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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK956780
Clone id TST39A01NGRL0026_K04
Library
Length 555
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_K04. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
ACCGCGAGCTCCCAAGCAGTGGGAGGAGAATGAGATCTCTAACCGCGTGCCTGTTGAAGA
ATGAGCCGGCGACTTGTAGGCGGTGGCCCGGTTAAGGGGGAGAACCCCCGGAGCCGTAGC
GAAAGCGAGTCTTGCTGGGGCAATTGTCACCGCTTATAAACCCGAACCTGGGTGATCTAT
CCATGACCAGGATGAAGCTTGGGTGAAACTAAGTGGAGGTCCGAACCGACTGATGTTGAA
AAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGT
TCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGACTAGACCATCTAGGGGTAAAGCACCGT
TCCGGTGCGGGCTGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATGTAAC
GTTCAAGTAAAAGGAACGAAGGATAAGGGTCAACCAGTGAGACGATGAGGGATATGCTTC
ACCGTCGAGAGGGGAACAGCCCAGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGG
TAAAGGAGGTAGGAG
■■Homology search results ■■ -
sp_hit_id Q88AR1
Definition sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas syringae pv. tomato
Align length 43
Score (bit) 30.8
E-value 4.6
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956780|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_K04, 5'
         (555 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas s...    31   4.6  
sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas s...    31   4.6  
sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo s...    31   4.6  
sp|Q91Z22|PORIM_MOUSE Porimin OS=Mus musculus GN=Tmem123 PE=2 SV=1     31   4.7  
sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas s...    30   6.0  
sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas s...    30   6.0  
sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus ...    30   6.1  
sp|Q5R108|RECO_IDILO DNA repair protein recO OS=Idiomarina loihi...    30   7.9  

>sp|Q88AR1|GSH1_PSESM Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. tomato GN=gshA PE=3 SV=1
          Length = 529

 Score = 30.8 bits (68), Expect = 4.6
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +2

Query: 128 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 256
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 263 SLRKAVATPYKPYVDIGTHDQNGEWVQLN--TNVLQIENEYYS 303


>sp|Q48PX1|GSH1_PSE14 Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. phaseolicola (strain 1448A / Race 6)
           GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.8 bits (68), Expect = 4.6
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +2

Query: 128 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 256
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVDVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|Q99819|GDIR3_HUMAN Rho GDP-dissociation inhibitor 3 OS=Homo
           sapiens GN=ARHGDIG PE=2 SV=2
          Length = 225

 Score = 30.8 bits (68), Expect = 4.6
 Identities = 13/26 (50%), Positives = 20/26 (76%)
 Frame = +3

Query: 123 KRVLLGQLSPLINPNLGDLSMTRMKL 200
           KRVLLG L P ++P+L ++ +TR+ L
Sbjct: 73  KRVLLGPLPPAVDPSLPNVQVTRLTL 98


>sp|Q91Z22|PORIM_MOUSE Porimin OS=Mus musculus GN=Tmem123 PE=2 SV=1
          Length = 195

 Score = 30.8 bits (68), Expect = 4.7
 Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 19/69 (27%)
 Frame = -1

Query: 150 VTIAPARLAFATAPGVLPLNRATAYKS-------------------PAHSSTGTRLEISF 28
           +T+ PA +   + PGVLP    TA KS                   PAHSS  T + +S 
Sbjct: 80  ITVKPATIVKISTPGVLPHVTPTASKSTPNASASPNSTHTSASMTTPAHSSLLTTVTVSA 139

Query: 27  SSHCLGARG 1
           ++H    +G
Sbjct: 140 TTHPTKGKG 148


>sp|Q4ZZU4|GSH1_PSEU2 Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. syringae (strain B728a) GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +2

Query: 128 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 256
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|P61379|GSH1_PSESY Glutamate--cysteine ligase OS=Pseudomonas
           syringae pv. syringae GN=gshA PE=3 SV=1
          Length = 535

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +2

Query: 128 SLAGAIVTAYKPEPG*SIHDQDEAWVKLSGGPNRLMLKNQRMS 256
           SL  A+ T YKP      HDQ+  WV+L+   N L ++N+  S
Sbjct: 269 SLRKAVATPYKPYVEVGTHDQNGEWVQLN--TNVLQIENEYYS 309


>sp|A2A8U2|TM201_MOUSE Transmembrane protein 201 OS=Mus musculus
           GN=Tmem201 PE=2 SV=1
          Length = 664

 Score = 30.4 bits (67), Expect = 6.1
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -1

