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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK957216
Clone id TST39A01NGRL0027_M13
Library
Length 623
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0027_M13. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
AACAAATTTCAGGGGCAAACCTCCCTAATAAATATCTTTGAGGGCGAGGGTTTGTGTATT
GTCTGTGTAGGGTTTAGGTTTGCTGACGAGAAGTATCGGCCATGAGTAACCAGCTCACGC
GCAGGCTGTCGAAACAGGCATCGTTACTCAGCAGGGCGCTGAAAGGAGGGGATGGATTGC
TGGGCAGATCGCAGCAATCAAGGTCGTTTATGTATCTTGCAGGGAGAGATGCCGCAGACC
GCAAGGCTGAGACGAAGGCTTACATGCGGCTCGGTGTAAGAGGCATAAGAAGAGAGCCCA
TCTCGCAAGCACAGCGCAATGCAGAGCTGGAAGAGGAGGCTAAAAGAATTGACCTTGCTC
CCCACAAGCTCCTCGAGAAGGGTGAGCCTGGCACCATGCAGGCCATCATCAAGGATGTGA
TGCCTGTGGGGTATTTTTTGACTCTCCCAACAGGAAGAGAAGGATATCTTCCCGCACTTG
ATCTTGGCTTTACAGGTGGCATTGTCATCCTCGAGCGGCTTTTCAAAGTGGGTCAGGAGT
TGACTGTGCGTGTGGTGCGGCGAGGAGGCGGTGGCCGTGAGATTCTTTCCATAAAGAAGC
CAGAGTTTGCGCCTAATACTGAT
■■Homology search results ■■ -
sp_hit_id Q46JM7
Definition sp|Q46JM7|PNP_PROMT Polyribonucleotide nucleotidyltransferase OS=Prochlorococcus marinus (strain NATL2A)
Align length 84
Score (bit) 31.2
E-value 4.5
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK957216|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0027_M13, 5'
         (623 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q46JM7|PNP_PROMT Polyribonucleotide nucleotidyltransferase OS...    31   4.5  
sp|A2C412|PNP_PROM1 Polyribonucleotide nucleotidyltransferase OS...    31   4.5  
sp|Q09856|YAF2_SCHPO Uncharacterized protein C29E6.02 OS=Schizos...    31   5.9  
sp|Q3ABT4|SYFA_CARHZ Phenylalanyl-tRNA synthetase alpha chain OS...    31   5.9  
sp|Q82F68|SYD_STRAW Aspartyl-tRNA synthetase OS=Streptomyces ave...    31   5.9  
sp|A7Z3X1|MTNK_BACA2 Methylthioribose kinase OS=Bacillus amyloli...    31   5.9  
sp|Q9SRL5|FRI2_ARATH Ferritin-2, chloroplastic OS=Arabidopsis th...    30   7.7  

>sp|Q46JM7|PNP_PROMT Polyribonucleotide nucleotidyltransferase
           OS=Prochlorococcus marinus (strain NATL2A) GN=pnp PE=3
           SV=1
          Length = 722

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 24/84 (28%), Positives = 43/84 (51%), Gaps = 2/84 (2%)
 Frame = +3

Query: 300 ISQAQRNAELEEEAKRIDLAPHKLLEKGE--PGTMQAIIKDVMPVGYFLTLPTGREGYLP 473
           ++ A  +    EEA+RI     + + +GE  PG++  II    P+G F+ +  G+EG + 
Sbjct: 604 VTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITRII----PIGAFVEILPGKEGMIH 659

Query: 474 ALDLGFTGGIVILERLFKVGQELT 545
              L     +  +E + KVG ++T
Sbjct: 660 ISQLS-EARVEKVEDVVKVGDQVT 682


>sp|A2C412|PNP_PROM1 Polyribonucleotide nucleotidyltransferase
           OS=Prochlorococcus marinus (strain NATL1A) GN=pnp PE=3
           SV=1
          Length = 722

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 24/84 (28%), Positives = 43/84 (51%), Gaps = 2/84 (2%)
 Frame = +3

Query: 300 ISQAQRNAELEEEAKRIDLAPHKLLEKGE--PGTMQAIIKDVMPVGYFLTLPTGREGYLP 473
           ++ A  +    EEA+RI     + + +GE  PG++  II    P+G F+ +  G+EG + 
Sbjct: 604 VTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITRII----PIGAFVEILPGKEGMIH 659

Query: 474 ALDLGFTGGIVILERLFKVGQELT 545
              L     +  +E + KVG ++T
Sbjct: 660 ISQLS-EARVEKVEDVVKVGDQVT 682


>sp|Q09856|YAF2_SCHPO Uncharacterized protein C29E6.02
           OS=Schizosaccharomyces pombe GN=SPAC29E6.02 PE=2 SV=1
          Length = 542

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 23/68 (33%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
 Frame = +3

