DK954504
Clone id TST39A01NGRL0020_J21
Library
Length 585
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_J21. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
AGCCGCCTTTCTCACCTGGCGACCCCCATCTCAGAGCTCATCCTGTTCGGCCTCTAGCTC
GTCATCTCCTCCTCGTTCCTCCGGGTCCGCTTGCGCTTTTGTACAAACCCTCCAATTCAG
AAATTATCCTAGTCACGCGAGCCCTTTTTCAACCTCATTGCACGTGAGCTCCTTCTGCTC
TTCCTCAAGGAGTTGCAAGCACGTACGATGCTCACCTCGCTCGATGGCGCCTGACGAAGA
GAAAATGACCAGACGATCACCTCTTGATTTTCCCATTGAATGGGAGAGACCCCGACCTGG
AAGACGGCCAGATATTTTTCCGCAGTTCAGTCCAATGAAAACTCCACTCCCTCAGCCTCC
TCCAACTGATCCTCCAGAGGAAGATGAAGAGGAAGAGAAAAAAGAAGAAGAAGAGAAGCC
AGAGGAGGAGCCTGATACACCACCTAAAGAAACATGATTCTTACTGGTCTTCTCAGTAAT
GGTGTTTTGGGTGAGCTGGGTGGTGTGCTTCAAAGAGGTGACTGATTTTTTCAAAGCTAA
CGGGGTGCAAGAATTGCCAAACCTGGTTTCTGCTCTCACACTCTT
■■Homology search results ■■ -
sp_hit_id B1J1K3
Definition sp|B1J1K3|TTCA_PSEPW tRNA 2-thiocytidine biosynthesis protein ttcA OS=Pseudomonas putida (strain W619)
Align length 35
Score (bit) 31.6
E-value 3.0
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954504|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0020_J21, 5'
(585 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|B1J1K3|TTCA_PSEPW tRNA 2-thiocytidine biosynthesis protein tt... 32 3.0
sp|Q88MD2|TTCA_PSEPK tRNA 2-thiocytidine biosynthesis protein tt... 32 3.0
sp|B0KT32|TTCA_PSEPG tRNA 2-thiocytidine biosynthesis protein tt... 32 3.0
sp|A5W7U0|TTCA_PSEP1 tRNA 2-thiocytidine biosynthesis protein tt... 32 3.0
sp|Q1IDN2|TTCA_PSEE4 tRNA 2-thiocytidine biosynthesis protein tt... 32 3.0
sp|Q3IZ05|TRMD_RHOS4 tRNA (guanine-N(1)-)-methyltransferase OS=R... 31 5.2
sp|Q48FG8|TTCA_PSE14 tRNA 2-thiocytidine biosynthesis protein tt... 30 6.7
sp|Q8Y306|TTCA_RALSO tRNA 2-thiocytidine biosynthesis protein tt... 30 8.8
sp|B2RUR4|KPRP_MOUSE Keratinocyte proline-rich protein OS=Mus mu... 30 8.8

>sp|B1J1K3|TTCA_PSEPW tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas putida (strain W619) GN=ttcA PE=3 SV=1
Length = 274

Score = 31.6 bits (70), Expect = 3.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D +EWER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLVEWERKHPGRTESIF 236


>sp|Q88MD2|TTCA_PSEPK tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas putida (strain KT2440) GN=ttcA PE=3 SV=1
Length = 274

Score = 31.6 bits (70), Expect = 3.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D +EWER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLVEWERKHPGRTESIF 236


>sp|B0KT32|TTCA_PSEPG tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas putida (strain GB-1) GN=ttcA PE=3 SV=1
Length = 274

Score = 31.6 bits (70), Expect = 3.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D +EWER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLVEWERKHPGRTESIF 236


>sp|A5W7U0|TTCA_PSEP1 tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ttcA
PE=3 SV=1
Length = 274

Score = 31.6 bits (70), Expect = 3.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D +EWER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLVEWERKHPGRTESIF 236


>sp|Q1IDN2|TTCA_PSEE4 tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas entomophila (strain L48) GN=ttcA PE=3
SV=1
Length = 274

Score = 31.6 bits (70), Expect = 3.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D +EWER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLVEWERKHPGRTESIF 236


>sp|Q3IZ05|TRMD_RHOS4 tRNA (guanine-N(1)-)-methyltransferase
OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 /
NCIB 8253 / DSM 158) GN=trmD PE=3 SV=2
Length = 249

Score = 30.8 bits (68), Expect = 5.2
Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 6/51 (11%)
Frame = +3

Query: 180 LPQGVASTYDAHLARWR------LTKRK*PDDHLLIFPLNGRDPDLEDGQI 314
+P+ + S + A++ARWR LTK + PD GRDPD ED ++
Sbjct: 200 IPEVLLSGHHANIARWRRAEAERLTKERRPDLWRAYCAARGRDPD-EDREL 249


