NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | ダウンロード | 利用許諾 | ヘルプ
AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK953444
Clone id TST39A01NGRL0017_M14
Library
Length 580
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_M14. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
CGAGGATGAAGGCGGGCGCCGTTCTACAAAGCAAGGCGCCATGAATGCTCGGGGCTTGTT
GCGCAGTTCAGGACGAGCCCTGTAGCCCTACTGTGCAAGGCATCGAATCATCGATTCGTT
TTTTGCTGTGCATCCCGAGGATGAAGGCGGCCTTGATTAACGATGATGCAGAAGGGCTGC
GATGTCTGCTTGCTGGGGATGCTCTGTGCGCATTGAATGCCTCATGGGAGAGTCCTTTTC
AACGCGTACAGCTTTTTGCGCCTACTGTGTTGCTCACAACCTACTGAGAACTTGCCAATT
GGTGCTGCGAACAGTAGTGTGTGCTTGTATAAGAGGAGGTGGTCGCCTCTTTCTCTCTCC
ATCTAAGTCTGCCTCTTTGTCTCCATTTGAATGCGTGAGAGCATCTTGAGTGTCACTGTT
GATGGCTTGCAACCGGGACAATTGTTGTTCGTGTGCTGGCAAAGCGGAAGAATTGTGCAT
CAGCAATGGTGATTCTCTCGGAGCTGAGAGCATGGACTTCGAAGGGGGTGAGCTCCATTG
AAAGTTTTCTTTTATTTTGATGCTTTGTAAACCCTATTCT
■■Homology search results ■■ -
sp_hit_id Q8CIN9
Definition sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus norvegicus
Align length 65
Score (bit) 31.6
E-value 3.0
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953444|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_M14, 5'
         (580 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Ratt...    32   3.0  
sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Maca...    28   3.2  
sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus...    31   5.1  
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no...    30   6.7  
sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase OS...    30   8.7  
sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680 OS=A...    30   8.7  

>sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus
           norvegicus GN=Rffl PE=1 SV=1
          Length = 362

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 19/65 (29%), Positives = 33/65 (50%)
 Frame = -2

Query: 528 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 349
           P  ++ L+ P+ S +    S  LP+   Q++ + A + +TQ A+ H + D E  +  E  
Sbjct: 156 PEQQAFLTQPQSSTV-PPTSPGLPSSPAQVTSVLAQDQETQQAIGHVSQDHEEPIFLEST 214

Query: 348 RRPPP 334
            R PP
Sbjct: 215 ARVPP 219


>sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Macaca
           fascicularis GN=ZNF541 PE=2 SV=2
          Length = 804

 Score = 28.5 bits (62), Expect(2) = 3.2
 Identities = 21/82 (25%), Positives = 40/82 (48%), Gaps = 3/82 (3%)
 Frame = -2

Query: 570 LQSIKIKENFQWSSPPSKSMLSAPRESPLLM-HNSSALPA--HEQQLSRLQAINSDTQDA 400
           L+ +   E  + S P +K  L +   +PL++ H+   +P   H   ++  Q  + + +D+
Sbjct: 331 LRQVLRPEGDRHSPPGAKKPLDSTAAAPLVVPHSIPVVPVTRHVGSMAMEQEKDGEERDS 390

Query: 399 LTHSNGDKEADLDGERKRRPPP 334
                  KE+    +RK+RPPP
Sbjct: 391 -------KESSQQRKRKKRPPP 405



 Score = 20.8 bits (42), Expect(2) = 3.2
 Identities = 10/34 (29%), Positives = 14/34 (41%)
 Frame = -3

Query: 281 GCEQHSRRKKLYALKRTLP*GIQCAQSIPSKQTS 180
           GC Q   R  ++ + R L    QC+   P    S
Sbjct: 424 GCHQSRLRSPMFLVDRLLKGLFQCSPYTPPPMLS 457


>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
           musculus GN=Rasa2 PE=1 SV=1
          Length = 848

 Score = 30.8 bits (68), Expect = 5.1
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -3

Query: 461 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 366
           CQ T+ + PGCKP T  +    +I ++  R+T
Sbjct: 727 CQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
           norvegicus GN=Rasa2 PE=2 SV=1
          Length = 847

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 461 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 366
           CQ T+   PGCKP T  +    +I ++  R+T
Sbjct: 726 CQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase
           OS=Desulfotalea psychrophila GN=pnp PE=3 SV=1
          Length = 692

