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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK958194
Clone id TST39A01NGRL0030_F20
Library
Length 296
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0030_F20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
GTGTGCTTGTATAAGAGGAGGTGGTCGCCTCTTTCTCTCTCCATCTAAGTCTGCCTCTTT
GTCTCCATTTGAATGCGTGAGAGCATCTTGAGTGTCACTGTTGATGGCTTGCAACCGGGA
CAATTGTTGTTCGTGTGCTGGCAAAGCGGAAGAATTGTGCATCAGCAATGGTGATTCTCT
CGGAGCTGAGAGCATGGACTTCGAAGGGGGTGAGCTCCATTGAAGGTTTTCTTTTATTTG
ATGCTTTGTAAACCTATTCTGGCGAAGTATTCGAGCAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id Q8CIN9
Definition sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus norvegicus
Align length 65
Score (bit) 31.6
E-value 1.3
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK958194|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0030_F20, 5'
         (276 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Ratt...    32   1.3  
sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=n...    31   1.7  
sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus...    31   2.2  
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no...    30   2.9  
sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage...    30   2.9  
sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680 OS=A...    30   3.8  
sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase OS...    30   3.8  
sp|Q5XPI3|RN123_MOUSE E3 ubiquitin-protein ligase RNF123 OS=Mus ...    29   6.4  
sp|O15117|FYB_HUMAN FYN-binding protein OS=Homo sapiens GN=FYB P...    29   6.4  
sp|Q91081|ADRA2_LABOS Alpha-2 adrenergic receptor OS=Labrus ossi...    29   6.4  
sp|P40556|YIA6_YEAST Uncharacterized mitochondrial carrier YIL00...    29   8.3  
sp|Q7ZXT3|EDC4_XENLA Enhancer of mRNA-decapping protein 4 OS=Xen...    29   8.4  

>sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus
           norvegicus GN=Rffl PE=1 SV=1
          Length = 362

 Score = 31.6 bits (70), Expect = 1.3
 Identities = 19/65 (29%), Positives = 33/65 (50%)
 Frame = -1

Query: 210 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 31
           P  ++ L+ P+ S +    S  LP+   Q++ + A + +TQ A+ H + D E  +  E  
Sbjct: 156 PEQQAFLTQPQSSTV-PPTSPGLPSSPAQVTSVLAQDQETQQAIGHVSQDHEEPIFLEST 214

Query: 30  RRPPP 16
            R PP
Sbjct: 215 ARVPP 219


>sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=ninE
           PE=3 SV=1
          Length = 56

 Score = 31.2 bits (69), Expect = 1.7
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -3

Query: 253 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTR 131
           +YK  N+RK   EL P E+  L     ++ D ++ R A+ R
Sbjct: 13  IYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARR 53


>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
           musculus GN=Rasa2 PE=1 SV=1
          Length = 848

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -2

Query: 143 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 48
           CQ T+ + PGCKP T  +    +I ++  R+T
Sbjct: 727 CQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
           norvegicus GN=Rasa2 PE=2 SV=1
          Length = 847

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -2

Query: 143 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 48
           CQ T+   PGCKP T  +    +I ++  R+T
Sbjct: 726 CQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage
           DLP12 OS=Escherichia coli (strain K12) GN=ninE PE=3 SV=1
          Length = 56

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = -3

Query: 253 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTRT 128
           +Y+  N+RK   EL P E+  L     ++ D ++ R A+ R+
Sbjct: 13  IYRVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARRS 54


>sp|Q93Z92|RING4_ARATH E3 ubiquitin-protein ligase At4g11680
           OS=Arabidopsis thaliana GN=At4g11680 PE=2 SV=1
          Length = 390

 Score = 30.0 bits (66), Expect = 3.8
 Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
 Frame = -2

Query: 188 QLRE---NHHC*CTILPLCQHTNNNCPGCK 108
           +LRE   NHH  CT +    H N+ CP CK
Sbjct: 350 ELRELPCNHHFHCTCIDKWLHINSRCPLCK 379


>sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase
           OS=Desulfotalea psychrophila GN=pnp PE=3 SV=1
          Length = 692

 Score = 30.0 bits (66), Expect = 3.8
 Identities = 21/62 (33%), Positives = 30/62 (48%)
 Frame = +2

Query: 20  GGRLFLSPSKSASLSPFECVRAS*VSLLMACNRDNCCSCAGKAEELCISNGDSLGAESMD 199
           GGR+     K      F  + +S + L++AC R+      GKA+EL  S  + LGA    
Sbjct: 150 GGRIAYIDGKYVLNPTFAELESSSMDLMVACTRNAIVMVEGKADEL--SEDEILGAIFFA 207

Query: 200 FE 205
           FE
Sbjct: 208 FE 209


>sp|Q5XPI3|RN123_MOUSE E3 ubiquitin-protein ligase RNF123 OS=Mus
           musculus GN=Rnf123 PE=2 SV=1
          Length = 1314

