DK956814
Clone id TST39A01NGRL0026_L16
Library
Length 594
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_L16. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GCCAACAGCTAGGGATAACGAGGCGAAGGAATACAAAAGCTACAGCGACGGGCTTCACAT
CGGCTCTCTTCTCACCAACACCCCATCCACCATTTTTTGTGGCCGTGCGCGAGCTTGATC
TTCTCCCCTCGCCTGCAGCACTCCGTTTCAAAAAAATACACAAGCATAGATACTTTGCTA
CACGCACATTCGCGAAGTGTCCAATCTAGCGACTCCTCCCATCGGCTTCCTTCCTTCCCT
CGCTTGAAGAGCTCAGCGTGCTGATTGCAAGAGTTAGGTTTCTGTGCGAGAGCTAGGGTT
GGATCTCTAGATTCCGTTTCCCCATCTCGGTTGTCATGGCGATGGCACTTGCAGCTCTTC
GCCGTTTGCATCCTGCTGCCCGTTACGTAGCATGGAATGCTCTTTCACAAACATCTCGTT
CCTCGTCAGCCCTGGCAAATGAAGATTCCTACTCTCGTTTTGAAAGTTCGAAGGACAAGT
CCCATGTGACATGGCCAAAGCAGCTGAATGCTTCCCTTGAAGAGATCGACCCCGATATTA
CGGACATCATCCAACATGATAAAAACCGGCGGGTGCAACGGTTTGGAGTTGATT
■■Homology search results ■■ -
sp_hit_id Q9SZJ5
Definition sp|Q9SZJ5|GLYM_ARATH Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana
Align length 34
Score (bit) 56.2
E-value 1.0e-07
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956814|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_L16, 5'
(594 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q9SZJ5|GLYM_ARATH Serine hydroxymethyltransferase, mitochondr... 56 1e-07
sp|P50433|GLYM_SOLTU Serine hydroxymethyltransferase, mitochondr... 54 5e-07
sp|P49357|GLYM_FLAPR Serine hydroxymethyltransferase 1, mitochon... 48 3e-05
sp|P34899|GLYM_PEA Serine hydroxymethyltransferase, mitochondria... 47 9e-05
sp|P49358|GLYN_FLAPR Serine hydroxymethyltransferase 2, mitochon... 45 2e-04
sp|Q729U4|THYX_DESVH Thymidylate synthase thyX OS=Desulfovibrio ... 33 0.82
sp|Q8BSE0|RMD2_MOUSE Regulator of microtubule dynamics protein 2... 31 5.3
sp|O49420|FBK86_ARATH Putative F-box/Kelch-repeat protein At4g19... 31 5.3
sp|Q5R5W9|CE022_PONAB UPF0489 protein C5orf22 homolog OS=Pongo a... 30 9.1

>sp|Q9SZJ5|GLYM_ARATH Serine hydroxymethyltransferase, mitochondrial
OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1
Length = 517

Score = 56.2 bits (134), Expect = 1e-07
Identities = 23/34 (67%), Positives = 30/34 (88%)
Frame = +3

Query: 471 KDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
K++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 40 KERSRVTWPKQLNAPLEEVDPEIADIIEHEKARQ 73


>sp|P50433|GLYM_SOLTU Serine hydroxymethyltransferase, mitochondrial
OS=Solanum tuberosum PE=2 SV=1
Length = 518

Score = 54.3 bits (129), Expect = 5e-07
Identities = 27/49 (55%), Positives = 34/49 (69%)
Frame = +3

Query: 426 SALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
S+L NE Y K+KS V WPKQLNA LE +DP+I DII+H+K R+
Sbjct: 31 SSLPNEAVYD-----KEKSGVAWPKQLNAPLEVVDPEIADIIEHEKARQ 74


>sp|P49357|GLYM_FLAPR Serine hydroxymethyltransferase 1,
mitochondrial OS=Flaveria pringlei PE=2 SV=1
Length = 517

Score = 48.1 bits (113), Expect = 3e-05
Identities = 25/51 (49%), Positives = 34/51 (66%)
Frame = +3

Query: 420 SSSALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
S S+L +E Y K++ VTWPKQLNA LE +DP+I DII+ +K R+
Sbjct: 29 SMSSLPSEAVYE-----KERPGVTWPKQLNAPLEVVDPEIADIIELEKARQ 74


>sp|P34899|GLYM_PEA Serine hydroxymethyltransferase, mitochondrial
OS=Pisum sativum PE=1 SV=1
Length = 518