 Score = 30.0 bits (66), Expect = 8.7
 Identities = 21/62 (33%), Positives = 30/62 (48%)
 Frame = +2

Query: 338 GGRLFLSPSKSASLSPFECVRAS*VSLLMACNRDNCCSCAGKAEELCISNGDSLGAESMD 517
           GGR+     K      F  + +S + L++AC R+      GKA+EL  S  + LGA    
Sbjct: 150 GGRIAYIDGKYVLNPTFAELESSSMDLMVACTRNAIVMVEGKADEL--SEDEILGAIFFA 207

Query: 518 FE 523
           FE
Sbjct: 208 FE 209


>sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680
           OS=Arabidopsis thaliana GN=At4g11680 PE=2 SV=1
          Length = 390

 Score = 30.0 bits (66), Expect = 8.7
 Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
 Frame = -3

Query: 506 QLRE---NHHC*CTILPLCQHTNNNCPGCK 426
           +LRE   NHH  CT +    H N+ CP CK
Sbjct: 350 ELRELPCNHHFHCTCIDKWLHINSRCPLCK 379
tr_hit_id B0CPT8
Definition tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82)
Align length 75
Score (bit) 38.1
E-value 0.36
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK953444|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0017_M14, 5'
         (580 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (st...    38   0.36 
tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor (st...    37   0.60 
tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypa...    37   1.0  
tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein OS=Trich...    36   1.4  
tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein OS=Trypa...    35   2.3  
tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory trans...    35   4.0  
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t...    34   5.2  
tr|Q6GLY8|Q6GLY8_XENLA MGC84110 protein OS=Xenopus laevis GN=MGC...    34   6.8  
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish...    34   6.8  
tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein OS=Talar...    34   6.8  
tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative OS=Pe...    34   6.8  
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe...    34   6.8  
tr|Q10DW0|Q10DW0_ORYSJ Os03g0717900 protein OS=Oryza sativa subs...    33   8.8  
tr|A3AM41|A3AM41_ORYSJ Putative uncharacterized protein OS=Oryza...    33   8.8  
tr|A2XLF0|A2XLF0_ORYSI Putative uncharacterized protein OS=Oryza...    33   8.8  

>tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor
           (strain S238N-H82) GN=LACBIDRAFT_186956 PE=4 SV=1
          Length = 940

 Score = 38.1 bits (87), Expect = 0.36
 Identities = 22/75 (29%), Positives = 37/75 (49%)
 Frame = -2

Query: 441 LSRLQAINSDTQDALTHSNGDKEADLDGERKRRPPPLIQAHTTVRSTNWQVLSRL*ATQ* 262
           L RL   + DT+D  T  +G KEA+LD E  +R   L++ + T  S N  ++  L     
Sbjct: 602 LGRLNRKHGDTEDKRTWIHGGKEANLDREATKRKTFLVENNGTASSDNQGIMDSLAGLDM 661

Query: 261 AQKAVRVEKDSPMRH 217
           +  +++    SP  +
Sbjct: 662 SSPSIQAIGSSPQEY 676


>tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor
           (strain S238N-H82) GN=LACBIDRAFT_322427 PE=4 SV=1
          Length = 194

 Score = 37.4 bits (85), Expect = 0.60
 Identities = 33/102 (32%), Positives = 41/102 (40%), Gaps = 4/102 (3%)
 Frame = -3

Query: 551 KKTFNGAH---PLRSPCSQLRENHHC*C-TILPLCQHTNNNCPGCKPSTVTLKMLSRIQM 384
           K+  N AH   PLR   SQ    HH  C T +        NC GC      L  L R   
Sbjct: 99  KQLVNNAHRRNPLRHKASQAPLPHHAMCETSMNRSDRRERNCCGC----ARLLGLPR--- 151

Query: 383 ETKRQT*MERERGDHLLLYKHTLLFAAPIGKFSVGCEQHSRR 258
            + RQT +  E G H+L  +     ++P G  S G   H  R
Sbjct: 152 -SPRQTLVNPEEGSHILWRREHYAGSSPFGLTSSGHRTHFTR 192


>tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein
            OS=Trypanosoma cruzi GN=Tc00.1047053506559.250 PE=4 SV=1
          Length = 1559

 Score = 36.6 bits (83), Expect = 1.0
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = -2

Query: 528  PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINS-DTQDALTHSNGDKEADLDGER 352
            PPS S+ S PR SP L  +SS+ PA ++    LQAI++ D+ D      GD  +    E 
Sbjct: 1179 PPSSSLASPPRSSPSLFSSSSS-PATDKHRRVLQAISTVDSSDVAALEKGDDSSPQAQEE 1237

