NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | ダウンロード | 利用許諾 | ヘルプ
AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK955499
Clone id TST39A01NGRL0023_E04
Library
Length 598
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0023_E04. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
GTCTGTTGAAAGCCGACACGCTGCTGCTATGGCGACCATCTCTCCCCCGCATGCTGCTGT
CGTCACCGTCTTCTAGGACCTTGCCGCACACAGTCACCCTCATGGAGGCCTCCTTTCTAT
TTTTCTGGCTGCTGTCGTCACACCTCCTGTAGCACCTCTTCTGCGAGCCCTCCTACTGCT
GGAAGCTACTCGGAGACAGAAATTATATCTGGGCATTGTGGAGATGGCAGCCCCTTCTTC
CCCTTGTCTCACTCGAACCAGAAAAAGGGCGCTGGACATTCCTCTTGCTTCTGGGGTGCC
TTCTCAATGTCTCACTAGAAGCAAGAAGAAGGCATTGGGCATCCCTCTCCTATTTGAAGG
AGGCGTTGATCCCAAGGCATGGGCCTTGGAGAGAGAGATGGCAAGAATCAGCAAGGAGTG
GAGCGATTTTCGCAAAGAGATAAAGATTGATCATCTTCCCTCTGAAAGCGATGACTATAA
GAAGGCCAGAGAAAAGATGCTGAGTTACAATGAACGAATGATCAATAAAATGCAAAAGAA
CCCTTTTATCTTTCCAGGAACAGTCTGTGAGATAGACGAAGGGGATGGGAATCGGTAT
■■Homology search results ■■ -
sp_hit_id P10645
Definition sp|P10645|CMGA_HUMAN Chromogranin-A OS=Homo sapiens
Align length 39
Score (bit) 34.7
E-value 0.38
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK955499|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0023_E04, 5'
         (598 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P10645|CMGA_HUMAN Chromogranin-A OS=Homo sapiens GN=CHGA PE=1...    35   0.38 
sp|Q4A1L3|BECN1_XENTR Beclin-1 OS=Xenopus tropicalis GN=becn1 PE...    32   1.9  
sp|Q6GP52|BECN1_XENLA Beclin-1 OS=Xenopus laevis GN=becn1 PE=2 SV=1    32   1.9  
sp|Q9XS63|CMGA_HORSE Chromogranin-A OS=Equus caballus GN=CHGA PE...    32   3.2  
sp|Q2INT9|DNLJ_ANADE DNA ligase OS=Anaeromyxobacter dehalogenans...    26   4.3  
sp|Q2RIM5|THIC1_MOOTA Thiamine biosynthesis protein thiC 1 OS=Mo...    31   5.5  
sp|P59556|RIBB_BUCBP 3,4-dihydroxy-2-butanone 4-phosphate syntha...    31   5.5  
sp|P50169|RDH3_RAT Retinol dehydrogenase 3 OS=Rattus norvegicus ...    30   7.1  
sp|Q86VM9|ZCH18_HUMAN Zinc finger CCCH domain-containing protein...    30   9.2  
sp|P55006|RDH7_RAT Retinol dehydrogenase 7 OS=Rattus norvegicus ...    30   9.3  
sp|O13713|NAB2_SCHPO Nuclear polyadenylated RNA-binding protein ...    30   9.3  
sp|Q0SM34|MURC_BORAP UDP-N-acetylmuramate--L-alanine ligase OS=B...    30   9.3  

>sp|P10645|CMGA_HUMAN Chromogranin-A OS=Homo sapiens GN=CHGA PE=1
           SV=7
          Length = 457

 Score = 34.7 bits (78), Expect = 0.38
 Identities = 18/39 (46%), Positives = 24/39 (61%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSE 466
           LF GG   K+  LE+E  R+SKEW D ++  K+D L  E
Sbjct: 316 LFRGG---KSGELEQEEERLSKEWEDSKRWSKMDQLAKE 351


>sp|Q4A1L3|BECN1_XENTR Beclin-1 OS=Xenopus tropicalis GN=becn1 PE=2
           SV=1
          Length = 445

