DK955520
Clone id TST39A01NGRL0023_F03
Library
Length 646
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0023_F03. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CTCTGATCCGCCTTCTCTGCGTCCTCACCTGCATGCCAACCTCTTGACAATGTCCATCAC
TCTGCTGCAAGCTTCGCAATAGACGTTCAGCTGACAGAGAGATTCCCCAGGAGCTATGGG
TAAGCAGCAAAGCAGCCATGGCGAGCTCAGCAGCCTATCGCAGGGCGCCTGTAGCGATGG
AGGTACCTCCGGACGCACATGCAAGCTGGAGATGACACAGGAAGAGGCGATTGGCAAGGC
AATGGAGGAGGTTTCCGGAATGCAGCAGGCAGGCAGAAGCTTTCAGGGCAGGAAGAAAAG
GAGTTATGGCGGTGGAGCGGCTGCATGTGAAGCAGAGGGAGGCTGCACAAATACGTGGTC
TGTGGGTGGATATGGGAGGCTGCAAGGAGGTGGCAACAGCTTCGGAGGTGGTGGGATTGG
CGGAGGATGTCCACGAGATCGCCTTCAAAGGCATCGACTGCAGGTGGCAGGAAGTGCAAG
GATTCCGGATGCTTGGTGCGGTGAGGCGCGCCTGCGCGAGTGGGTGAGCTCCTGTGAGGT
GGAAGATGCGCTGCAGCCTCCGGGCCTCTTCTCTGCACGGGCAGCCCTCATCTCTGCAGG
CTCCTCTCCTGCTGCCACGCGTACGGTGGTCGTCCCGGCCCCCTGT
■■Homology search results ■■ -
sp_hit_id Q642B2
Definition sp|Q642B2|ZN672_RAT Zinc finger protein 672 OS=Rattus norvegicus
Align length 98
Score (bit) 35.0
E-value 0.34
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955520|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0023_F03, 5'
(646 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q642B2|ZN672_RAT Zinc finger protein 672 OS=Rattus norvegicus... 35 0.34
sp|Q99LH4|ZN672_MOUSE Zinc finger protein 672 OS=Mus musculus GN... 34 0.58
sp|Q9CZT5|VASN_MOUSE Vasorin OS=Mus musculus GN=Vasn PE=2 SV=2 34 0.75
sp|Q6MG48|BAT2_RAT Large proline-rich protein BAT2 OS=Rattus nor... 33 0.97
sp|P57058|HUNK_HUMAN Hormonally up-regulated neu tumor-associate... 32 2.2
sp|Q68UT7|HUNK_PANTR Hormonally up-regulated neu tumor-associate... 32 2.8
sp|P57679|EVC_HUMAN Ellis-van Creveld syndrome protein OS=Homo s... 32 2.8
sp|P81122|IRS2_MOUSE Insulin receptor substrate 2 OS=Mus musculu... 31 4.8
sp|Q5R807|FCHO2_PONAB FCH domain only protein 2 OS=Pongo abelii ... 31 4.9
sp|Q0JRZ9|FCHO2_HUMAN FCH domain only protein 2 OS=Homo sapiens ... 31 4.9
sp|Q5ZMJ9|SRRM1_CHICK Serine/arginine repetitive matrix protein ... 31 6.4
sp|P77953|SSB_SHEHA Single-stranded DNA-binding protein OS=Shewa... 30 8.2
sp|Q13641|TPBG_HUMAN Trophoblast glycoprotein OS=Homo sapiens GN... 30 8.3

>sp|Q642B2|ZN672_RAT Zinc finger protein 672 OS=Rattus norvegicus
GN=Znf672 PE=2 SV=1
Length = 468

Score = 35.0 bits (79), Expect = 0.34
Identities = 29/98 (29%), Positives = 37/98 (37%), Gaps = 1/98 (1%)
Frame = -1

Query: 583 CP-CREEARRLQRIFHLTGAHPLAQARLTAPSIRNPCTSCHLQSMPLKAISWTSSANPTT 407
CP C R+ FHL AHP TAP+ P T H P S N +
Sbjct: 131 CPLCARSFRQSALPFHLARAHPPETTIATAPA---PSTLYHCTQCPRAFHSSAGLRNHSR 187

