DK954806
Clone id TST39A01NGRL0021_G20
Library
Length 395
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0021_G20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
TGCAAGTGTGGTAAGAAGGTGTTTGACGAAACTGCCCTCGATGCCCCGAGGGAAGTGACG
GTTGCTGGCTGTGCGTGCAAGTGTGGATCTAACTGCCAGTGCGCCGACCCCTGTCCCTGT
GCGCAAACCTCGAGCGGGGATGCTTGCAAGTGCGGTGCGAACTGTACCTGCAATCCCTGC
AACTGCTGATTATGATCATGATTAGTACTAACTTGCGCCATGTAGAAATTACCCTGTTGA
AAGATGCGATGCACGCATAGGCAAGCATGATGGTATGGCTGTCTGCTCGCCACTTGCTCC
ATCTTCATGTATCTTAGAAAGATAAATTTCACTTTTTGCTGGTATATCTTTACCATGTCT
CATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id O24528
Definition sp|O24528|MT1_FESRU Metallothionein-like protein 1 OS=Festuca rubra
Align length 57
Score (bit) 40.0
E-value 0.004
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954806|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0021_G20, 5'
(365 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|O24528|MT1_FESRU Metallothionein-like protein 1 OS=Festuca ru... 40 0.004
sp|Q43515|MT2Z_SOLLC Metallothionein-like protein type 2 OS=Sola... 36 0.052
sp|Q43512|MT2X_SOLLC Metallothionein-like protein type 2 OS=Sola... 36 0.069
sp|Q40157|MT2A_SOLLC Metallothionein-like protein type 2 A OS=So... 36 0.069
sp|P43399|MT1_TRIRP Metallothionein-like protein 1 OS=Trifolium ... 36 0.069
sp|Q41670|MT1B_VICFA Metallothionein-like protein 1B OS=Vicia fa... 36 0.069
sp|P43402|MT54_BRANA Metallothionein-like protein LSC54 OS=Brass... 35 0.089
sp|Q43513|MT2Y_SOLLC Metallothionein-like protein type 2 OS=Sola... 35 0.089
sp|P20830|MT1_PEA Metallothionein-like protein 1 OS=Pisum sativu... 35 0.12
sp|Q41669|MT1A_VICFA Metallothionein-like protein 1A OS=Vicia fa... 35 0.12
sp|P55946|MT_ARIAR Metallothionein OS=Arianta arbustorum PE=1 SV=1 35 0.15
sp|P20238|MT1_MIMGU Metallothionein-like protein 1 OS=Mimulus gu... 35 0.15
sp|Q94550|MT_DREPO Metallothionein OS=Dreissena polymorpha PE=1 ... 34 0.20
sp|P33187|MTCD_HELPO Cadmium-metallothionein OS=Helix pomatia PE... 34 0.20
sp|Q54J58|Y7870_DICDI Uncharacterized protein DDB_G0288281 OS=Di... 34 0.26
sp|Q40410|MT2_NICPL Metallothionein-like protein type 2 OS=Nicot... 34 0.26
sp|P69164|MT2_BRARA Metallothionein-like protein type 2 OS=Brass... 34 0.26
sp|P69163|MT22_BRAJU Metallothionein-like protein type 2, MT2-18... 34 0.26
sp|Q38804|MT1C_ARATH Metallothionein-like protein 1C OS=Arabidop... 34 0.26
sp|P25860|MT2A_ARATH Metallothionein-like protein 2A OS=Arabidop... 33 0.34
sp|P30571|MT1_MAIZE Metallothionein-like protein 1 OS=Zea mays P... 33 0.34
sp|O22319|MT2_MUSAC Metallothionein-like protein type 2 OS=Musa ... 33 0.44
sp|P23038|MT_CRAVI Metallothionein OS=Crassostrea virginica PE=1... 33 0.58
sp|Q40158|MT2B_SOLLC Metallothionein-like protein type 2 B OS=So... 33 0.58
sp|Q39511|MT1_CASGL Metallothionein-like protein 1 OS=Casuarina ... 32 0.76
sp|P43398|MT2_TRIRP Metallothionein-like protein 2 OS=Trifolium ... 32 0.99
sp|Q39269|MT2_BRARP Metallothionein-like protein BIF98 OS=Brassi... 32 0.99
sp|P43390|MT2_ACTDE Metallothionein-like protein type 2 OS=Actin... 32 0.99
sp|Q39458|MT1_CICAR Metallothionein-like protein 1 OS=Cicer arie... 32 0.99
sp|P37359|MT3_BOVIN Metallothionein-3 OS=Bos taurus GN=MT3 PE=1 ... 32 1.3