Query: 225 RDAADRKAETKAYMRLGVRGIRREPISQAQRNAELEEEAKRIDLAPHKL-LEKGEPGTMQ 401
           R    RK      + L   G   E  +QA+R A LE+  KRI L  HK  +E     T +
Sbjct: 173 RTKESRKTRGSRNLHLNESGKFIEEANQARRQARLEDLKKRIALHSHKAGIEDELDITSK 232

Query: 402 AIIKDVMP 425
           +I +D +P
Sbjct: 233 SIGRDTIP 240


>sp|Q3ABT4|SYFA_CARHZ Phenylalanyl-tRNA synthetase alpha chain
           OS=Carboxydothermus hydrogenoformans (strain Z-2901 /
           DSM 6008) GN=pheS PE=3 SV=1
          Length = 340

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 21/65 (32%), Positives = 29/65 (44%)
 Frame = -1

Query: 401 LHGARLTLLEELVGSKVNSFSLLFQLCIALCLRDGLSSYASYTEPHVSLRLSLAVCGISP 222
           L G  LT ++EL G K             + LR    SY  +TEP   + +S  +CG   
Sbjct: 226 LKGVLLTFIKELFGPKTK-----------MRLRP---SYFPFTEPSAEVDISCVICGGIG 271

Query: 221 CKIHK 207
           CK+ K
Sbjct: 272 CKVCK 276


>sp|Q82F68|SYD_STRAW Aspartyl-tRNA synthetase OS=Streptomyces
           avermitilis GN=aspS PE=3 SV=1
          Length = 587

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 19/58 (32%), Positives = 30/58 (51%)
 Frame = +3

Query: 189 SQQSRSFMYLAGRDAADRKAETKAYMRLGVRGIRREPISQAQRNAELEEEAKRIDLAP 362
           S Q+R F    G  A ++ A+  A++R+G  G    PI++      + E  KR+ LAP
Sbjct: 328 SGQTRKFFDGLGDYAVEQGAKGLAWVRVGEDGSLTGPIAKFLTEENVAELTKRLSLAP 385


>sp|A7Z3X1|MTNK_BACA2 Methylthioribose kinase OS=Bacillus
           amyloliquefaciens (strain FZB42) GN=mtnK PE=3 SV=1
          Length = 394

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = -1

Query: 464 SFSSCWESQKIPHRHHILDDGLHGARLTLLEELVG 360
           +FS  WE   +P R H+L+D L  A     EE VG
Sbjct: 299 TFSEAWEKDCLPSRRHLLEDTLKNA----FEEAVG 329


>sp|Q9SRL5|FRI2_ARATH Ferritin-2, chloroplastic OS=Arabidopsis
           thaliana GN=FER2 PE=2 SV=1
          Length = 253

 Score = 30.4 bits (67), Expect = 7.7
 Identities = 22/91 (24%), Positives = 36/91 (39%)
 Frame = +3

Query: 114 LTRRLSKQASLLSRALKGGDGLLGRSQQSRSFMYLAGRDAADRKAETKAYMRLGVRGIRR 293
           +  + S   SLLS    GG  L   S+ S + ++         +A  K      + G+  
Sbjct: 1   MLHKASPALSLLSSGYTGGGNLFPPSRNSSNLLFSPSGSRFSVQA-AKGTNTKSLTGVVF 59

Query: 294 EPISQAQRNAELEEEAKRIDLAPHKLLEKGE 386
           EP  + ++  EL      + LA HK  +  E
Sbjct: 60  EPFEEVKKEMELVPTTPFVSLARHKFSDDSE 90
tr_hit_id A8F7S1
Definition tr|A8F7S1|A8F7S1_THELT RNA binding S1 domain protein OS=Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Align length 58
Score (bit) 37.4
E-value 0.73
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK957216|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0027_M13, 5'
         (623 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|A8F7S1|A8F7S1_THELT RNA binding S1 domain protein OS=Thermoto...    37   0.73 
tr|Q2RLT1|Q2RLT1_MOOTA Ribonuclease R OS=Moorella thermoacetica ...    35   4.7  
tr|P74405|P74405_SYNY3 Potassium channel OS=Synechocystis sp. (s...    35   4.7  
tr|B4SMT9|B4SMT9_STRM5 Glutathionylspermidine synthase OS=Stenot...    34   6.2  
tr|A6W4K0|A6W4K0_KINRD Seryl-tRNA synthetase OS=Kineococcus radi...    34   6.2  
tr|Q4K792|Q4K792_PSEF5 Transcriptional regulator, AraC family OS...    34   8.0  

>tr|A8F7S1|A8F7S1_THELT RNA binding S1 domain protein OS=Thermotoga
           lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
           GN=Tlet_1651 PE=4 SV=1
          Length = 539

 Score = 37.4 bits (85), Expect = 0.73
 Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
 Frame = +3

Query: 366 KLLEKGEPGTM-QAIIKDVMPVGYFLTLPTGREGYLPALDLGFTGGIVILERLFKVGQ 536
           K+ E+   GT+ Q  +K +MP G FL L  G +G +   ++ +T     L + FK+GQ
Sbjct: 266 KVSERYSVGTVVQGTVKSIMPFGIFLNLEPGLDGLIHISEVFWTSKKADLHKYFKIGQ 323


