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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK956858
Clone id TST39A01NGRL0026_N12
Library
Length 572
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_N12. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
AAGACTTCTATGTATAGTAGAGCCCTCACCATGGCAGCTTCATTCAGATACACAGCGCTG
CTCCTGGCCCTCATCTCTGCAACCGGCGCGCTTCTCCCACAGGAGGCCAATGCGACGGAC
GTGCAATTCGTGGTGGCGCAGCAGGTGGCGTTCGGGCAGGGCTTCGTCATCACGGGCAAT
ACTTCCAGCTTGGGCAACTGGACGTCCACTAACGACTCTCCGACATGTCTCTCGCTTCGG
GCTCCGAATCCTATTGGCTGCTCACTGTGGCATTGGATGCGGGGCTTTATGTGGAGTTCA
AGCCGGTGAGAGTTGTTTACGACACCAAAGAAGCGTTGGAATGGTATATAGGAAGCAACT
TGAACATCACAGTTCCTGTAAACAGCACGACAGTCTACATTCAGTGGAATGGTAGCTCCT
CGCTGACCGCCCAGCCAGCTATCGCGACTCTCTAGACATATCGAGCATGTTCACCATAAT
ATCAATTACATGCAAGATGAATGCCCACGCACGTTTTGCCCCCATCTAGGCCCTACTCAA
GCTTACCTTACCTTGCTTGGTATATGCTTCAT
■■Homology search results ■■ -
sp_hit_id P19584
Definition sp|P19584|AMYB_THETU Thermophilic beta-amylase OS=Thermoanaerobacter thermosulfurogenes
Align length 52
Score (bit) 33.5
E-value 0.015
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956858|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_N12, 5'
         (572 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P19584|AMYB_THETU Thermophilic beta-amylase OS=Thermoanaeroba...    33   0.015
sp|P26827|CDGT_THETU Cyclomaltodextrin glucanotransferase OS=The...    33   0.089
sp|P22998|AMY_STRVL Alpha-amylase OS=Streptomyces violaceus GN=a...    33   0.12 
sp|P31797|CDGT_BACST Cyclomaltodextrin glucanotransferase OS=Bac...    31   1.1  
sp|P23176|AMYG_ASPKA Glucoamylase I OS=Aspergillus kawachi GN=ga...    33   1.3  
sp|P0C6X1|R1AB_CVH22 Replicase polyprotein 1ab OS=Human coronavi...    32   1.7  
sp|P22832|AMYG_ASPSH Glucoamylase OS=Aspergillus shirousami GN=g...    32   2.2  
sp|Q06339|TFC6_YEAST Transcription factor tau 91 kDa subunit OS=...    31   5.0  

>sp|P19584|AMYB_THETU Thermophilic beta-amylase
           OS=Thermoanaerobacter thermosulfurogenes PE=1 SV=1
          Length = 551

 Score = 33.5 bits (75), Expect(2) = 0.015
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
 Frame = +3

Query: 255 WLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNST-TVYIQW 407
           W +T+ L  G  ++FK V++  D+   + W  GSN   TVP + T +V I W
Sbjct: 500 WTITLNLLPGEQIQFKAVKI--DSSGNVTWEGGSNHTYTVPTSGTGSVTITW 549



 Score = 25.0 bits (53), Expect(2) = 0.015
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 169 ITGNTSSLGNWTST 210
           I G+TS LGNW +T
Sbjct: 473 IVGSTSDLGNWNTT 486


>sp|P26827|CDGT_THETU Cyclomaltodextrin glucanotransferase
           OS=Thermoanaerobacter thermosulfurogenes GN=amyA PE=1
           SV=2
          Length = 710

 Score = 32.7 bits (73), Expect(2) = 0.089
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
 Frame = +3

Query: 255 WLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNST-TVYIQW 407
           W   V++ AG  ++FK ++   +T   + W  GSN   TVP +ST TV + W
Sbjct: 660 WYYDVSVPAGTTIQFKFIKKNGNT---ITWEGGSNHTYTVPSSSTGTVIVNW 708