>sp|O24528|MT1_FESRU Metallothionein-like protein 1 OS=Festuca rubra
GN=MT1 PE=3 SV=1
Length = 70

Score = 40.0 bits (92), Expect = 0.004
Identities = 23/57 (40%), Positives = 26/57 (45%), Gaps = 17/57 (29%)
Frame = +1

Query: 1 CKCGKKV--FDETALDAPREVTVAGCA---------------CKCGSNCQCADPCPC 120
CKCGK DE A + V V G A CKCG+NC+C DPC C
Sbjct: 15 CKCGKMYPDLDEQASTTTQAVVVVGVAHENKAGQFEMASGEGCKCGANCKC-DPCNC 70


>sp|Q43515|MT2Z_SOLLC Metallothionein-like protein type 2 OS=Solanum
lycopersicum PE=3 SV=1
Length = 84

Score = 36.2 bits (82), Expect = 0.052
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1

Query: 52 EVTVAGCACKCGSNCQCADPCPCAQTSS 135
+ T G CKCGS+C+C DPC C S+
Sbjct: 55 KATEGGNGCKCGSSCKC-DPCNCCSAST 81


>sp|Q43512|MT2X_SOLLC Metallothionein-like protein type 2 OS=Solanum
lycopersicum PE=3 SV=1
Length = 72

Score = 35.8 bits (81), Expect = 0.069
Identities = 15/23 (65%), Positives = 15/23 (65%)
Frame = +1

Query: 52 EVTVAGCACKCGSNCQCADPCPC 120
E AG CKCGSNC C DPC C
Sbjct: 51 EEKAAGEGCKCGSNCTC-DPCNC 72


>sp|Q40157|MT2A_SOLLC Metallothionein-like protein type 2 A
OS=Solanum lycopersicum GN=MTA PE=2 SV=1
Length = 72

Score = 35.8 bits (81), Expect = 0.069
Identities = 15/23 (65%), Positives = 15/23 (65%)
Frame = +1

Query: 52 EVTVAGCACKCGSNCQCADPCPC 120
E AG CKCGSNC C DPC C
Sbjct: 51 EEKAAGEGCKCGSNCTC-DPCNC 72


>sp|P43399|MT1_TRIRP Metallothionein-like protein 1 OS=Trifolium
repens GN=MT1B PE=3 SV=1
Length = 75

Score = 35.8 bits (81), Expect = 0.069
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1

Query: 76 CKCGSNCQCADPCPCAQTSSG 138
C CGS+C C D C C + SSG
Sbjct: 4 CNCGSSCNCGDSCKCNKRSSG 24



Score = 33.5 bits (75), Expect = 0.34
Identities = 14/27 (51%), Positives = 15/27 (55%)
Frame = +1

Query: 40 DAPREVTVAGCACKCGSNCQCADPCPC 120
DA V CKCGS+C C DPC C
Sbjct: 49 DAEMGVAAEDSGCKCGSSCTC-DPCNC 74


>sp|Q41670|MT1B_VICFA Metallothionein-like protein 1B OS=Vicia faba
GN=MT1B PE=3 SV=1
Length = 75

Score = 35.8 bits (81), Expect = 0.069
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1

Query: 76 CKCGSNCQCADPCPCAQTSSG 138
C CGS+C C D C C + SSG
Sbjct: 4 CNCGSSCNCGDSCKCNKRSSG 24



Score = 31.2 bits (69), Expect = 1.7
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCG NC C DPC C
Sbjct: 61 CKCGDNCTC-DPCNC 74


