DK956539
Clone id TST39A01NGRL0026_A03
Library
Length 654
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_A03. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
CGTAAACCGAGTCACGGCTCTGCTTATCGCTTAGACTTGCGCAGGCAGAGCTCTCTGCTG
CTGCAAGTCTAGGCTAAGCTTAGCTGGGCTCTCCCCTCGCTCTTCCAGGAGCCTGCTCTC
TCGCCTTTCTCCGATCTTGTGCCCTGTCGCGGTTTTTCTTTGTGCACCATTCTCAGGAAT
GGCTGCAATGGCAAAGTCTTGTCTTGACAAGACAACTGCTTTACAGGCCTCACCGTTTAG
GCAGGATGGAGTTGCCTCCTCAGCGAAGTGTGGACAACATGCAATTCTAAGCATGCCCCT
CCACCAGAGTAAAACCAGAAAGTTGAGACCTGCGAGTTTTGATAAGTTGCCAACACTAAG
TTTGCGCAAGGGGAAGGCTGTTTTGCCACATGGAACATGGAGGACTATATCAGCAATTGC
GGCTCCTATCGCACCGCCCCTGAGTGATCCTTCGTACAGAGCAAAGCTGGCGGAAGAGTA
TGGCTTTTCTCAAATTGGTGAGCCCGTTCCTCCAAATGTGACACTGAAGAATGTGATGGA
CTCTTTGCCTCCAGAGGTGTTTGAGATTAACAACATGAAGGCATGGAGGACTGTAGCTAT
CACGGTTACTGCCTATGCAGCAGGGCTTTTTATGATTGCAAAGGCACCCTGGTA
■■Homology search results ■■ -
sp_hit_id P48627
Definition sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus
Align length 65
Score (bit) 98.6
E-value 3.0e-20
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956539|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_A03, 5'
(654 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplast... 99 3e-20
sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplast... 96 2e-19
sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplast... 94 8e-19
sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplast... 92 2e-18
sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp. ... 39 0.031
sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensi... 37 0.090
sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43 OS=Arabid... 31 6.5

>sp|P48627|FAD6C_BRANA Omega-6 fatty acid desaturase, chloroplastic
OS=Brassica napus PE=2 SV=1
Length = 443

Score = 98.6 bits (244), Expect = 3e-20
Identities = 44/65 (67%), Positives = 57/65 (87%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 638 AKAPW 652
AKAPW
Sbjct: 140 AKAPW 144


>sp|P48628|FAD6C_SOYBN Omega-6 fatty acid desaturase, chloroplastic
OS=Glycine max PE=2 SV=1
Length = 424

Score = 95.5 bits (236), Expect = 2e-19
Identities = 42/66 (63%), Positives = 58/66 (87%)
Frame = +2

Query: 455 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 634
YR +LAE+YGF Q+GEP+ +VTLK+V++ LP EVFEI+++KAW++V I+VT+YA GLFM
Sbjct: 78 YRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVKAWKSVLISVTSYALGLFM 137

Query: 635 IAKAPW 652
I+KAPW
Sbjct: 138 ISKAPW 143


>sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplastic
OS=Spinacia oleracea GN=FAD6 PE=1 SV=1
Length = 447

Score = 93.6 bits (231), Expect = 8e-19
Identities = 41/63 (65%), Positives = 54/63 (85%)
Frame = +2

Query: 464 KLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAK 643
+LAE YGF QIGEP+P +VT+++++ SLP +VFEIN+ KAW TV I+VT+YA G+FMIAK
Sbjct: 87 QLAERYGFQQIGEPLPDDVTMRDIITSLPKQVFEINDTKAWGTVLISVTSYALGIFMIAK 146

Query: 644 APW 652
APW
Sbjct: 147 APW 149


>sp|P46312|FAD6C_ARATH Omega-6 fatty acid desaturase, chloroplastic
OS=Arabidopsis thaliana GN=FAD6 PE=1 SV=2
Length = 448

Score = 92.0 bits (227), Expect = 2e-18
Identities = 42/65 (64%), Positives = 55/65 (84%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KA ++V I+VT+Y GLFMI
Sbjct: 85 REQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKALKSVLISVTSYTLGLFMI 144

Query: 638 AKAPW 652
AK+PW
Sbjct: 145 AKSPW 149


>sp|P20388|DESA_SYNY3 Fatty acid desaturase OS=Synechocystis sp.
(strain PCC 6803) GN=desA PE=3 SV=1
Length = 351

Score = 38.5 bits (88), Expect = 0.031
Identities = 18/46 (39%), Positives = 27/46 (58%)
Frame = +2

Query: 515 NVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAKAPW 652
++ L++++ +LP E FE KAW +V IT+ A A G I PW
Sbjct: 23 DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPW 68


>sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensis
GN=desA PE=3 SV=1
Length = 351

Score = 37.0 bits (84), Expect = 0.090
Identities = 20/50 (40%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +2

Query: 506 VPPNVTLK-NVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMIAKAPW 652
VP ++ L+ +++++LP VF + KAW TV I V G IA APW
Sbjct: 18 VPSDLPLEEDIINTLPSGVFVQDRYKAWMTVIINVVMVGLGWLGIAIAPW 67