>sp|Q48FG8|TTCA_PSE14 tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Pseudomonas syringae pv. phaseolicola (strain 1448A /
Race 6) GN=ttcA PE=3 SV=1
Length = 274

Score = 30.4 bits (67), Expect = 6.7
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ D I+WER PGR IF
Sbjct: 202 PCNLCGSQENLQRQVVKDMLIDWERKTPGRTESIF 236


>sp|Q8Y306|TTCA_RALSO tRNA 2-thiocytidine biosynthesis protein ttcA
OS=Ralstonia solanacearum GN=ttcA PE=3 SV=1
Length = 312

Score = 30.0 bits (66), Expect = 8.8
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +2

Query: 215 PRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIF 319
P ++ +E + R+ + +EWER PGR +IF
Sbjct: 201 PCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIF 235


>sp|B2RUR4|KPRP_MOUSE Keratinocyte proline-rich protein OS=Mus
musculus GN=Kprp PE=2 SV=1
Length = 657

Score = 30.0 bits (66), Expect = 8.8
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +2

Query: 209 CSPRSMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLPQ 355
C P+ + E +R P+ P + RP+P P+ P P P P+
Sbjct: 431 CRPQHLDRSPESSRQRCPVPAPRPYPRPQPCPSPEPRPYPRPQPCPSPE 479


tr_hit_id A7P7B9
Definition tr|A7P7B9|A7P7B9_VITVI Chromosome chr9 scaffold_7, whole genome shotgun sequence OS=Vitis vinifera
Align length 43
Score (bit) 94.7
E-value 3.0e-18
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954504|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0020_J21, 5'
(585 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A7P7B9|A7P7B9_VITVI Chromosome chr9 scaffold_7, whole genome ... 95 3e-18
tr|A9PBZ2|A9PBZ2_POPTR Putative uncharacterized protein OS=Popul... 94 7e-18
tr|A9RTT9|A9RTT9_PHYPA Predicted protein OS=Physcomitrella paten... 93 1e-17
tr|A9RFX0|A9RFX0_PHYPA Predicted protein OS=Physcomitrella paten... 93 1e-17
tr|Q9SII4|Q9SII4_ARATH Expressed protein (Putative uncharacteriz... 92 2e-17
tr|A7PEQ7|A7PEQ7_VITVI Chromosome chr11 scaffold_13, whole genom... 92 2e-17
tr|Q6K5Y3|Q6K5Y3_ORYSJ Os02g0614200 protein OS=Oryza sativa subs... 90 8e-17
tr|B8AFF4|B8AFF4_ORYSI Putative uncharacterized protein OS=Oryza... 90 8e-17
tr|B6UA24|B6UA24_MAIZE Putative uncharacterized protein OS=Zea m... 90 8e-17
tr|B6U119|B6U119_MAIZE Putative uncharacterized protein OS=Zea m... 90 8e-17
tr|B6TEN8|B6TEN8_MAIZE Putative uncharacterized protein OS=Zea m... 90 8e-17
tr|B6SN44|B6SN44_MAIZE Putative uncharacterized protein OS=Zea m... 90 8e-17
tr|Q9LV56|Q9LV56_ARATH Gb|AAD25142.1 (Putative uncharacterized p... 90 1e-16
tr|Q8GWM3|Q8GWM3_ARATH Putative uncharacterized protein At3g2450... 89 1e-16
tr|Q8LE64|Q8LE64_ARATH Putative uncharacterized protein OS=Arabi... 87 7e-16
tr|A3A914|A3A914_ORYSJ Putative uncharacterized protein OS=Oryza... 60 7e-08
tr|A8JCL4|A8JCL4_CHLRE Predicted protein OS=Chlamydomonas reinha... 52 2e-05
tr|A4RRA5|A4RRA5_OSTLU Predicted protein OS=Ostreococcus lucimar... 45 0.002
tr|Q6GZT3|Q6GZT3_FRG3V Putative uncharacterized protein OS=Frog ... 35 2.4

>tr|A7P7B9|A7P7B9_VITVI Chromosome chr9 scaffold_7, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00034399001
PE=4 SV=1
Length = 84

Score = 94.7 bits (234), Expect = 3e-18
Identities = 41/43 (95%), Positives = 43/43 (100%)
Frame = +2

Query: 224 MAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
MAP+EEKMTRRSPLDFPIEWERP+PGRRPDIFPQFSPMKTPLP
Sbjct: 1 MAPEEEKMTRRSPLDFPIEWERPKPGRRPDIFPQFSPMKTPLP 43


