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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK956835
Clone id TST39A01NGRL0026_M13
Library
Length 517
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_M13. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
GTGTTGCATGATATAATGTTGTTTCGTAACGTTGCCACCAAGCCGTGTCCTTTCAGGAAC
ACATAATTTGATGAGACCTCATGCCCCATTATCATTATCACCTTTCATTTCATCGGATGT
GTTCCATCTACAGATCCACACTCGAAATCTGGATCTGTAGATTACACCACTCCGATTCAA
TGACAATACATTTTGCACTATGTCGTAGATGCTCTCCAATCATGTCGTCCCAAAGACCAT
GGACTGTCACCATCACGTTCCCACCACACTATACCTCCAGCACATCACCTTTTTCTATGA
AATTAGTATTATAGAAAATCATCATTTAGCTGTTTATTACATCAAAGAGGTCACTATCAT
TCAAAAGAAGACCAACATCTGCTCACGTACCGTAAGACTTTCCCTCTTTTCCCTACTCGA
CTTCTGGAGGGCTATTTCGCGTTTACTCCTGATTTTTATAAAAAGACAACACCTATATTT
CTGGCTGAAGTTTGAAAAGAGTATAGCAAAGGTGATT
■■Homology search results ■■ -
sp_hit_id O48962
Definition sp|O48962|EBP_ARATH Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana
Align length 56
Score (bit) 50.1
E-value 6.0e-06
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956835|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_M13, 5'
         (517 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O48962|EBP_ARATH Probable 3-beta-hydroxysteroid-Delta(8),Delt...    50   6e-06
sp|Q9FTZ2|EBP_ORYSJ Probable 3-beta-hydroxysteroid-Delta(8),Delt...    39   0.011
sp|Q9EQK5|MVP_MOUSE Major vault protein OS=Mus musculus GN=Mvp P...    32   2.3  
sp|Q05041|HNFAA_XENLA Hepatocyte nuclear factor 1-alpha-A OS=Xen...    30   5.1  
sp|Q6E0V4|CYB_PARGO Cytochrome b OS=Paraspadella gotoi GN=mt:Cyt...    30   5.1  
sp|Q5ZJK1|THOC5_CHICK THO complex subunit 5 homolog OS=Gallus ga...    30   6.8  
sp|Q65UX5|PYRR_MANSM Bifunctional protein pyrR OS=Mannheimia suc...    30   6.8  
sp|Q62667|MVP_RAT Major vault protein OS=Rattus norvegicus GN=Mv...    30   6.8  
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 OS=S...    30   8.8  
sp|Q9CLL7|PYRR_PASMU Bifunctional protein pyrR OS=Pasteurella mu...    30   8.8  

>sp|O48962|EBP_ARATH Probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           OS=Arabidopsis thaliana GN=At1g20050 PE=2 SV=1
          Length = 223

 Score = 50.1 bits (118), Expect = 6e-06
 Identities = 27/56 (48%), Positives = 36/56 (64%)
 Frame = +1

Query: 349 GHYHSKEDQHLLTYRKTFPLFPTRLLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           G   +K D+ L+ +  TF      +LEGYF F+P+F+K  T  +LAEV KEYSKGD
Sbjct: 48  GRKKAKLDKLLMCWW-TFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGD 102


>sp|Q9FTZ2|EBP_ORYSJ Probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           OS=Oryza sativa subsp. japonica GN=Os01g0103600 PE=2
           SV=1
          Length = 219

 Score = 39.3 bits (90), Expect = 0.011
 Identities = 16/32 (50%), Positives = 21/32 (65%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           ++EG F F P+F+    P +  EV KEYSKGD
Sbjct: 75  IIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGD 106


>sp|Q9EQK5|MVP_MOUSE Major vault protein OS=Mus musculus GN=Mvp PE=2
           SV=3
          Length = 861

 Score = 31.6 bits (70), Expect = 2.3
 Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = +1