>sp|Q84WP6|NAC43_ARATH NAC domain-containing protein 43
OS=Arabidopsis thaliana GN=EMB2301 PE=2 SV=2
Length = 365

Score = 30.8 bits (68), Expect = 6.5
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = -2

Query: 218 QLSCQDKTLPLQPFLRMVHKEKPRQ-GTRSEKGERAGSWKSEGRAQLSLA*TCSSRELCL 42
Q C+ T P + HK+K GTR+ + AG WK+ GR ++ + + R + +
Sbjct: 62 QEMCKIGTTPQNDWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKIIYS---NGRRIGM 118

Query: 41 RKS 33
RK+
Sbjct: 119 RKT 121


tr_hit_id A9TJT1
Definition tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
Align length 65
Score (bit) 105.0
E-value 3.0e-21
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956539|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_A03, 5'
(654 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella paten... 105 3e-21
tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea... 101 3e-20
tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Popul... 101 5e-20
tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2... 99 3e-19
tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4... 98 4e-19
tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrat... 98 4e-19
tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplas... 98 5e-19
tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase OS=... 97 9e-19
tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase... 96 1e-18
tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase, c... 95 4e-18
tr|B8BB37|B8BB37_ORYSI Putative uncharacterized protein OS=Oryza... 95 4e-18
tr|A3BTJ3|A3BTJ3_ORYSJ Putative uncharacterized protein OS=Oryza... 95 4e-18
tr|B6TGK8|B6TGK8_MAIZE Putative uncharacterized protein OS=Zea m... 94 9e-18
tr|Q5U9G0|Q5U9G0_OLEEU Chloroplast fatty acid desaturase 6 OS=Ol... 90 1e-16
tr|Q9AXY4|Q9AXY4_BRAOL Omega 6 reductase (Fragment) OS=Brassica ... 84 6e-15
tr|A3QQP5|A3QQP5_MESVI Chloroplast omega-6 fatty acid desaturase... 82 4e-14
tr|Q9AXY2|Q9AXY2_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 78 4e-13
tr|Q9AXY3|Q9AXY3_BRACM Omega 6 reductase (Fragment) OS=Brassica ... 76 2e-12
tr|Q9AXY5|Q9AXY5_BRANA Omega 6 reductase (Fragment) OS=Brassica ... 75 3e-12
tr|Q9SBU4|Q9SBU4_CHLSW Chloroplast w6 desaturase OS=Chlamydomona... 71 5e-11
tr|Q9C600|Q9C600_ARATH Omega 6 reductase (Fragment) OS=Arabidops... 70 9e-11
tr|O48663|O48663_CHLRE Chloroplast w6 desaturase (Omega-6-FAD, c... 63 2e-08
tr|A9SI95|A9SI95_PHYPA Predicted protein (Fragment) OS=Physcomit... 60 2e-07
tr|A7R2X6|A7R2X6_VITVI Chromosome undetermined scaffold_462, who... 53 2e-05
tr|Q9ZSV8|Q9ZSV8_ACEAT Putative chloroplast desaturase (Fragment... 52 2e-05
tr|A9SCR5|A9SCR5_PHYPA Predicted protein (Fragment) OS=Physcomit... 52 4e-05
tr|Q55231|Q55231_SYNP2 Delta 12 desaturase OS=Synechococcus sp. ... 49 3e-04
tr|A0ZMU5|A0ZMU5_NODSP Fatty acid desaturase OS=Nodularia spumig... 45 0.005
tr|B0C0E1|B0C0E1_ACAM1 Fatty acid desaturase (Delta(12) desatura... 44 0.007
tr|B1WXI5|B1WXI5_CYAA5 Fatty acid desaturase OS=Cyanothece (stra... 44 0.009

>tr|A9TJT1|A9TJT1_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_222981 PE=4 SV=1
Length = 454

Score = 105 bits (261), Expect = 3e-21
Identities = 47/65 (72%), Positives = 58/65 (89%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAEEYGF+QIGEPVP +VTLK V+DS P EVFEIN++KAW+T+AI+V +Y+ GLFMI
Sbjct: 92 RQRLAEEYGFTQIGEPVPADVTLKKVIDSFPSEVFEINDLKAWKTIAISVASYSLGLFMI 151

Query: 638 AKAPW 652
AKAPW
Sbjct: 152 AKAPW 156


>tr|A9NV95|A9NV95_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 460

Score = 101 bits (252), Expect = 3e-20
Identities = 45/65 (69%), Positives = 58/65 (89%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +++E+YGF+QIGEPVP N+ LKNV+DSLP EVFEI++ KAW+TVAI+V AY+ GLF+I
Sbjct: 98 RNRISEKYGFTQIGEPVPDNIILKNVIDSLPQEVFEIDDTKAWKTVAISVAAYSLGLFLI 157

