DK955356
Clone id TST39A01NGRL0022_O03
Library
Length 532
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0022_O03. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GTTCTCCAAAGCTATACCTAGTCATCTAGTCCCTCTTGTGCTGACGGCGGAGCTCTGCGT
GCTCGCTTCCTTCGATAGGAGTTCTTCACTTTTCGGGGCTAATCTAATCAATTTATGGCA
AGAGGAAGGAAGCTCAAGCGGGGGGGAGCTGCCAAGGATGCTGACAAAACGACAGACCCT
AAAGCCATGTATGATTCTGTTGACAGACCAAGCGGGGAGGTTGCACCTCATGGCAGCACC
CCCGAAGACGCTGTTGAAGCTCCTGATGAGGTGCAACCTGAGACGATGAAGAAGGGCTGC
CACAAAGAGCGACACGCCGCATTACACCCATGCGTATGGTGACATGACACCTGCACATAA
GGATACGCCCAACAAACATCTGCTAACGCGAAGGGGAAAGAAAAACAAGACATCTTCTGG
CGATACTCCTGTTCCGGAGGACAACATTACTGACTGTATCTGGACATCACTCTCATGTGC
GATTCCGAGACTAAGAATGATTGCTTCAAGTACCGTGTGTTTGGGCTTCCTG
■■Homology search results ■■ -
sp_hit_id Q8IZF2
Definition sp|Q8IZF2|GP116_HUMAN Probable G-protein coupled receptor 116 OS=Homo sapiens
Align length 49
Score (bit) 37.0
E-value 0.059
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955356|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0022_O03, 5'
(532 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q8IZF2|GP116_HUMAN Probable G-protein coupled receptor 116 OS... 37 0.059
sp|P20793|MAK_RAT Serine/threonine-protein kinase MAK OS=Rattus ... 34 0.39
sp|P53872|YNT0_YEAST Uncharacterized protein YNL190W OS=Saccharo... 33 1.1
sp|Q54CI8|DC1L1_DICDI Cytoplasmic dynein 1 light intermediate ch... 31 4.3
sp|Q8IWN7|RP1L1_HUMAN Retinitis pigmentosa 1-like 1 protein OS=H... 30 7.3
sp|Q0I6W0|NDHI_SYNS3 NAD(P)H-quinone oxidoreductase subunit I OS... 30 7.3
sp|Q5E284|ZNUC2_VIBF1 Zinc import ATP-binding protein znuC 2 OS=... 30 9.5
sp|Q9Y3S1|WNK2_HUMAN Serine/threonine-protein kinase WNK2 OS=Hom... 30 9.5
sp|Q8BHB9|CLIC6_MOUSE Chloride intracellular channel protein 6 O... 30 9.5

>sp|Q8IZF2|GP116_HUMAN Probable G-protein coupled receptor 116
OS=Homo sapiens GN=GPR116 PE=1 SV=2
Length = 1346

Score = 37.0 bits (84), Expect = 0.059
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -3

Query: 440 PPEQEYRQKMSCFSFPFALADVCWAYPYVQVSCHHTHGCNAACRSLWQP 294
P +E +K C+ F + V W V V CH T NAA S+W P
Sbjct: 610 PAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFT---NAANNSVWSP 655


>sp|P20793|MAK_RAT Serine/threonine-protein kinase MAK OS=Rattus
norvegicus GN=Mak PE=2 SV=1
Length = 622

Score = 34.3 bits (77), Expect = 0.39
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +1

Query: 154 KDADKTTDPKAMYDSVDRPSGEVAP-HGSTPEDAVEAPDE--VQPETMKKGCHKE 309
K + DPK + + +D+P+G+ P G P A++ P VQP ++G HK+
Sbjct: 313 KPSSSERDPKPLPNILDQPAGQPQPKQGHQPLQAIQPPQNTVVQPPPKQQGHHKQ 367


>sp|P53872|YNT0_YEAST Uncharacterized protein YNL190W
OS=Saccharomyces cerevisiae GN=YNL190W PE=1 SV=1
Length = 204

Score = 32.7 bits (73), Expect = 1.1
Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = +2

Query: 302 TKSDTPHYT--HAYGDMTPAHKD-TPNKHLLTRRGKKNKTSSGDTP 430
+KS TP++T H YG K TPN + GK NKTS TP
Sbjct: 58 SKSKTPNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTP 103



