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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK959295
Clone id TST39A01NGRL0004_E20
Library
Length 659
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_E20. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
GTCAGACTCTGCATCGCGAGGATGAAGGCGGGCGCCGTTCTACAAAGCAAGGCGCCATGA
ATGCTCGGGGCTTGTTGCGTAGTTCAGGACGAGCCCTGTAGCCCTACTGTGCAAGGCATC
GAATCATCGATTCGTTTTTTGCTGTGCATCCCGAGGATGAAGGCGGCCTTGATTAACGAT
GATGCAGAAGGGCTGCGATGTCTGCTTGCTGGGGATGCTCTGTGCGCATTGAATGCCTCA
TGGGAGAGTCCTTTTCAACGCGTACAGCTTTTTGCGCCTACTGTGTTGCTCACAACCTAC
TGAGAACTTGCCAATTGGTGCTGCGAACAGTAGTGTGTGCTTGTATAAGAGGAGGTGGTC
GCCTCTTTCTCTCTCCATCTAAGTCTGCCTCTTTGTCTCCATTTGAATGCGTGAGAGCAT
CTTGAGTGTCACTGTTGATGGCTTGCAACCGGGACAATTGTTGTTCGTGTGCTGGCAAAG
CGGAAGAATTGTGCATCAGCAATGGTGATTCTCTCGGAGCTGAGAGCATGGACTTCGAAG
GGGGTGAGCTCCATTGAAGGTTTTCTTTTATTTGATGCTTTGTAAACCTATTCTGGCGAA
GTATTCGAGCATCTCTGTGTCGAAATTGCGGATCTGATGTGCTTCATTCCTAGCAAGAA
■■Homology search results ■■ -
sp_hit_id Q8CIN9
Definition sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus norvegicus
Align length 65
Score (bit) 31.6
E-value 3.9
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959295|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0004_E20, 5'
         (659 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Ratt...    32   3.9  
sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=n...    31   5.1  
sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Maca...    27   5.3  
sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus...    31   6.6  
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no...    30   8.6  
sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage...    30   8.6  

>sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus
           norvegicus GN=Rffl PE=1 SV=1
          Length = 362

 Score = 31.6 bits (70), Expect = 3.9
 Identities = 19/65 (29%), Positives = 33/65 (50%)
 Frame = -2

Query: 544 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 365
           P  ++ L+ P+ S +    S  LP+   Q++ + A + +TQ A+ H + D E  +  E  
Sbjct: 156 PEQQAFLTQPQSSTV-PPTSPGLPSSPAQVTSVLAQDQETQQAIGHVSQDHEEPIFLEST 214

Query: 364 RRPPP 350
            R PP
Sbjct: 215 ARVPP 219


>sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=ninE
           PE=3 SV=1
          Length = 56

 Score = 31.2 bits (69), Expect = 5.1
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -1

Query: 587 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTR 465
           +YK  N+RK   EL P E+  L     ++ D ++ R A+ R
Sbjct: 13  IYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARR 53


>sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Macaca
           fascicularis GN=ZNF541 PE=2 SV=2
          Length = 804

 Score = 27.3 bits (59), Expect(2) = 5.3
 Identities = 19/70 (27%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
 Frame = -2

Query: 550 SSPPSKSMLSAPRESPLLM-HNSSALPA--HEQQLSRLQAINSDTQDALTHSNGDKEADL 380
           S P +K  L +   +PL++ H+   +P   H   ++  Q  + + +D+       KE+  
Sbjct: 343 SPPGAKKPLDSTAAAPLVVPHSIPVVPVTRHVGSMAMEQEKDGEERDS-------KESSQ 395

Query: 379 DGERKRRPPP 350
             +RK+RPPP
Sbjct: 396 QRKRKKRPPP 405



 Score = 20.8 bits (42), Expect(2) = 5.3
 Identities = 10/34 (29%), Positives = 14/34 (41%)
 Frame = -3

Query: 297 GCEQHSRRKKLYALKRTLP*GIQCAQSIPSKQTS 196
           GC Q   R  ++ + R L    QC+   P    S
Sbjct: 424 GCHQSRLRSPMFLVDRLLKGLFQCSPYTPPPMLS 457


>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
           musculus GN=Rasa2 PE=1 SV=1
          Length = 848

