DK954944
Clone id TST39A01NGRL0021_M17
Library
Length 576
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0021_M17. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GGCACTCCCGCCTCTACATCTTGACGAGCGACGGCTAGCAGGAGTCTAGAGTCAAAGAAT
GTATTCTGCGGAGTTTTTTAACAGATGTAACATAACACTTCCAGATGCTCTTCTTCAAGG
AGCTGGTCGTAGTCATGGAGGCAGATTCATACTTGCAAAATCAAGGGATGATGATTCTTA
TAGACTGCCTTTGTCAAAGAGTATGCATCTGAGGCATTTAAAAGATTCTCCAAGGACCTC
TTTAAGGAAAAGTAAAGGTATAATGAGGTCAGTAGCTAGTGTATCATCTGCTGAGGACTC
TGCAAATACGGAATATCTGGAGAACCAGCCCAAAGAAGGTGCCTCGGGGTTGTATTTTGG
GCAGCCAACACAAAAGGAGGAGGTGATCGCTATACGTGAAAGTGCTGTACGAGCAAGTCT
TATGAAGAAGCTAAGTGACATCAATCTTTATGGGCGCCATCTGCAAAGACAGTTACGTGA
AAAGGAAGATGCCTTATTGAAGTGCAAAAGTGAACTGGAAACCATGGAGTCTGATATCCA
GATCCTTGCAAGGTTGGCTCAAGAAATTTGCAAAAG
■■Homology search results ■■ -
sp_hit_id Q3V6T2
Definition sp|Q3V6T2|GRDN_HUMAN Girdin OS=Homo sapiens
Align length 58
Score (bit) 38.1
E-value 0.032
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954944|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0021_M17, 5'
(576 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q3V6T2|GRDN_HUMAN Girdin OS=Homo sapiens GN=CCDC88A PE=1 SV=2 38 0.032
sp|Q5SNZ0|GRDN_MOUSE Girdin OS=Mus musculus GN=Ccdc88a PE=1 SV=2 37 0.092
sp|Q5PQ23|ODFP2_XENLA Outer dense fiber protein 2 OS=Xenopus lae... 33 1.3
sp|Q5JIE3|PFDA1_PYRKO Prefoldin subunit alpha 1 OS=Pyrococcus ko... 32 1.7
sp|Q9JIL8|RAD50_RAT DNA repair protein RAD50 OS=Rattus norvegicu... 32 3.0
sp|P70388|RAD50_MOUSE DNA repair protein RAD50 OS=Mus musculus G... 32 3.0
sp|Q6CEY7|NDC80_YARLI Probable kinetochore protein NDC80 OS=Yarr... 31 3.9
sp|Q0BPL2|KUP_GRABC Probable potassium transport system protein ... 31 3.9
sp|Q92878|RAD50_HUMAN DNA repair protein RAD50 OS=Homo sapiens G... 31 5.1
sp|P05659|MYSN_ACACA Myosin-2 heavy chain, non muscle OS=Acantha... 30 6.6
sp|Q15042|RB3GP_HUMAN Rab3 GTPase-activating protein catalytic s... 30 8.6
sp|Q9Y5S2|MRCKB_HUMAN Serine/threonine-protein kinase MRCK beta ... 30 8.6
sp|O75185|AT2C2_HUMAN Calcium-transporting ATPase type 2C member... 30 8.6
sp|Q811D2|ANR26_MOUSE Ankyrin repeat domain-containing protein 2... 30 8.6
sp|Q99996|AKAP9_HUMAN A-kinase anchor protein 9 OS=Homo sapiens ... 30 8.6

>sp|Q3V6T2|GRDN_HUMAN Girdin OS=Homo sapiens GN=CCDC88A PE=1 SV=2
Length = 1871

Score = 38.1 bits (87), Expect = 0.032
Identities = 23/58 (39%), Positives = 33/58 (56%)
Frame = +2

Query: 392 IRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQE 565
++ + R L +L+D R L+++L EK + LL CK ELE ME + L RL QE
Sbjct: 240 MKRTESRQHLSVELADAKAKIRRLRQELEEKTEQLLDCKQELEQMEIE---LKRLQQE 294


>sp|Q5SNZ0|GRDN_MOUSE Girdin OS=Mus musculus GN=Ccdc88a PE=1 SV=2
Length = 1873

Score = 36.6 bits (83), Expect = 0.092
Identities = 22/58 (37%), Positives = 33/58 (56%)
Frame = +2