Score = 32.7 bits (73), Expect = 1.1
Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = +2

Query: 302 TKSDTPHYT--HAYGDMTPAHKD-TPNKHLLTRRGKKNKTSSGDTP 430
+KS TP++T H YG K TPN + GK NKTS TP
Sbjct: 78 SKSKTPNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTP 123



Score = 32.7 bits (73), Expect = 1.1
Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = +2

Query: 302 TKSDTPHYT--HAYGDMTPAHKD-TPNKHLLTRRGKKNKTSSGDTP 430
+KS TP++T H YG K TPN + GK NKTS TP
Sbjct: 98 SKSKTPNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTP 143



Score = 32.3 bits (72), Expect = 1.5
Identities = 18/44 (40%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = +2

Query: 302 TKSDTPHYTHAYGDMTPAHKD-TPNKHLLTRRGKKNKTSSGDTP 430
T + + H TH YG K TPN + GK NKTS TP
Sbjct: 40 TTTTSTHTTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTP 83


>sp|Q54CI8|DC1L1_DICDI Cytoplasmic dynein 1 light intermediate chain
1 OS=Dictyostelium discoideum GN=dync1li1 PE=3 SV=1
Length = 504

Score = 30.8 bits (68), Expect = 4.3
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2

Query: 107 INLWQEEGSSSGGELPRMLTKRQTLKPCMILLT 205
IN W EG +S +L + ++ +K CM+++T
Sbjct: 82 INYWSLEGEASQNDLLKFSLNKENIKNCMVIIT 114


>sp|Q8IWN7|RP1L1_HUMAN Retinitis pigmentosa 1-like 1 protein OS=Homo
sapiens GN=RP1L1 PE=1 SV=3
Length = 2480

Score = 30.0 bits (66), Expect = 7.3
Identities = 27/95 (28%), Positives = 39/95 (41%), Gaps = 5/95 (5%)
Frame = +1

Query: 1 VLQSYT*SSSPSCADGGALRARFLR*EFFTFRG*SNQFMARGRKLKRGGAAKDADKTTDP 180
+L +Y + + A GG LR + G + G + G + + D DP
Sbjct: 969 ILMTYELADETTGAAGGGLRGPEVD------PGDDHSLEGLGEPAQAGQQSLEGDPGQDP 1022

Query: 181 K---AMYDSVDR--PSGEVAPHGSTPEDAVEAPDE 270
+ A+ S D SGE P G+ PE EAP E
Sbjct: 1023 EPEGALLGSSDTGPQSGEGVPQGAAPEGVSEAPAE 1057


>sp|Q0I6W0|NDHI_SYNS3 NAD(P)H-quinone oxidoreductase subunit I
OS=Synechococcus sp. (strain CC9311) GN=ndhI PE=3 SV=1
Length = 219

Score = 30.0 bits (66), Expect = 7.3
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 4/50 (8%)
Frame = +1

Query: 169 TTDPKA--MYDSVDRPSGEVAPHGSTPE--DAVEAPDEVQPETMKKGCHK 306
TTDP + + V P+GEV PHG +P+ A + P+++ E G K
Sbjct: 150 TTDPSVQPLRELVYLPAGEVQPHGVSPDRPRAGKLPEQILEELKAAGSMK 199


>sp|Q5E284|ZNUC2_VIBF1 Zinc import ATP-binding protein znuC 2
OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=znuC2
PE=3 SV=1
Length = 243

Score = 29.6 bits (65), Expect = 9.5
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +2

Query: 119 QEEGSSSGGELPRMLTKRQTLKPCMILLTDQA 214
Q+ S SGG+L R+L R L+ M+LL D+A
Sbjct: 114 QQLNSLSGGQLQRVLLARALLEEPMLLLLDEA 145


>sp|Q9Y3S1|WNK2_HUMAN Serine/threonine-protein kinase WNK2 OS=Homo
sapiens GN=WNK2 PE=1 SV=4
Length = 2297

Score = 29.6 bits (65), Expect = 9.5
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +1

Query: 151 AKDADKTTDPKAMYDSVDRPSGEVAPHGSTPEDAVEAPDEVQP 279
A +A T D A + + P+ E +PH TP+ A+ P + P
Sbjct: 1496 APEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLP 1538


>sp|Q8BHB9|CLIC6_MOUSE Chloride intracellular channel protein 6
OS=Mus musculus GN=Clic6 PE=2 SV=1
Length = 596

