DK956700
Clone id TST39A01NGRL0026_G20
Library
Length 408
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_G20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
AATAAGCAGGACCTTGTTTAGTTGTCTGTCCATGCATGCAGCAGCTTGCTCTGCTTTGTA
AAACACATCTTTGAACCTCGCTCTCAAGTGTAATACTGGCCTTCCTTTTACAACTTCGAA
TGGTTCATTCTTGTTTCGAAAAAAAAAAGAAGAAACATCAAAGAAGCCTCCATTATAAAA
AAAAATTAACAGTCACTTTAGAGAATCAACCCACGCCTGCCATGTCGTCTTTGAGAGTTG
AAAAAAAGAACAGCTTTTCAATTTGCGTGCGGCGGAATGGCAAGAGCTGGATCAAAAAAA
GGCGTCTTTGTCCCACACTTTGCAAGTCTTCAAGTCCAGGTATGCTGATGATGAGCCTCG
CAAGGGAAGTGACATTCTTGTTGAAGCTATGGAGAGGGAAGGGGTAGT
■■Homology search results ■■ -
sp_hit_id P09342
Definition sp|P09342|ILVB1_TOBAC Acetolactate synthase 1, chloroplastic OS=Nicotiana tabacum
Align length 37
Score (bit) 50.4
E-value 3.0e-06
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956700|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_G20, 5'
(408 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P09342|ILVB1_TOBAC Acetolactate synthase 1, chloroplastic OS=... 50 3e-06
sp|P09114|ILVB2_TOBAC Acetolactate synthase 2, chloroplastic OS=... 50 3e-06
sp|P27819|ILVB3_BRANA Acetolactate synthase 3, chloroplastic OS=... 47 2e-05
sp|P27818|ILVB1_BRANA Acetolactate synthase 1, chloroplastic OS=... 47 3e-05
sp|P17597|ILVB_ARATH Acetolactate synthase, chloroplastic OS=Ara... 44 2e-04
sp|P14874|ILVB2_BRANA Acetolactate synthase 2, chloroplastic OS=... 41 0.002
sp|Q41769|ILVB2_MAIZE Acetolactate synthase 2, chloroplastic OS=... 35 0.11
sp|Q6K2E8|ILVB1_ORYSJ Acetolactate synthase 1, chloroplastic OS=... 33 0.44
sp|Q9VS50|ECO_DROME N-acetyltransferase eco OS=Drosophila melano... 33 0.57
sp|Q41768|ILVB1_MAIZE Acetolactate synthase 1, chloroplastic OS=... 31 2.2
sp|Q6P1G2|JHD1B_MOUSE JmjC domain-containing histone demethylati... 30 3.7
sp|Q8NHM5|JHD1B_HUMAN JmjC domain-containing histone demethylati... 30 3.7
sp|P29461|PTP2_YEAST Tyrosine-protein phosphatase 2 OS=Saccharom... 30 4.8
sp|A8H993|MRAZ_SHEPA Protein mraZ OS=Shewanella pealeana (strain... 30 4.8
sp|Q3ZC98|NUP85_BOVIN Nucleoporin NUP85 OS=Bos taurus GN=NUP85 P... 29 8.5
sp|Q2NRB3|MLTC_SODGM Membrane-bound lytic murein transglycosylas... 29 8.5

>sp|P09342|ILVB1_TOBAC Acetolactate synthase 1, chloroplastic
OS=Nicotiana tabacum GN=ALS SURA PE=1 SV=1
Length = 667

Score = 50.4 bits (119), Expect = 3e-06
Identities = 23/37 (62%), Positives = 29/37 (78%)
Frame = +2

Query: 296 KKASLSHTLQVFKSRYADDEPRKGSDILVEAMEREGV 406
+K S + + F SR+A DEPRKGSD+LVEA+EREGV
Sbjct: 73 QKVSQTEKTETFVSRFAPDEPRKGSDVLVEALEREGV 109


>sp|P09114|ILVB2_TOBAC Acetolactate synthase 2, chloroplastic
OS=Nicotiana tabacum GN=ALS SURB PE=1 SV=1
Length = 664

Score = 50.1 bits (118), Expect = 3e-06
Identities = 23/37 (62%), Positives = 29/37 (78%)
Frame = +2

Query: 296 KKASLSHTLQVFKSRYADDEPRKGSDILVEAMEREGV 406
+K S + + F SR+A DEPRKGSD+LVEA+EREGV
Sbjct: 70 QKVSETQKAETFVSRFAPDEPRKGSDVLVEALEREGV 106