Query: 638 AKAPW 652
AKAPW
Sbjct: 158 AKAPW 162


>tr|A9PGN3|A9PGN3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 444

Score = 101 bits (251), Expect = 5e-20
Identities = 44/66 (66%), Positives = 58/66 (87%)
Frame = +2

Query: 455 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 634
YR +LAE YGF +IGEP+P N+TLK VMD+LP +VFEI++MKAW++V ++VT+YA GLFM
Sbjct: 81 YRKQLAESYGFRKIGEPLPDNITLKGVMDTLPKKVFEIDDMKAWKSVLVSVTSYAFGLFM 140

Query: 635 IAKAPW 652
I+KAPW
Sbjct: 141 ISKAPW 146


>tr|Q6DUV9|Q6DUV9_BRANA Omega-6 desaturase OS=Brassica napus PE=2
SV=1
Length = 443

Score = 98.6 bits (244), Expect = 3e-19
Identities = 44/65 (67%), Positives = 57/65 (87%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 638 AKAPW 652
AKAPW
Sbjct: 140 AKAPW 144


>tr|Q6DUV4|Q6DUV4_BRANA Omega-6 desaturase OS=Brassica napus PE=4
SV=1
Length = 443

Score = 98.2 bits (243), Expect = 4e-19
Identities = 44/65 (67%), Positives = 57/65 (87%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIG+ +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+YA GLFMI
Sbjct: 80 REQLAESYGFKQIGQNLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMI 139

Query: 638 AKAPW 652
AKAPW
Sbjct: 140 AKAPW 144


>tr|Q6DT48|Q6DT48_ARALP AT4G30950 (Fragment) OS=Arabidopsis lyrata
subsp. petraea PE=4 SV=1
Length = 203

Score = 98.2 bits (243), Expect = 4e-19
Identities = 44/65 (67%), Positives = 56/65 (86%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFMI
Sbjct: 50 REQLAESYGFKQIGENLPDNVTLKDIMDTLPKEVFEIDDLKAWKSVLISVTSYTLGLFMI 109

Query: 638 AKAPW 652
AKAPW
Sbjct: 110 AKAPW 114


>tr|Q09GN7|Q09GN7_DESSO Omega-6 fatty acid desaturase (Chloroplast
delta-12 fatty acid desaturase) OS=Descurainia sophia
GN=FAD6 PE=2 SV=1
Length = 447

Score = 97.8 bits (242), Expect = 5e-19
Identities = 44/65 (67%), Positives = 56/65 (86%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +LAE YGF QIGE +P NVTLK++MD+LP EVFEI+++KAW++V I+VT+Y GLFMI
Sbjct: 84 REQLAESYGFRQIGEDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISVTSYTLGLFMI 143

Query: 638 AKAPW 652
AKAPW
Sbjct: 144 AKAPW 148


>tr|A9UKE1|A9UKE1_OLEEU Plastidial delta-12 oleate desaturase
OS=Olea europaea GN=FAD6 PE=2 SV=1
Length = 443

Score = 97.1 bits (240), Expect = 9e-19
Identities = 42/65 (64%), Positives = 58/65 (89%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R +L+E YGF QIGEP+P NVTLK+++D+LP +VFEI+++KAW++V I+VT+YA G+FMI
Sbjct: 81 RKQLSENYGFRQIGEPLPDNVTLKDIVDTLPRKVFEIDDVKAWKSVLISVTSYALGIFMI 140

Query: 638 AKAPW 652
AKAPW
Sbjct: 141 AKAPW 145


>tr|A7XZU9|A7XZU9_9ROSI Chloroplast omega-6 fatty acid desaturase
OS=Jatropha curcas PE=2 SV=1
Length = 440

Score = 96.3 bits (238), Expect = 1e-18
Identities = 40/66 (60%), Positives = 57/66 (86%)
Frame = +2

Query: 455 YRAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFM 634
YR +LAE YGF QIGEP+P N+TL+ ++D+LP +VFEI+++KAW++V ++VT+YA GL M
Sbjct: 77 YRKQLAESYGFRQIGEPLPENITLREIIDTLPKKVFEIDDVKAWKSVLVSVTSYALGLLM 136

Query: 635 IAKAPW 652
I+KAPW
Sbjct: 137 ISKAPW 142


>tr|Q6Z9G4|Q6Z9G4_ORYSJ Putative Omega-6 fatty acid desaturase,
chloroplast OS=Oryza sativa subsp. japonica
GN=P0528B09.19 PE=4 SV=1
Length = 414

Score = 94.7 bits (234), Expect = 4e-18
Identities = 41/65 (63%), Positives = 56/65 (86%)
Frame = +2

Query: 458 RAKLAEEYGFSQIGEPVPPNVTLKNVMDSLPPEVFEINNMKAWRTVAITVTAYAAGLFMI 637
R ++ E+YGF QIGE +P NVTLK+VMDSLP EVFEI+++K+W +V I+VT+YA G+F+I
Sbjct: 52 RKQMCEDYGFKQIGEQLPDNVTLKDVMDSLPKEVFEIDDLKSWTSVLISVTSYALGIFLI 111

Query: 638 AKAPW 652
+KAPW
Sbjct: 112 SKAPW 116