>sp|P43402|MT54_BRANA Metallothionein-like protein LSC54 OS=Brassica
napus PE=2 SV=1
Length = 45

Score = 35.4 bits (80), Expect = 0.089
Identities = 15/40 (37%), Positives = 18/40 (45%)
Frame = +1

Query: 1 CKCGKKVFDETALDAPREVTVAGCACKCGSNCQCADPCPC 120
CKCG E + + +C CGSNC C D C C
Sbjct: 12 CKCGDSCSCEKNYNTECD------SCSCGSNCSCGDSCSC 45



Score = 33.9 bits (76), Expect = 0.26
Identities = 13/31 (41%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = +1

Query: 61 VAGCACKCGSNCQCADPCPCAQ--TSSGDAC 147
+AG C CGS C+C D C C + + D+C
Sbjct: 1 MAGSNCGCGSGCKCGDSCSCEKNYNTECDSC 31


>sp|Q43513|MT2Y_SOLLC Metallothionein-like protein type 2 OS=Solanum
lycopersicum PE=3 SV=1
Length = 73

Score = 35.4 bits (80), Expect = 0.089
Identities = 14/23 (60%), Positives = 15/23 (65%)
Frame = +1

Query: 52 EVTVAGCACKCGSNCQCADPCPC 120
+ T G CKCGSNC C DPC C
Sbjct: 52 KATEGGNGCKCGSNCTC-DPCNC 73


>sp|P20830|MT1_PEA Metallothionein-like protein 1 OS=Pisum sativum
GN=MTA PE=3 SV=1
Length = 75

Score = 35.0 bits (79), Expect = 0.12
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1

Query: 76 CKCGSNCQCADPCPCAQTSSG 138
C CGS+C C D C C + SSG
Sbjct: 4 CGCGSSCNCGDSCKCNKRSSG 24



Score = 31.2 bits (69), Expect = 1.7
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCG NC C DPC C
Sbjct: 61 CKCGDNCTC-DPCNC 74


>sp|Q41669|MT1A_VICFA Metallothionein-like protein 1A OS=Vicia faba
GN=MT1A PE=3 SV=1
Length = 77

Score = 35.0 bits (79), Expect = 0.12
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1

Query: 76 CKCGSNCQCADPCPCAQTSSG 138
C CGS+C C D C C + SSG
Sbjct: 4 CGCGSSCNCGDSCKCNKRSSG 24



Score = 28.9 bits (63), Expect = 8.4
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCG C C DPC C
Sbjct: 64 CKCGDKCTC-DPCNC 77