Query: 153 AVTIAPARLAFATAPGVLPLNRATAYKSPAHSS 55
           +VT +PA L   T PG LPL +   ++SP   S
Sbjct: 409 SVTSSPASLFIPTPPGFLPLTKQQLFRSPRRVS 441


>sp|Q5R108|RECO_IDILO DNA repair protein recO OS=Idiomarina
           loihiensis GN=recO PE=3 SV=1
          Length = 234

 Score = 30.0 bits (66), Expect = 7.9
 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 3/79 (3%)
 Frame = -2

Query: 389 LVPLSQPAPERCFTPRWSSQLLRLNAFRGEPASSGFEWHFTPNHNSSADFSTSVGSDLHL 210
           LVP    APE  F    S+     N+ R EPA   FEW    +  ++ D+     +   L
Sbjct: 99  LVPTEAEAPE-LFESYLSALQNMTNSERVEPALRQFEWQLLQHLGAAFDWHYDADTGNVL 157

Query: 209 VSPK---LHPGHG*ITQVR 162
              K     P HG +TQ R
Sbjct: 158 SDSKWCYFIPDHGFVTQAR 176
tr_hit_id Q5M9Z9
Definition tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b OS=Nicotiana tabacum
Align length 37
Score (bit) 77.4
E-value 1.0e-33
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956780|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_K04, 5'
         (555 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b ...    77   1e-33
tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa s...   102   2e-20
tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whol...    77   1e-19
tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medic...    96   1e-18
tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1            87   5e-16
tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein OS=Rumin...    79   1e-13
tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein OS=Rumin...    79   1e-13
tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein OS=Rumin...    77   8e-13
tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein OS=Rumin...    72   3e-12
tr|B1BC32|B1BC32_CLOBO Putative uncharacterized protein OS=Clost...    45   4e-10
tr|Q85WS9|Q85WS9_PINKO ORF40u OS=Pinus koraiensis PE=4 SV=2            65   2e-09
tr|A7PNF4|A7PNF4_VITVI Chromosome chr1 scaffold_22, whole genome...    65   2e-09
tr|A5KR91|A5KR91_9FIRM Putative uncharacterized protein OS=Rumin...    62   3e-09
tr|A7QY24|A7QY24_VITVI Chromosome undetermined scaffold_237, who...    52   9e-09
tr|B0A7G6|B0A7G6_9CLOT Putative uncharacterized protein OS=Clost...    62   2e-08
tr|A5KRH1|A5KRH1_9FIRM Putative uncharacterized protein OS=Rumin...    62   2e-08
tr|B2PX02|B2PX02_PROST Putative uncharacterized protein OS=Provi...    62   3e-08
tr|A7B5W8|A7B5W8_RUMGN Putative uncharacterized protein OS=Rumin...    57   6e-08
tr|Q0JIM3|Q0JIM3_ORYSJ Photosystem I P700 chlorophyll A apoprote...    60   8e-08
tr|B0NEY0|B0NEY0_EUBSP Putative uncharacterized protein OS=Clost...    54   4e-07
tr|A2PD01|A2PD01_VIBCH Putative uncharacterized protein OS=Vibri...    57   5e-07
tr|A8URE2|A8URE2_9AQUI Putative uncharacterized protein OS=Hydro...    55   3e-06
tr|Q3BKI1|Q3BKI1_9PROT Putative uncharacterized protein OS=Magne...    53   1e-05
tr|A4ECR2|A4ECR2_9ACTN Putative uncharacterized protein OS=Colli...    53   1e-05
tr|A9PE47|A9PE47_POPTR Putative uncharacterized protein OS=Popul...    53   1e-05
tr|B4VCZ2|B4VCZ2_9ACTO Putative uncharacterized protein OS=Strep...    53   1e-05
tr|B1BE08|B1BE08_CLOBO Putative uncharacterized protein OS=Clost...    51   3e-05
tr|B7R8W0|B7R8W0_9THEO Putative uncharacterized protein OS=Carbo...    47   5e-04
tr|A7PZE8|A7PZE8_VITVI Chromosome chr15 scaffold_40, whole genom...    46   0.001
tr|A6P1U3|A6P1U3_9BACE Putative uncharacterized protein OS=Bacte...    44   0.006

>tr|Q5M9Z9|Q5M9Z9_TOBAC Putative uncharacterized protein orf106b
           OS=Nicotiana tabacum GN=orf106b PE=4 SV=1
          Length = 106

 Score = 77.4 bits (189), Expect(3) = 1e-33
 Identities = 35/37 (94%), Positives = 36/37 (97%)
 Frame = -3

