DK956916
Clone id TST39A01NGRL0026_P23
Library
Length 601
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_P23. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GCCTACTCTAATAATAATAAATCTTTGCTCTTGGAAGGCACTCTAGTTGCGCCCTTCTCT
TGCTCGCTTTCTGTTGCTTGCAAGAGAAGAGAATCTCTAAAAGCAGGCTTTTAAATTTTC
TACTTCTTCTGTGCATCTTCAGTTTTTATTTTTCCTTCTAGCTTTGTGTAATTAGGGGTG
ATTAGTGCCTAATTATCCATCAACAGCAAGCTTAACCACCCACCAACAAGCTTAATTACG
AGAAGATGGTTAATGACAGGACCCCGCTGCTGCCCCAAACCCAACAGGCAAGTGCTGGTA
ACTTTCCTTCAAGCAATGAAACAGGGAGGACCTCAAGCAGAAAAGGTGCGGTTTTGGTAG
CAGTTTGCGTGTTCCTCGGGCTTTTGGGCCTTTGGAGGTTGTCCTCCACAAACCCAGAAG
ACACAAAAACCATGCAGACTCTGTTGTCGGCAGCAGACCGGCCGCTGAGAGCCAACGAAG
GCCTCAATTATCTGGTGGTAGGCGATTGGGGAAGAGAGGGATTCTACAACCAGTCCCTGC
TCGCTACCCAAATGGGGAGGATTGCGCAAGAGCTGGGCATAGAATTTGTTATCTCGGCAG
G
■■Homology search results ■■ -
sp_hit_id Q74C57
Definition sp|Q74C57|APGM_GEOSL Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Geobacter sulfurreducens
Align length 57
Score (bit) 34.7
E-value 0.38
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956916|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_P23, 5'
(601 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q74C57|APGM_GEOSL Probable 2,3-bisphosphoglycerate-independen... 35 0.38
sp|Q80VY2|CA183_MOUSE Uncharacterized protein C1orf183 homolog O... 33 1.4
sp|Q5ND04|HSF5_MOUSE Heat shock factor protein 5 OS=Mus musculus... 32 3.2
sp|A4R596|IML1_MAGGR Vacuolar membrane-associated protein IML1 O... 31 4.2
sp|Q8WV44|TRI41_HUMAN Tripartite motif-containing protein 41 OS=... 31 5.5
sp|Q5RAB7|S39AE_PONAB Zinc transporter ZIP14 OS=Pongo abelii GN=... 31 5.5
sp|Q15043|S39AE_HUMAN Zinc transporter ZIP14 OS=Homo sapiens GN=... 31 5.5
sp|Q876L5|AKR1_SACBA Palmitoyltransferase AKR1 OS=Saccharomyces ... 30 7.1
sp|Q00975|CAC1B_HUMAN Voltage-dependent N-type calcium channel s... 30 7.2
sp|P58812|APGM_METAC 2,3-bisphosphoglycerate-independent phospho... 30 7.2
sp|Q14155|ARHG7_HUMAN Rho guanine nucleotide exchange factor 7 O... 30 9.3

>sp|Q74C57|APGM_GEOSL Probable 2,3-bisphosphoglycerate-independent
phosphoglycerate mutase OS=Geobacter sulfurreducens
GN=apgM PE=3 SV=1
Length = 399

Score = 34.7 bits (78), Expect = 0.38
Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +2

Query: 446 VGSRPAAESQRRPQLSGGRRLGKRGILQPVPARYPNGEDCARAG-----HRICYLGR 601
+G + ++ R P + R G+ G+ + VP YP G D A R CY GR
Sbjct: 19 LGGKTPLQAARTPHMDAMARRGRIGLARTVPEGYPPGSDVANLSVFGYDPRACYTGR 75


>sp|Q80VY2|CA183_MOUSE Uncharacterized protein C1orf183 homolog
OS=Mus musculus PE=2 SV=1
Length = 300

Score = 32.7 bits (73), Expect = 1.4
Identities = 27/73 (36%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
Frame = -3