Query: 406 SEAVATSLQPPISTHRPRICAASLCFTCSRSTAITPFL 293
V + P HR +C S S+S+ +T L
Sbjct: 188 IHVVPSLSDPGAEAHRCGVCGKSF----SKSSTLTRHL 221


>sp|Q99LH4|ZN672_MOUSE Zinc finger protein 672 OS=Mus musculus
GN=Znf672 PE=2 SV=1
Length = 468

Score = 34.3 bits (77), Expect = 0.58
Identities = 30/98 (30%), Positives = 37/98 (37%), Gaps = 1/98 (1%)
Frame = -1

Query: 583 CP-CREEARRLQRIFHLTGAHPLAQARLTAPSIRNPCTSCHLQSMPLKAISWTSSANPTT 407
CP C R+ FHL AHP +TAPS P T H P S N +
Sbjct: 131 CPLCARSFRQSALPFHLARAHPPEIITVTAPS---PSTLYHCTQCPRAFHSSAGLRNHSR 187

Query: 406 SEAVATSLQPPISTHRPRICAASLCFTCSRSTAITPFL 293
V + P H IC S S+S+ +T L
Sbjct: 188 IHVVPSLSDPGTEAHLCGICGKSF----SKSSTLTRHL 221


>sp|Q9CZT5|VASN_MOUSE Vasorin OS=Mus musculus GN=Vasn PE=2 SV=2
Length = 673

Score = 33.9 bits (76), Expect = 0.75
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = -1

Query: 580 PCREEARRLQRIFHLTGAHPLAQARLTAPSIRNPCTSCHLQSMPLKAISWTSSANPTTSE 401
P + R L + + P+ T P+IR+ L S +A +W S PTT
Sbjct: 332 PPKNAGRLLLDLDYADFGCPVTTTTATVPTIRSTIREPTL-STSSQAPTWPSLTEPTTQA 390

Query: 400 AVATSLQPPI--STHRPRICAASLC 332
+ S PP +P+ C AS+C
Sbjct: 391 STVLSTAPPTMRPAPQPQDCPASIC 415


>sp|Q6MG48|BAT2_RAT Large proline-rich protein BAT2 OS=Rattus
norvegicus GN=Bat2 PE=1 SV=1
Length = 2161

Score = 33.5 bits (75), Expect = 0.97
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = -3

Query: 581 PVQRRGPEAAAHLPPHRSSPTRAGAPHRT 495
P + PE AH PP +S+PT+AG P T
Sbjct: 539 PTPEKDPEEPAHAPPVQSAPTQAGPPAPT 567


>sp|P57058|HUNK_HUMAN Hormonally up-regulated neu tumor-associated
kinase OS=Homo sapiens GN=HUNK PE=1 SV=1
Length = 714

Score = 32.3 bits (72), Expect = 2.2
Identities = 25/87 (28%), Positives = 33/87 (37%), Gaps = 1/87 (1%)
Frame = -3

Query: 569 RGPEAAAHLPPHRSSPTRAGAPHRTKHPESLHFLPPAVDAFEGDLVDILRQSHHLRSC-C 393
R P PH+ P G PH+ + P L + D V++L SHH R
Sbjct: 527 RTPRIVKKPEPHQPGPGSTGIPHK-EDPLMLDMVRSFESVDRDDHVEVLSPSHHYRILNS 585

Query: 392 HLLAASHIHPQTTYLCSLPLLHMQPLH 312
+ A + T LP M PLH
Sbjct: 586 PVSLARRNSSERTLSPGLPSGSMSPLH 612


>sp|Q68UT7|HUNK_PANTR Hormonally up-regulated neu tumor-associated
kinase OS=Pan troglodytes GN=HUNK PE=3 SV=1
Length = 714

Score = 32.0 bits (71), Expect = 2.8
Identities = 24/87 (27%), Positives = 33/87 (37%), Gaps = 1/87 (1%)
Frame = -3

Query: 569 RGPEAAAHLPPHRSSPTRAGAPHRTKHPESLHFLPPAVDAFEGDLVDILRQSHHLRSC-C 393
R P PH+ P G PH+ + P L + D +++L SHH R
Sbjct: 527 RTPRIVKKPEPHQPGPGSTGIPHK-EDPLMLDMVRSFESVDRDDHIEVLSPSHHYRILNS 585

