DK955225
Clone id TST39A01NGRL0022_I11
Library
Length 587
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0022_I11. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CCAGCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACA
CACACACAGCGTCTCCTCGTTCTATTGGCCTCCAGGTCTCCACAACTCATCACAGCGCGT
ATAGTACTTCGCTCAACATGATGATGAAGCTGAATACTGTGGATGAGCGCATGGGGCTCA
TGCTAAAAGGACGTCATTCAAACAATTTTTTTGAGAAAGGCATGCCCGCAGTCTGCCGGT
TCTCTTCAAGCACCAACCCTTACAAATTCAGCAAGATGATGAAGGTGAATGCAGACAGGA
GCATTATTTTAGCTACTCCTAATGCCAGCTATGCTGCTCCCTTGGAAGCCTCCTTTGGAT
CTTCCTTTATCATCCGTCCATTGGCAGAGATCTTGCGTGATTTAAACAAGAAGGTTCCAG
ACCAGGTTCTTCTAGGAAAACCTGGGAGTTCCCAGCAAAAACACATCCCCTGGTACCAAG
CAAATCGTATGCTCAGCTTTTATGCCCCAGGTTGGTGTGGTGAAGTGCGCGATATAACCT
TCTCTGCAGATGGAAAATTTGTAACGGTCATCTATCGTGTCACAATC
■■Homology search results ■■ -
sp_hit_id Q21921
Definition sp|Q21921|SIRT1_CAEEL NAD-dependent deacetylase SIR2 homolog OS=Caenorhabditis elegans
Align length 73
Score (bit) 32.7
E-value 1.4
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955225|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0022_I11, 5'
(587 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q21921|SIRT1_CAEEL NAD-dependent deacetylase SIR2 homolog OS=... 33 1.4
sp|Q6ZEZ5|NEK3_ORYSJ Serine/threonine-protein kinase Nek3 OS=Ory... 32 2.3
sp|Q5F3M0|MED24_CHICK Mediator of RNA polymerase II transcriptio... 32 3.1
sp|P27169|PON1_HUMAN Serum paraoxonase/arylesterase 1 OS=Homo sa... 31 4.0
sp|Q60L58|SIRT1_CAEBR NAD-dependent deacetylase SIR2 homolog OS=... 31 5.2

>sp|Q21921|SIRT1_CAEEL NAD-dependent deacetylase SIR2 homolog
OS=Caenorhabditis elegans GN=sir-2.1 PE=1 SV=1
Length = 607

Score = 32.7 bits (73), Expect = 1.4
Identities = 26/73 (35%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +3

Query: 357 GSSFIIRPLAEILRDLNKKVPDQVLLGKPGSSQQKHIPWYQANRMLSFYAPGWCGE-VRD 533
GSS +RP+A I ++K VP Q+L+ + + +P Y A+ L G C + +RD
Sbjct: 327 GSSLKVRPVALIPHCVDKNVP-QILINR------ESLPHYNADIELL----GNCDDIIRD 375

Query: 534 ITFSADGKFVTVI 572
I FS G F +I
Sbjct: 376 ICFSLGGSFTELI 388


>sp|Q6ZEZ5|NEK3_ORYSJ Serine/threonine-protein kinase Nek3 OS=Oryza
sativa subsp. japonica GN=NEK3 PE=2 SV=1
Length = 585

Score = 32.0 bits (71), Expect = 2.3
Identities = 23/90 (25%), Positives = 37/90 (41%), Gaps = 3/90 (3%)
Frame = +3

Query: 177 LMLKGRHSNNFFEKGMPAVCRFSSSTNPYKFSKMMKVNADRSIILATPNASYAAPLEASF 356
L LK + N F A C + K+SK DR + + A+ P
Sbjct: 265 LQLKSSPARNLFPDTNKASCSDDENNWKAKYSKSHSFKVDRIVKVDKVAANNGHPSSTGT 324

Query: 357 GSSF---IIRPLAEILRDLNKKVPDQVLLG 437
+ + +P+ E+L L +KV D+V+ G
Sbjct: 325 AKDYQELLKQPMDELLGQLTEKVVDEVIHG 354


>sp|Q5F3M0|MED24_CHICK Mediator of RNA polymerase II transcription
subunit 24 OS=Gallus gallus GN=MED24 PE=2 SV=1
Length = 986