 Score = 29.3 bits (64), Expect = 6.4
 Identities = 17/57 (29%), Positives = 27/57 (47%)
 Frame = -1

Query: 213 SPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLD 43
           SPP +   +A    PL  HN   LP H  QL ++ + ++++Q  +    G     LD
Sbjct: 44  SPPDRGPAAATSRKPLNFHN---LPEHVDQLLQVDSEDNESQGQVEGRLGPSTVVLD 97


>sp|O15117|FYB_HUMAN FYN-binding protein OS=Homo sapiens GN=FYB PE=1
           SV=1
          Length = 783

 Score = 29.3 bits (64), Expect = 6.4
 Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 4/27 (14%)
 Frame = -1

Query: 105 INSDTQDALTHSNG----DKEADLDGE 37
           +N D QD +THS+G    D+E D +GE
Sbjct: 434 VNEDNQDGVTHSDGAGNLDEEQDSEGE 460


>sp|Q91081|ADRA2_LABOS Alpha-2 adrenergic receptor OS=Labrus
           ossifagus PE=3 SV=1
          Length = 432

 Score = 29.3 bits (64), Expect = 6.4
 Identities = 13/28 (46%), Positives = 15/28 (53%)
 Frame = -2

Query: 230 KTFNGAHPLRSPCSQLRENHHC*CTILP 147
           +T NG +   SPC   REN HC C   P
Sbjct: 237 QTENGLNKANSPCHGDRENGHCQCPPTP 264
tr_hit_id Q4E5A5
Definition tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypanosoma cruzi
Align length 66
Score (bit) 36.6
E-value 0.65
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK958194|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0030_F20, 5'
         (276 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypa...    37   0.65 
tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein OS=Trypa...    35   1.5  
tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory trans...    35   2.5  
tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative unchara...    34   3.2  
tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subs...    34   3.2  
tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein OS=Talar...    34   3.2  
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t...    34   3.2  
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish...    34   4.2  
tr|Q10DW0|Q10DW0_ORYSJ Os03g0717900 protein OS=Oryza sativa subs...    33   5.5  
tr|A3AM41|A3AM41_ORYSJ Putative uncharacterized protein OS=Oryza...    33   5.5  
tr|A2XLF0|A2XLF0_ORYSI Putative uncharacterized protein OS=Oryza...    33   5.5  
tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein OS=Trich...    33   5.5  
tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (st...    33   5.5  
tr|A9RSV2|A9RSV2_PHYPA Predicted protein OS=Physcomitrella paten...    33   7.2  
tr|B6KMK8|B6KMK8_TOXGO Putative uncharacterized protein OS=Toxop...    33   7.2  
tr|Q570Z6|Q570Z6_MOUSE MKIAA4127 protein (Fragment) OS=Mus muscu...    33   9.4  
tr|B2KF50|B2KF50_MOUSE UHRF1 (ICBP90) binding protein 1 OS=Mus m...    33   9.4  
tr|B0KS50|B0KS50_PSEPG Amino acid adenylation domain protein OS=...    33   9.4  
tr|B6Q3E0|B6Q3E0_PENMA Putative uncharacterized protein OS=Penic...    33   9.4  
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe...    33   9.4  
tr|A9HKD8|A9HKD8_9RHOB Dehydrogenase, putative OS=Roseobacter li...    33   9.4  

>tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein
            OS=Trypanosoma cruzi GN=Tc00.1047053506559.250 PE=4 SV=1
          Length = 1559

 Score = 36.6 bits (83), Expect = 0.65
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = -1

Query: 210  PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINS-DTQDALTHSNGDKEADLDGER 34
            PPS S+ S PR SP L  +SS+ PA ++    LQAI++ D+ D      GD  +    E 
Sbjct: 1179 PPSSSLASPPRSSPSLFSSSSS-PATDKHRRVLQAISTVDSSDVAALEKGDDSSPQAQEE 1237

Query: 33   KRRPPP 16
                 P
Sbjct: 1238 TAEQEP 1243


>tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein
            OS=Trypanosoma brucei GN=Tb11.01.8390 PE=4 SV=1
          Length = 2135

 Score = 35.4 bits (80), Expect = 1.5
 Identities = 19/53 (35%), Positives = 29/53 (54%)
 Frame = -1

Query: 195  MLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGE 37
            +LS P  SP L+ N   LPA     SRL  ++    +A + ++GD E + +GE
Sbjct: 1038 ILSNPASSPQLLLNMCKLPAPAYMGSRLPLLSMPVHNARSDTSGDVEGETNGE 1090


>tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory
           transducer OS=Rhodopseudomonas palustris (strain BisA53)
           GN=RPE_1941 PE=3 SV=1
          Length = 568

 Score = 34.7 bits (78), Expect = 2.5
 Identities = 15/33 (45%), Positives = 23/33 (69%)
 Frame = -1