Score = 46.6 bits (109), Expect = 9e-05
Identities = 27/73 (36%), Positives = 38/73 (52%), Gaps = 15/73 (20%)
Frame = +3

Query: 399 ALSQTSRSSSALANEDSYSRFESS---------------KDKSHVTWPKQLNASLEEIDP 533
A++ R S+ N+ S F +S K+ VTWPKQLN+ LE IDP
Sbjct: 2 AMAMALRKLSSSVNKSSRPLFSASSLYYKSSLPDEAVYDKENPRVTWPKQLNSPLEVIDP 61

Query: 534 DITDIIQHDKNRR 572
+I DII+ +K R+
Sbjct: 62 EIADIIELEKARQ 74


>sp|P49358|GLYN_FLAPR Serine hydroxymethyltransferase 2,
mitochondrial OS=Flaveria pringlei PE=2 SV=1
Length = 517

Score = 45.4 bits (106), Expect = 2e-04
Identities = 25/51 (49%), Positives = 33/51 (64%)
Frame = +3

Query: 420 SSSALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
S S+L +E Y K++ VTWPKQLNA LE DP+I DII+ +K R+
Sbjct: 29 SMSSLPSEAVYE-----KERPGVTWPKQLNAPLEVGDPEIADIIELEKARQ 74


>sp|Q729U4|THYX_DESVH Thymidylate synthase thyX OS=Desulfovibrio
vulgaris (strain Hildenborough / ATCC 29579 / NCIMB
8303) GN=thyX PE=3 SV=1
Length = 245

Score = 33.5 bits (75), Expect = 0.82
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Frame = -2

Query: 257 AELFKRGKEGSRWEESLDWTLRECACSKVSM------LVYFFETECCRRGE 123
A L + G+ GSR E + L + A SK+ + LV+FFE CC R +
Sbjct: 142 ALLEQDGRTGSRANEDARFVLPQAAASKIVVTMNCRALVHFFEERCCMRAQ 192


>sp|Q8BSE0|RMD2_MOUSE Regulator of microtubule dynamics protein 2
OS=Mus musculus GN=Fam82a1 PE=2 SV=1
Length = 410

Score = 30.8 bits (68), Expect = 5.3
Identities = 16/64 (25%), Positives = 33/64 (51%), Gaps = 4/64 (6%)
Frame = +3

Query: 405 SQTSRSSSALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDI----TDIIQHDKNRR 572
S TS SS +E Y + ++ + +PK LN +EE+ D+ D ++ +++ +
Sbjct: 137 SATSNSSEEAESEGGYITANTDTEEQNFPFPKALNTHIEELKLDVLLQKADHLRTNESHK 196

Query: 573 VQRF 584
++ F
Sbjct: 197 MESF 200


>sp|O49420|FBK86_ARATH Putative F-box/Kelch-repeat protein At4g19930
OS=Arabidopsis thaliana GN=At4g19930 PE=1 SV=1
Length = 431

Score = 30.8 bits (68), Expect = 5.3
Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
Frame = +3

Query: 75 PTPHPPFFVAVRELDLLPSPAALRFKKIHK--HRYFATRTFAKCPI*RLLPSASFLPSL 245
P P+ PF + + L LP+ + +RFK + K +RTF RLL SF+ L
Sbjct: 39 PMPYIPFDLVIEILTRLPAKSLMRFKSVSKLWSSLICSRTFTN----RLLRVPSFIQRL 93


>sp|Q5R5W9|CE022_PONAB UPF0489 protein C5orf22 homolog OS=Pongo
abelii PE=2 SV=1
Length = 442

Score = 30.0 bits (66), Expect = 9.1
Identities = 14/57 (24%), Positives = 25/57 (43%)
Frame = -2

Query: 347 CHRHDNRDGETESRDPTLALAQKPNSCNQHAELFKRGKEGSRWEESLDWTLRECACS 177
C+ + D + ++ P LAL N+ + + + G E + D T E +CS
Sbjct: 165 CNNQEENDAVSSAKKPKLALEDSRNTASTNCDSSSEGLEKDTATQRSDQTCLEASCS 221