 Score = 23.1 bits (48), Expect(2) = 0.089
 Identities = 7/14 (50%), Positives = 11/14 (78%)
 Frame = +1

Query: 169 ITGNTSSLGNWTST 210
           +TGN + LGNW ++
Sbjct: 630 LTGNVAELGNWDTS 643


>sp|P22998|AMY_STRVL Alpha-amylase OS=Streptomyces violaceus GN=aml
           PE=2 SV=1
          Length = 569

 Score = 32.7 bits (73), Expect(2) = 0.12
 Identities = 20/59 (33%), Positives = 27/59 (45%)
 Frame = +3

Query: 219 SDMSLASGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNSTTV 395
           S + L   +   W LTV L AG   E+K +R   D    + W  G+N   TVP +   V
Sbjct: 506 SALKLDPATYPVWKLTVGLPAGTSFEYKYIR--KDAAGNVTWESGANRTATVPASGQLV 562



 Score = 22.7 bits (47), Expect(2) = 0.12
 Identities = 7/11 (63%), Positives = 9/11 (81%)
 Frame = +1

Query: 169 ITGNTSSLGNW 201
           +TGN + LGNW
Sbjct: 492 VTGNRAELGNW 502


>sp|P31797|CDGT_BACST Cyclomaltodextrin glucanotransferase
           OS=Bacillus stearothermophilus GN=cgt PE=1 SV=1
          Length = 711

 Score = 31.2 bits (69), Expect(2) = 1.1
 Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
 Frame = +3

Query: 255 WLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNST-TVYIQW 407
           W + V++  G  +EFK ++   D++  + W  GSN   T P N+T  + + W
Sbjct: 660 WYIDVSVPEGKTIEFKFIKK--DSQGNVTWESGSNHVYTTPTNTTGKIIVDW 709



 Score = 20.8 bits (42), Expect(2) = 1.1
 Identities = 7/14 (50%), Positives = 9/14 (64%)
 Frame = +1

Query: 169 ITGNTSSLGNWTST 210
           I GN   LGNW ++
Sbjct: 630 IVGNVYELGNWDTS 643


>sp|P23176|AMYG_ASPKA Glucoamylase I OS=Aspergillus kawachi GN=gaI
           PE=1 SV=1
          Length = 639

 Score = 32.7 bits (73), Expect = 1.3
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = +3

Query: 237 SGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVP 377
           + S   W +TV L AG   E+K +RV  ++ +++EW    N   TVP
Sbjct: 580 TSSNPLWYVTVTLPAGESFEYKFIRV--ESDDSVEWESDPNREYTVP 624


>sp|P0C6X1|R1AB_CVH22 Replicase polyprotein 1ab OS=Human coronavirus
            229E GN=rep PE=1 SV=1
          Length = 6758

 Score = 32.3 bits (72), Expect = 1.7
 Identities = 23/86 (26%), Positives = 30/86 (34%), Gaps = 16/86 (18%)
 Frame = +2

Query: 191  WATGRPLTTLRHVSRFGLRILLAAHCGIGCGALC--GVQAGESCLRHQRSVGMVYRKQL- 361
            WA G  LTT+R+  + G       HC  G  A C   V     C +H      VY   + 
Sbjct: 5778 WAGGLELTTMRYFVKIG----AVKHCQCGTVATCYNSVSNDYCCFKHALGCDYVYNPYVI 5833

Query: 362  -------------EHHSSCKQHDSLH 400
                          HH+ C  H + H
Sbjct: 5834 DIQQWGYVGSLSTNHHAICNVHRNEH 5859


>sp|P22832|AMYG_ASPSH Glucoamylase OS=Aspergillus shirousami GN=glaA
           PE=3 SV=1
          Length = 639

 Score = 32.0 bits (71), Expect = 2.2
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = +3