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 19/58 (32%), Positives = 33/58 (56%)
 Frame = +2

Query: 365 VDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNERMINKMQK 538
           V  +A  LE+E AR  KE+S+F+++        E DD  K+ E  + Y +  ++K++K
Sbjct: 210 VREEAERLEQEEARYQKEYSEFKRQ------QLELDDDLKSVENQMRYAQIQLDKLKK 261


>sp|Q6GP52|BECN1_XENLA Beclin-1 OS=Xenopus laevis GN=becn1 PE=2 SV=1
          Length = 445

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 19/58 (32%), Positives = 33/58 (56%)
 Frame = +2

Query: 365 VDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNERMINKMQK 538
           V  +A  LE+E AR  KE+S+F+++        E DD  K+ E  + Y +  ++K++K
Sbjct: 210 VREEAERLEQEEARYQKEYSEFKRQ------QLELDDDLKSVENQMRYAQIQLDKLKK 261


>sp|Q9XS63|CMGA_HORSE Chromogranin-A OS=Equus caballus GN=CHGA PE=2
           SV=1
          Length = 448

 Score = 31.6 bits (70), Expect = 3.2
 Identities = 16/39 (41%), Positives = 22/39 (56%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSE 466
           LF GG   +    E+E  R+SKEW D ++  K+D L  E
Sbjct: 305 LFRGGKS-RELEQEKEQERLSKEWEDAKRWSKMDQLAKE 342


>sp|Q2INT9|DNLJ_ANADE DNA ligase OS=Anaeromyxobacter dehalogenans
           (strain 2CP-C) GN=ligA PE=3 SV=1
          Length = 687

 Score = 25.8 bits (55), Expect(2) = 4.3
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = +3

Query: 327 RRHWASLSYLKEALIPRHGPWRERWQE 407
           RR  + ++Y  EAL+P   PWR  W++
Sbjct: 226 RRPLSFVAY--EALVPGGDPWRTHWEK 250



 Score = 23.9 bits (50), Expect(2) = 4.3
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = +2

Query: 494 KMLSYNERMINKMQKNPFIFPGTVCEIDEGDGNR 595
           ++L+Y +RM  +  + P+   G V ++D+ D  R
Sbjct: 273 EVLAYRDRMAERRFELPYDTDGIVVKVDDLDWRR 306


>sp|Q2RIM5|THIC1_MOOTA Thiamine biosynthesis protein thiC 1
           OS=Moorella thermoacetica (strain ATCC 39073) GN=thiC1
           PE=3 SV=1
          Length = 432

 Score = 30.8 bits (68), Expect = 5.5
 Identities = 15/40 (37%), Positives = 26/40 (65%)
 Frame = +2

Query: 371 PKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAR 490
           P AW  +REMAR  K   D++++I++   P ++  Y++AR
Sbjct: 363 PGAWEWDREMARARKA-LDWQRQIELALDPEKARQYRRAR 401


>sp|P59556|RIBB_BUCBP 3,4-dihydroxy-2-butanone 4-phosphate synthase
           OS=Buchnera aphidicola subsp. Baizongia pistaciae
           GN=ribB PE=3 SV=1
          Length = 211

 Score = 30.8 bits (68), Expect = 5.5
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = +2

Query: 257 TRKRALDIPLASGVPSQCLTRSKKKALGIPLLFE 358
           T + AL I   SG+   C+T SK+K L +P++ +
Sbjct: 52  TEQMALTIRYGSGIVCLCITESKRKQLKLPMMVQ 85


>sp|P50169|RDH3_RAT Retinol dehydrogenase 3 OS=Rattus norvegicus
           GN=Rdh3 PE=1 SV=1
          Length = 317

 Score = 30.4 bits (67), Expect = 7.1
 Identities = 24/117 (20%), Positives = 46/117 (39%), Gaps = 18/117 (15%)
 Frame = +2