>tr|A9PBZ2|A9PBZ2_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 147

Score = 93.6 bits (231), Expect = 7e-18
Identities = 40/44 (90%), Positives = 43/44 (97%)
Frame = +2

Query: 221 SMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
SMAPDEEK+TRRSPLDFPIEWERP+PG RPDIFPQFSPMKTP+P
Sbjct: 65 SMAPDEEKLTRRSPLDFPIEWERPKPGSRPDIFPQFSPMKTPIP 108


>tr|A9RTT9|A9RTT9_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_56105 PE=4 SV=1
Length = 78

Score = 93.2 bits (230), Expect = 1e-17
Identities = 41/43 (95%), Positives = 42/43 (97%)
Frame = +2

Query: 224 MAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
MAPDEEKMTRRSPLDFPIEW+RPRPGRR DIFPQFSPMKTPLP
Sbjct: 1 MAPDEEKMTRRSPLDFPIEWDRPRPGRRADIFPQFSPMKTPLP 43


>tr|A9RFX0|A9RFX0_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_65594 PE=4 SV=1
Length = 79

Score = 93.2 bits (230), Expect = 1e-17
Identities = 41/43 (95%), Positives = 42/43 (97%)
Frame = +2

Query: 224 MAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
MAPDEEKMTRRSPLDFPIEW+RPRPGRR DIFPQFSPMKTPLP
Sbjct: 1 MAPDEEKMTRRSPLDFPIEWDRPRPGRRADIFPQFSPMKTPLP 43


>tr|Q9SII4|Q9SII4_ARATH Expressed protein (Putative uncharacterized
protein) (At2g17240/T23A1.10) OS=Arabidopsis thaliana
GN=At2g17240 PE=2 SV=1
Length = 140

Score = 92.4 bits (228), Expect = 2e-17
Identities = 39/44 (88%), Positives = 44/44 (100%)
Frame = +2

Query: 221 SMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
SMAP+EEK+TRR+PLDFPIEWERP+PGRRPDIFP+FSPMKTPLP
Sbjct: 60 SMAPEEEKLTRRNPLDFPIEWERPKPGRRPDIFPKFSPMKTPLP 103


>tr|A7PEQ7|A7PEQ7_VITVI Chromosome chr11 scaffold_13, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00016751001
PE=4 SV=1
Length = 147

Score = 92.0 bits (227), Expect = 2e-17
Identities = 39/44 (88%), Positives = 43/44 (97%)
Frame = +2

Query: 221 SMAPDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
SMAP+EEK+TRR+PLDFPIEW RP+PGRRPDIFPQFSPMKTPLP
Sbjct: 64 SMAPEEEKLTRRNPLDFPIEWNRPKPGRRPDIFPQFSPMKTPLP 107


>tr|Q6K5Y3|Q6K5Y3_ORYSJ Os02g0614200 protein OS=Oryza sativa subsp.
japonica GN=OJ2055_H10.18 PE=2 SV=1
Length = 144

Score = 90.1 bits (222), Expect = 8e-17
Identities = 38/42 (90%), Positives = 42/42 (100%)
Frame = +2

Query: 227 APDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
APDEEK+TRRSPLDFPIEWE+P+PGRRPDIFP+FSPMKTPLP
Sbjct: 57 APDEEKITRRSPLDFPIEWEKPKPGRRPDIFPKFSPMKTPLP 98


>tr|B8AFF4|B8AFF4_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_08059 PE=4 SV=1
Length = 144

Score = 90.1 bits (222), Expect = 8e-17
Identities = 38/42 (90%), Positives = 42/42 (100%)
Frame = +2

Query: 227 APDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
APDEEK+TRRSPLDFPIEWE+P+PGRRPDIFP+FSPMKTPLP
Sbjct: 57 APDEEKITRRSPLDFPIEWEKPKPGRRPDIFPKFSPMKTPLP 98


>tr|B6UA24|B6UA24_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 131

Score = 90.1 bits (222), Expect = 8e-17
Identities = 38/42 (90%), Positives = 42/42 (100%)
Frame = +2

Query: 227 APDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
APDEEK+T+RSPLDFPIEWERP+PGRRPDIFP+FSPMKTPLP
Sbjct: 44 APDEEKITKRSPLDFPIEWERPKPGRRPDIFPKFSPMKTPLP 85


>tr|B6U119|B6U119_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 140

Score = 90.1 bits (222), Expect = 8e-17
Identities = 38/42 (90%), Positives = 42/42 (100%)
Frame = +2

Query: 227 APDEEKMTRRSPLDFPIEWERPRPGRRPDIFPQFSPMKTPLP 352
APDEEK+T+RSPLDFPIEWERP+PGRRPDIFP+FSPMKTPLP
Sbjct: 53 APDEEKITKRSPLDFPIEWERPKPGRRPDIFPKFSPMKTPLP 94