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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK954383
Clone id TST39A01NGRL0020_E18
Library
Length 641
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_E18. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
CCCCTCCTCCAATCCTAGCATTTAAGGGTCATTCTGATCGTCACCGCCTTGCAATATTAA
GCGTCTTTGCAGCCGAGTTCTAGATCCGCATCCGTCCTTCTGGAGCTCGCCATAAAAACT
CCAGCTTTTGATTTGTAATCCTCCTTTTAGGGCCGTGGTTCATGCGCAACAGTAGACCGT
CTTAGTGATGTTGGTGCTCTGTGAATCTTTACCATCTTGCACACGCTGTGCTAAAAAGTT
GCTGCACATACGCTGCAAGGATCCACTCTGAAGGCTCTCTAAATTATGGTGTAGAATTGC
TCGGCAGTTAGGGACTTGGAGGGCACAGAGATGGTGTGACTCGAAGGATCAAGATGTAGG
GAGAGCGAAAGCATGGTCTAGGCTGCAAGGAAAGGCAGAATTCTTTAAGAGGCAAAATTC
TGCTGCTAGTGGTGATCTTCTGCGGAATGGAACTCGGTTCATCGGCAGATCAAAGCTCGG
AATCTGAATGTGCTCATCGACAAGAGGTACTGTTTCTAAGTCTGTTCCTTGCACATTCCC
ATCGTCCACCTTCGCTCAGCATATATCCGGCTTACCCATGCCCTCCTGCCCTCAATGCTT
GCCCCCTGCTTGTAGACAACCCCGGAGGAGGGGCTTGAAGG
■■Homology search results ■■ -
sp_hit_id A4IYB1
Definition sp|A4IYB1|MIAA_FRATW tRNA delta(2)-isopentenylpyrophosphate transferase OS=Francisella tularensis subsp. tularensis (strain WY96-3418)
Align length 100
Score (bit) 32.0
E-value 2.8
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK954383|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0020_E18, 5'
         (641 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A4IYB1|MIAA_FRATW tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|Q5NH42|MIAA_FRATT tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|Q0BM87|MIAA_FRATO tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|A0Q6S4|MIAA_FRATN tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|Q2A3T6|MIAA_FRATH tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|A7NBS1|MIAA_FRATF tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|Q14IJ4|MIAA_FRAT1 tRNA delta(2)-isopentenylpyrophosphate tran...    32   2.8  
sp|Q4PBF2|NOP58_USTMA Nucleolar protein NOP58 OS=Ustilago maydis...    31   6.2  
sp|P75405|Y376_MYCPN Uncharacterized protein MPN_376 OS=Mycoplas...    30   8.1  
sp|O74755|PST3_SCHPO Paired amphipathic helix protein pst3 OS=Sc...    30   8.1  
sp|Q0RDQ5|EFTS_FRAAA Elongation factor Ts OS=Frankia alni (strai...    30   8.1  

>sp|A4IYB1|MIAA_FRATW tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. tularensis
           (strain WY96-3418) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|Q5NH42|MIAA_FRATT tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. tularensis
           GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|Q0BM87|MIAA_FRATO tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. holarctica
           (strain OSU18) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|A0Q6S4|MIAA_FRATN tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. novicida
           (strain U112) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EELKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLRKNRMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|Q2A3T6|MIAA_FRATH tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. holarctica
           (strain LVS) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|A7NBS1|MIAA_FRATF tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. holarctica
           (strain FTA) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|Q14IJ4|MIAA_FRAT1 tRNA delta(2)-isopentenylpyrophosphate
           transferase OS=Francisella tularensis subsp. tularensis
           (strain FSC 198) GN=miaA PE=3 SV=1
          Length = 308

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 6/100 (6%)
 Frame = -3

Query: 342 ESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA 163
           E   LCAL VPN R+ILH N+ES               Q +  G   +  N+ K      
Sbjct: 189 EDLKLCAL-VPNDRSILHKNIESR------------FRQMLDQGFLDEVQNLHKNPMLTK 235