>sp|P27819|ILVB3_BRANA Acetolactate synthase 3, chloroplastic
OS=Brassica napus PE=3 SV=1
Length = 652

Score = 47.4 bits (111), Expect = 2e-05
Identities = 21/29 (72%), Positives = 26/29 (89%)
Frame = +2

Query: 320 LQVFKSRYADDEPRKGSDILVEAMEREGV 406
++ F SRYA DEPRKG+DILVEA+ER+GV
Sbjct: 66 IKTFISRYAPDEPRKGADILVEALERQGV 94


>sp|P27818|ILVB1_BRANA Acetolactate synthase 1, chloroplastic
OS=Brassica napus PE=3 SV=1
Length = 655

Score = 47.0 bits (110), Expect = 3e-05
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +2

Query: 323 QVFKSRYADDEPRKGSDILVEAMEREGV 406
+ F SRYA DEPRKG+DILVEA+ER+GV
Sbjct: 70 KTFVSRYAPDEPRKGADILVEALERQGV 97


>sp|P17597|ILVB_ARATH Acetolactate synthase, chloroplastic
OS=Arabidopsis thaliana GN=CSR 1.2 PE=1 SV=1
Length = 670

Score = 44.3 bits (103), Expect = 2e-04
Identities = 19/28 (67%), Positives = 25/28 (89%)
Frame = +2

Query: 323 QVFKSRYADDEPRKGSDILVEAMEREGV 406
+ F SR+A D+PRKG+DILVEA+ER+GV
Sbjct: 85 ETFISRFAPDQPRKGADILVEALERQGV 112


>sp|P14874|ILVB2_BRANA Acetolactate synthase 2, chloroplastic
OS=Brassica napus PE=3 SV=1
Length = 637

Score = 40.8 bits (94), Expect = 0.002
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +2

Query: 296 KKASLSHTLQVFKSRYADDEPRKGSDILVEAMEREGV 406
+ AS F S+YA + PR G+DILVEA+ER+GV
Sbjct: 52 RTASRRENPSTFSSKYAPNVPRSGADILVEALERQGV 88


>sp|Q41769|ILVB2_MAIZE Acetolactate synthase 2, chloroplastic OS=Zea
mays GN=ALS2 PE=3 SV=1
Length = 638

Score = 35.0 bits (79), Expect = 0.11
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2

Query: 341 YADDEPRKGSDILVEAMEREGV 406
+ +EPRKGSDILVEA+ER GV
Sbjct: 59 WGPNEPRKGSDILVEALERCGV 80


>sp|Q6K2E8|ILVB1_ORYSJ Acetolactate synthase 1, chloroplastic
OS=Oryza sativa subsp. japonica GN=ALS1 PE=2 SV=1
Length = 644

Score = 33.1 bits (74), Expect = 0.44
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = +2

Query: 353 EPRKGSDILVEAMEREGV 406
EPRKG+DILVEA+ER GV
Sbjct: 69 EPRKGADILVEALERCGV 86


>sp|Q9VS50|ECO_DROME N-acetyltransferase eco OS=Drosophila
melanogaster GN=eco PE=1 SV=1
Length = 1052

Score = 32.7 bits (73), Expect = 0.57
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = +3

Query: 87 SVILAFLLQLRMVHSCFEKKKKKHQRSLHYKKKLTVTLENQPTPAMSSLRVEKKNSFSIC 266
S + A L++ + S EK KKKH SL ++ T + +PA S ++K S S
Sbjct: 186 SKVAAMLMKGQDSRSMLEKSKKKHNHSLKTTAQVHTTKPKKTSPAEESQSDDEKPSSSKN 245

Query: 267 VRRN 278
R+N
Sbjct: 246 SRKN 249


>sp|Q41768|ILVB1_MAIZE Acetolactate synthase 1, chloroplastic OS=Zea
mays GN=ALS1 PE=3 SV=1
Length = 638

Score = 30.8 bits (68), Expect = 2.2
Identities = 13/18 (72%), Positives = 17/18 (94%)
Frame = +2