tr_hit_id Q8W4V3
Definition tr|Q8W4V3|Q8W4V3_CHLRE Serine hydroxymethyltransferase OS=Chlamydomonas reinhardtii
Align length 48
Score (bit) 67.0
E-value 8.0e-10
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956814|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_L16, 5'
(594 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|Q8W4V3|Q8W4V3_CHLRE Serine hydroxymethyltransferase OS=Chlamy... 67 8e-10
tr|A9NUX0|A9NUX0_PICSI Serine hydroxymethyltransferase OS=Picea ... 65 4e-09
tr|Q42289|Q42289_ARATH Glycine hydroxymethyltransferase (Fragmen... 56 1e-06
tr|Q7Y1F0|Q7Y1F0_ORYSJ Serine hydroxymethyltransferase OS=Oryza ... 55 4e-06
tr|Q6TUC6|Q6TUC6_ORYSJ Serine hydroxymethyltransferase (Fragment... 55 4e-06
tr|Q10D68|Q10D68_ORYSJ Serine hydroxymethyltransferase OS=Oryza ... 55 4e-06
tr|B8AJX7|B8AJX7_ORYSI Putative uncharacterized protein OS=Oryza... 55 4e-06
tr|A6XMY5|A6XMY5_TRIMO Serine hydroxymethyltransferase OS=Tritic... 53 1e-05
tr|B7FLU1|B7FLU1_MEDTR Putative uncharacterized protein OS=Medic... 51 4e-05
tr|B6T7Q7|B6T7Q7_MAIZE Serine hydroxymethyltransferase OS=Zea ma... 51 4e-05
tr|A9YWS0|A9YWS0_MEDTR Serine-hydroxymethyltransferase OS=Medica... 51 4e-05
tr|A7R0L6|A7R0L6_VITVI Chromosome undetermined scaffold_311, who... 51 6e-05
tr|A9SHC0|A9SHC0_PHYPA Serine hydroxymethyltransferase OS=Physco... 50 7e-05
tr|A9RQ31|A9RQ31_PHYPA Serine hydroxymethyltransferase OS=Physco... 49 3e-04
tr|A9PL10|A9PL10_POPTM Serine hydroxymethyltransferase OS=Populu... 48 5e-04
tr|A9PIN8|A9PIN8_POPJC Serine hydroxymethyltransferase OS=Populu... 48 5e-04
tr|A7R5N0|A7R5N0_VITVI Chromosome undetermined scaffold_1008, wh... 48 5e-04
tr|A3AMI0|A3AMI0_ORYSJ Serine hydroxymethyltransferase OS=Oryza ... 48 5e-04
tr|A9PL05|A9PL05_POPTM Serine hydroxymethyltransferase OS=Populu... 47 6e-04
tr|A9PJ09|A9PJ09_POPJC Serine hydroxymethyltransferase OS=Populu... 47 6e-04
tr|A7R5Q2|A7R5Q2_VITVI Chromosome undetermined scaffold_1022, wh... 47 6e-04
tr|A7R0L7|A7R0L7_VITVI Chromosome undetermined scaffold_311, who... 47 6e-04
tr|A5BJY0|A5BJY0_VITVI Putative uncharacterized protein OS=Vitis... 47 6e-04
tr|Q94C74|Q94C74_ARATH Serine hydroxymethyltransferase OS=Arabid... 47 8e-04
tr|Q8GRI1|Q8GRI1_ARATH Serine hydroxymethyltransferase OS=Arabid... 47 8e-04
tr|Q3E923|Q3E923_ARATH Serine hydroxymethyltransferase OS=Arabid... 47 8e-04
tr|A7NUI3|A7NUI3_VITVI Serine hydroxymethyltransferase OS=Vitis ... 47 8e-04
tr|A9PL06|A9PL06_POPTM Serine hydroxymethyltransferase OS=Populu... 44 0.005
tr|A4SBB9|A4SBB9_OSTLU Serine hydroxymethyltransferase OS=Ostreo... 44 0.007
tr|Q00VT2|Q00VT2_OSTTA Serine hydroxymethyltransferase OS=Ostreo... 39 0.22

>tr|Q8W4V3|Q8W4V3_CHLRE Serine hydroxymethyltransferase
OS=Chlamydomonas reinhardtii GN=shmt PE=2 SV=1
Length = 520

Score = 67.0 bits (162), Expect = 8e-10
Identities = 28/48 (58%), Positives = 37/48 (77%)
Frame = +3

Query: 429 ALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
AL+NE+ YSRF ++HVTWPK LNA L E+DPD+ DII+ +KNR+
Sbjct: 32 ALSNEEEYSRFSQDASRAHVTWPKVLNAGLAEVDPDLFDIIEKEKNRQ 79


>tr|A9NUX0|A9NUX0_PICSI Serine hydroxymethyltransferase OS=Picea
sitchensis PE=2 SV=1
Length = 519

Score = 64.7 bits (156), Expect = 4e-09
Identities = 30/56 (53%), Positives = 43/56 (76%)
Frame = +3