Query: 268 TIPPAHHLFL*N*YYRKSSFSCLLHQRGHYHSKE-DQHLLTYRKTFPLFPTRLLE 429
           T+PP H+  + N   R +  S L    G    +  DQ +   +  FPL+P  LLE
Sbjct: 52  TVPPRHYCIVANPVSRDAQSSVLFDVTGQVRLRHADQEIRLAQDPFPLYPGELLE 106


>sp|Q05041|HNFAA_XENLA Hepatocyte nuclear factor 1-alpha-A
           OS=Xenopus laevis GN=hnf1-A PE=2 SV=1
          Length = 605

 Score = 30.4 bits (67), Expect = 5.1
 Identities = 17/44 (38%), Positives = 23/44 (52%)
 Frame = +3

Query: 159 RLHHSDSMTIHFALCRRCSPIMSSQRPWTVTITFPPHYTSSTSP 290
           +L + DS  +H  LC R SPI       TV+     HY +S+SP
Sbjct: 541 QLQNQDSSILHLPLCHRLSPIP------TVSSASLVHYHNSSSP 578


>sp|Q6E0V4|CYB_PARGO Cytochrome b OS=Paraspadella gotoi GN=mt:Cyt-b
           PE=3 SV=1
          Length = 378

 Score = 30.4 bits (67), Expect = 5.1
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +3

Query: 102 LSFHRMCSIYRSTLEIWICRLHHSDSMTIHFALCRRC 212
           LSF  +C I R   E WI RL H++  +  F +C  C
Sbjct: 63  LSFESVCHILRDVNEGWIIRLVHANGASFFF-ICLYC 98


>sp|Q5ZJK1|THOC5_CHICK THO complex subunit 5 homolog OS=Gallus
           gallus GN=THOC5 PE=2 SV=1
          Length = 698

 Score = 30.0 bits (66), Expect = 6.8
 Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
 Frame = +2

Query: 209 MLSNHVVPKTMDCHHHVPTTLYLQHITFFY--EISIIENHHLAVYYIKEVTIIQKKTNI 379
           ML  H +  T+D        L   H+TF+Y   ++I+   HL  YY+  + I+  KT +
Sbjct: 339 MLKRHPLSVTVDLKCKDENVL---HLTFYYLMNLNIMTVLHLTFYYLMNLNIMTVKTKV 394


>sp|Q65UX5|PYRR_MANSM Bifunctional protein pyrR OS=Mannheimia
           succiniciproducens (strain MBEL55E) GN=pyrR PE=3 SV=1
          Length = 179

 Score = 30.0 bits (66), Expect = 6.8
 Identities = 19/49 (38%), Positives = 26/49 (53%), Gaps = 7/49 (14%)
 Frame = +2

Query: 347 EVTIIQKKTNIC-------SRTVRLSLFSLLDFWRAISRLLLIFIKRQH 472
           E   +Q KT I         RT+R +L +L+DF RA    L+IF+ R H
Sbjct: 91  EFISVQNKTVILIDDVLFTGRTIRAALDALVDFGRAAKVELVIFVDRGH 139


>sp|Q62667|MVP_RAT Major vault protein OS=Rattus norvegicus GN=Mvp
           PE=1 SV=4
          Length = 861

 Score = 30.0 bits (66), Expect = 6.8
 Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = +1

Query: 268 TIPPAHHLFL*N*YYRKSSFSCLLHQRGHYHSKE-DQHLLTYRKTFPLFPTRLLE 429
           T+PP H+  + N   R +  S L    G    +  DQ +   +  FPL+P  +LE
Sbjct: 52  TVPPRHYCIVANPVSRDTQSSVLFDITGQVRLRHADQEIRLAQDPFPLYPGEVLE 106


>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
           OS=Saccharomyces cerevisiae GN=STE20 PE=1 SV=1
          Length = 939

 Score = 29.6 bits (65), Expect = 8.8
 Identities = 23/73 (31%), Positives = 35/73 (47%)
 Frame = +2