Score = 31.6 bits (70), Expect = 3.1
Identities = 20/76 (26%), Positives = 39/76 (51%), Gaps = 1/76 (1%)
Frame = +3

Query: 252 TNPYKFSKMMKVNADRSIILATPNASYAAPLEASFGSSFIIR-PLAEILRDLNKKVPDQV 428
T P K +++ N + + IL++PN S ++ L AS + +R PL +L +L + +
Sbjct: 850 TRPSKLMRLLSSNEEDANILSSPNRSMSSSLSASQLHAVSMRDPLNRVLANLFLLI-SSI 908

Query: 429 LLGKPGSSQQKHIPWY 476
L K + + + W+
Sbjct: 909 LGSKTAGTHTQFVQWF 924


>sp|P27169|PON1_HUMAN Serum paraoxonase/arylesterase 1 OS=Homo
sapiens GN=PON1 PE=1 SV=2
Length = 355

Score = 31.2 bits (69), Expect = 4.0
Identities = 26/96 (27%), Positives = 41/96 (42%), Gaps = 1/96 (1%)
Frame = +3

Query: 84 IGLQVSTTHHSAYSTSLNMMMKLNTVDERMGLMLKGRHSNNFFEKGMPAVCRFSSSTNPY 263
+GL + H S+Y T LN + ++ V+ ++KG + + + +P F SS Y
Sbjct: 12 MGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDMEILPNGLAFISSGLKY 71

Query: 264 KFSKMMKVNADRSIILATPNASYAAPLEASF-GSSF 368
K N+ I+L N LE GS F
Sbjct: 72 PGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKF 107


>sp|Q60L58|SIRT1_CAEBR NAD-dependent deacetylase SIR2 homolog
OS=Caenorhabditis briggsae GN=sir-2.1 PE=3 SV=1
Length = 602

Score = 30.8 bits (68), Expect = 5.2
Identities = 25/73 (34%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +3

Query: 357 GSSFIIRPLAEILRDLNKKVPDQVLLGKPGSSQQKHIPWYQANRMLSFYAPGWCGE-VRD 533
GSS +RP+A I ++K VP Q+L+ + + +P Y A+ L G C + VRD
Sbjct: 318 GSSLKVRPVALIPHCVDKNVP-QILINR------ESLPHYNADIELL----GNCDDIVRD 366