Query: 334 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSV 224
           VNCCASTHFGENQLALG+SGISPLTTTHPLI QHQSV
Sbjct: 48  VNCCASTHFGENQLALGASGISPLTTTHPLILQHQSV 84



 Score = 58.5 bits (140), Expect(3) = 1e-33
 Identities = 30/47 (63%), Positives = 34/47 (72%)
 Frame = -2

Query: 482 VKHIPHRLTG*PLSFVPFT*TLHLVFRVCLDLVPLSQPAPERCFTPR 342
           +K IPHRLT  P    P     +LVFRVCLDLVPLS+PAP+ CFTPR
Sbjct: 1   MKLIPHRLTSRPCP--PLILRSYLVFRVCLDLVPLSRPAPKPCFTPR 45



 Score = 52.0 bits (123), Expect(3) = 1e-33
 Identities = 21/21 (100%), Positives = 21/21 (100%)
 Frame = -1

Query: 222 GPPLSFTQASSWSWIDHPGSG 160
           GPPLSFTQASSWSWIDHPGSG
Sbjct: 85  GPPLSFTQASSWSWIDHPGSG 105


>tr|A6N1H4|A6N1H4_ORYSI Retrotransposon protein OS=Oryza sativa
           subsp. indica PE=4 SV=1
          Length = 109

 Score =  102 bits (253), Expect = 2e-20
 Identities = 58/96 (60%), Positives = 63/96 (65%), Gaps = 2/96 (2%)
 Frame = +3

Query: 273 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRE--RYQIEANSEY*M*RSSKRNEG* 446
           MPLEPRASWFSPKCVEAQQLT     K  F  G     + +   N+ Y       +  G 
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGCASGTKSRQTLNTRY-----DPKITGV 55

Query: 447 GSTSETMRDMLHRREGNSPDHQLRPLNDRSVVKEVG 554
            S SETM D LHRREGNSPDHQLRPLNDRSV+KEVG
Sbjct: 56  RSASETMGDKLHRREGNSPDHQLRPLNDRSVIKEVG 91


>tr|A7QCF1|A7QCF1_VITVI Chromosome undetermined scaffold_77, whole
           genome shotgun sequence OS=Vitis vinifera
           GN=GSVIVT00035369001 PE=4 SV=1
          Length = 235

 Score = 77.0 bits (188), Expect(2) = 1e-19
 Identities = 37/41 (90%), Positives = 38/41 (92%)
 Frame = -3

Query: 334 VNCCASTHFGENQLALGSSGISPLTTTHPLIFQHQSVRTST 212
           VN CASTHFGENQLALGSS ISPLTTTHPLI QHQSV+TST
Sbjct: 57  VNRCASTHFGENQLALGSSDISPLTTTHPLILQHQSVQTST 97



 Score = 43.5 bits (101), Expect(2) = 1e-19
 Identities = 18/23 (78%), Positives = 22/23 (95%)
 Frame = -2

Query: 410 VFRVCLDLVPLSQPAPERCFTPR 342
           +FRVCLDLVPLS+PA ++CFTPR
Sbjct: 32  MFRVCLDLVPLSRPALKQCFTPR 54


>tr|B7FGU0|B7FGU0_MEDTR Putative uncharacterized protein OS=Medicago
           truncatula PE=4 SV=1
          Length = 102

 Score = 96.3 bits (238), Expect = 1e-18
 Identities = 55/94 (58%), Positives = 62/94 (65%)
 Frame = +3

Query: 273 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY*M*RSSKRNEG*GS 452
           MPLEPRASWFSPKCVEAQQLT     K  F  G       E+ ++     +++      S
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGHLGVKHCFGAGR------ESGTKSRQTLNTRYPWRVKS 54

Query: 453 TSETMRDMLHRREGNSPDHQLRPLNDRSVVKEVG 554
            SE M D LHRREGNSPDHQLRPLNDRSV+KEVG
Sbjct: 55  ASEAMGDKLHRREGNSPDHQLRPLNDRSVIKEVG 88


>tr|Q85WT3|Q85WT3_PINKO ORF46f OS=Pinus koraiensis PE=4 SV=1
          Length = 46

 Score = 87.4 bits (215), Expect = 5e-16
 Identities = 42/46 (91%), Positives = 42/46 (91%)
 Frame = +3

Query: 273 MPLEPRASWFSPKCVEAQQLTRPSRGKAPFRCGLRERYQIEANSEY 410
           MPLEPRASWFSPKCVEAQQLT PSRGKA FR G RERYQIEANSEY
Sbjct: 1   MPLEPRASWFSPKCVEAQQLTGPSRGKALFRYGPRERYQIEANSEY 46