Query: 351  KRRPPP 334
                 P
Sbjct: 1238 TAEQEP 1243


>tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein
           OS=Trichomonas vaginalis G3 GN=TVAG_067550 PE=4 SV=1
          Length = 398

 Score = 36.2 bits (82), Expect = 1.4
 Identities = 24/74 (32%), Positives = 37/74 (50%)
 Frame = -2

Query: 528 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 349
           P S S  S+PR+S    H SSA P++ + L      N D  D  + S+ DK     G  K
Sbjct: 74  PSSNSTPSSPRKSSA-KHRSSARPSNAKPLISDSKDNDDESD--SESSEDKSRPKTGGGK 130

Query: 348 RRPPPLIQAHTTVR 307
           R+P  +++  T ++
Sbjct: 131 RKPKKIVKKTTVIK 144


>tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein
            OS=Trypanosoma brucei GN=Tb11.01.8390 PE=4 SV=1
          Length = 2135

 Score = 35.4 bits (80), Expect = 2.3
 Identities = 19/53 (35%), Positives = 29/53 (54%)
 Frame = -2

Query: 513  MLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGE 355
            +LS P  SP L+ N   LPA     SRL  ++    +A + ++GD E + +GE
Sbjct: 1038 ILSNPASSPQLLLNMCKLPAPAYMGSRLPLLSMPVHNARSDTSGDVEGETNGE 1090


>tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory
           transducer OS=Rhodopseudomonas palustris (strain BisA53)
           GN=RPE_1941 PE=3 SV=1
          Length = 568

 Score = 34.7 bits (78), Expect = 4.0
 Identities = 15/33 (45%), Positives = 23/33 (69%)
 Frame = -2

Query: 528 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRL 430
           PP + ++ A  E+ L +HN S LPAH Q+L++L
Sbjct: 63  PPPEYVIEAFLEATLALHNPSNLPAHRQRLAKL 95


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
           transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
           PE=2 SV=3
          Length = 398

 Score = 34.3 bits (77), Expect = 5.2
 Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
 Frame = +2

Query: 320 CACIRGGGR-LFLSPSKSASLSPFECVRAS*VSLLMACNRDN----CCS-CAGKAEELCI 481
           C C  G  R +   PS  A   P+ CV     S L +CNR N    C S C+   E  C 
Sbjct: 65  CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 482 SNGDS 496
             G S
Sbjct: 125 KQGTS 129


>tr|Q6GLY8|Q6GLY8_XENLA MGC84110 protein OS=Xenopus laevis
           GN=MGC84110 PE=2 SV=1
          Length = 434

 Score = 33.9 bits (76), Expect = 6.8
 Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 4/45 (8%)
 Frame = -2

Query: 558 KIKENFQWSSPPSKSM----LSAPRESPLLMHNSSALPAHEQQLS 436
           KI  N  +S  PS+      +S P+ES LL  NSS  P+H QQ++
Sbjct: 43  KIATNSSYSCKPSEVQSILNISPPQESELLNENSSPPPSHSQQIN 87


>tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein
           OS=Leishmania braziliensis GN=LbrM20_V2.4220 PE=4 SV=1
          Length = 1189

 Score = 33.9 bits (76), Expect = 6.8
 Identities = 19/57 (33%), Positives = 32/57 (56%)
 Frame = -2

Query: 510 LSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERKRRP 340
           L  P   PL+   SSA P  E  ++   A+++++  A T + G+ ++   GE+KRRP
Sbjct: 428 LHHPTLPPLVKAESSAAPTAESPVANRGALSAESA-AATANTGESDSSPAGEKKRRP 483


>tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein
           OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_027460 PE=4
           SV=1
          Length = 266

 Score = 33.9 bits (76), Expect = 6.8
 Identities = 24/70 (34%), Positives = 34/70 (48%), Gaps = 2/70 (2%)
 Frame = -2

Query: 558 KIKENFQWS--SPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSN 385
           K KE  + S  SPP+ S L++P  + ++ H      AHE  LSR     +  +D     N
Sbjct: 25  KAKEKMEESLDSPPTGSPLTSPIVTIVVGHERRLFAAHEDVLSRSAFFEAALKDQFLEPN 84

Query: 384 GDKEADLDGE 355
           G K+ DL  E
Sbjct: 85  G-KKVDLPDE 93