Query: 162 *TTALKGGLQIKSWSF------YGELQKDGCGSRTRLQRR 61
            TTA++     ++W +      Y E  K G  +  +L +R
Sbjct: 236 ETTAIRSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKR 275


>sp|Q4PBF2|NOP58_USTMA Nucleolar protein NOP58 OS=Ustilago maydis
           GN=NOP58 PE=3 SV=1
          Length = 582

 Score = 30.8 bits (68), Expect = 6.2
 Identities = 21/87 (24%), Positives = 36/87 (41%)
 Frame = -3

Query: 285 NLESLQSGSLQRMCSNFLAQRVQDGKDSQSTNITKTVYCCA*TTALKGGLQIKSWSFYGE 106
           ++ ++  G + +    FL   + +GK  +  N+       A   A K G+Q+   ++  E
Sbjct: 60  DISAINEGKMSKSLKKFLTDEIVEGKGKEKENLIVAESKLASNIAKKLGIQV---TYDSE 116

Query: 105 LQKDGCGSRTRLQRRLILQGGDDQNDP 25
           L     G R  L   L    GD+  DP
Sbjct: 117 LLDLYRGIRENLATLLSSSSGDESLDP 143


>sp|P75405|Y376_MYCPN Uncharacterized protein MPN_376 OS=Mycoplasma
            pneumoniae GN=MPN_376 PE=4 SV=1
          Length = 1140

 Score = 30.4 bits (67), Expect = 8.1
 Identities = 32/128 (25%), Positives = 53/128 (41%), Gaps = 3/128 (2%)
 Frame = -3

Query: 402  NSAFPCSLDHA-FALPTS*SFESHHLCALQVPNCRAILHHNLESLQSGSLQRMCSN--FL 232
            N+A   +L +A   LPT+ +  S  L      N    L  N+     G ++RM     FL
Sbjct: 835  NAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFL 894

Query: 231  AQRVQDGKDSQSTNITKTVYCCA*TTALKGGLQIKSWSFYGELQKDGCGSRTRLQRRLIL 52
             +RV   K+ +  N  K       T   KG L++K  S   ++ ++  G    +      
Sbjct: 895  KERVSFNKEDK--NNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEE--- 949

Query: 51   QGGDDQND 28
            +G D ++D
Sbjct: 950  KGDDPESD 957


>sp|O74755|PST3_SCHPO Paired amphipathic helix protein pst3
           OS=Schizosaccharomyces pombe GN=pst3 PE=2 SV=1
          Length = 1154

 Score = 30.4 bits (67), Expect = 8.1
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +1

Query: 436 SSAEWNSVHRQIKARNLNVLIDKRYC 513
           S  EW+ + RQI+ +N     D RYC
Sbjct: 646 SKREWSKIWRQIEKKNAQAAFDDRYC 671
tr_hit_id Q5YEW2
Definition tr|Q5YEW2|Q5YEW2_FRAAN CTR1-like protein kinase (Fragment) OS=Fragaria ananassa
Align length 50
Score (bit) 35.4
E-value 3.0
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK954383|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0020_E18, 5'
         (641 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q5YEW2|Q5YEW2_FRAAN CTR1-like protein kinase (Fragment) OS=Fr...    35   3.0  
tr|B8LKC5|B8LKC5_PICSI Putative uncharacterized protein OS=Picea...    35   5.1  
tr|B8P7J3|B8P7J3_9APHY Predicted protein OS=Postia placenta Mad-...    35   5.1  
tr|B8P7K9|B8P7K9_9APHY Predicted protein OS=Postia placenta Mad-...    34   6.6  
tr|B8P5Q1|B8P5Q1_9APHY Predicted protein OS=Postia placenta Mad-...    34   6.6  

>tr|Q5YEW2|Q5YEW2_FRAAN CTR1-like protein kinase (Fragment)
           OS=Fragaria ananassa GN=CTR1 PE=2 SV=1
          Length = 142