Query: 353 EPRKGSDILVEAMEREGV 406
+PRKG+DILVE++ER GV
Sbjct: 63 DPRKGADILVESLERCGV 80


tr_hit_id A9RWH6
Definition tr|A9RWH6|A9RWH6_PHYPA Acetolactate synthase OS=Physcomitrella patens subsp. patens
Align length 36
Score (bit) 50.4
E-value 4.0e-05
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956700|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_G20, 5'
(408 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9RWH6|A9RWH6_PHYPA Acetolactate synthase OS=Physcomitrella p... 50 4e-05
tr|A4S8I1|A4S8I1_OSTLU Acetolactate synthase OS=Ostreococcus luc... 50 4e-05
tr|Q7X9G4|Q7X9G4_9MAGN Acetolactate synthase OS=Papaver rhoeas G... 50 6e-05
tr|Q42767|Q42767_GOSHI Acetolactate synthase OS=Gossypium hirsut... 50 6e-05
tr|Q00U23|Q00U23_OSTTA Acetolactate synthase OS=Ostreococcus tau... 50 6e-05
tr|B8LK99|B8LK99_PICSI Putative uncharacterized protein OS=Picea... 50 6e-05
tr|A9S0R7|A9S0R7_PHYPA Acetolactate synthase OS=Physcomitrella p... 50 6e-05
tr|Q41716|Q41716_9ASTR Acetolactate synthase OS=Xanthium sp. PE=... 48 2e-04
tr|Q5VB49|Q5VB49_HELAN Acetolactate synthase OS=Helianthus annuu... 48 3e-04
tr|Q41717|Q41717_9ASTR Acetolactate synthase OS=Xanthium sp. PE=... 48 3e-04
tr|Q5VB48|Q5VB48_HELAN Acetolactate synthase OS=Helianthus annuu... 47 4e-04
tr|Q5VB47|Q5VB47_HELAN Acetolactate synthase OS=Helianthus annuu... 47 4e-04
tr|Q5VB46|Q5VB46_HELAN Acetolactate synthase OS=Helianthus annuu... 47 4e-04
tr|Q5VB45|Q5VB45_HELAN Acetolactate synthase OS=Helianthus annuu... 47 4e-04
tr|Q42768|Q42768_GOSHI Acetolactate synthase OS=Gossypium hirsut... 47 4e-04
tr|B5LAT2|B5LAT2_CAPAN Putative acetolactate synthase OS=Capsicu... 47 4e-04
tr|Q8L6E4|Q8L6E4_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6E3|Q8L6E3_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6E2|Q8L6E2_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6E1|Q8L6E1_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6E0|Q8L6E0_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D9|Q8L6D9_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D8|Q8L6D8_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D7|Q8L6D7_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D6|Q8L6D6_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D5|Q8L6D5_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|Q8L6D4|Q8L6D4_RAPRA Acetolactate synthase (Fragment) OS=Rapha... 47 6e-04
tr|A7Q4W4|A7Q4W4_VITVI Acetolactate synthase OS=Vitis vinifera G... 47 6e-04
tr|Q5VB44|Q5VB44_HELAN Acetolactate synthase OS=Helianthus annuu... 46 8e-04
tr|Q5VB43|Q5VB43_HELAN Acetolactate synthase OS=Helianthus annuu... 46 8e-04

>tr|A9RWH6|A9RWH6_PHYPA Acetolactate synthase OS=Physcomitrella
patens subsp. patens GN=PHYPADRAFT_120367 PE=3 SV=1
Length = 618

Score = 50.4 bits (119), Expect = 4e-05
Identities = 25/36 (69%), Positives = 29/36 (80%)
Frame = +2

Query: 299 KASLSHTLQVFKSRYADDEPRKGSDILVEAMEREGV 406
K + T + F SRYA+DEPRKGSDILVEA+EREGV
Sbjct: 25 KTLTAPTPKDFVSRYAEDEPRKGSDILVEALEREGV 60


>tr|A4S8I1|A4S8I1_OSTLU Acetolactate synthase OS=Ostreococcus
lucimarinus (strain CCE9901) GN=OSTLU_51596 PE=3 SV=1
Length = 610

Score = 50.4 bits (119), Expect = 4e-05
Identities = 23/26 (88%), Positives = 25/26 (96%)
Frame = +2

Query: 329 FKSRYADDEPRKGSDILVEAMEREGV 406
F SR+ADDEPRKGSDILVEA+EREGV
Sbjct: 13 FASRFADDEPRKGSDILVEALEREGV 38


>tr|Q7X9G4|Q7X9G4_9MAGN Acetolactate synthase OS=Papaver rhoeas
GN=als PE=2 SV=1
Length = 662