Query: 392 HLLAASHIHPQTTYLCSLPLLHMQPLH 312
+ A + T LP M PLH
Sbjct: 586 PVSLARRNSSERTLSPGLPSGSMSPLH 612


>sp|P57679|EVC_HUMAN Ellis-van Creveld syndrome protein OS=Homo
sapiens GN=EVC PE=1 SV=1
Length = 992

Score = 32.0 bits (71), Expect = 2.8
Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
Frame = +3

Query: 198 HASWR*HRKRRLARQ-WRRFPECSRQAEAFRAGRKGVMAVERLHVKQREAAQIRGLWVDM 374
+A+W+ + R RQ W+ F E Q + + +V + H+++ IRG+ +
Sbjct: 564 NAAWQLGKSNRFRRQQWKLFQELLEQDQQVWMEECALSSVLQTHLREDHEGTIRGVLGRL 623

Query: 375 GGCKEVATASEVVG----LAEDVHEIAFKG 452
GG E +T + G L + +A +G
Sbjct: 624 GGLTEESTRCVLQGHDLLLRSALRRLALRG 653


>sp|P81122|IRS2_MOUSE Insulin receptor substrate 2 OS=Mus musculus
GN=Irs2 PE=1 SV=1
Length = 1321

Score = 31.2 bits (69), Expect = 4.8
Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 2/52 (3%)
Frame = -3

Query: 545 LPPHRSSP--TRAGAPHRTKHPESLHFLPPAVDAFEGDLVDILRQSHHLRSC 396
L + SSP RA + HR+ PES+ PPA D G+L + L C
Sbjct: 497 LDEYGSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGYMSMDRPLSHC 548


>sp|Q5R807|FCHO2_PONAB FCH domain only protein 2 OS=Pongo abelii
GN=FCHO2 PE=2 SV=1
Length = 810

Score = 31.2 bits (69), Expect = 4.9
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = -1

Query: 484 NPCTSCHLQSMPLKAISWTSSANPTTSEAVATSLQPPISTHRPRICAASL 335
+P L S +++ +SSA PTT +V T + PP RP++ + L
Sbjct: 428 DPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKL 477


>sp|Q0JRZ9|FCHO2_HUMAN FCH domain only protein 2 OS=Homo sapiens
GN=FCHO2 PE=1 SV=2
Length = 810

Score = 31.2 bits (69), Expect = 4.9
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = -1

Query: 484 NPCTSCHLQSMPLKAISWTSSANPTTSEAVATSLQPPISTHRPRICAASL 335
+P L S +++ +SSA PTT +V T + PP RP++ + L
Sbjct: 428 DPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKL 477