Query: 392 IRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQE 565
++ + R L +L+D R L+++L EK + LL CK ELE +E + L RL QE
Sbjct: 240 MKRTESRQHLSVELADAKAKIRRLRQELEEKTEQLLDCKQELEQIEVE---LKRLQQE 294


>sp|Q5PQ23|ODFP2_XENLA Outer dense fiber protein 2 OS=Xenopus laevis
GN=odf2 PE=2 SV=1
Length = 649

Score = 32.7 bits (73), Expect = 1.3
Identities = 22/67 (32%), Positives = 37/67 (55%)
Frame = +2

Query: 374 KEEVIAIRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILAR 553
++E R + L+KKLSD + HLQ +L E+E+ + ++L+T E D L++
Sbjct: 281 RQETETYRLMEQKEMLLKKLSDADTEKMHLQLKLHEQEEKVEDLLAQLKT-EKD---LSK 336

Query: 554 LAQEICK 574
A E+ K
Sbjct: 337 TASEVSK 343


>sp|Q5JIE3|PFDA1_PYRKO Prefoldin subunit alpha 1 OS=Pyrococcus
kodakaraensis GN=pfdA1 PE=3 SV=1
Length = 146

Score = 32.3 bits (72), Expect = 1.7
Identities = 14/37 (37%), Positives = 27/37 (72%)
Frame = +2

Query: 458 HLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
+L+++++E E+A+ K + L+ +E+ + LAR AQEI
Sbjct: 98 YLEKRIKEYEEAIAKTQEALKKLEAQLGELARQAQEI 134


>sp|Q9JIL8|RAD50_RAT DNA repair protein RAD50 OS=Rattus norvegicus
GN=Rad50 PE=1 SV=1
Length = 1312

Score = 31.6 bits (70), Expect = 3.0
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +2

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
+LY H QR +REKE L+ C+ ELE + + ++L + E+
Sbjct: 306 DLYHNH-QRTVREKERRLVDCQRELEKLSKEARLLNQERAEL 346


>sp|P70388|RAD50_MOUSE DNA repair protein RAD50 OS=Mus musculus
GN=Rad50 PE=1 SV=1
Length = 1312

Score = 31.6 bits (70), Expect = 3.0
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +2

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
+LY H QR +REKE L+ C+ ELE + + ++L + E+
Sbjct: 306 DLYHNH-QRTVREKERRLVDCQRELEKLNKEARLLNQEKAEL 346


>sp|Q6CEY7|NDC80_YARLI Probable kinetochore protein NDC80
OS=Yarrowia lipolytica GN=NDC80 PE=3 SV=1
Length = 631

Score = 31.2 bits (69), Expect = 3.9
Identities = 26/122 (21%), Positives = 56/122 (45%), Gaps = 1/122 (0%)
Frame = +2

Query: 191 LSKSMHLRHLKDSPRTSLRKSKGIMRXXXXXXXXEDSANTEYLENQPKEGASGLYFGQPT 370
+S+ H+ L+ R LR+++ + E+S NTE LENQ + S Q
Sbjct: 502 VSQKRHIGELEAKSRR-LRETRNALEDSASMNENENSQNTERLENQFVQAQSETALAQSQ 560

Query: 371 QKEEVIAIRESAVRASLMKKLS-DINLYGRHLQRQLREKEDALLKCKSELETMESDIQIL 547
K + ++++ + KLS ++ L + + ++ + ++ K+ ++T D ++
Sbjct: 561 LKGLQVRLQDAEHERN---KLSVEVQQARERLSQDILKQAEVMVAFKAMVQTKLQDFEMF 617

Query: 548 AR 553
R
Sbjct: 618 VR 619


>sp|Q0BPL2|KUP_GRABC Probable potassium transport system protein kup
OS=Granulibacter bethesdensis (strain ATCC BAA-1260 /
CGDNIH1) GN=kup PE=3 SV=2
Length = 640

Score = 31.2 bits (69), Expect = 3.9
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = -1

Query: 489 SSFSRNCLCRWRP*RLMSLSFFIRLARTALSRIAITSSFCVGCPKYNPEA 340
S+ R + RWR L SF RL ++ + R+ T+ F G P Y P A
Sbjct: 459 SAGRRLLMSRWRQDSLPLASFLARLPQSRILRVPGTAVFLTGNPDYVPGA 508