>tr|Q2RLT1|Q2RLT1_MOOTA Ribonuclease R OS=Moorella thermoacetica
           (strain ATCC 39073) GN=Moth_0273 PE=3 SV=1
          Length = 770

 Score = 34.7 bits (78), Expect = 4.7
 Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
 Frame = +3

Query: 231 AADRKAETKAYMRLGVRGIRREPISQAQRNAELEEEAKR--IDLAPHKLLEKGEPGTMQA 404
           +A+R A+  A++ L           QA    +L EEA+R  +D+   + +E+    T   
Sbjct: 576 SAERMAQLSAFVALAA--------VQASEREKLAEEAERESLDMKKVQYMERHVGATFAG 627

Query: 405 IIKDVMPVGYFLTLPTGREG 464
           +I  V+P G+F+ L    EG
Sbjct: 628 VISGVVPYGFFVELENTVEG 647


>tr|P74405|P74405_SYNY3 Potassium channel OS=Synechocystis sp.
           (strain PCC 6803) GN=sll0261 PE=4 SV=1
          Length = 568

 Score = 34.7 bits (78), Expect = 4.7
 Identities = 14/33 (42%), Positives = 22/33 (66%)
 Frame = -1

Query: 461 FSSCWESQKIPHRHHILDDGLHGARLTLLEELV 363
           FS   ++ K+P RHHI+  GL G  + ++EEL+
Sbjct: 324 FSQFLDAAKLPDRHHIIICGLGGVSMAIIEELI 356


>tr|B4SMT9|B4SMT9_STRM5 Glutathionylspermidine synthase
           OS=Stenotrophomonas maltophilia (strain R551-3)
           GN=Smal_3849 PE=4 SV=1
          Length = 392

 Score = 34.3 bits (77), Expect = 6.2
 Identities = 19/55 (34%), Positives = 29/55 (52%)
 Frame = -1

Query: 371 ELVGSKVNSFSLLFQLCIALCLRDGLSSYASYTEPHVSLRLSLAVCGISPCKIHK 207
           +LVG  + S  L+ QL I    RD ++      +PH+  RL LA  G  P K+++
Sbjct: 62  DLVGDVIGSERLMDQLAIPSHYRDWIADSWRQRQPHLYGRLDLAYDGTGPAKLYE 116


>tr|A6W4K0|A6W4K0_KINRD Seryl-tRNA synthetase OS=Kineococcus
           radiotolerans (strain ATCC BAA-149 / DSM 14245 /
           SRS30216) GN=Krad_0249 PE=3 SV=1
          Length = 431

 Score = 34.3 bits (77), Expect = 6.2
 Identities = 30/113 (26%), Positives = 50/113 (44%)
 Frame = +3

Query: 192 QQSRSFMYLAGRDAADRKAETKAYMRLGVRGIRREPISQAQRNAELEEEAKRIDLAPHKL 371
           +Q R+     G+  A  K E K  +  GV+ +     +   R+ E  EE + +  +   +
Sbjct: 53  EQLRAEQKSRGKLVAAAKGEEKQQLLAGVKELSERVKAAEARSNEAGEEFEALLRSIPNV 112

Query: 372 LEKGEPGTMQAIIKDVMPVGYFLTLPTGREGYLPALDLGFTGGIVILERLFKV 530
           +E G P   +     +  VG   +LP G + +   LDLG   G + +ER  KV
Sbjct: 113 VEDGVPAGGEDDYVVLEEVGSPPSLPEGLDAWRDHLDLGERLGAIDMERGAKV 165


>tr|Q4K792|Q4K792_PSEF5 Transcriptional regulator, AraC family
           OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
           GN=PFL_4810 PE=4 SV=1
          Length = 309

 Score = 33.9 bits (76), Expect = 8.0
 Identities = 29/126 (23%), Positives = 53/126 (42%), Gaps = 4/126 (3%)
 Frame = +3

Query: 180 LGRSQQSRSFMYLAGRDAADRKAETKAYMRLGVRGIRREPISQAQRNAELEEEAKRIDLA 359
           + R   S  F  +  + A    A+ +  +RL       +P++    +  +    K   ++
Sbjct: 45  VSRHSSSHQFGSVLAQPALCIMAQGRKEVRLADESYNYDPLNYLVVSVSVPLSGKVAIVS 104

Query: 360 PHK----LLEKGEPGTMQAIIKDVMPVGYFLTLPTGREGYLPALDLGFTGGIVILERLFK 527
           P +    L    +PG + A+I D  P+G     PTGR  Y+  LD      ++ L RL  
Sbjct: 105 PEEPILALRLDIDPGEINALIADAGPIG-VPARPTGRGLYVERLDPAMLDAVLRLTRLLD 163

Query: 528 VGQELT 545
             +++T
Sbjct: 164 TPKDIT 169