Query: 413 VCGGQP---PKAQKPEEHANCYQNRTFSA*GPPCFIA*RKVTSTCLLGLGQQRGP-VINH 246
VCG + PK Q PEEH +C Q T ++ TST L+ G+ R P V+
Sbjct: 111 VCGKELAVLPKTQLPEEHQSCTQQGTE-------WVEPDDWTST-LMSRGRNRQPLVLGD 162

Query: 245 LLVIKLVGGWLSL 207
+ LVG WL L
Sbjct: 163 NVFADLVGNWLDL 175


>sp|Q5ND04|HSF5_MOUSE Heat shock factor protein 5 OS=Mus musculus
GN=Hsf5 PE=2 SV=1
Length = 624

Score = 31.6 bits (70), Expect = 3.2
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +3

Query: 255 DRTPLLPQTQQASAGNFPSSNETGRTSSRKG 347
DRTP+ P+T Q S G P ET T S KG
Sbjct: 250 DRTPIPPRTWQNSLGMHPGQVETSPTFSDKG 280


>sp|A4R596|IML1_MAGGR Vacuolar membrane-associated protein IML1
OS=Magnaporthe grisea GN=IML1 PE=3 SV=1
Length = 1846

Score = 31.2 bits (69), Expect = 4.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2

Query: 392 LEVVLHKPRRHKNHADSVVGSRPAAESQRRPQLSGGRRLGK 514
+++ KP R+ N+ DS A E + ++ G RRLGK
Sbjct: 1146 IDIATPKPERNWNYIDSTSLDPAAGEKEEETRIEGTRRLGK 1186


>sp|Q8WV44|TRI41_HUMAN Tripartite motif-containing protein 41
OS=Homo sapiens GN=TRIM41 PE=1 SV=3
Length = 630

Score = 30.8 bits (68), Expect = 5.5
Identities = 18/41 (43%), Positives = 24/41 (58%)
Frame = +3

Query: 423 TKTMQTLLSAADRPLRANEGLNYLVVGDWGREGFYNQSLLA 545
+ T QTLLS +++P R L+Y + GR GFYN LA
Sbjct: 563 SSTEQTLLSPSEKPRRFGVYLDY----EAGRLGFYNAETLA 599


>sp|Q5RAB7|S39AE_PONAB Zinc transporter ZIP14 OS=Pongo abelii
GN=SLC39A14 PE=2 SV=2
Length = 490

Score = 30.8 bits (68), Expect = 5.5
Identities = 27/85 (31%), Positives = 31/85 (36%), Gaps = 11/85 (12%)
Frame = -2

Query: 456 LLPTTESAWFLCLLGLWRTTSKGPKARGTRKLLPKPHLFCLRSSLFH-----------CL 310
L P +S L LLGLWRTT P+A + P L H L
Sbjct: 4 LHPAFQSCLLLTLLGLWRTT---PEAHASSPGAPAISAASFLQDLIHRYGEGDSLTLQQL 60

Query: 309 KESYQHLPVGFGAAAGSCH*PSSRN 235
K HL VG G + H RN
Sbjct: 61 KALLNHLDVGVGRGNVTQHVQGHRN 85


>sp|Q15043|S39AE_HUMAN Zinc transporter ZIP14 OS=Homo sapiens
GN=SLC39A14 PE=1 SV=2
Length = 492

Score = 30.8 bits (68), Expect = 5.5
Identities = 27/85 (31%), Positives = 31/85 (36%), Gaps = 11/85 (12%)
Frame = -2

Query: 456 LLPTTESAWFLCLLGLWRTTSKGPKARGTRKLLPKPHLFCLRSSLFH-----------CL 310
L P +S L LLGLWRTT P+A + P L H L
Sbjct: 6 LHPAFQSCLLLTLLGLWRTT---PEAHASSPGAPAISAASFLQDLIHRYGEGDSLTLQQL 62

Query: 309 KESYQHLPVGFGAAAGSCH*PSSRN 235
K HL VG G + H RN
Sbjct: 63 KALLNHLDVGVGRGNVTQHVQGHRN 87


>sp|Q876L5|AKR1_SACBA Palmitoyltransferase AKR1 OS=Saccharomyces
bayanus GN=AKR1 PE=3 SV=2
Length = 763

Score = 30.4 bits (67), Expect = 7.1
Identities = 23/62 (37%), Positives = 32/62 (51%)
Frame = +3

