DK953889
Clone id TST39A01NGRL0018_P16
Library
Length 495
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_P16. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CTTCGTAGCTGACCATCACTTAGCTTCGCTCTTCGCTGTGCTTTGCAAGTTTGCACAGCC
AAACGTGAGCAATACAAGTAAGCTGTTTACGCGAATCACACTCACCTGAACAACAAGTGG
TTAGTGCTGTGGAGTTCTCCTTCACCGTTTAGCTATGGCGAGTACCATGCAGAAGCTTCC
TGCATCCGCCTCCTCTTCTTACCACCACCGCGATGCCATCCTACCCTCTCGCAGCAGCCC
GTCATTGTCTTTGAGGATGCAGTTCGTTAAGGGTGAGGTGTTTGGGTACTCCCTCTCCCT
CCCGAAGGCGCTTGTCCCTGTCAGTCCCTTGAAATCTTTGAATGGCCCCATCCCTACCTG
CAACGTTGCCACAACAACAGCTCCAGCCAAAGAGGCAGGCAAAGTTAAAGTGAGACCTGA
TATCAGGAACATTGCTATCATTGCTCATGTGGGACCATGGGAAAACGACTTTGGTGGATG
CCATGCTGAAAACAG
■■Homology search results ■■ -
sp_hit_id P72749
Definition sp|P72749|TYPA_SYNY3 GTP-binding protein TypA/BipA homolog OS=Synechocystis sp. (strain PCC 6803)
Align length 13
Score (bit) 29.3
E-value 1.2
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK953889|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0018_P16, 5'
(495 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P72749|TYPA_SYNY3 GTP-binding protein TypA/BipA homolog OS=Sy... 29 1.2
sp|Q89AC9|TYPA_BUCBP GTP-binding protein TypA/BipA homolog OS=Bu... 26 4.4
sp|Q8CNF2|GLCU_STAES Probable glucose uptake protein glcU OS=Sta... 30 6.1
sp|Q5HLZ2|GLCU_STAEQ Probable glucose uptake protein glcU OS=Sta... 30 6.1
sp|Q6PFY1|FCSD1_MOUSE FCH and double SH3 domains protein 1 OS=Mu... 30 7.9
sp|Q9X1V8|LEPA_THEMA GTP-binding protein lepA OS=Thermotoga mari... 25 9.3
sp|O25225|TYPA_HELPY GTP-binding protein TypA/BipA homolog OS=He... 26 9.3
sp|Q9ZLZ3|TYPA_HELPJ GTP-binding protein TypA/BipA homolog OS=He... 26 9.3

>sp|P72749|TYPA_SYNY3 GTP-binding protein TypA/BipA homolog
OS=Synechocystis sp. (strain PCC 6803) GN=typA PE=3 SV=1
Length = 597

Score = 29.3 bits (64), Expect(2) = 1.2
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDA+LK
Sbjct: 15 DHGKTTLVDALLK 27



Score = 22.3 bits (46), Expect(2) = 1.2
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +2

Query: 422 IRNIAIIAHV 451
IRN+AIIAHV
Sbjct: 5 IRNVAIIAHV 14


>sp|Q89AC9|TYPA_BUCBP GTP-binding protein TypA/BipA homolog
OS=Buchnera aphidicola subsp. Baizongia pistaciae
GN=typA PE=3 SV=2
Length = 611

Score = 25.8 bits (55), Expect(2) = 4.4
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVD +L+
Sbjct: 19 DHGKTTLVDKLLQ 31



Score = 23.9 bits (50), Expect(2) = 4.4
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +2

Query: 407 KVRPDIRNIAIIAHV 451
K ++RNIAIIAHV
Sbjct: 4 KTNKNLRNIAIIAHV 18


>sp|Q8CNF2|GLCU_STAES Probable glucose uptake protein glcU
OS=Staphylococcus epidermidis (strain ATCC 12228)
GN=glcU PE=3 SV=1
Length = 287

Score = 30.0 bits (66), Expect = 6.1
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = -1

Query: 432 MFLISGLTLTLPASLAGAVVVATLQVGMGPFKDFKGLT-GTSAFG 301
M ++ L LPA G+VV+ + VG GP+ +G T GT G
Sbjct: 1 MQIVDFLIALLPALFWGSVVIINVFVGGGPYNQIRGTTLGTLFIG 45


>sp|Q5HLZ2|GLCU_STAEQ Probable glucose uptake protein glcU
OS=Staphylococcus epidermidis (strain ATCC 35984 /
RP62A) GN=glcU PE=3 SV=1
Length = 287

Score = 30.0 bits (66), Expect = 6.1
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = -1