Query: 209 LGIVEMAAPSSPCLTRTRKRALDIPLASGVPS-----QCLTRSKKKALG----------- 340
           LG++E+     P + + R R ++I    G  S      C+++   +A             
Sbjct: 139 LGVIEVTLNMLPLVRKARGRVVNIASTMGRMSLVGGGYCISKYGVEAFSDSLRRELTYFG 198

Query: 341 --IPLLFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLS 505
             + ++  GG       +ER    + K W    +E+K  +     D Y KA E +++
Sbjct: 199 VKVAIIEPGGFKTNVTNMERLSDNLKKLWDQTTEEVKEIYGEKFQDSYMKAMESLVN 255


>sp|Q86VM9|ZCH18_HUMAN Zinc finger CCCH domain-containing protein 18
           OS=Homo sapiens GN=ZC3H18 PE=1 SV=1
          Length = 953

 Score = 30.0 bits (66), Expect = 9.2
 Identities = 27/79 (34%), Positives = 32/79 (40%)
 Frame = -3

Query: 503 SASFLWPSYSHRFQREDDQSLSLCENRSTPC*FLPSLSPRPMPWDQRLLQIGEGCPMPSS 324
           S S  + SYS R  R    S S   +RS      PS SP P P    +   GE  P P  
Sbjct: 574 SRSSSYSSYSSRSSRHSSFSGSRSRSRSFSS--SPSPSPTPSPHRPSIRTKGEPAPPPG- 630

Query: 323 CF**DIEKAPQKQEECPAP 267
                  KA +K  + PAP
Sbjct: 631 -------KAGEKSVKKPAP 642


>sp|P55006|RDH7_RAT Retinol dehydrogenase 7 OS=Rattus norvegicus
           GN=Rdh7 PE=2 SV=1
          Length = 317

 Score = 30.0 bits (66), Expect = 9.3
 Identities = 24/117 (20%), Positives = 46/117 (39%), Gaps = 18/117 (15%)
 Frame = +2

Query: 209 LGIVEMAAPSSPCLTRTRKRALDIPLASGVPS-----QCLTRSKKKALG----------- 340
           LG++E+     P + + R R ++I    G  S      C+++   +A             
Sbjct: 139 LGVIEVTLNMLPLVRKARGRVVNIASTMGRMSLLGGGYCISKYGVEAFSDSLRRELTYFG 198

Query: 341 --IPLLFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLS 505
             + ++  GG       +ER    + K W    +E+K  +     D Y KA E +++
Sbjct: 199 VKVAIIEPGGFKTNVTNMERLSDNLKKLWDQATEEVKEIYGEKFRDSYMKAMESLVN 255
tr_hit_id Q4R4V1
Definition tr|Q4R4V1|Q4R4V1_MACFA Brain cDNA, clone: QccE-12266, similar to human chromogranin A (parathyroid secretory protein 1) (CHGA),mRNA, RefSeq: NM_001275.2 OS=Macaca fascicularis
Align length 64
Score (bit) 37.0
E-value 0.87
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK955499|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0023_E04, 5'
         (598 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q4R4V1|Q4R4V1_MACFA Brain cDNA, clone: QccE-12266, similar to...    37   0.87 
tr|Q4J9S7|Q4J9S7_SULAC Bifunctional folate synthesis protein OS=...    35   2.5  
tr|A5DZF6|A5DZF6_LODEL Putative uncharacterized protein OS=Lodde...    35   3.3  
tr|Q8CYP3|Q8CYP3_STRR6 Putative uncharacterized protein spr1184 ...    35   4.3  
tr|Q04K19|Q04K19_STRP2 Putative uncharacterized protein OS=Strep...    35   4.3  
tr|Q1Q1K3|Q1Q1K3_9BACT Strongly similar to aspartyl/glutamyl-tRN...    35   4.3  
tr|Q86T07|Q86T07_HUMAN Full-length cDNA clone CS0DN001YP04 of Ad...    35   4.3  
tr|Q6NR84|Q6NR84_HUMAN Chromogranin A (Parathyroid secretory pro...    35   4.3  
tr|Q53FA8|Q53FA8_HUMAN Chromogranin A variant (Fragment) OS=Homo...    35   4.3  
tr|Q9ZPF8|Q9ZPF8_ARATH F5K24.9 protein OS=Arabidopsis thaliana G...    34   5.6  
tr|A9RPC2|A9RPC2_PHYPA Predicted protein OS=Physcomitrella paten...    34   7.3  
tr|Q9SKV5|Q9SKV5_ARATH F5J5.15 OS=Arabidopsis thaliana PE=4 SV=1       34   7.4  
tr|A6NE19|A6NE19_HUMAN Putative uncharacterized protein PPP1R12B...    34   7.4  
tr|A6D439|A6D439_9VIBR Putative uncharacterized protein OS=Vibri...    33   9.6  
tr|Q9HL64|Q9HL64_THEAC Hypothetical membrane protein OS=Thermopl...    33   9.6  