Score = 29.6 bits (65), Expect = 9.5
Identities = 19/63 (30%), Positives = 25/63 (39%), Gaps = 5/63 (7%)
Frame = +1

Query: 145 GAAKDADKTTDPKAMYDSVDRPSGEVA-----PHGSTPEDAVEAPDEVQPETMKKGCHKE 309
G A+ D T P+A GE P G+ P+ A EAP Q + M G +
Sbjct: 73 GGAQAQDPRTGPEAETPGASGAPGEAEAAERDPEGAIPQGAEEAPSAQQVQGMSSGLDSQ 132

Query: 310 RHA 318
A
Sbjct: 133 GEA 135


tr_hit_id B8LQS0
Definition tr|B8LQS0|B8LQS0_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 22
Score (bit) 40.4
E-value 0.057
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955356|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0022_O03, 5'
(532 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B8LQS0|B8LQS0_PICSI Putative uncharacterized protein OS=Picea... 40 0.057
tr|B8LNW7|B8LNW7_PICSI Putative uncharacterized protein OS=Picea... 40 0.098
tr|A9NVJ6|A9NVJ6_PICSI Putative uncharacterized protein OS=Picea... 40 0.098
tr|A5BDJ5|A5BDJ5_VITVI Putative uncharacterized protein OS=Vitis... 39 0.17
tr|A7PU02|A7PU02_VITVI Chromosome chr7 scaffold_31, whole genome... 37 0.49
tr|Q5TF06|Q5TF06_HUMAN Novel transcript OS=Homo sapiens GN=RP3-3... 37 0.62
tr|B4DTV3|B4DTV3_HUMAN cDNA FLJ57199, highly similar to Probable... 37 0.62
tr|B4DLD2|B4DLD2_HUMAN cDNA FLJ57030, highly similar to Probable... 37 0.62
tr|A8K0D8|A8K0D8_HUMAN cDNA FLJ75412, highly similar to Homo sap... 37 0.62
tr|Q9FH52|Q9FH52_ARATH Genomic DNA, chromosome 5, TAC clone:K22G... 36 1.1
tr|Q8VZG6|Q8VZG6_ARATH AT5g61910/k22g18_30 (Putative uncharacter... 36 1.1
tr|Q3E873|Q3E873_ARATH Uncharacterized protein At5g61910.3 OS=Ar... 36 1.1
tr|B0DL79|B0DL79_LACBS Predicted protein OS=Laccaria bicolor (st... 35 1.8
tr|B0CUS5|B0CUS5_LACBS Predicted protein OS=Laccaria bicolor (st... 35 1.8
tr|A7QT80|A7QT80_VITVI Chromosome chr1 scaffold_166, whole genom... 35 3.2
tr|Q6AYW0|Q6AYW0_RAT Mak protein OS=Rattus norvegicus GN=Mak PE=... 34 4.1
tr|A7AQB3|A7AQB3_BABBO Variant erythrocyte surface antigen-1, be... 34 4.1
tr|B7XZR2|B7XZR2_RUMHA Putative uncharacterized protein OS=Blaut... 34 5.4
tr|A7NYZ0|A7NYZ0_VITVI Chromosome chr6 scaffold_3, whole genome ... 34 5.4
tr|A5CAT2|A5CAT2_VITVI Putative uncharacterized protein OS=Vitis... 34 5.4
tr|A5ADC6|A5ADC6_VITVI Putative uncharacterized protein OS=Vitis... 34 5.4
tr|Q7RUV4|Q7RUV4_NEUCR Putative uncharacterized protein OS=Neuro... 34 5.4
tr|A3TJB8|A3TJB8_9MICO Putative uncharacterized protein OS=Janib... 33 9.2
tr|A9S1G1|A9S1G1_PHYPA Predicted protein (Fragment) OS=Physcomit... 33 9.2
tr|B0W3X2|B0W3X2_CULQU Chymotrypsinogen OS=Culex quinquefasciatu... 33 9.2

>tr|B8LQS0|B8LQS0_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 394

Score = 40.4 bits (93), Expect = 0.057
Identities = 15/22 (68%), Positives = 19/22 (86%)
Frame = +1

Query: 466 ITLMCDSETKNDCFKYRVFGLP 531
+ MC+S+TK DCF+YRVFGLP
Sbjct: 114 LIFMCNSKTKQDCFRYRVFGLP 135


>tr|B8LNW7|B8LNW7_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 461

Score = 39.7 bits (91), Expect = 0.098
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1