Query: 237 SGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVP 377
           + S   W +TV L AG   E+K +RV  ++ +++EW    N   TVP
Sbjct: 580 TSSNPPWYVTVTLPAGESFEYKFIRV--ESDDSVEWESDPNREYTVP 624


>sp|Q06339|TFC6_YEAST Transcription factor tau 91 kDa subunit
           OS=Saccharomyces cerevisiae GN=TFC6 PE=1 SV=1
          Length = 672

 Score = 30.8 bits (68), Expect = 5.0
 Identities = 27/77 (35%), Positives = 38/77 (49%)
 Frame = +3

Query: 216 LSDMSLASGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNSTTV 395
           LS  +  S  E   + TVA+D   Y+ F P + +  TK  +  + GSNL   V       
Sbjct: 468 LSVSTAYSDFEDTVVSTVAVDGYFYI-FNP-KDIATTKTTVSRFRGSNLVPVVYCPQIYS 525

Query: 396 YIQWNGSSSLTAQPAIA 446
           YI  +G+SSL A P+ A
Sbjct: 526 YIYSDGASSLRAVPSRA 542
tr_hit_id A8J7T4
Definition tr|A8J7T4|A8J7T4_CHLRE Predicted protein OS=Chlamydomonas reinhardtii
Align length 60
Score (bit) 34.7
E-value 3.9
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK956858|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_N12, 5'
         (572 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A8J7T4|A8J7T4_CHLRE Predicted protein OS=Chlamydomonas reinha...    35   3.9  
tr|A8J4Z5|A8J4Z5_CHLRE Predicted protein OS=Chlamydomonas reinha...    35   3.9  
tr|B0B0V6|B0B0V6_STRGA Alpha-amylase GacE2 OS=Streptomyces glauc...    31   4.9  
tr|A1S2N2|A1S2N2_SHEAM Vibriolysin. Metallo peptidase. MEROPS fa...    34   5.0  
tr|Q53I75|Q53I75_HALME Putative alpha-amylase OS=Halobacterium m...    34   5.0  
tr|Q3MHX0|Q3MHX0_BOVIN Sulfite oxidase OS=Bos taurus GN=SUOX PE=...    34   6.6  
tr|B7SVK3|B7SVK3_9CORO Nonstructural protein 1b` (Fragment) OS=B...    33   8.6  

>tr|A8J7T4|A8J7T4_CHLRE Predicted protein OS=Chlamydomonas
           reinhardtii GN=CHLREDRAFT_192837 PE=4 SV=1
          Length = 296

 Score = 34.7 bits (78), Expect = 3.9
 Identities = 20/60 (33%), Positives = 31/60 (51%)
 Frame = +3

Query: 225 MSLASGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNSTTVYIQ 404
           M   SG +  W  TVAL AG  +E K + V   T   + W  G N+ +TVP +  ++ ++
Sbjct: 78  MKWTSGDQ--WTCTVALPAGSALECKYIVVDERTGREVRWQEGGNMLVTVPASVQSLPVE 135


>tr|A8J4Z5|A8J4Z5_CHLRE Predicted protein OS=Chlamydomonas
           reinhardtii GN=CHLREDRAFT_150073 PE=4 SV=1
          Length = 323

 Score = 34.7 bits (78), Expect = 3.9
 Identities = 22/58 (37%), Positives = 31/58 (53%)
 Frame = +3

Query: 186 QLGQLDVH*RLSDMSLASGSESYWLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSN 359
           +LG  D++  L  +S   G    W+ T+ L AG   E+K V V +D + AL W  GSN
Sbjct: 48  KLGNWDINKALV-LSWTDGDR--WVATIELPAGSVYEYKYVLVDHDGRSALAWQGGSN 102


>tr|B0B0V6|B0B0V6_STRGA Alpha-amylase GacE2 OS=Streptomyces
           glaucescens GN=gacE2 PE=3 SV=1
          Length = 569

 Score = 31.2 bits (69), Expect(2) = 4.9
 Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +3