tr_hit_id A8QMV1
Definition tr|A8QMV1|A8QMV1_AEQIR Metallothionein OS=Aequipecten irradians
Align length 49
Score (bit) 47.8
E-value 0.0003
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954806|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0021_G20, 5'
(365 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A8QMV1|A8QMV1_AEQIR Metallothionein OS=Aequipecten irradians ... 48 3e-04
tr|Q8S3M2|Q8S3M2_AZOFI Metallothionein-like protein 2 OS=Azolla ... 47 4e-04
tr|B3VKV7|B3VKV7_SOLNI Type 2 metallothionein OS=Solanum nigrum ... 40 0.076
tr|B3VKV6|B3VKV6_SOLNI Type 2 metallothionein OS=Solanum nigrum ... 39 0.099
tr|Q75NI4|Q75NI4_PHAAU Type 1 metallothionein OS=Phaseolus aureu... 33 0.11
tr|Q6PML5|Q6PML5_POPJC Metallothionein 1b (Putative uncharacteri... 33 0.14
tr|B3VKV5|B3VKV5_SOLNI Type 2 metallothionein OS=Solanum nigrum ... 39 0.17
tr|B5U0R0|B5U0R0_ANABR Metallothionein OS=Anadara broughtonii PE... 39 0.17
tr|A1Z0P1|A1Z0P1_9ROSI Metallothionein-like protein MT1A OS=Sali... 33 0.18
tr|Q2QKE5|Q2QKE5_CATRO Metallothionein OS=Catharanthus roseus PE... 38 0.29
tr|Q508T1|Q508T1_9ROSI Metallothionein-like protein OS=Betula pl... 37 0.38
tr|Q9ZSC6|Q9ZSC6_IPOBA Metallothionein-like type 1 protein OS=Ip... 37 0.49
tr|Q4ZGB4|Q4ZGB4_IPOBA Metallothionein-like type 1 protein OS=Ip... 37 0.49
tr|B8LFH0|B8LFH0_IPOBA Metallothionein-like protein OS=Ipomoea b... 37 0.49
tr|Q2VT53|Q2VT53_CAPAN Metallothionein-like protein type 2 OS=Ca... 37 0.64
tr|Q84SA5|Q84SA5_ASTTR Metallothionein 1 OS=Aster tripolium PE=4... 36 0.84
tr|O22492|O22492_OENJA Metallothionein-1 like protein OS=Oenanth... 36 0.84
tr|Q94I87|Q94I87_ATRBE Putative metallothionein-like protein typ... 36 1.1
tr|Q564N1|Q564N1_IPOBA Metallothionein-like type 2 protein OS=Ip... 36 1.1
tr|Q4ZGB3|Q4ZGB3_IPOBA Metallothionein-like type 2 protein OS=Ip... 36 1.1
tr|Q20BM7|Q20BM7_PANGI Metallothionein-like protein OS=Panax gin... 36 1.1
tr|B6ECQ7|B6ECQ7_HEVBR Metallothionein-like protein OS=Hevea bra... 36 1.1
tr|A8DUB1|A8DUB1_SOLLC Metallothionein-like protein OS=Solanum l... 36 1.1
tr|Q71M48|Q71M48_BRAOL Metallothionein-like protein OS=Brassica ... 35 1.4
tr|A9YTZ3|A9YTZ3_SOLTU Metallothionein-like protein OS=Solanum t... 35 1.4
tr|A7NZ11|A7NZ11_VITVI Chromosome chr6 scaffold_3, whole genome ... 35 1.4
tr|Q9SP23|Q9SP23_MEDSA Type 1 metallothionein OS=Medicago sativa... 35 1.9
tr|Q9M698|Q9M698_BRAOL Metallothionein-like protein 1 OS=Brassic... 35 1.9
tr|Q9FSF3|Q9FSF3_TOBAC Metallothionein-like protein type 2 (Frag... 35 1.9
tr|Q75NH8|Q75NH8_VICFA Type 1 metallothionein OS=Vicia faba GN=M... 35 1.9

>tr|A8QMV1|A8QMV1_AEQIR Metallothionein OS=Aequipecten irradians
PE=2 SV=1
Length = 145

Score = 47.8 bits (112), Expect = 3e-04
Identities = 19/49 (38%), Positives = 24/49 (48%)
Frame = +1

Query: 1 CKCGKKVFDETALDAPREVTVAGCACKCGSNCQCADPCPCAQTSSGDAC 147
CK F + P V G ACKCG +C+C+D CPC +G C
Sbjct: 33 CKVNCACFSAKSSTCPDGVCQMGVACKCGGSCKCSDSCPCPNCKAGCKC 81


>tr|Q8S3M2|Q8S3M2_AZOFI Metallothionein-like protein 2 OS=Azolla
filiculoides GN=AzMT2 PE=4 SV=1
Length = 77

Score = 47.4 bits (111), Expect = 4e-04
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 25/65 (38%)
Frame = +1

Query: 1 CKCGKKVFDETALDAPREVTV-------AGC------------------ACKCGSNCQCA 105
C+CGK+ FDETA DAP EV V + C C CGSNC C
Sbjct: 14 CRCGKRSFDETAFDAPMEVEVTVFGNEGSNCGFDSSCGFESDAQATSQNGCSCGSNCTC- 72

Query: 106 DPCPC 120
+PC C
Sbjct: 73 NPCRC 77


>tr|B3VKV7|B3VKV7_SOLNI Type 2 metallothionein OS=Solanum nigrum
GN=MT2e PE=4 SV=1
Length = 77

Score = 39.7 bits (91), Expect = 0.076
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +1