Query: 457 YLDITLMCDSETKNDCFKYRVFGLP 531
+ + MC+S+TK +CF+YRVFGLP
Sbjct: 75 FAGLIFMCNSQTKQECFRYRVFGLP 99


>tr|A9NVJ6|A9NVJ6_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 458

Score = 39.7 bits (91), Expect = 0.098
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1

Query: 457 YLDITLMCDSETKNDCFKYRVFGLP 531
+ + MC+S+TK +CF+YRVFGLP
Sbjct: 72 FAGLIFMCNSQTKQECFRYRVFGLP 96


>tr|A5BDJ5|A5BDJ5_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_011005 PE=4 SV=1
Length = 468

Score = 38.9 bits (89), Expect = 0.17
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +1

Query: 475 MCDSETKNDCFKYRVFGLP 531
MC+ +TK DCFKYRVFGLP
Sbjct: 82 MCNGKTKPDCFKYRVFGLP 100


>tr|A7PU02|A7PU02_VITVI Chromosome chr7 scaffold_31, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00023604001
PE=4 SV=1
Length = 106

Score = 37.4 bits (85), Expect = 0.49
Identities = 21/55 (38%), Positives = 28/55 (50%)
Frame = +1

Query: 367 AQQTSANAKGKEKQDIFWRYSCSGGQHY*LYLDITLMCDSETKNDCFKYRVFGLP 531
A + S N K K K D+ + G I MC+S+TK DCF Y++ GLP
Sbjct: 8 ATKESENQKEKGKNDLRAEENIGG---------IIFMCNSKTKPDCFCYQIMGLP 53


>tr|Q5TF06|Q5TF06_HUMAN Novel transcript OS=Homo sapiens
GN=RP3-365O12.1-001 PE=2 SV=1
Length = 1346

Score = 37.0 bits (84), Expect = 0.62
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -3

Query: 440 PPEQEYRQKMSCFSFPFALADVCWAYPYVQVSCHHTHGCNAACRSLWQP 294
P +E +K C+ F + V W V V CH T NAA S+W P
Sbjct: 610 PAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFT---NAANNSVWSP 655


>tr|B4DTV3|B4DTV3_HUMAN cDNA FLJ57199, highly similar to Probable
G-protein coupled receptor 116 OS=Homo sapiens PE=2 SV=1
Length = 901

Score = 37.0 bits (84), Expect = 0.62
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -3

Query: 440 PPEQEYRQKMSCFSFPFALADVCWAYPYVQVSCHHTHGCNAACRSLWQP 294
P +E +K C+ F + V W V V CH T NAA S+W P
Sbjct: 165 PAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFT---NAANNSVWSP 210


>tr|B4DLD2|B4DLD2_HUMAN cDNA FLJ57030, highly similar to Probable
G-protein coupled receptor 116 OS=Homo sapiens PE=2 SV=1
Length = 770

Score = 37.0 bits (84), Expect = 0.62
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -3

Query: 440 PPEQEYRQKMSCFSFPFALADVCWAYPYVQVSCHHTHGCNAACRSLWQP 294
P +E +K C+ F + V W V V CH T NAA S+W P
Sbjct: 39 PAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFT---NAANNSVWSP 84


>tr|A8K0D8|A8K0D8_HUMAN cDNA FLJ75412, highly similar to Homo
sapiens G protein-coupled receptor 116 (GPR116), mRNA
OS=Homo sapiens PE=2 SV=1
Length = 1346

Score = 37.0 bits (84), Expect = 0.62
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -3

Query: 440 PPEQEYRQKMSCFSFPFALADVCWAYPYVQVSCHHTHGCNAACRSLWQP 294
P +E +K C+ F + V W V V CH T NAA S+W P
Sbjct: 610 PAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFT---NAANNSVWSP 655


>tr|Q9FH52|Q9FH52_ARATH Genomic DNA, chromosome 5, TAC clone:K22G18
OS=Arabidopsis thaliana PE=4 SV=1
Length = 776

Score = 36.2 bits (82), Expect = 1.1
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +1

Query: 475 MCDSETKNDCFKYRVFGLP 531
MC+ TK DC++YRVFG+P
Sbjct: 71 MCNGRTKTDCYRYRVFGIP 89