>tr|A5ZTV7|A5ZTV7_9FIRM Putative uncharacterized protein
           OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_02439 PE=4
           SV=1
          Length = 199

 Score = 79.3 bits (194), Expect = 1e-13
 Identities = 43/80 (53%), Positives = 52/80 (65%)
 Frame = -1

Query: 348 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 169
           P  + + A+P+ IS RTS L VR+ F P P LI   FN   FGPPL  T AS+W+WIDHP
Sbjct: 11  PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 168 GSGL*AVTIAPARLAFATAP 109
            SGL  +T+A  RL F TAP
Sbjct: 71  VSGLPPLTLALLRLGFPTAP 90


>tr|A5ZQM1|A5ZQM1_9FIRM Putative uncharacterized protein
           OS=Ruminococcus obeum ATCC 29174 GN=RUMOBE_01293 PE=4
           SV=1
          Length = 199

 Score = 79.3 bits (194), Expect = 1e-13
 Identities = 43/80 (53%), Positives = 52/80 (65%)
 Frame = -1

Query: 348 P*MV*STAAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHP 169
           P  + + A+P+ IS RTS L VR+ F P P LI   FN   FGPPL  T AS+W+WIDHP
Sbjct: 11  PPQISTKASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPLTAASTWTWIDHP 70

Query: 168 GSGL*AVTIAPARLAFATAP 109
            SGL  +T+A  RL F TAP
Sbjct: 71  VSGLPPLTLALLRLGFPTAP 90


>tr|A5KL06|A5KL06_9FIRM Putative uncharacterized protein
           OS=Ruminococcus torques ATCC 27756 GN=RUMTOR_00910 PE=4
           SV=1
          Length = 245

 Score = 76.6 bits (187), Expect = 8e-13
 Identities = 41/73 (56%), Positives = 48/73 (65%)
 Frame = -1

Query: 327 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 148
           A+P+ IS RTS L VR+ F P P LI   FN   FGPPL FT  S+W+WIDHP SGL  +
Sbjct: 4   ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 147 TIAPARLAFATAP 109
           T A  RL F +AP
Sbjct: 64  TFALFRLGFPSAP 76


>tr|A7B8Q0|A7B8Q0_RUMGN Putative uncharacterized protein
           OS=Ruminococcus gnavus ATCC 29149 GN=RUMGNA_03973 PE=4
           SV=1
          Length = 81

 Score = 71.6 bits (174), Expect(2) = 3e-12
 Identities = 37/64 (57%), Positives = 44/64 (68%)
 Frame = -1

Query: 327 AAPQRISGRTS*LWVRVAFHP*PQLIR*FFNISRFGPPLSFTQASSWSWIDHPGSGL*AV 148
           A+P+ IS RTS L VR+ F P P LI   FN   FGPPL FT  S+W+WIDHP SGL  +
Sbjct: 4   ASPKAISRRTSYLRVRLEFLPYPHLIPTLFNGCGFGPPLPFTATSTWTWIDHPVSGLLRL 63

Query: 147 TIAP 136
           TI+P
Sbjct: 64  TISP 67



 Score = 23.9 bits (50), Expect(2) = 3e-12
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = -2

Query: 140 PQQDSLSLRLRGFSP 96
           P  DS+SLRL+ FSP
Sbjct: 67  PCSDSVSLRLQAFSP 81


>tr|B1BC32|B1BC32_CLOBO Putative uncharacterized protein
           OS=Clostridium botulinum C str. Eklund GN=CBC_0457 PE=4
           SV=1
          Length = 93

 Score = 45.4 bits (106), Expect(2) = 4e-10
 Identities = 22/49 (44%), Positives = 29/49 (59%)
 Frame = -2

Query: 380 LSQPAPERCFTPRWSSQLLRLNAFRGEPASSGFEWHFTPNHNSSADFST 234
           +S P+P     P  +   L L  FRGEPA S F+W+F+  H+SS  FST
Sbjct: 1   MSAPSPFSALPPLLNYPTLALKLFRGEPAISEFDWNFSAIHSSSHGFST 49



 Score = 42.7 bits (99), Expect(2) = 4e-10
 Identities = 19/35 (54%), Positives = 21/35 (60%)
 Frame = -1

Query: 225 FGPPLSFTQASSWSWIDHPGSGL*AVTIAPARLAF 121
           FGPP +FT ASSW WI H  SGL   T  P +  F
Sbjct: 53  FGPPRNFTSASSWPWIGHSVSGLQYATSCPVQTRF 87