Query: 432 MFLISGLTLTLPASLAGAVVVATLQVGMGPFKDFKGLT-GTSAFG 301
M ++ L LPA G+VV+ + VG GP+ +G T GT G
Sbjct: 1 MQIVDFLIALLPALFWGSVVIINVFVGGGPYNQIRGTTLGTLFIG 45


>sp|Q6PFY1|FCSD1_MOUSE FCH and double SH3 domains protein 1 OS=Mus
musculus GN=Fchsd1 PE=2 SV=1
Length = 688

Score = 29.6 bits (65), Expect = 7.9
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +2

Query: 293 LSLPKALVPVSPLKSLNGPIPTCNVATTTAPAKEAGKV 406
LS P+ ++P S + P PTC + +TAPA + KV
Sbjct: 614 LSDPEQMLPSPSPPSFSPPAPTCALDGSTAPALPSDKV 651


>sp|Q9X1V8|LEPA_THEMA GTP-binding protein lepA OS=Thermotoga
maritima GN=lepA PE=3 SV=1
Length = 621

Score = 25.4 bits (54), Expect(2) = 9.3
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVD +L+
Sbjct: 33 DHGKTTLVDRILE 45



Score = 23.1 bits (48), Expect(2) = 9.3
Identities = 11/14 (78%), Positives = 12/14 (85%), Gaps = 1/14 (7%)
Frame = +2

Query: 413 RPD-IRNIAIIAHV 451
RPD IRNI IIAH+
Sbjct: 19 RPDLIRNICIIAHI 32


>sp|O25225|TYPA_HELPY GTP-binding protein TypA/BipA homolog
OS=Helicobacter pylori GN=typA PE=3 SV=1
Length = 599

Score = 25.8 bits (55), Expect(2) = 9.3
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3

Query: 453 DHGKTTLVDAML 488
DHGKTTLVD +L
Sbjct: 14 DHGKTTLVDGLL 25



Score = 22.7 bits (47), Expect(2) = 9.3
Identities = 9/11 (81%), Positives = 11/11 (100%)
Frame = +2

Query: 419 DIRNIAIIAHV 451
+IRNIA+IAHV
Sbjct: 3 NIRNIAVIAHV 13


>sp|Q9ZLZ3|TYPA_HELPJ GTP-binding protein TypA/BipA homolog
OS=Helicobacter pylori J99 GN=typA PE=3 SV=1
Length = 599

Score = 25.8 bits (55), Expect(2) = 9.3
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3

Query: 453 DHGKTTLVDAML 488
DHGKTTLVD +L
Sbjct: 14 DHGKTTLVDGLL 25



Score = 22.7 bits (47), Expect(2) = 9.3
Identities = 9/11 (81%), Positives = 11/11 (100%)
Frame = +2