 Score = 35.4 bits (80), Expect = 3.0
 Identities = 20/50 (40%), Positives = 30/50 (60%)
 Frame = +1

Query: 457 VHRQIKARNLNVLIDKRYCF*VCSLHIPIVHLRSAYIRLTHALLPSMLAP 606
           VHR +K+ NL  L+DK+Y   VC L  P+     ++I++T A  P  +AP
Sbjct: 91  VHRDLKSPNL--LVDKKYTVKVCDLVFPVKGQHLSFIKIT-AGTPEWMAP 137


>tr|B8LKC5|B8LKC5_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 424

 Score = 34.7 bits (78), Expect = 5.1
 Identities = 26/79 (32%), Positives = 36/79 (45%)
 Frame = +2

Query: 350 QDVGRAKAWSRLQGKAEFFKRQNSAASGDLLRNGTRFIGRSKLGI*MCSSTRGTVSKSVP 529
           QD  R K +  + G  EF K       GD    G+  IG  ++    C S  GT S  V 
Sbjct: 80  QDRSRYKEYIPIAGLVEFNKLSAKLILGD----GSPAIGEKRVATAQCLS--GTGSLRVG 133

Query: 530 CTFPSSTFAQHISGLPMPS 586
             F S  ++QHI  +P+P+
Sbjct: 134 AEFLSKHYSQHIIYIPVPT 152


>tr|B8P7J3|B8P7J3_9APHY Predicted protein OS=Postia placenta
           Mad-698-R GN=POSPLDRAFT_101038 PE=4 SV=1
          Length = 704

 Score = 34.7 bits (78), Expect = 5.1
 Identities = 18/54 (33%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
 Frame = -3

Query: 510 VPLVDEHIQIPSFD--LPMNRVPFRRRSPLAAEFCLLKNSAFPCSLDHAFALPT 355
           +P  ++H ++PS    LP   +  R  SP++A+F ++ +SA P  L  +F LP+
Sbjct: 593 LPRFEQHARVPSTPPILPEIHIQQRAFSPVSADFPIVPSSARPSPLSSSFGLPS 646


>tr|B8P7K9|B8P7K9_9APHY Predicted protein OS=Postia placenta
           Mad-698-R GN=POSPLDRAFT_93364 PE=4 SV=1
          Length = 711

 Score = 34.3 bits (77), Expect = 6.6
 Identities = 27/91 (29%), Positives = 37/91 (40%)
 Frame = +2

Query: 344 KDQDVGRAKAWSRLQGKAEFFKRQNSAASGDLLRNGTRFIGRSKLGI*MCSSTRGTVSKS 523
           K +   +AK  +  +GKA+    +N A +G+    G RF          C  T G  S+S
Sbjct: 573 KQKARAKAKRMTEAKGKAKASSTKNKAKAGNTKSEGERFPCLQA----GCDQTFGRQSES 628

Query: 524 VPCTFPSSTFAQHISGLPMPSCPQCLPPACR 616
               F  S   +    L  P CP C  P CR
Sbjct: 629 SR-HFRCSCPKRDPDDLEKPPCPHCKEPLCR 658


>tr|B8P5Q1|B8P5Q1_9APHY Predicted protein OS=Postia placenta
           Mad-698-R GN=POSPLDRAFT_90925 PE=4 SV=1
          Length = 800

 Score = 34.3 bits (77), Expect = 6.6
 Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 2/54 (3%)
 Frame = -3

Query: 510 VPLVDEHIQIPSFD--LPMNRVPFRRRSPLAAEFCLLKNSAFPCSLDHAFALPT 355
           +P  D+H ++PS    LP   +  R  SP++A++ +  +SA P  L  +F LP+
Sbjct: 689 LPRFDQHARVPSTPPILPEIHIQQRAFSPVSADYPIAPSSARPSPLSSSFGLPS 742