Query: 46 PREVTVAGCACKCGSNCQCADPCPC 120
P+ T G CKCGSNC+C DPC C
Sbjct: 54 PKNATEGGNGCKCGSNCKC-DPCTC 77


>tr|B3VKV6|B3VKV6_SOLNI Type 2 metallothionein OS=Solanum nigrum
GN=MT2d PE=4 SV=1
Length = 77

Score = 39.3 bits (90), Expect = 0.099
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +1

Query: 46 PREVTVAGCACKCGSNCQCADPCPC 120
P+ T G CKCGSNC+C DPC C
Sbjct: 54 PKNATEGGNGCKCGSNCKC-DPCNC 77


>tr|Q75NI4|Q75NI4_PHAAU Type 1 metallothionein OS=Phaseolus aureus
GN=MET PE=4 SV=1
Length = 73

Score = 32.7 bits (73), Expect(2) = 0.11
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCGSNC C DPC C
Sbjct: 59 CKCGSNCTC-DPCNC 72



Score = 26.9 bits (58), Expect(2) = 0.11
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +1

Query: 67 GCACKCGSNCQC 102
G +C CGSNC C
Sbjct: 7 GSSCNCGSNCSC 18


>tr|Q6PML5|Q6PML5_POPJC Metallothionein 1b (Putative uncharacterized
protein) OS=Populus jackii GN=MT1b PE=4 SV=1
Length = 73

Score = 33.1 bits (74), Expect(2) = 0.14
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCGSNC C DPC C
Sbjct: 59 CKCGSNCTC-DPCTC 72



Score = 26.2 bits (56), Expect(2) = 0.14
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +1

Query: 67 GCACKCGSNCQC 102
G CKCGS+C+C
Sbjct: 7 GSDCKCGSDCKC 18


>tr|B3VKV5|B3VKV5_SOLNI Type 2 metallothionein OS=Solanum nigrum
GN=MT2c PE=4 SV=1
Length = 77

Score = 38.5 bits (88), Expect = 0.17
Identities = 15/25 (60%), Positives = 16/25 (64%)
Frame = +1

Query: 46 PREVTVAGCACKCGSNCQCADPCPC 120
P+ T G CKCGSNC C DPC C
Sbjct: 54 PKNATEGGNGCKCGSNCTC-DPCNC 77


>tr|B5U0R0|B5U0R0_ANABR Metallothionein OS=Anadara broughtonii PE=4
SV=1
Length = 75

Score = 38.5 bits (88), Expect = 0.17
Identities = 20/53 (37%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Frame = +1

Query: 1 CKCGKK-VFDETALDAPREVTVAGCACK-CGSNCQCADPCPCAQTSSGDA-CK 150
CKC + D D + AGC C CG C+C+ C C Q +G A CK
Sbjct: 13 CKCDESCASDNCRCDPAKCKCKAGCKCSGCGVKCKCSGTCDCGQNCTGPADCK 65


>tr|A1Z0P1|A1Z0P1_9ROSI Metallothionein-like protein MT1A OS=Salix
matsudana PE=4 SV=1
Length = 73

Score = 33.1 bits (74), Expect(2) = 0.18
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1

Query: 76 CKCGSNCQCADPCPC 120
CKCGSNC C DPC C
Sbjct: 59 CKCGSNCTC-DPCTC 72



Score = 25.8 bits (55), Expect(2) = 0.18
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +1

Query: 67 GCACKCGSNCQC 102
G CKCGS+C+C
Sbjct: 7 GFDCKCGSDCKC 18


>tr|Q2QKE5|Q2QKE5_CATRO Metallothionein OS=Catharanthus roseus PE=4
SV=1
Length = 73

Score = 37.7 bits (86), Expect = 0.29
Identities = 19/60 (31%), Positives = 23/60 (38%), Gaps = 20/60 (33%)
Frame = +1

Query: 1 CKCGKKVFDETALDAPREVTVAGCA--------------------CKCGSNCQCADPCPC 120
C CG K + + A + + G A CKCGSNC C DPC C
Sbjct: 15 CSCGSKKYQDLETSATTTIIIEGVAPKKMDFEESEKSFGAEGGHGCKCGSNCNC-DPCNC 73