Query: 23  FVTLPPSRVLSGTHNLMRPHAPLSLSPFISSDVFHLQIHTRNLDL*ITPLRFNDNTFCTM 202
           F  +  S V+SG  + M PH+ +  +   S +     I+T N    ITP    DNTF T+
Sbjct: 94  FTRVSSSSVISGMSSSMSPHSNIDETK--SLEAVTPNINTSN----ITPDHSADNTFSTI 147

Query: 203 S*MLSNHVVPKTM 241
           +   S+H    T+
Sbjct: 148 NASESDHQFNDTL 160


>sp|Q9CLL7|PYRR_PASMU Bifunctional protein pyrR OS=Pasteurella
           multocida GN=pyrR PE=3 SV=1
          Length = 181

 Score = 29.6 bits (65), Expect = 8.8
 Identities = 14/29 (48%), Positives = 20/29 (68%)
 Frame = +2

Query: 386 RTVRLSLFSLLDFWRAISRLLLIFIKRQH 472
           RT+R +L +L+DF RA    L+IF+ R H
Sbjct: 111 RTIRAALDALVDFGRAAKIELVIFVDRGH 139
tr_hit_id A7XY75
Definition tr|A7XY75|A7XY75_SOLTU Putative C-8,7 sterol isomerase OS=Solanum tuberosum
Align length 32
Score (bit) 59.3
E-value 1.0e-07
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956835|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_M13, 5'
         (517 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A7XY75|A7XY75_SOLTU Putative C-8,7 sterol isomerase OS=Solanu...    59   1e-07
tr|A9P206|A9P206_PICSI Putative uncharacterized protein OS=Picea...    52   2e-05
tr|A9P0T7|A9P0T7_PICSI Putative uncharacterized protein OS=Picea...    52   2e-05
tr|A7NZR4|A7NZR4_VITVI Chromosome chr6 scaffold_3, whole genome ...    50   5e-05
tr|Q8LDC3|Q8LDC3_ARATH C-8,7 sterol isomerase OS=Arabidopsis tha...    50   7e-05
tr|Q0WRA8|Q0WRA8_ARATH C-8,7 sterol isomerase OS=Arabidopsis tha...    50   7e-05
tr|Q2TU57|Q2TU57_MAIZE Sterol-8,7-isomerase OS=Zea mays PE=2 SV=1      47   4e-04
tr|B6TIR9|B6TIR9_MAIZE 3-beta-hydroxysteroid-delta-isomerase OS=...    47   4e-04
tr|B6T8J7|B6T8J7_MAIZE 3-beta-hydroxysteroid-delta-isomerase OS=...    47   4e-04
tr|A3RD20|A3RD20_HORVU C-8,7-sterol isomerase OS=Hordeum vulgare...    46   0.001
tr|A9SKY9|A9SKY9_PHYPA Predicted protein OS=Physcomitrella paten...    44   0.004
tr|B8ACS4|B8ACS4_ORYSI Putative uncharacterized protein OS=Oryza...    39   0.12 
tr|Q1VU52|Q1VU52_9FLAO Putative uncharacterized protein OS=Psych...    35   1.7  
tr|A9TIX2|A9TIX2_PHYPA Predicted protein OS=Physcomitrella paten...    33   6.5  

>tr|A7XY75|A7XY75_SOLTU Putative C-8,7 sterol isomerase OS=Solanum
           tuberosum GN=SI1 PE=2 SV=1
          Length = 221

 Score = 59.3 bits (142), Expect = 1e-07
 Identities = 25/32 (78%), Positives = 30/32 (93%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           +LEGYF FTPDFY+KT+P++LAEV KEYSKGD
Sbjct: 75  VLEGYFVFTPDFYQKTSPVYLAEVWKEYSKGD 106


>tr|A9P206|A9P206_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 215

 Score = 52.0 bits (123), Expect = 2e-05
 Identities = 22/32 (68%), Positives = 27/32 (84%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           +LEGYF F+P+FYK  +P +LAEV KEYSKGD
Sbjct: 71  ILEGYFVFSPNFYKLNSPFYLAEVWKEYSKGD 102