Query: 255 WLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNSTTVYIQ-WNG 413
           W L VAL AG   E+K +R   D    + W  G N   TVP +  T+    W G
Sbjct: 518 WKLDVALPAGTSFEYKFLR--KDASGKVTWESGPNRTATVPSSGRTLLSDTWRG 569



 Score = 22.3 bits (46), Expect(2) = 4.9
 Identities = 7/11 (63%), Positives = 10/11 (90%)
 Frame = +1

Query: 169 ITGNTSSLGNW 201
           +TGN S+LG+W
Sbjct: 492 VTGNRSALGDW 502


>tr|A1S2N2|A1S2N2_SHEAM Vibriolysin. Metallo peptidase. MEROPS
           family M04 OS=Shewanella amazonensis (strain ATCC
           BAA-1098 / SB2B) GN=Sama_0428 PE=3 SV=1
          Length = 775

 Score = 34.3 bits (77), Expect = 5.0
 Identities = 28/95 (29%), Positives = 42/95 (44%), Gaps = 17/95 (17%)
 Frame = +3

Query: 216 LSDMSLASGSESYWLLTVAL--------------DAGLYVEF--KPVRVVYDTKEALEWY 347
           ++D+S ASGS+ YW L V                DA LYV+F   P    YD +    + 
Sbjct: 513 VTDISGASGSKQYWTLDVPAGASNLVFNLSGGSGDADLYVKFGANPTSTDYDCR---PYA 569

Query: 348 IGSNLNITVP-VNSTTVYIQWNGSSSLTAQPAIAT 449
            G+N N  +  V   T ++  NG SS +    + +
Sbjct: 570 AGNNENCAISNVQQGTYWVMLNGYSSYSGTTLVGS 604


>tr|Q53I75|Q53I75_HALME Putative alpha-amylase OS=Halobacterium
           mediterranei GN=amy PE=3 SV=1
          Length = 713

 Score = 34.3 bits (77), Expect = 5.0
 Identities = 19/48 (39%), Positives = 24/48 (50%)
 Frame = +3

Query: 255 WLLTVALDAGLYVEFKPVRVVYDTKEALEWYIGSNLNITVPVNSTTVY 398
           W   V L AG  +EFK V++  D    + W  GSN   T P +ST  Y
Sbjct: 663 WYYDVNLPAGTDIEFKFVKIASDG--TVTWESGSNRQYTTPTDSTGEY 708


>tr|Q3MHX0|Q3MHX0_BOVIN Sulfite oxidase OS=Bos taurus GN=SUOX PE=2
           SV=1
          Length = 547

 Score = 33.9 bits (76), Expect = 6.6
 Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
 Frame = -1

Query: 404 LNVDCRAVYRNCDVQVASYIPFQRFFGVVNNS-HRLELHIKP 282
           LN+ C+AV  + +VQ  +  P     GV+NN+ HR+ +H+ P
Sbjct: 506 LNIVCKAVDESYNVQPDTVAPIWNLRGVLNNAWHRVHVHVTP 547


>tr|B7SVK3|B7SVK3_9CORO Nonstructural protein 1b` (Fragment) OS=Bat
            coronavirus Trinidad/1CO7BA/2007 PE=4 SV=1
          Length = 1301

 Score = 33.5 bits (75), Expect = 8.6
 Identities = 23/88 (26%), Positives = 30/88 (34%), Gaps = 14/88 (15%)
 Frame = +2

Query: 179  ILPAWATGRPLTTLRHVSRFGLRILLAAHCGIGCGALCGVQAGESCLRHQRSVGMVYRK- 355
            I   WA G  LTT+R+  + G+  L   HCG         Q    C  H +    +Y   
Sbjct: 1094 IFVLWAGGLELTTMRYFVKVGVEKL--CHCGKVARCYASNQHQFYCFNHYQDCDYLYNPH 1151

Query: 356  -------------QLEHHSSCKQHDSLH 400
                          L HH  C  H + H
Sbjct: 1152 VIDIQQWGYQGSLSLNHHMHCNVHRNAH 1179