 Score = 30.8 bits (68), Expect = 6.6
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -3

Query: 477 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 382
           CQ T+ + PGCKP T  +    +I ++  R+T
Sbjct: 727 CQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
           norvegicus GN=Rasa2 PE=2 SV=1
          Length = 847

 Score = 30.4 bits (67), Expect = 8.6
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 477 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 382
           CQ T+   PGCKP T  +    +I ++  R+T
Sbjct: 726 CQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage
           DLP12 OS=Escherichia coli (strain K12) GN=ninE PE=3 SV=1
          Length = 56

 Score = 30.4 bits (67), Expect = 8.6
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = -1

Query: 587 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTRT 462
           +Y+  N+RK   EL P E+  L     ++ D ++ R A+ R+
Sbjct: 13  IYRVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARRS 54
tr_hit_id B0CPT8
Definition tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82)
Align length 120
Score (bit) 40.4
E-value 0.097
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959295|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0004_E20, 5'
         (659 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (st...    40   0.097
tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor (st...    38   0.63 
tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypa...    37   1.4  
tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein OS=Trich...    36   1.8  
tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein OS=Trypa...    35   3.1  
tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory trans...    35   5.3  
tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative unchara...    34   7.0  
tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subs...    34   7.0  
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t...    34   7.0  
tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein OS=Talar...    34   7.0  
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish...    34   9.1  
tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative OS=Pe...    34   9.1  
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe...    34   9.1  

>tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor
           (strain S238N-H82) GN=LACBIDRAFT_186956 PE=4 SV=1
          Length = 940

 Score = 40.4 bits (93), Expect = 0.097
 Identities = 29/120 (24%), Positives = 54/120 (45%), Gaps = 4/120 (3%)
 Frame = -2

Query: 580 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLS----RLQAINSDTQDAL 413
           +H +   LQ  +    ++ S   +  LL +    +P    ++S    RL   + DT+D  
Sbjct: 557 RHVLDSELQQRACEFFALASRSDDDELLQNICEEMPPFPPRVSALLGRLNRKHGDTEDKR 616

Query: 412 THSNGDKEADLDGERKRRPPPLIQAHTTVRSTNWQVLSRL*ATQ*AQKAVRVEKDSPMRH 233
           T  +G KEA+LD E  +R   L++ + T  S N  ++  L     +  +++    SP  +
Sbjct: 617 TWIHGGKEANLDREATKRKTFLVENNGTASSDNQGIMDSLAGLDMSSPSIQAIGSSPQEY 676


>tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor
           (strain S238N-H82) GN=LACBIDRAFT_322427 PE=4 SV=1
          Length = 194

 Score = 37.7 bits (86), Expect = 0.63
 Identities = 38/126 (30%), Positives = 50/126 (39%), Gaps = 4/126 (3%)
 Frame = -3

Query: 639 HQIRNFDTEMLEYFARIGLQSIK*KKTFNGAH---PLRSPCSQLRENHHC*C-TILPLCQ 472
           H++R  + E L  F        + K+  N AH   PLR   SQ    HH  C T +    
Sbjct: 79  HRLRRQEGEALFGFKA----KARDKQLVNNAHRRNPLRHKASQAPLPHHAMCETSMNRSD 134

Query: 471 HTNNNCPGCKPSTVTLKMLSRIQMETKRQT*MERERGDHLLLYKHTLLFAAPIGKFSVGC 292
               NC GC      L  L R    + RQT +  E G H+L  +     ++P G  S G 
Sbjct: 135 RRERNCCGC----ARLLGLPR----SPRQTLVNPEEGSHILWRREHYAGSSPFGLTSSGH 186

Query: 291 EQHSRR 274
             H  R
Sbjct: 187 RTHFTR 192


>tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein
            OS=Trypanosoma cruzi GN=Tc00.1047053506559.250 PE=4 SV=1
          Length = 1559

 Score = 36.6 bits (83), Expect = 1.4
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = -2

Query: 544  PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINS-DTQDALTHSNGDKEADLDGER 368
            PPS S+ S PR SP L  +SS+ PA ++    LQAI++ D+ D      GD  +    E 
Sbjct: 1179 PPSSSLASPPRSSPSLFSSSSS-PATDKHRRVLQAISTVDSSDVAALEKGDDSSPQAQEE 1237

