTI 307
           TS+++             FIGETL F+   R +T  +F+++R   +G VFKT L+G PT+
Sbjct: 44  TSKRRSSLKLPPGKLGLPFIGETLEFVKALRSDTLRQFVEEREGKFGRVFKTSLLGKPTV 103

Query: 308 CASNPSGNKMIFTGENKLCHVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEAL 487
               P+GN+++ + E KL HVSW   +  ++G  S+    GD  + +R A+  FL    L
Sbjct: 104 ILCGPAGNRLVLSNEEKLLHVSWSAQIARILGLNSVAVKRGDDHRVLRVALAGFLGSAGL 163

Query: 488 QAYTGKTDRSVRGFLEEHWQGKSEVKVFPL 577
           Q Y GK    +R  + E W+GK EV V  L
Sbjct: 164 QLYIGKMSALIRNHINEKWKGKDEVNVLSL 193


>tr|Q6SPR0|Q6SPR0_9CONI 5-alpha-taxadienol-10-beta-hydroxylase
           OS=Taxus x media GN=10bh PE=2 SV=1
          Length = 497

 Score =  112 bits (280), Expect = 1e-23
 Identities = 54/131 (41%), Positives = 79/131 (60%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGET+  L T R  T  KF DDR+  +G V+ T LIG PT+    P+GNK++ + E+KL 
Sbjct: 60  IGETIQLLRTLRSETPQKFFDDRLKKFGPVYMTSLIGHPTVVLCGPAGNKLVLSNEDKLV 119

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +  P S + +IG+ S++A  G+  + +R+A+  FL  +ALQ Y G+    +     E W
Sbjct: 120 EMEGPKSFMKLIGEDSIVAKRGEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKW 179

Query: 545 QGKSEVKVFPL 577
           +GK EVKV PL
Sbjct: 180 KGKDEVKVLPL 190


>tr|Q5MCM6|Q5MCM6_9ASCO Taxane 10-beta-hydroxylase (P450 taxane
           10-beta-hydroxylase) OS=Ozonium sp. BT2 GN=BTPTH1 PE=2
           SV=1
          Length = 497

 Score =  112 bits (280), Expect = 1e-23
 Identities = 54/131 (41%), Positives = 79/131 (60%)
 Frame = +2

Query: 185 IGETLHFLNTQRCNTSWKFIDDRVSTYGEVFKTHLIGDPTICASNPSGNKMIFTGENKLC 364
           IGET+  L T R  T  KF DDR+  +G V+ T LIG PT+    P+GNK++ + E+KL 
Sbjct: 60  IGETIQLLRTLRSETPQKFFDDRLKKFGPVYMTSLIGHPTVVLCGPAGNKLVLSNEDKLV 119

Query: 365 HVSWPGSVVTVIGKRSLIALNGDAAKRIRSAMMTFLRPEALQAYTGKTDRSVRGFLEEHW 544
            +  P S + +IG+ S++A  G+  + +R+A+  FL  +ALQ Y G+    +     E W
Sbjct: 120 EMEGPKSFMKLIGEDSIVAKRGEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKW 179

Query: 545 QGKSEVKVFPL 577
           +GK EVKV PL
Sbjct: 180 KGKDEVKVLPL 190