>sp|Q92878|RAD50_HUMAN DNA repair protein RAD50 OS=Homo sapiens
GN=RAD50 PE=1 SV=1
Length = 1312

Score = 30.8 bits (68), Expect = 5.1
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = +2

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
+LY H QR +REKE L+ C ELE + + ++L + E+
Sbjct: 306 DLYHNH-QRTVREKERKLVDCHRELEKLNKESRLLNQEKSEL 346


>sp|P05659|MYSN_ACACA Myosin-2 heavy chain, non muscle OS=Acanthamoeba
castellanii PE=3 SV=1
Length = 1509

Score = 30.4 bits (67), Expect = 6.6
Identities = 20/57 (35%), Positives = 30/57 (52%)
Frame = +2

Query: 398 ESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
E+A SL KK D L R+LRE +DAL ++ ET+ S ++ R A ++
Sbjct: 994 EAAAHDSLKKKEED-------LSRELRETKDALADAENISETLRSKLKNTERGADDV 1043


tr_hit_id A7PRC0
Definition tr|A7PRC0|A7PRC0_VITVI Chromosome chr14 scaffold_26, whole genome shotgun sequence OS=Vitis vinifera
Align length 92
Score (bit) 61.2
E-value 4.0e-08
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK954944|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0021_M17, 5'
(576 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A7PRC0|A7PRC0_VITVI Chromosome chr14 scaffold_26, whole genom... 61 4e-08
tr|Q1M2Z4|Q1M2Z4_PLAAC Ampk protein (Fragment) OS=Platanus aceri... 60 9e-08
tr|Q6Z0Y8|Q6Z0Y8_ORYSJ Os02g0135900 protein OS=Oryza sativa subs... 59 2e-07
tr|B8AHD0|B8AHD0_ORYSI Putative uncharacterized protein OS=Oryza... 59 2e-07
tr|A3A2X1|A3A2X1_ORYSJ Putative uncharacterized protein OS=Oryza... 59 2e-07
tr|A9PE01|A9PE01_POPTR Putative uncharacterized protein OS=Popul... 56 2e-06
tr|B4FI49|B4FI49_MAIZE Putative uncharacterized protein OS=Zea m... 55 4e-06
tr|A9RYN0|A9RYN0_PHYPA Predicted protein OS=Physcomitrella paten... 53 1e-05
tr|Q94AX2|Q94AX2_ARATH AT5g39790/MKM21_80 OS=Arabidopsis thalian... 52 2e-05
tr|A9RQ51|A9RQ51_PHYPA Predicted protein (Fragment) OS=Physcomit... 49 2e-04
tr|Q5R4Y8|Q5R4Y8_PONAB Putative uncharacterized protein DKFZp459... 38 0.35
tr|A6QQ99|A6QQ99_BOVIN CCDC88A protein (Fragment) OS=Bos taurus ... 38 0.35
tr|Q9UI01|Q9UI01_HUMAN Putative uncharacterized protein OS=Homo ... 38 0.35
tr|Q86YH1|Q86YH1_HUMAN CCDC88A protein (Fragment) OS=Homo sapien... 38 0.35
tr|Q6PAV0|Q6PAV0_HUMAN CCDC88A protein (Fragment) OS=Homo sapien... 38 0.35
tr|Q6DCA5|Q6DCA5_HUMAN CCDC88A protein (Fragment) OS=Homo sapien... 38 0.35
tr|O14997|O14997_HUMAN Putative uncharacterized protein (Fragmen... 38 0.35
tr|B7ZM78|B7ZM78_HUMAN Putative uncharacterized protein OS=Homo ... 38 0.35
tr|Q0ZCE5|Q0ZCE5_POPTR Putative uncharacterized protein OS=Popul... 37 0.59
tr|Q22AC4|Q22AC4_TETTH Putative uncharacterized protein OS=Tetra... 37 0.59
tr|Q9W6V0|Q9W6V0_CHICK Hyperion protein, 419 kD isoform OS=Gallu... 36 1.3
tr|B4MNG1|B4MNG1_DROWI GK17057 OS=Drosophila willistoni GN=GK170... 36 1.3
tr|B7PAH4|B7PAH4_IXOSC CDC42 binding protein kinase alpha, putat... 35 2.3
tr|Q6E225|Q6E225_9PELO DNA-directed RNA polymerase (Fragment) OS... 35 2.9
tr|B6AIC7|B6AIC7_9CRYT Putative uncharacterized protein OS=Crypt... 34 5.0
tr|B8M9L8|B8M9L8_9EURO Spindle pole body associated protein SnaD... 34 5.0
tr|Q5TZ37|Q5TZ37_DANRE Novel protein similar to vertebrate CDC42... 34 6.5
tr|Q1E899|Q1E899_COCIM Predicted protein OS=Coccidioides immitis... 34 6.5