Score = 50.1 bits (118), Expect = 6e-05
Identities = 25/38 (65%), Positives = 29/38 (76%), Gaps = 4/38 (10%)
Frame = +2

Query: 305 SLSHTLQV----FKSRYADDEPRKGSDILVEAMEREGV 406
S +HT V F SRY DDEPRKG+D+LVEA+EREGV
Sbjct: 67 STTHTTSVSPPPFVSRYKDDEPRKGADVLVEALEREGV 104


>tr|Q42767|Q42767_GOSHI Acetolactate synthase OS=Gossypium hirsutum
GN=AHAS PE=3 SV=1
Length = 659

Score = 50.1 bits (118), Expect = 6e-05
Identities = 23/26 (88%), Positives = 25/26 (96%)
Frame = +2

Query: 329 FKSRYADDEPRKGSDILVEAMEREGV 406
F SRYADDEPRKG+DILVEA+EREGV
Sbjct: 76 FISRYADDEPRKGADILVEALEREGV 101


>tr|Q00U23|Q00U23_OSTTA Acetolactate synthase OS=Ostreococcus tauri
GN=Ot16g02270 PE=3 SV=1
Length = 679

Score = 50.1 bits (118), Expect = 6e-05
Identities = 22/26 (84%), Positives = 26/26 (100%)
Frame = +2

Query: 329 FKSRYADDEPRKGSDILVEAMEREGV 406
F+SR+ADDEPRKG+DILVEA+EREGV
Sbjct: 81 FQSRFADDEPRKGADILVEALEREGV 106


>tr|B8LK99|B8LK99_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 669

Score = 50.1 bits (118), Expect = 6e-05
Identities = 25/49 (51%), Positives = 31/49 (63%), Gaps = 5/49 (10%)
Frame = +2

Query: 275 EWQEL-----DQKKASLSHTLQVFKSRYADDEPRKGSDILVEAMEREGV 406
EW E D + S+ F SRYAD EPRKG+D++VEA+EREGV
Sbjct: 65 EWSESAVELKDPSQTSVKSLPSSFTSRYADSEPRKGADVIVEALEREGV 113


>tr|A9S0R7|A9S0R7_PHYPA Acetolactate synthase OS=Physcomitrella
patens subsp. patens GN=PHYPADRAFT_105543 PE=3 SV=1
Length = 583

Score = 50.1 bits (118), Expect = 6e-05
Identities = 23/24 (95%), Positives = 24/24 (100%)
Frame = +2

Query: 335 SRYADDEPRKGSDILVEAMEREGV 406
SRYADDEPRKGSDILVEA+EREGV
Sbjct: 2 SRYADDEPRKGSDILVEALEREGV 25


>tr|Q41716|Q41716_9ASTR Acetolactate synthase OS=Xanthium sp. PE=2
SV=1
Length = 648

Score = 48.1 bits (113), Expect = 2e-04
Identities = 23/43 (53%), Positives = 32/43 (74%), Gaps = 3/43 (6%)
Frame = +2

Query: 287 LDQKKASLSHT---LQVFKSRYADDEPRKGSDILVEAMEREGV 406
L K +++H+ + F SRYA D+PRKG+D+LVEA+EREGV
Sbjct: 48 LSDSKPTITHSPLPTESFISRYAPDQPRKGADVLVEALEREGV 90


>tr|Q5VB49|Q5VB49_HELAN Acetolactate synthase OS=Helianthus annuus
GN=AHAS1 PE=3 SV=1
Length = 655

Score = 47.8 bits (112), Expect = 3e-04
Identities = 21/29 (72%), Positives = 26/29 (89%)
Frame = +2

Query: 320 LQVFKSRYADDEPRKGSDILVEAMEREGV 406
+Q F SRYA D+PRKG+D+LVEA+EREGV
Sbjct: 69 VQPFVSRYAPDQPRKGADVLVEALEREGV 97


>tr|Q41717|Q41717_9ASTR Acetolactate synthase OS=Xanthium sp. PE=2
SV=1
Length = 648

Score = 47.8 bits (112), Expect = 3e-04
Identities = 23/43 (53%), Positives = 32/43 (74%), Gaps = 3/43 (6%)
Frame = +2

Query: 287 LDQKKASLSHT---LQVFKSRYADDEPRKGSDILVEAMEREGV 406
L K +++H+ + F SRYA D+PRKG+D+LVEA+EREGV
Sbjct: 48 LSDSKPTITHSPLPTKSFISRYAPDQPRKGADVLVEALEREGV 90