Query: 369 VFLGLLGLWRLSSTNPEDTKTMQTLLSAADRPLRANEGLNYLVVGDWGREGFYNQSLLAT 548
VFLGL S NP + LL+ A N+GLN LV+ +GR G +N +LL +
Sbjct: 331 VFLGL-AFALFSHVNPLFAIIVLFLLTLA-----TNKGLNKLVLPSYGRMGIHNVTLLRS 384

Query: 549 QM 554
+
Sbjct: 385 PL 386


>sp|Q00975|CAC1B_HUMAN Voltage-dependent N-type calcium channel
subunit alpha-1B OS=Homo sapiens GN=CACNA1B PE=1 SV=1
Length = 2339

Score = 30.4 bits (67), Expect = 7.2
Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2

Query: 410 KPRRHKNHADSVVGSRPA-AESQRRPQLSGGRRLGKRGILQPVPARYP 550
+PR H++H+ G A +E R P GGRR +RG + R P
Sbjct: 885 RPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREP 932


>sp|P58812|APGM_METAC 2,3-bisphosphoglycerate-independent
phosphoglycerate mutase OS=Methanosarcina acetivorans
GN=apgM PE=3 SV=1
Length = 397

Score = 30.4 bits (67), Expect = 7.2
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +2

Query: 446 VGSRPAAESQRRPQLSGGRRLGKRGILQPVPARYPNGEDCA 568
+GSR ++ R P + GK G+ + VP +P G D A
Sbjct: 19 LGSRTILQAARTPAMDSIAARGKTGLAKTVPEGFPPGSDVA 59



Score = 30.4 bits (67), Expect = 7.2
Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = -1

Query: 406 EDNLQRPKSPRNTQTATKTAPFLLEVLPVSLL---EGKLPALACWVWG 272
+D L R K + + +LE+ PV+L EGK PA + WVWG
Sbjct: 171 DDYLPRGKDGEFFSGLIEASTVVLELHPVNLKRVQEGKNPANSIWVWG 218


tr_hit_id A9NRL4
Definition tr|A9NRL4|A9NRL4_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 43
Score (bit) 65.9
E-value 2.0e-09
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK956916|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0026_P23, 5'
(601 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9NRL4|A9NRL4_PICSI Putative uncharacterized protein OS=Picea... 66 2e-09
tr|A9NZW8|A9NZW8_PICSI Putative uncharacterized protein OS=Picea... 65 2e-09
tr|Q71SA5|Q71SA5_LILLO Acid phosphatase (Fragment) OS=Lilium lon... 64 5e-09
tr|A9SJW3|A9SJW3_PHYPA Predicted protein (Fragment) OS=Physcomit... 64 9e-09
tr|A9NSG6|A9NSG6_PICSI Putative uncharacterized protein OS=Picea... 62 2e-08
tr|A7P405|A7P405_VITVI Chromosome chr1 scaffold_5, whole genome ... 62 3e-08
tr|A5BBI4|A5BBI4_VITVI Putative uncharacterized protein OS=Vitis... 62 3e-08
tr|Q9LL79|Q9LL79_PHAVU Putative purple acid phosphatase OS=Phase... 61 6e-08
tr|Q10PD0|Q10PD0_ORYSJ Os03g0238600 protein OS=Oryza sativa subs... 61 6e-08
tr|A2XEC8|A2XEC8_ORYSI Putative uncharacterized protein OS=Oryza... 61 6e-08
tr|B8A2D7|B8A2D7_MAIZE Putative uncharacterized protein OS=Zea m... 60 7e-08
tr|B6TWW2|B6TWW2_MAIZE Purple acid phosphatase 1 OS=Zea mays PE=... 60 7e-08
tr|B4FM74|B4FM74_MAIZE Putative uncharacterized protein OS=Zea m... 60 7e-08
tr|Q2R2T5|Q2R2T5_ORYSJ Ser/Thr protein phosphatase family protei... 59 3e-07
tr|A3CC79|A3CC79_ORYSJ Putative uncharacterized protein OS=Oryza... 59 3e-07
tr|A2ZF81|A2ZF81_ORYSI Putative uncharacterized protein OS=Oryza... 59 3e-07
tr|Q9LL80|Q9LL80_SOYBN Putative purple acid phosphatase OS=Glyci... 58 4e-07
tr|B8LPI1|B8LPI1_PICSI Putative uncharacterized protein OS=Picea... 58 4e-07
tr|B4FPR9|B4FPR9_MAIZE Putative uncharacterized protein OS=Zea m... 58 4e-07
tr|Q8W5L9|Q8W5L9_ORYSA Putative purple acid phosphatase OS=Oryza... 58 5e-07
tr|Q7XH73|Q7XH73_ORYSJ Os10g0116800 protein OS=Oryza sativa subs... 58 5e-07
tr|B6TE04|B6TE04_MAIZE Purple acid phosphatase 1 OS=Zea mays PE=... 57 6e-07
tr|B5KRH3|B5KRH3_BRANA Purple acid phosphatase 17 OS=Brassica na... 57 6e-07
tr|Q9LL81|Q9LL81_IPOBA Putative purple acid phosphatase OS=Ipomo... 57 8e-07
tr|Q8VYU7|Q8VYU7_ARATH At1g25230/F4F7_8 (Putative purple acid ph... 57 8e-07
tr|B5KRH1|B5KRH1_BRANA Purple acid phosphatase 17 OS=Brassica na... 57 8e-07
tr|A9P960|A9P960_POPTR Putative uncharacterized protein OS=Popul... 57 8e-07
tr|Q9SIS5|Q9SIS5_ARATH Putative purple acid phosphatase OS=Arabi... 57 1e-06
tr|Q9SDZ8|Q9SDZ8_ARATH Putative purple acid phosphatase OS=Arabi... 57 1e-06
tr|Q8VYZ2|Q8VYZ2_ARATH Putative purple acid phosphatase OS=Arabi... 57 1e-06