>tr|A7PRC0|A7PRC0_VITVI Chromosome chr14 scaffold_26, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00022432001
PE=4 SV=1
Length = 317

Score = 61.2 bits (147), Expect = 4e-08
Identities = 38/92 (41%), Positives = 51/92 (55%)
Frame = +2

Query: 299 SANTEYLENQPKEGASGLYFGQPTQKEEVIAIRESAVRASLMKKLSDINLYGRHLQRQLR 478
S N E+ P+E QP +E+ + + R L+KKLS+ N R L+RQL
Sbjct: 106 SDNENASEDSPEE-----LLDQPLGSDELKTLLVDSERTKLIKKLSEANQQNRILKRQLY 160

Query: 479 EKEDALLKCKSELETMESDIQILARLAQEICK 574
KEDAL+ KSEL ME ++Q L LA+EI K
Sbjct: 161 IKEDALVNFKSELAVMELEVQALVSLAEEIAK 192


>tr|Q1M2Z4|Q1M2Z4_PLAAC Ampk protein (Fragment) OS=Platanus
acerifolia GN=ampk PE=2 SV=1
Length = 205

Score = 60.1 bits (144), Expect = 9e-08
Identities = 31/81 (38%), Positives = 51/81 (62%)
Frame = +2

Query: 332 KEGASGLYFGQPTQKEEVIAIRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKS 511
++ +S + QP + + ++ + R L++KLS+ N + R L+RQL KEDAL+ KS
Sbjct: 99 EKDSSKEFLSQPLDGDGLKSLLADSERTKLIRKLSEANQHNRFLKRQLLIKEDALVNFKS 158

Query: 512 ELETMESDIQILARLAQEICK 574
EL +E +IQ L +LA+E+ K
Sbjct: 159 ELSVIELEIQALVKLAEEVAK 179


>tr|Q6Z0Y8|Q6Z0Y8_ORYSJ Os02g0135900 protein OS=Oryza sativa subsp.
japonica GN=OSJNBa0081C13.12 PE=4 SV=1
Length = 306

Score = 58.9 bits (141), Expect = 2e-07
Identities = 36/102 (35%), Positives = 58/102 (56%), Gaps = 10/102 (9%)
Frame = +2

Query: 293 EDSANTEYLENQPKEGAS--GLYFGQPTQK--------EEVIAIRESAVRASLMKKLSDI 442
ED + + P E S G +G P++ +E+ ++ + R+ L+KKLS+
Sbjct: 78 EDGPASSDSTSSPSEQTSDGGEVYGDPSENLNSRKLKSDELKSLLADSERSKLLKKLSEA 137

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
N Y R L+RQL+ K++ ++K KSEL ME ++Q L LA+EI
Sbjct: 138 NQYNRFLKRQLQMKDNDVVKFKSELAVMELELQALVALAEEI 179


>tr|B8AHD0|B8AHD0_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_05763 PE=4 SV=1
Length = 460

Score = 58.9 bits (141), Expect = 2e-07
Identities = 36/102 (35%), Positives = 58/102 (56%), Gaps = 10/102 (9%)
Frame = +2

Query: 293 EDSANTEYLENQPKEGAS--GLYFGQPTQK--------EEVIAIRESAVRASLMKKLSDI 442
ED + + P E S G +G P++ +E+ ++ + R+ L+KKLS+
Sbjct: 232 EDGPASSDSTSSPSEQTSDGGEVYGDPSENLNSRKLKSDELKSLLADSERSKLLKKLSEA 291

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
N Y R L+RQL+ K++ ++K KSEL ME ++Q L LA+EI
Sbjct: 292 NQYNRFLKRQLQMKDNDVVKFKSELAVMELELQALVALAEEI 333