Query: 405 SQTSRSSSALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
S ++ + ++E +YSR E K+KSHVTWPKQLNA L E+DP+ITDI++ +KNR+
Sbjct: 21 SSVTQFAQRYSSEAAYSRTE--KEKSHVTWPKQLNAPLHEVDPEITDIVELEKNRQ 74


>tr|Q42289|Q42289_ARATH Glycine hydroxymethyltransferase (Fragment)
OS=Arabidopsis thaliana PE=2 SV=1
Length = 87

Score = 56.2 bits (134), Expect = 1e-06
Identities = 23/34 (67%), Positives = 30/34 (88%)
Frame = +3

Query: 471 KDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
K++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 36 KERSRVTWPKQLNAPLEEVDPEIADIIEHEKARQ 69


>tr|Q7Y1F0|Q7Y1F0_ORYSJ Serine hydroxymethyltransferase OS=Oryza
sativa subsp. japonica GN=OSJNBa0057G07.17 PE=3 SV=1
Length = 557

Score = 54.7 bits (130), Expect = 4e-06
Identities = 22/36 (61%), Positives = 32/36 (88%)
Frame = +3

Query: 465 SSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
+++++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 78 ATEERSGVTWPKQLNAPLEEVDPEIADIIEHEKARQ 113


>tr|Q6TUC6|Q6TUC6_ORYSJ Serine hydroxymethyltransferase (Fragment)
OS=Oryza sativa subsp. japonica PE=3 SV=1
Length = 434

Score = 54.7 bits (130), Expect = 4e-06
Identities = 22/36 (61%), Positives = 32/36 (88%)
Frame = +3

Query: 465 SSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
+++++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 78 ATEERSGVTWPKQLNAPLEEVDPEIADIIEHEKARQ 113


>tr|Q10D68|Q10D68_ORYSJ Serine hydroxymethyltransferase OS=Oryza
sativa subsp. japonica GN=Os03g0738400 PE=3 SV=1
Length = 513

Score = 54.7 bits (130), Expect = 4e-06
Identities = 22/36 (61%), Positives = 32/36 (88%)
Frame = +3

Query: 465 SSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
+++++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 34 ATEERSGVTWPKQLNAPLEEVDPEIADIIEHEKARQ 69


>tr|B8AJX7|B8AJX7_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_13440 PE=4 SV=1
Length = 513

Score = 54.7 bits (130), Expect = 4e-06
Identities = 22/36 (61%), Positives = 32/36 (88%)
Frame = +3

Query: 465 SSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
+++++S VTWPKQLNA LEE+DP+I DII+H+K R+
Sbjct: 34 ATEERSGVTWPKQLNAPLEEVDPEIADIIEHEKARQ 69


>tr|A6XMY5|A6XMY5_TRIMO Serine hydroxymethyltransferase OS=Triticum
monococcum PE=2 SV=1
Length = 510

Score = 52.8 bits (125), Expect = 1e-05
Identities = 27/61 (44%), Positives = 39/61 (63%)
Frame = +3

Query: 390 AWNALSQTSRSSSALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNR 569
A LS + S L S + +++++S VTWPKQLNA LEE+DP+I DII+ +K R
Sbjct: 6 ALRKLSARGQPLSRLTPLYSMASLPATEERSAVTWPKQLNAPLEEVDPEIADIIELEKAR 65

Query: 570 R 572
+
Sbjct: 66 Q 66


>tr|B7FLU1|B7FLU1_MEDTR Putative uncharacterized protein OS=Medicago
truncatula PE=2 SV=1
Length = 177

Score = 51.2 bits (121), Expect = 4e-05
Identities = 26/49 (53%), Positives = 35/49 (71%)
Frame = +3

Query: 426 SALANEDSYSRFESSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
S+L +E Y K+ S V+WPKQLN+SLEEIDP+I DII+ +K R+
Sbjct: 31 SSLPDEAVYD-----KENSRVSWPKQLNSSLEEIDPEIADIIELEKARQ 74


>tr|B6T7Q7|B6T7Q7_MAIZE Serine hydroxymethyltransferase OS=Zea mays
PE=2 SV=1
Length = 513

Score = 51.2 bits (121), Expect = 4e-05
Identities = 20/36 (55%), Positives = 31/36 (86%)
Frame = +3

Query: 465 SSKDKSHVTWPKQLNASLEEIDPDITDIIQHDKNRR 572
+++++S +TW KQLNA LEE+DP+I DII+H+K R+
Sbjct: 34 ATEERSGITWTKQLNAPLEEVDPEIADIIEHEKARQ 69