>tr|A9NRL4|A9NRL4_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 352

Score = 65.9 bits (159), Expect = 2e-09
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = +3

Query: 468 RANEGLNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
+ LN+LVVGDWGR+G+YNQS +ATQMGRI +EL I+FVIS
Sbjct: 48 KTGNSLNFLVVGDWGRKGYYNQSEVATQMGRIGEELDIDFVIS 90


>tr|A9NZW8|A9NZW8_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 350

Score = 65.5 bits (158), Expect = 2e-09
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = +3

Query: 468 RANEGLNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
+ + LN+LVVGDWGR+G YNQS +ATQMGRI +EL I+FVIS
Sbjct: 46 KIGDSLNFLVVGDWGRKGLYNQSRVATQMGRIGEELDIDFVIS 88


>tr|Q71SA5|Q71SA5_LILLO Acid phosphatase (Fragment) OS=Lilium
longiflorum PE=2 SV=1
Length = 111

Score = 64.3 bits (155), Expect = 5e-09
Identities = 28/51 (54%), Positives = 40/51 (78%)
Frame = +3

Query: 444 LSAADRPLRANEGLNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
L ++P++A+ L+ LV+GDWGR G YNQS +ATQMG+I +EL I+FV+S
Sbjct: 26 LPRLEQPIKADGSLSLLVIGDWGRNGGYNQSQVATQMGKIGEELDIDFVVS 76


>tr|A9SJW3|A9SJW3_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_131125 PE=4 SV=1
Length = 278

Score = 63.5 bits (153), Expect = 9e-09
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +3

Query: 483 LNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
LN+LVVGDWGREG YNQ+L+A QMGR+ LGI+FVIS
Sbjct: 1 LNFLVVGDWGREGAYNQTLVAEQMGRVGHHLGIDFVIS 38


>tr|A9NSG6|A9NSG6_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 349

Score = 62.4 bits (150), Expect = 2e-08
Identities = 27/43 (62%), Positives = 36/43 (83%)
Frame = +3

Query: 468 RANEGLNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
+A LN+LVVGDWGR+G YNQS +A QMGRIA++L ++F+IS
Sbjct: 33 QAGGSLNFLVVGDWGRKGLYNQSQVAYQMGRIAEDLDVDFIIS 75


>tr|A7P405|A7P405_VITVI Chromosome chr1 scaffold_5, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00030645001
PE=4 SV=1
Length = 236