>tr|A3A2X1|A3A2X1_ORYSJ Putative uncharacterized protein OS=Oryza
sativa subsp. japonica GN=OsJ_005143 PE=4 SV=1
Length = 463

Score = 58.9 bits (141), Expect = 2e-07
Identities = 36/102 (35%), Positives = 58/102 (56%), Gaps = 10/102 (9%)
Frame = +2

Query: 293 EDSANTEYLENQPKEGAS--GLYFGQPTQK--------EEVIAIRESAVRASLMKKLSDI 442
ED + + P E S G +G P++ +E+ ++ + R+ L+KKLS+
Sbjct: 235 EDGPASSDSTSSPSEQTSDGGEVYGDPSENLNSRKLKSDELKSLLADSERSKLLKKLSEA 294

Query: 443 NLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
N Y R L+RQL+ K++ ++K KSEL ME ++Q L LA+EI
Sbjct: 295 NQYNRFLKRQLQMKDNDVVKFKSELAVMELELQALVALAEEI 336


>tr|A9PE01|A9PE01_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 299

Score = 55.8 bits (133), Expect = 2e-06
Identities = 34/94 (36%), Positives = 53/94 (56%)
Frame = +2

Query: 293 EDSANTEYLENQPKEGASGLYFGQPTQKEEVIAIRESAVRASLMKKLSDINLYGRHLQRQ 472
++ +++E L +P + Q +E+ A+ + R +L KKLS+ N R L+RQ
Sbjct: 89 DEGSSSEDLPEEPLD--------QSLSSDELKALLLDSQRKNLTKKLSEANQQNRFLKRQ 140

Query: 473 LREKEDALLKCKSELETMESDIQILARLAQEICK 574
L EDAL+ KSEL ME +IQ L L++EI +
Sbjct: 141 LHVMEDALVNFKSELAVMELEIQALVTLSEEIAQ 174


>tr|B4FI49|B4FI49_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 305

Score = 54.7 bits (130), Expect = 4e-06
Identities = 26/64 (40%), Positives = 45/64 (70%)
Frame = +2

Query: 377 EEVIAIRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARL 556
+E+ A+ + R+ L+++LS+ N Y R L+RQL+ K+DA++K K EL +E ++Q L L
Sbjct: 115 DELKALLADSERSRLLRRLSEANQYNRFLKRQLQIKDDAVVKFKGELAVLELEMQALVGL 174

Query: 557 AQEI 568
A+E+
Sbjct: 175 AEEV 178


>tr|A9RYN0|A9RYN0_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_161880 PE=4 SV=1
Length = 312

Score = 52.8 bits (125), Expect = 1e-05
Identities = 25/53 (47%), Positives = 36/53 (67%)
Frame = +2

Query: 410 RASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
+ L+ KLS N Y R LQ LR+KE+AL+K +SEL M+ ++Q+L R+ EI
Sbjct: 131 KLQLLNKLSVANQYIRQLQHDLRDKEEALVKTRSELSAMQMELQVLVRILHEI 183


>tr|Q94AX2|Q94AX2_ARATH AT5g39790/MKM21_80 OS=Arabidopsis thaliana
PE=2 SV=1
Length = 277

Score = 52.0 bits (123), Expect = 2e-05
Identities = 26/69 (37%), Positives = 43/69 (62%)
Frame = +2

Query: 362 QPTQKEEVIAIRESAVRASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQ 541
+P +E+ ++ R+ L+KKLS+ N R L+RQL+ +E + K+EL ME ++Q
Sbjct: 82 RPLTSDELKSLLIDTERSKLVKKLSEANQQNRFLKRQLKTQEHEITNIKTELALMELEVQ 141

Query: 542 ILARLAQEI 568
L +LA+EI
Sbjct: 142 ALVKLAEEI 150


>tr|A9RQ51|A9RQ51_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_45927 PE=4 SV=1
Length = 179

Score = 49.3 bits (116), Expect = 2e-04
Identities = 25/53 (47%), Positives = 35/53 (66%)
Frame = +2

Query: 410 RASLMKKLSDINLYGRHLQRQLREKEDALLKCKSELETMESDIQILARLAQEI 568
R+ L+ KLS+ N Y HLQ +L+ KED+L EL ME ++Q+L +AQEI
Sbjct: 1 RSKLLGKLSEANQYISHLQYELQLKEDSLTDTGKELSAMEMELQVLIDMAQEI 53