Query: 534 ITFSADGKFVTVI 572
I ++ G F +I
Sbjct: 367 ICYALGGSFAEMI 379


tr_hit_id A9TQS9
Definition tr|A9TQS9|A9TQS9_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 93
Score (bit) 126.0
E-value 1.0e-27
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955225|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0022_I11, 5'
(587 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9TQS9|A9TQS9_PHYPA Predicted protein OS=Physcomitrella paten... 126 1e-27
tr|B8LN18|B8LN18_PICSI Putative uncharacterized protein OS=Picea... 124 4e-27
tr|A9NSX2|A9NSX2_PICSI Putative uncharacterized protein OS=Picea... 124 4e-27
tr|A9PGP3|A9PGP3_POPTR Putative uncharacterized protein OS=Popul... 113 7e-24
tr|Q9FIJ4|Q9FIJ4_ARATH Gb|AAF26028.1 (At5g47870) (Putative uncha... 111 3e-23
tr|Q8LAJ6|Q8LAJ6_ARATH Putative uncharacterized protein OS=Arabi... 111 3e-23
tr|B7ZZ71|B7ZZ71_MAIZE Putative uncharacterized protein OS=Zea m... 103 9e-21
tr|B6ST86|B6ST86_MAIZE SnRK1-interacting protein 1 OS=Zea mays P... 103 9e-21
tr|Q851Y4|Q851Y4_ORYSJ Putative uncharacterized protein OSJNBa00... 102 1e-20
tr|Q10AI2|Q10AI2_ORYSJ Os03g0851500 protein OS=Oryza sativa subs... 102 1e-20
tr|A3APT8|A3APT8_ORYSJ Putative uncharacterized protein OS=Oryza... 102 1e-20
tr|A2XP50|A2XP50_ORYSI Putative uncharacterized protein OS=Oryza... 102 1e-20
tr|A9TKB2|A9TKB2_PHYPA Predicted protein OS=Physcomitrella paten... 102 2e-20
tr|B6TP06|B6TP06_MAIZE SnRK1-interacting protein 1 OS=Zea mays P... 101 4e-20
tr|B6SJF4|B6SJF4_MAIZE SnRK1-interacting protein 1 OS=Zea mays P... 99 2e-19
tr|Q0J0F6|Q0J0F6_ORYSJ Os09g0512800 protein OS=Oryza sativa subs... 97 9e-19
tr|B8BDI1|B8BDI1_ORYSI Putative uncharacterized protein OS=Oryza... 97 9e-19
tr|A2ZVM7|A2ZVM7_ORYSJ Putative uncharacterized protein OS=Oryza... 97 9e-19
tr|Q9FVV7|Q9FVV7_ARATH Putative uncharacterized protein F3I17.4 ... 85 3e-15
tr|Q3E7S4|Q3E7S4_ARATH Uncharacterized protein At1g71310.3 OS=Ar... 85 3e-15
tr|A7Q7S8|A7Q7S8_VITVI Chromosome chr18 scaffold_61, whole genom... 83 1e-14
tr|A5C7M6|A5C7M6_VITVI Putative uncharacterized protein OS=Vitis... 83 1e-14
tr|B6TIS1|B6TIS1_MAIZE SnRK1-interacting protein 1 OS=Zea mays P... 80 8e-14
tr|Q9LEH5|Q9LEH5_HORVU SnRK1-interacting protein 1 OS=Hordeum vu... 77 5e-13
tr|Q8L4C2|Q8L4C2_ORYSJ Putative SnRK1-interacting protein 1 (Put... 77 5e-13
tr|Q69IP1|Q69IP1_ORYSJ Putative uncharacterized protein P0450E05... 77 5e-13
tr|B6TJX4|B6TJX4_MAIZE SnRK1-interacting protein 1 OS=Zea mays P... 75 2e-12
tr|B8A718|B8A718_ORYSI Putative uncharacterized protein OS=Oryza... 52 2e-05
tr|A7QTD0|A7QTD0_VITVI Chromosome chr1 scaffold_166, whole genom... 43 0.015
tr|B1XQW9|B1XQW9_SYNP2 Putative uncharacterized protein OS=Synec... 37 0.82

>tr|A9TQS9|A9TQS9_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_149136 PE=4 SV=1
Length = 152

Score = 126 bits (316), Expect = 1e-27
Identities = 63/93 (67%), Positives = 71/93 (76%), Gaps = 1/93 (1%)
Frame = +3

Query: 312 ATPNASYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLLGKPGSSQQ-KHIPWYQANR 488
A P ASYA PLEAS S I RPLAEI+RD+NK+VPD+VL + K+IPWY ANR
Sbjct: 6 AVPYASYAVPLEASKEQSLITRPLAEIMRDMNKRVPDKVLKTRSDEGVALKYIPWYHANR 65

Query: 489 MLSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
MLSFYAPGWCGEVR I FSADG V+V+YRVTI
Sbjct: 66 MLSFYAPGWCGEVRSIVFSADGTTVSVVYRVTI 98


>tr|B8LN18|B8LN18_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 195

Score = 124 bits (311), Expect = 4e-27
Identities = 58/92 (63%), Positives = 74/92 (80%)
Frame = +3

Query: 312 ATPNASYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLLGKPGSSQQKHIPWYQANRM 491
A PN++Y PL+ SS I RPLAEILRDLNK+VPD+V+ G G+ + +IPWY ANRM
Sbjct: 66 AVPNSNYVVPLDIPPASSCITRPLAEILRDLNKRVPDKVVKGD-GAVEANYIPWYHANRM 124

Query: 492 LSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
LSF+APGWCGE+R++ FSADG+ V+V+YRVTI
Sbjct: 125 LSFFAPGWCGELRNVIFSADGRSVSVVYRVTI 156