>tr|A9P0T7|A9P0T7_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 214

 Score = 52.0 bits (123), Expect = 2e-05
 Identities = 22/32 (68%), Positives = 27/32 (84%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           +LEGYF F+P+FYK  +P +LAEV KEYSKGD
Sbjct: 71  ILEGYFVFSPNFYKLNSPFYLAEVWKEYSKGD 102


>tr|A7NZR4|A7NZR4_VITVI Chromosome chr6 scaffold_3, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00024612001
           PE=4 SV=1
          Length = 222

 Score = 50.4 bits (119), Expect = 5e-05
 Identities = 22/32 (68%), Positives = 26/32 (81%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           +LEGYF F+P+FYK  T  +LAEV KEYSKGD
Sbjct: 75  ILEGYFVFSPEFYKDKTACYLAEVWKEYSKGD 106


>tr|Q8LDC3|Q8LDC3_ARATH C-8,7 sterol isomerase OS=Arabidopsis
           thaliana PE=2 SV=1
          Length = 223

 Score = 50.1 bits (118), Expect = 7e-05
 Identities = 27/56 (48%), Positives = 36/56 (64%)
 Frame = +1

Query: 349 GHYHSKEDQHLLTYRKTFPLFPTRLLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           G   +K D+ L+ +  TF      +LEGYF F+P+F+K  T  +LAEV KEYSKGD
Sbjct: 48  GRKKAKLDKLLMCWW-TFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGD 102


>tr|Q0WRA8|Q0WRA8_ARATH C-8,7 sterol isomerase OS=Arabidopsis
           thaliana GN=At1g20050 PE=2 SV=1
          Length = 223

 Score = 50.1 bits (118), Expect = 7e-05
 Identities = 27/56 (48%), Positives = 36/56 (64%)
 Frame = +1

Query: 349 GHYHSKEDQHLLTYRKTFPLFPTRLLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           G   +K D+ L+ +  TF      +LEGYF F+P+F+K  T  +LAEV KEYSKGD
Sbjct: 48  GRKKAKLDKLLMCWW-TFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGD 102


>tr|Q2TU57|Q2TU57_MAIZE Sterol-8,7-isomerase OS=Zea mays PE=2 SV=1
          Length = 221

 Score = 47.4 bits (111), Expect = 4e-04
 Identities = 21/32 (65%), Positives = 24/32 (75%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           ++EG F FTPDF+KK  P F  EV KEYSKGD
Sbjct: 76  MIEGPFVFTPDFFKKENPNFFDEVWKEYSKGD 107


>tr|B6TIR9|B6TIR9_MAIZE 3-beta-hydroxysteroid-delta-isomerase OS=Zea
           mays PE=2 SV=1
          Length = 221

 Score = 47.4 bits (111), Expect = 4e-04
 Identities = 21/32 (65%), Positives = 24/32 (75%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           ++EG F FTPDF+KK  P F  EV KEYSKGD
Sbjct: 76  MIEGPFVFTPDFFKKENPNFFDEVWKEYSKGD 107


>tr|B6T8J7|B6T8J7_MAIZE 3-beta-hydroxysteroid-delta-isomerase OS=Zea
           mays PE=2 SV=1
          Length = 221

 Score = 47.4 bits (111), Expect = 4e-04
 Identities = 21/32 (65%), Positives = 24/32 (75%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           ++EG F FTPDF+KK  P F  EV KEYSKGD
Sbjct: 76  MIEGPFVFTPDFFKKENPNFFDEVWKEYSKGD 107


>tr|A3RD20|A3RD20_HORVU C-8,7-sterol isomerase OS=Hordeum vulgare
           PE=2 SV=1
          Length = 216

 Score = 45.8 bits (107), Expect = 0.001
 Identities = 21/32 (65%), Positives = 24/32 (75%)
 Frame = +1

Query: 421 LLEGYFAFTPDFYKKTTPIFLAEV*KEYSKGD 516
           L+EG F FTP F+ KT+P F  EV KEYSKGD
Sbjct: 71  LIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGD 102