tr_hit_id Q7XVG6
Definition tr|Q7XVG6|Q7XVG6_ORYSJ Os04g0412100 protein OS=Oryza sativa subsp. japonica
Align length 70
Score (bit) 57.4
E-value 7.0e-07
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK955520|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0023_F03, 5'
(646 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|Q7XVG6|Q7XVG6_ORYSJ Os04g0412100 protein OS=Oryza sativa subs... 57 7e-07
tr|A3ATM9|A3ATM9_ORYSJ Putative uncharacterized protein OS=Oryza... 57 7e-07
tr|A7PPE2|A7PPE2_VITVI Chromosome chr8 scaffold_23, whole genome... 57 1e-06
tr|A5AR19|A5AR19_VITVI Putative uncharacterized protein OS=Vitis... 57 1e-06
tr|Q01JB4|Q01JB4_ORYSA H0717B12.8 protein OS=Oryza sativa GN=H07... 56 2e-06
tr|A2XT70|A2XT70_ORYSI Putative uncharacterized protein OS=Oryza... 56 2e-06
tr|A9PFK9|A9PFK9_POPTR Putative uncharacterized protein OS=Popul... 55 3e-06
tr|A7PKE5|A7PKE5_VITVI Chromosome chr15 scaffold_19, whole genom... 55 3e-06
tr|A5APW6|A5APW6_VITVI Putative uncharacterized protein OS=Vitis... 55 3e-06
tr|A9RF35|A9RF35_PHYPA Predicted protein OS=Physcomitrella paten... 52 3e-05
tr|B6SVC3|B6SVC3_MAIZE Putative uncharacterized protein OS=Zea m... 51 5e-05
tr|Q9LXJ1|Q9LXJ1_ARATH Putative uncharacterized protein F3C22_14... 50 9e-05
tr|Q9M280|Q9M280_ARATH Putative uncharacterized protein T22K7_13... 50 1e-04
tr|Q6ESE7|Q6ESE7_ORYSJ Os02g0532800 protein OS=Oryza sativa subs... 50 2e-04
tr|B3U2A7|B3U2A7_CUCSA Putative uncharacterized protein OS=Cucum... 50 2e-04
tr|A5AWV8|A5AWV8_VITVI Putative uncharacterized protein OS=Vitis... 50 2e-04
tr|A3A7L9|A3A7L9_ORYSJ Putative uncharacterized protein OS=Oryza... 50 2e-04
tr|A2X5N8|A2X5N8_ORYSI Putative uncharacterized protein OS=Oryza... 50 2e-04
tr|A9SFV2|A9SFV2_PHYPA Predicted protein OS=Physcomitrella paten... 47 8e-04
tr|B6ST07|B6ST07_MAIZE Putative uncharacterized protein OS=Zea m... 47 0.001
tr|B6SKB1|B6SKB1_MAIZE Putative uncharacterized protein OS=Zea m... 47 0.001
tr|A9NN02|A9NN02_PICSI Putative uncharacterized protein OS=Picea... 45 0.003
tr|A6NX78|A6NX78_9BACE Putative uncharacterized protein OS=Bacte... 39 0.21
tr|A9NKL9|A9NKL9_PICSI Putative uncharacterized protein OS=Picea... 37 1.3
tr|A8B5E8|A8B5E8_GIALA High cysteine membrane protein Group 5 OS... 37 1.3
tr|Q3ARG7|Q3ARG7_CHLCH Putative uncharacterized protein OS=Chlor... 36 2.3
tr|B6P720|B6P720_BRAFL Putative uncharacterized protein (Fragmen... 36 2.3
tr|Q1NGQ0|Q1NGQ0_9SPHN (P)ppGpp synthetase I (GTP pyrophosphokin... 35 3.0
tr|Q0KAB2|Q0KAB2_RALEH Transcriptional regulator, MocR family OS... 35 3.9
tr|Q1JT28|Q1JT28_TOXGO Putative uncharacterized protein OS=Toxop... 35 3.9

>tr|Q7XVG6|Q7XVG6_ORYSJ Os04g0412100 protein OS=Oryza sativa subsp.
japonica GN=OSJNBa0073L04.6 PE=2 SV=2
Length = 217

Score = 57.4 bits (137), Expect = 7e-07
Identities = 27/70 (38%), Positives = 39/70 (55%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR ++AG +PD W E L++WV + L PP L +AR AL++ +
Sbjct: 123 RERLKRHRREMAGRVWVPDMWGQEKLLKDWVDCAAFDRPLVPPDLLTARRALVAECCARR 182

Query: 614 ATRTVVVPAP 643
RT P P
Sbjct: 183 PDRTTTPPGP 192


>tr|A3ATM9|A3ATM9_ORYSJ Putative uncharacterized protein OS=Oryza
sativa subsp. japonica GN=OsJ_014151 PE=4 SV=1
Length = 193

Score = 57.4 bits (137), Expect = 7e-07
Identities = 27/70 (38%), Positives = 39/70 (55%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR ++AG +PD W E L++WV + L PP L +AR AL++ +
Sbjct: 99 RERLKRHRREMAGRVWVPDMWGQEKLLKDWVDCAAFDRPLVPPDLLTARRALVAECCARR 158

Query: 614 ATRTVVVPAP 643
RT P P
Sbjct: 159 PDRTTTPPGP 168


>tr|A7PPE2|A7PPE2_VITVI Chromosome chr8 scaffold_23, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00021619001
PE=4 SV=1
Length = 171

Score = 56.6 bits (135), Expect = 1e-06
Identities = 26/60 (43%), Positives = 39/60 (65%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR++VAGS IPD W E +++WV + +L + SARAAL++ G P+
Sbjct: 98 RERLKRHRVEVAGSVGIPDMWGQEELMKDWVDCSVFDASLVTSRIMSARAALVAEGRRPS 157