>tr|A9NSX2|A9NSX2_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 184

Score = 124 bits (311), Expect = 4e-27
Identities = 58/92 (63%), Positives = 74/92 (80%)
Frame = +3

Query: 312 ATPNASYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLLGKPGSSQQKHIPWYQANRM 491
A PN++Y PL+ SS I RPLAEILRDLNK+VPD+V+ G G+ + +IPWY ANRM
Sbjct: 66 AVPNSNYVVPLDIPPASSCITRPLAEILRDLNKRVPDKVVKGD-GAVEANYIPWYHANRM 124

Query: 492 LSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
LSF+APGWCGE+R++ FSADG+ V+V+YRVTI
Sbjct: 125 LSFFAPGWCGELRNVIFSADGRSVSVVYRVTI 156


>tr|A9PGP3|A9PGP3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 223

Score = 113 bits (283), Expect = 7e-24
Identities = 54/93 (58%), Positives = 71/93 (76%), Gaps = 3/93 (3%)
Frame = +3

Query: 318 PNASYAAPLEASFGSSF---IIRPLAEILRDLNKKVPDQVLLGKPGSSQQKHIPWYQANR 488
PN++Y PL+ S S++ I RPL+EILRDLNK++PD ++ KP +S IPW+ ANR
Sbjct: 80 PNSNYVVPLDNSLASAYTSCITRPLSEILRDLNKRIPDNII--KPPNSSSTLIPWFHANR 137

Query: 489 MLSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
MLSFYAPGWCGE+RD+ F+ +G VTV+YRVTI
Sbjct: 138 MLSFYAPGWCGEIRDVIFAENGS-VTVVYRVTI 169


>tr|Q9FIJ4|Q9FIJ4_ARATH Gb|AAF26028.1 (At5g47870) (Putative
uncharacterized protein At5g47870) OS=Arabidopsis
thaliana GN=At5g47870 PE=2 SV=1
Length = 199

Score = 111 bits (277), Expect = 3e-23
Identities = 54/96 (56%), Positives = 67/96 (69%), Gaps = 4/96 (4%)
Frame = +3

Query: 312 ATPNASYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLLG----KPGSSQQKHIPWYQ 479
A PN++Y P++ SS I RPL EILRDLNKK+PD ++ ++ IPWY
Sbjct: 55 AVPNSNYVVPIDKFSSSSSITRPLIEILRDLNKKIPDNIVKSHDPPSTSAATSGFIPWYH 114

Query: 480 ANRMLSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
ANRMLSFYAPGWCGEVRD+ FS +G VTV+YR+TI
Sbjct: 115 ANRMLSFYAPGWCGEVRDVIFSENGN-VTVVYRLTI 149


>tr|Q8LAJ6|Q8LAJ6_ARATH Putative uncharacterized protein
OS=Arabidopsis thaliana PE=2 SV=1
Length = 199

Score = 111 bits (277), Expect = 3e-23
Identities = 54/96 (56%), Positives = 67/96 (69%), Gaps = 4/96 (4%)
Frame = +3

Query: 312 ATPNASYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLLG----KPGSSQQKHIPWYQ 479
A PN++Y P++ SS I RPL EILRDLNKK+PD ++ ++ IPWY
Sbjct: 55 AVPNSNYVVPIDKFSSSSSITRPLIEILRDLNKKIPDNIVKSHDPPSTSAATSGFIPWYH 114

Query: 480 ANRMLSFYAPGWCGEVRDITFSADGKFVTVIYRVTI 587
ANRMLSFYAPGWCGEVRD+ FS +G VTV+YR+TI
Sbjct: 115 ANRMLSFYAPGWCGEVRDVIFSENGN-VTVVYRLTI 149


>tr|B7ZZ71|B7ZZ71_MAIZE Putative uncharacterized protein OS=Zea mays
PE=2 SV=1
Length = 189

Score = 103 bits (256), Expect = 9e-21
Identities = 53/89 (59%), Positives = 65/89 (73%), Gaps = 2/89 (2%)
Frame = +3

Query: 327 SYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLL--GKPGSSQQKHIPWYQANRMLSF 500
+Y PL+A+ S I RPL EILRDLNK+VPD ++ + S+ IPWY ANRMLSF
Sbjct: 50 NYVVPLDAA--PSGITRPLVEILRDLNKRVPDAIVRPSSRRASASDPVIPWYHANRMLSF 107