Query: 210 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRL 112
           PP + ++ A  E+ L +HN S LPAH Q+L++L
Sbjct: 63  PPPEYVIEAFLEATLALHNPSNLPAHRQRLAKL 95


>tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative
           uncharacterized protein) OS=Oryza sativa subsp. japonica
           GN=OJ1200_C08.114 PE=4 SV=1
          Length = 860

 Score = 34.3 bits (77), Expect = 3.2
 Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
 Frame = -1

Query: 240 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 76
           Q++ ++ +SSP   S L     S   + P  + N S  P H  + +RL  + +  +    
Sbjct: 73  QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 132

Query: 75  HSNGDKEADLDGERKRRPPP 16
           + +G++EA + GER+++  P
Sbjct: 133 YIDGEQEATILGERQKQNSP 152


>tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subsp.
           japonica GN=Os07g0686400 PE=4 SV=1
          Length = 926

 Score = 34.3 bits (77), Expect = 3.2
 Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
 Frame = -1

Query: 240 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 76
           Q++ ++ +SSP   S L     S   + P  + N S  P H  + +RL  + +  +    
Sbjct: 139 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 198

Query: 75  HSNGDKEADLDGERKRRPPP 16
           + +G++EA + GER+++  P
Sbjct: 199 YIDGEQEATILGERQKQNSP 218


>tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein
           OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_027460 PE=4
           SV=1
          Length = 266

 Score = 34.3 bits (77), Expect = 3.2
 Identities = 23/70 (32%), Positives = 36/70 (51%)
 Frame = -1

Query: 246 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSN 67
           K +++E+L   SPP+ S L++P  + ++ H      AHE  LSR     +  +D     N
Sbjct: 27  KEKMEESLD--SPPTGSPLTSPIVTIVVGHERRLFAAHEDVLSRSAFFEAALKDQFLEPN 84

Query: 66  GDKEADLDGE 37
           G K+ DL  E
Sbjct: 85  G-KKVDLPDE 93


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
           transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
           PE=2 SV=3
          Length = 398

 Score = 34.3 bits (77), Expect = 3.2
 Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
 Frame = +2

Query: 2   CACIRGGGR-LFLSPSKSASLSPFECVRAS*VSLLMACNRDN----CCS-CAGKAEELCI 163
           C C  G  R +   PS  A   P+ CV     S L +CNR N    C S C+   E  C 
Sbjct: 65  CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 164 SNGDS 178
             G S
Sbjct: 125 KQGTS 129


>tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein
           OS=Leishmania braziliensis GN=LbrM20_V2.4220 PE=4 SV=1
          Length = 1189

 Score = 33.9 bits (76), Expect = 4.2
 Identities = 19/57 (33%), Positives = 32/57 (56%)
 Frame = -1

Query: 192 LSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERKRRP 22
           L  P   PL+   SSA P  E  ++   A+++++  A T + G+ ++   GE+KRRP
Sbjct: 428 LHHPTLPPLVKAESSAAPTAESPVANRGALSAESA-AATANTGESDSSPAGEKKRRP 483


>tr|Q10DW0|Q10DW0_ORYSJ Os03g0717900 protein OS=Oryza sativa subsp.
           japonica GN=Os03g0717900 PE=2 SV=1
          Length = 455

 Score = 33.5 bits (75), Expect = 5.5
 Identities = 24/66 (36%), Positives = 30/66 (45%), Gaps = 8/66 (12%)
 Frame = -1

Query: 210 PPSKSMLSAPRESPLLMHNSSALPAH--------EQQLSRLQAINSDTQDALTHSNGDKE 55
           PPS S+     ESP+    S  L  H        EQQL +LQ      +DA   + GDK 
Sbjct: 118 PPSSSIAPLQMESPVGQFLSQILATHPHLLPAAAEQQLEQLQT----DRDAAKDNGGDKP 173

Query: 54  ADLDGE 37
           A  DG+
Sbjct: 174 APSDGD 179


>tr|A3AM41|A3AM41_ORYSJ Putative uncharacterized protein OS=Oryza
           sativa subsp. japonica GN=OsJ_011863 PE=4 SV=1
          Length = 413

 Score = 33.5 bits (75), Expect = 5.5
 Identities = 24/66 (36%), Positives = 30/66 (45%), Gaps = 8/66 (12%)
 Frame = -1

Query: 210 PPSKSMLSAPRESPLLMHNSSALPAH--------EQQLSRLQAINSDTQDALTHSNGDKE 55
           PPS S+     ESP+    S  L  H        EQQL +LQ      +DA   + GDK 
Sbjct: 76  PPSSSIAPLQMESPVGQFLSQILATHPHLLPAAAEQQLEQLQT----DRDAAKDNGGDKP 131

Query: 54  ADLDGE 37
           A  DG+
Sbjct: 132 APSDGD 137