Query: 419 DIRNIAIIAHV 451
+IRNIA+IAHV
Sbjct: 3 NIRNIAVIAHV 13


tr_hit_id A8JCK3
Definition tr|A8JCK3|A8JCK3_CHLRE GTP binding protein TypA OS=Chlamydomonas reinhardtii
Align length 35
Score (bit) 43.1
E-value 6.0e-06
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK953889|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0018_P16, 5'
(495 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A8JCK3|A8JCK3_CHLRE GTP binding protein TypA OS=Chlamydomonas... 43 6e-06
tr|Q6SYB1|Q6SYB1_TRIPR GTP-binding protein TypA OS=Trifolium pra... 41 3e-05
tr|A9PF30|A9PF30_POPTR Putative uncharacterized protein OS=Popul... 34 0.003
tr|A9YT54|A9YT54_SUASA Tyrosine phosphorylated protein A OS=Suae... 34 0.007
tr|Q00VH1|Q00VH1_OSTTA PREDICTED OJ1115_D03.25 gene product [Ory... 35 0.009
tr|Q7UQ85|Q7UQ85_RHOBA GTP-binding translation elongation factor... 34 0.041
tr|B5GDV1|B5GDV1_9ACTO GTP-binding elongation factor OS=Streptom... 30 0.11
tr|A4F8G2|A4F8G2_SACEN GTP-binding elongation factor family prot... 30 0.11
tr|B4U8A4|B4U8A4_HYDS0 GTP-binding protein TypA OS=Hydrogenobacu... 30 0.11
tr|A9REI5|A9REI5_PHYPA Predicted protein OS=Physcomitrella paten... 29 0.15
tr|A9WPA2|A9WPA2_RENSM GTP-binding protein OS=Renibacterium salm... 30 0.19
tr|A4T905|A4T905_MYCGI GTP-binding protein TypA OS=Mycobacterium... 29 0.19
tr|B0KAF5|B0KAF5_THEP3 GTP-binding protein TypA OS=Thermoanaerob... 30 0.19
tr|B0K4E9|B0K4E9_THEPX GTP-binding protein TypA OS=Thermoanaerob... 30 0.19
tr|A4X9J2|A4X9J2_SALTO GTP-binding protein TypA OS=Salinispora t... 29 0.24
tr|Q8RBE0|Q8RBE0_THETN Predicted membrane GTPase involved in str... 30 0.25
tr|B7R6I3|B7R6I3_9THEO GTP-binding protein TypA/BipA OS=Carboxyd... 30 0.25
tr|Q2J677|Q2J677_FRASC GTP-binding protein TypA OS=Frankia sp. (... 29 0.32
tr|B2V776|B2V776_SULSY GTP-binding protein TypA OS=Sulfurihydrog... 29 0.32
tr|A7B955|A7B955_9ACTO Putative uncharacterized protein OS=Actin... 28 0.41
tr|A1TDR5|A1TDR5_MYCVP GTP-binding protein TypA OS=Mycobacterium... 29 0.41
tr|A8DJE1|A8DJE1_9BACT GTP-binding protein TypA/BipA OS=Candidat... 29 0.41
tr|Q387N2|Q387N2_9TRYP Elongation factor, putative OS=Trypanosom... 28 0.52
tr|Q03QM1|Q03QM1_LACBA Stress response membrane GTPase OS=Lactob... 30 0.53
tr|Q49WN4|Q49WN4_STAS1 Putative GTPase OS=Staphylococcus saproph... 29 0.53
tr|Q2B4W6|Q2B4W6_9BACI GTP-binding protein OS=Bacillus sp. NRRL ... 29 0.53
tr|Q896E7|Q896E7_CLOTE GTP-binding protein lepA OS=Clostridium t... 30 0.53
tr|A0JYS6|A0JYS6_ARTS2 GTP-binding protein TypA OS=Arthrobacter ... 30 0.68
tr|B8HC84|B8HC84_9MICC GTP-binding protein TypA OS=Arthrobacter ... 30 0.68
tr|Q8CWS6|Q8CWS6_STRR6 GTP-binding protein TypA/BipA (Tyrosine p... 29 0.68

>tr|A8JCK3|A8JCK3_CHLRE GTP binding protein TypA OS=Chlamydomonas
reinhardtii GN=GBA1 PE=4 SV=1
Length = 683

Score = 43.1 bits (100), Expect(2) = 6e-06
Identities = 20/35 (57%), Positives = 23/35 (65%)
Frame = +2

Query: 347 PIPTCNVATTTAPAKEAGKVKVRPDIRNIAIIAHV 451
P+ C AT APA + + VR DIRNIAIIAHV
Sbjct: 61 PVTVCRTATVDAPASASSEGSVREDIRNIAIIAHV 95



Score = 30.4 bits (67), Expect(2) = 6e-06
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAMLK
Sbjct: 96 DHGKTTLVDAMLK 108


>tr|Q6SYB1|Q6SYB1_TRIPR GTP-binding protein TypA OS=Trifolium
pratense PE=2 SV=1
Length = 676

Score = 40.8 bits (94), Expect(2) = 3e-05
Identities = 25/62 (40%), Positives = 37/62 (59%), Gaps = 1/62 (1%)
Frame = +2

Query: 269 KGEVFGYSLSLPKALVPVSPLKSLNGPIPTCNVATTTAP-AKEAGKVKVRPDIRNIAIIA 445
+ ++FG SLS K + ++ + PI C+V+ T P +E K+ R DIRNIAI+A
Sbjct: 33 RNKLFGLSLSSSKPTLRIASRNNNINPIK-CSVSEVTEPITEEKRKLMRRADIRNIAIVA 91

Query: 446 HV 451
HV
Sbjct: 92 HV 93



Score = 30.4 bits (67), Expect(2) = 3e-05
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAMLK
Sbjct: 94 DHGKTTLVDAMLK 106


>tr|A9PF30|A9PF30_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 696

Score = 33.9 bits (76), Expect(2) = 0.003
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +2

Query: 335 SLNGPIPTCNVATTTAPAKEAGKVKVRPDIRNIAIIAHV 451
S + PI C+V+T + ++ ++ R DIRNIAI+AHV
Sbjct: 60 SFHSPIK-CSVSTEASTTEKRSQMMRRGDIRNIAIVAHV 97



Score = 30.4 bits (67), Expect(2) = 0.003
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAMLK
Sbjct: 98 DHGKTTLVDAMLK 110


>tr|A9YT54|A9YT54_SUASA Tyrosine phosphorylated protein A OS=Suaeda
salsa GN=TypA PE=2 SV=1
Length = 683