Query: 501 YAPGWCGEVRDITFSADGKFVTVIYRVTI 587
YAPGWCGEVRD+ ++ GK VTVIYRVT+
Sbjct: 108 YAPGWCGEVRDVIYTDSGK-VTVIYRVTV 135


>tr|B6ST86|B6ST86_MAIZE SnRK1-interacting protein 1 OS=Zea mays PE=2
SV=1
Length = 189

Score = 103 bits (256), Expect = 9e-21
Identities = 53/89 (59%), Positives = 65/89 (73%), Gaps = 2/89 (2%)
Frame = +3

Query: 327 SYAAPLEASFGSSFIIRPLAEILRDLNKKVPDQVLL--GKPGSSQQKHIPWYQANRMLSF 500
+Y PL+A+ S I RPL EILRDLNK+VPD ++ + S+ IPWY ANRMLSF
Sbjct: 50 NYVVPLDAA--PSGITRPLVEILRDLNKRVPDAIVRPSSRRASASDPVIPWYHANRMLSF 107

Query: 501 YAPGWCGEVRDITFSADGKFVTVIYRVTI 587
YAPGWCGEVRD+ ++ GK VTVIYRVT+
Sbjct: 108 YAPGWCGEVRDVIYTDSGK-VTVIYRVTV 135


>tr|Q851Y4|Q851Y4_ORYSJ Putative uncharacterized protein
OSJNBa0015N08.17 OS=Oryza sativa subsp. japonica
GN=OSJNBa0015N08.17 PE=4 SV=1
Length = 156

Score = 102 bits (255), Expect = 1e-20
Identities = 60/134 (44%), Positives = 79/134 (58%), Gaps = 13/134 (9%)
Frame = +3

Query: 225 PAVCRFSSSTNPYKFSKMMKVNADRSIILATPNASYAAPLEASFGSSFII---------- 374
P R S+++ P + V A R + + T A P A+ S++++
Sbjct: 12 PVDTRLSAASLP------IVVRARRRVAVVTAAAPERKPAAAASSSNYVVVPLDAAPSGI 65

Query: 375 -RPLAEILRDLNKKVPDQVLLG--KPGSSQQKHIPWYQANRMLSFYAPGWCGEVRDITFS 545
RPL EILRDLNK+VPD V+ + S IPWY ANRMLSFYAPGWCGEVRD+ ++
Sbjct: 66 TRPLVEILRDLNKRVPDTVVRSSRRRASPSDPVIPWYHANRMLSFYAPGWCGEVRDVIYT 125

Query: 546 ADGKFVTVIYRVTI 587
+GK VTVIYRVT+
Sbjct: 126 DNGK-VTVIYRVTV 138


>tr|Q10AI2|Q10AI2_ORYSJ Os03g0851500 protein OS=Oryza sativa subsp.
japonica GN=Os03g0851500 PE=4 SV=1
Length = 192

Score = 102 bits (255), Expect = 1e-20
Identities = 60/134 (44%), Positives = 79/134 (58%), Gaps = 13/134 (9%)
Frame = +3

Query: 225 PAVCRFSSSTNPYKFSKMMKVNADRSIILATPNASYAAPLEASFGSSFII---------- 374
P R S+++ P + V A R + + T A P A+ S++++
Sbjct: 12 PVDTRLSAASLP------IVVRARRRVAVVTAAAPERKPAAAASSSNYVVVPLDAAPSGI 65

Query: 375 -RPLAEILRDLNKKVPDQVLLG--KPGSSQQKHIPWYQANRMLSFYAPGWCGEVRDITFS 545
RPL EILRDLNK+VPD V+ + S IPWY ANRMLSFYAPGWCGEVRD+ ++
Sbjct: 66 TRPLVEILRDLNKRVPDTVVRSSRRRASPSDPVIPWYHANRMLSFYAPGWCGEVRDVIYT 125

Query: 546 ADGKFVTVIYRVTI 587
+GK VTVIYRVT+
Sbjct: 126 DNGK-VTVIYRVTV 138