>tr|A5AR19|A5AR19_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_018276 PE=4 SV=1
Length = 196

Score = 56.6 bits (135), Expect = 1e-06
Identities = 26/60 (43%), Positives = 39/60 (65%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR++VAGS IPD W E +++WV + +L + SARAAL++ G P+
Sbjct: 98 RERLKRHRVEVAGSVGIPDMWGQEELMKDWVDCSVFDASLVTSRIMSARAALVAEGRRPS 157


>tr|Q01JB4|Q01JB4_ORYSA H0717B12.8 protein OS=Oryza sativa
GN=H0717B12.8 PE=4 SV=1
Length = 203

Score = 56.2 bits (134), Expect = 2e-06
Identities = 27/69 (39%), Positives = 39/69 (56%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR ++AG +PD W E L++WV + L PP L +AR AL++ +
Sbjct: 123 RERLKRHRREMAGRVWVPDMWGQEKLLKDWVDCAAFDRPLVPPDLLTARRALVAECCAHR 182

Query: 614 ATRTVVVPA 640
RT PA
Sbjct: 183 PDRTTTPPA 191


>tr|A2XT70|A2XT70_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_15808 PE=4 SV=1
Length = 203

Score = 56.2 bits (134), Expect = 2e-06
Identities = 27/69 (39%), Positives = 39/69 (56%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL+RHR ++AG +PD W E L++WV + L PP L +AR AL++ +
Sbjct: 123 RERLKRHRREMAGRVWVPDMWGQEKLLKDWVDCAAFDRPLVPPDLLTARRALVAECCARR 182

Query: 614 ATRTVVVPA 640
RT PA
Sbjct: 183 PDRTTTPPA 191


>tr|A9PFK9|A9PFK9_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 139

Score = 55.5 bits (132), Expect = 3e-06
Identities = 24/63 (38%), Positives = 39/63 (61%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALISAGSSPA 613
R+RL++HR +VAG IPD W E L++W+ ++ L P G+ SAR +L++ G +
Sbjct: 70 RERLKKHREEVAGRVMIPDTWGQEDSLKDWIDCSAFDELLAPNGISSARESLVAEGRKGS 129

Query: 614 ATR 622
+ R
Sbjct: 130 SPR 132


>tr|A7PKE5|A7PKE5_VITVI Chromosome chr15 scaffold_19, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00019133001
PE=4 SV=1
Length = 172

Score = 55.5 bits (132), Expect = 3e-06
Identities = 30/64 (46%), Positives = 42/64 (65%), Gaps = 1/64 (1%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDA-LQPPGLFSARAALISAGSSP 610
RDRL+RHR++VAG IPD W E +++W + C V DA L + SARAAL++ G P
Sbjct: 103 RDRLKRHRVEVAGRVWIPDIWGQEELMKDW-TDCSVFDASLVSSRIMSARAALMAEGRRP 161

Query: 611 AATR 622
++R
Sbjct: 162 NSSR 165


>tr|A5APW6|A5APW6_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_023275 PE=4 SV=1
Length = 164

Score = 55.5 bits (132), Expect = 3e-06
Identities = 30/64 (46%), Positives = 42/64 (65%), Gaps = 1/64 (1%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDA-LQPPGLFSARAALISAGSSP 610
RDRL+RHR++VAG IPD W E +++W + C V DA L + SARAAL++ G P
Sbjct: 95 RDRLKRHRVEVAGRVWIPDIWGQEELMKDW-TDCSVFDASLVSSRIMSARAALMAEGRRP 153

Query: 611 AATR 622
++R
Sbjct: 154 NSSR 157


>tr|A9RF35|A9RF35_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_158738 PE=4 SV=1
Length = 430

Score = 52.0 bits (123), Expect = 3e-05
Identities = 26/54 (48%), Positives = 37/54 (68%)
Frame = +2

Query: 434 RDRLQRHRLQVAGSARIPDAWCGEARLREWVSSCEVEDALQPPGLFSARAALIS 595
R+RL+R + S IP+ W GE +L+E+V+ +EDAL+P GL ARAAL+S
Sbjct: 351 RERLRRFSNEQCRSVVIPNQWGGETKLQEFVAFGNIEDALRPLGLMMARAALVS 404