Score = 33.9 bits (76), Expect(2) = 0.007
Identities = 17/33 (51%), Positives = 24/33 (72%), Gaps = 2/33 (6%)
Frame = +2

Query: 359 CNVATTTA--PAKEAGKVKVRPDIRNIAIIAHV 451
C+ A T A P+++ G++ R DIRNIAI+AHV
Sbjct: 68 CSAAGTAAATPSEKKGQLMRRNDIRNIAIVAHV 100



Score = 29.3 bits (64), Expect(2) = 0.007
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVD+MLK
Sbjct: 101 DHGKTTLVDSMLK 113


>tr|Q00VH1|Q00VH1_OSTTA PREDICTED OJ1115_D03.25 gene product [Oryza
sativa (ISS) OS=Ostreococcus tauri GN=Ot15g00380 PE=4
SV=1
Length = 629

Score = 35.4 bits (80), Expect(2) = 0.009
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = +2

Query: 383 PAKEAGKVKVRPDIRNIAIIAHV 451
PA ++GK VR +IRNIAIIAHV
Sbjct: 48 PAAQSGKATVRDEIRNIAIIAHV 70



Score = 27.3 bits (59), Expect(2) = 0.009
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = +3

Query: 453 DHGKTTLVDAML 488
DHGKTTLVD+ML
Sbjct: 71 DHGKTTLVDSML 82


>tr|Q7UQ85|Q7UQ85_RHOBA GTP-binding translation elongation factor
homolog OS=Rhodopirellula baltica GN=typA PE=4 SV=1
Length = 640

Score = 33.9 bits (76), Expect(2) = 0.041
Identities = 16/39 (41%), Positives = 26/39 (66%), Gaps = 5/39 (12%)
Frame = +2

Query: 350 IPTCNVATTTAPAKEAG-----KVKVRPDIRNIAIIAHV 451
I +C +++T +P+++AG VR +IRN+ IIAHV
Sbjct: 10 IESCPLSSTASPSQDAGTDTSSNTAVRDEIRNVVIIAHV 48



Score = 26.6 bits (57), Expect(2) = 0.041
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVD +L+
Sbjct: 49 DHGKTTLVDCLLR 61


>tr|B5GDV1|B5GDV1_9ACTO GTP-binding elongation factor
OS=Streptomyces sp. SPB74 GN=SSBG_02459 PE=4 SV=1
Length = 648

Score = 30.4 bits (67), Expect(2) = 0.11
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAMLK
Sbjct: 41 DHGKTTLVDAMLK 53



Score = 28.5 bits (62), Expect(2) = 0.11
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +2

Query: 392 EAGKVKVRPDIRNIAIIAHV 451
E+ +V R DIRN+AI+AHV
Sbjct: 21 ESPQVPTRHDIRNVAIVAHV 40


>tr|A4F8G2|A4F8G2_SACEN GTP-binding elongation factor family protein
OS=Saccharopolyspora erythraea (strain NRRL 23338)
GN=typA PE=4 SV=1
Length = 641

Score = 29.6 bits (65), Expect(2) = 0.11
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +2

Query: 368 ATTTAPAKEAGKVKVRPDIRNIAIIAHV 451
AT+ AP ++ VR D+RN+AI+AHV
Sbjct: 3 ATSVAPTQQ-----VRSDLRNVAIVAHV 25



Score = 29.3 bits (64), Expect(2) = 0.11
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAML+
Sbjct: 26 DHGKTTLVDAMLR 38


>tr|B4U8A4|B4U8A4_HYDS0 GTP-binding protein TypA OS=Hydrogenobaculum
sp. (strain Y04AAS1) GN=HY04AAS1_0678 PE=4 SV=1
Length = 594

Score = 29.6 bits (65), Expect(2) = 0.11
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = +2

Query: 404 VKVRPDIRNIAIIAHV 451
+K+R DIRN+AIIAHV
Sbjct: 1 MKIREDIRNVAIIAHV 16



Score = 29.3 bits (64), Expect(2) = 0.11
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDA+LK
Sbjct: 17 DHGKTTLVDALLK 29


>tr|A9REI5|A9REI5_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_65080 PE=4 SV=1
Length = 626

Score = 29.3 bits (64), Expect(2) = 0.15
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +2

Query: 401 KVKVRPDIRNIAIIAHV 451
K VR D+RNIAI+AHV
Sbjct: 26 KTSVRTDVRNIAIVAHV 42



Score = 29.3 bits (64), Expect(2) = 0.15
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3

Query: 453 DHGKTTLVDAMLK 491
DHGKTTLVDAML+
Sbjct: 43 DHGKTTLVDAMLR 55