>tr|Q4R4V1|Q4R4V1_MACFA Brain cDNA, clone: QccE-12266, similar to
           human chromogranin A (parathyroid secretory protein 1)
           (CHGA),mRNA, RefSeq: NM_001275.2 OS=Macaca fascicularis
           PE=2 SV=1
          Length = 458

 Score = 37.0 bits (84), Expect = 0.87
 Identities = 22/64 (34%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
 Frame = +2

Query: 278 IPLASGVPSQCLTRSKKKALGIPL-LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDH 454
           +P   G       + +++  G P  LF GG   K+  LE+E  R+SKEW D ++  K+D 
Sbjct: 292 VPEGKGEQEYSQQKEEEEMAGAPQGLFRGG---KSGELEQEEERLSKEWEDSKRWSKMDQ 348

Query: 455 LPSE 466
           L  E
Sbjct: 349 LAKE 352


>tr|Q4J9S7|Q4J9S7_SULAC Bifunctional folate synthesis protein
           OS=Sulfolobus acidocaldarius GN=Saci_1101 PE=4 SV=1
          Length = 298

 Score = 35.4 bits (80), Expect = 2.5
 Identities = 19/51 (37%), Positives = 31/51 (60%)
 Frame = +2

Query: 362 GVDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNE 514
           GV+P+     +E++     W+D RK  K D LP E+ DYK  +++++SY E
Sbjct: 17  GVNPQEKETRQEVSIDVYYWADLRKGAKSDQLP-ETVDYKVLKKEIISYVE 66


>tr|A5DZF6|A5DZF6_LODEL Putative uncharacterized protein
           OS=Lodderomyces elongisporus GN=LELG_02743 PE=4 SV=1
          Length = 1439

 Score = 35.0 bits (79), Expect = 3.3
 Identities = 17/55 (30%), Positives = 29/55 (52%)
 Frame = +2

Query: 365 VDPKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNERMINK 529
           +D + + +ERE+ R ++EW +  + IKI      S    +A E+ML   E +  K
Sbjct: 460 LDQQYFVIERELIRTNREWENLSRHIKIAEESRRSRSEDRAYEEMLKSRESLEEK 514


>tr|Q8CYP3|Q8CYP3_STRR6 Putative uncharacterized protein spr1184
           OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
           GN=spr1184 PE=4 SV=1
          Length = 145

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 21/64 (32%), Positives = 31/64 (48%)
 Frame = +2

Query: 371 PKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNERMINKMQKNPFI 550
           P  W       +  K  +DF++EIK+ +    +D+ + A +K L  NER  N   K  FI
Sbjct: 79  PDGWYYRFHHTKYQKTQTDFQQEIKVYY----ADEPESAPQKGLYMNERYQNLKAKEAFI 134

Query: 551 FPGT 562
            P T
Sbjct: 135 CPKT 138


>tr|Q04K19|Q04K19_STRP2 Putative uncharacterized protein
           OS=Streptococcus pneumoniae serotype 2 (strain D39 /
           NCTC 7466) GN=SPD_1161 PE=4 SV=1
          Length = 145

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 21/64 (32%), Positives = 31/64 (48%)
 Frame = +2