Query: 367  KRRPPP 350
                 P
Sbjct: 1238 TAEQEP 1243


>tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein
           OS=Trichomonas vaginalis G3 GN=TVAG_067550 PE=4 SV=1
          Length = 398

 Score = 36.2 bits (82), Expect = 1.8
 Identities = 24/74 (32%), Positives = 37/74 (50%)
 Frame = -2

Query: 544 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 365
           P S S  S+PR+S    H SSA P++ + L      N D  D  + S+ DK     G  K
Sbjct: 74  PSSNSTPSSPRKSSA-KHRSSARPSNAKPLISDSKDNDDESD--SESSEDKSRPKTGGGK 130

Query: 364 RRPPPLIQAHTTVR 323
           R+P  +++  T ++
Sbjct: 131 RKPKKIVKKTTVIK 144


>tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein
            OS=Trypanosoma brucei GN=Tb11.01.8390 PE=4 SV=1
          Length = 2135

 Score = 35.4 bits (80), Expect = 3.1
 Identities = 19/53 (35%), Positives = 29/53 (54%)
 Frame = -2

Query: 529  MLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGE 371
            +LS P  SP L+ N   LPA     SRL  ++    +A + ++GD E + +GE
Sbjct: 1038 ILSNPASSPQLLLNMCKLPAPAYMGSRLPLLSMPVHNARSDTSGDVEGETNGE 1090


>tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory
           transducer OS=Rhodopseudomonas palustris (strain BisA53)
           GN=RPE_1941 PE=3 SV=1
          Length = 568

 Score = 34.7 bits (78), Expect = 5.3
 Identities = 15/33 (45%), Positives = 23/33 (69%)
 Frame = -2

Query: 544 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRL 446
           PP + ++ A  E+ L +HN S LPAH Q+L++L
Sbjct: 63  PPPEYVIEAFLEATLALHNPSNLPAHRQRLAKL 95


>tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative
           uncharacterized protein) OS=Oryza sativa subsp. japonica
           GN=OJ1200_C08.114 PE=4 SV=1
          Length = 860

 Score = 34.3 bits (77), Expect = 7.0
 Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
 Frame = -2

Query: 574 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 410
           Q++ ++ +SSP   S L     S   + P  + N S  P H  + +RL  + +  +    
Sbjct: 73  QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 132

Query: 409 HSNGDKEADLDGERKRRPPP 350
           + +G++EA + GER+++  P
Sbjct: 133 YIDGEQEATILGERQKQNSP 152


>tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subsp.
           japonica GN=Os07g0686400 PE=4 SV=1
          Length = 926

 Score = 34.3 bits (77), Expect = 7.0
 Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
 Frame = -2

Query: 574 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 410
           Q++ ++ +SSP   S L     S   + P  + N S  P H  + +RL  + +  +    
Sbjct: 139 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 198

Query: 409 HSNGDKEADLDGERKRRPPP 350
           + +G++EA + GER+++  P
Sbjct: 199 YIDGEQEATILGERQKQNSP 218


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
           transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
           PE=2 SV=3
          Length = 398

 Score = 34.3 bits (77), Expect = 7.0
 Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
 Frame = +3

Query: 336 CACIRGGGR-LFLSPSKSASLSPFECVRAS*VSLLMACNRDN----CCS-CAGKAEELCI 497
           C C  G  R +   PS  A   P+ CV     S L +CNR N    C S C+   E  C 
Sbjct: 65  CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 498 SNGDS 512
             G S
Sbjct: 125 KQGTS 129


>tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein
           OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_027460 PE=4
           SV=1
          Length = 266

 Score = 34.3 bits (77), Expect = 7.0
 Identities = 23/70 (32%), Positives = 36/70 (51%)
 Frame = -2

Query: 580 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSN 401
           K +++E+L   SPP+ S L++P  + ++ H      AHE  LSR     +  +D     N
Sbjct: 27  KEKMEESLD--SPPTGSPLTSPIVTIVVGHERRLFAAHEDVLSRSAFFEAALKDQFLEPN 84

Query: 400 GDKEADLDGE 371
           G K+ DL  E
Sbjct: 85  G-KKVDLPDE 93