Score = 61.6 bits (148), Expect = 3e-08
Identities = 33/76 (43%), Positives = 47/76 (61%)
Frame = +3

Query: 369 VFLGLLGLWRLSSTNPEDTKTMQTLLSAADRPLRANEGLNYLVVGDWGREGFYNQSLLAT 548
VF L+GL +SS L ++ +A+ L++LVVGDWGR G +NQS +A
Sbjct: 6 VFTALVGLCSVSSVAE---------LLRLEQQAKADGSLSFLVVGDWGRRGSFNQSRVAL 56

Query: 549 QMGRIAQELGIEFVIS 596
QMGR+ +EL I+FV+S
Sbjct: 57 QMGRVGEELDIDFVVS 72


>tr|A5BBI4|A5BBI4_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_002197 PE=4 SV=1
Length = 288

Score = 61.6 bits (148), Expect = 3e-08
Identities = 33/76 (43%), Positives = 47/76 (61%)
Frame = +3

Query: 369 VFLGLLGLWRLSSTNPEDTKTMQTLLSAADRPLRANEGLNYLVVGDWGREGFYNQSLLAT 548
VF L+GL +SS L ++ +A+ L++LVVGDWGR G +NQS +A
Sbjct: 6 VFTALVGLCSVSSVAE---------LLRLEQQAKADGSLSFLVVGDWGRRGSFNQSRVAL 56

Query: 549 QMGRIAQELGIEFVIS 596
QMGR+ +EL I+FV+S
Sbjct: 57 QMGRVGEELDIDFVVS 72


>tr|Q9LL79|Q9LL79_PHAVU Putative purple acid phosphatase
OS=Phaseolus vulgaris GN=PAP PE=2 SV=1
Length = 331

Score = 60.8 bits (146), Expect = 6e-08
Identities = 26/55 (47%), Positives = 40/55 (72%)
Frame = +3

Query: 432 MQTLLSAADRPLRANEGLNYLVVGDWGREGFYNQSLLATQMGRIAQELGIEFVIS 596
+ LL + P++A+ L+ +V+GDWGR+G YNQS ++ QMGR+ +L I+FVIS
Sbjct: 18 VSALLQRLEHPVKADGSLSLVVIGDWGRKGTYNQSEVSAQMGRVGAKLNIDFVIS 72


>tr|Q10PD0|Q10PD0_ORYSJ Os03g0238600 protein OS=Oryza sativa subsp.
japonica GN=Os03g0238600 PE=2 SV=1
Length = 339

Score = 60.8 bits (146), Expect = 6e-08
Identities = 35/90 (38%), Positives = 49/90 (54%)
Frame = +3

Query: 327 RTSSRKGAVLVAVCVFLGLLGLWRLSSTNPEDTKTMQTLLSAADRPLRANEGLNYLVVGD 506
R+SSR A + VF L S L + P +++ L+ LVVGD
Sbjct: 4 RSSSRACAAMATAAVFALLAATATASG------------LVRVEHPAKSDGSLSLLVVGD 51

Query: 507 WGREGFYNQSLLATQMGRIAQELGIEFVIS 596
WGR+G YNQS +A QMG++ ++L I+FVIS
Sbjct: 52 WGRKGTYNQSRVAEQMGKVGEKLNIDFVIS 81


>tr|A2XEC8|A2XEC8_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_10684 PE=4 SV=1
Length = 339

Score = 60.8 bits (146), Expect = 6e-08
Identities = 35/90 (38%), Positives = 49/90 (54%)
Frame = +3

Query: 327 RTSSRKGAVLVAVCVFLGLLGLWRLSSTNPEDTKTMQTLLSAADRPLRANEGLNYLVVGD 506
R+SSR A + VF L S L + P +++ L+ LVVGD
Sbjct: 4 RSSSRACAAMATAAVFALLAATATASG------------LVRVEHPAKSDGSLSLLVVGD 51

Query: 507 WGREGFYNQSLLATQMGRIAQELGIEFVIS 596
WGR+G YNQS +A QMG++ ++L I+FVIS
Sbjct: 52 WGRKGTYNQSRVAEQMGKVGEKLNIDFVIS 81