Query: 371 PKAWALEREMARISKEWSDFRKEIKIDHLPSESDDYKKAREKMLSYNERMINKMQKNPFI 550
           P  W       +  K  +DF++EIK+ +    +D+ + A +K L  NER  N   K  FI
Sbjct: 79  PDGWYYRFHHTKYQKTQTDFQQEIKVYY----ADEPESAPQKGLYMNERYQNLKAKEAFI 134

Query: 551 FPGT 562
            P T
Sbjct: 135 CPKT 138


>tr|Q1Q1K3|Q1Q1K3_9BACT Strongly similar to
           aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit
           B OS=Candidatus Kuenenia stuttgartiensis GN=gatB PE=4
           SV=1
          Length = 480

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 19/79 (24%), Positives = 42/79 (53%), Gaps = 15/79 (18%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWS--DFRKEIKIDHLPSESDDYKKAREKMLSY----- 508
           + + G +PK    E+++A+IS EW   +   ++ + H P+  +DYKK ++  +++     
Sbjct: 397 MIDTGKEPKTIIEEKKLAQISDEWQIIEIVDKVIVSH-PNAIEDYKKGKKNAIAFIIGQV 455

Query: 509 --------NERMINKMQKN 541
                   N +++N++ KN
Sbjct: 456 MKESKGMANPKIVNEILKN 474


>tr|Q86T07|Q86T07_HUMAN Full-length cDNA clone CS0DN001YP04 of Adult
           brain of Homo sapiens (human) (Fragment) OS=Homo sapiens
           PE=2 SV=1
          Length = 367

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 18/39 (46%), Positives = 24/39 (61%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSE 466
           LF GG   K+  LE+E  R+SKEW D ++  K+D L  E
Sbjct: 226 LFRGG---KSGELEQEEERLSKEWEDSKRWSKMDQLAKE 261


>tr|Q6NR84|Q6NR84_HUMAN Chromogranin A (Parathyroid secretory
           protein 1) OS=Homo sapiens GN=CHGA PE=2 SV=1
          Length = 457

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 18/39 (46%), Positives = 24/39 (61%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSE 466
           LF GG   K+  LE+E  R+SKEW D ++  K+D L  E
Sbjct: 316 LFRGG---KSGELEQEEERLSKEWEDSKRWSKMDQLAKE 351


>tr|Q53FA8|Q53FA8_HUMAN Chromogranin A variant (Fragment) OS=Homo
           sapiens PE=2 SV=1
          Length = 457

 Score = 34.7 bits (78), Expect = 4.3
 Identities = 18/39 (46%), Positives = 24/39 (61%)
 Frame = +2

Query: 350 LFEGGVDPKAWALEREMARISKEWSDFRKEIKIDHLPSE 466
           LF GG   K+  LE+E  R+SKEW D ++  K+D L  E
Sbjct: 316 LFRGG---KSGELEQEEERLSKEWEDSKRWSKMDQLAKE 351


>tr|Q9ZPF8|Q9ZPF8_ARATH F5K24.9 protein OS=Arabidopsis thaliana
           GN=F5K24.9 PE=4 SV=1
          Length = 901

 Score = 34.3 bits (77), Expect = 5.6
 Identities = 26/104 (25%), Positives = 42/104 (40%), Gaps = 11/104 (10%)
 Frame = +2

Query: 212 GIVEMAAPSSPCLTRTRKRALDIPLASGVPSQCLTRSKKKALGIPLLFEGGVDPKAWALE 391
           G+V +  P +P +   ++RA    +A  VPS      + + +GI    E  VD     LE
Sbjct: 67  GVVPVQTPDAPRVVEVQQRAA---VAEAVPSYLRMIEQLQRIGIGCPVENRVDLAVHFLE 123

Query: 392 REM-----------ARISKEWSDFRKEIKIDHLPSESDDYKKAR 490
            +             +    W+DF  E    + P E+ D  +AR
Sbjct: 124 GDAHLLWRSVTARRRQADMSWADFMAEFNAKYFPQEAFDLMEAR 167