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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK959462
Clone id TST39A01NGRL0004_M02
Library
Length 679
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_M02. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence 
CTCTCTCTTTCACACACACACACACACACACACACACCCACCTACACACCCAGAATGCTC
AAGCTCTAAGCCCATTTCCGGACCTAGGTTTGCACACAGGCGCTTGCTCTTCCCTGCTCC
CAGTTCACAGTCTCCTTAACCGGAGGAACATGCTTGGCAAAGGCCCATGCCTTCCTCGCA
CCAGCAGCAGCAGATCACTTCTCATTCCTTGTGAAGACTCAGATAACGACACAACTCTTT
GCAATAAGCCGCAGCAGGTAACGGGTCCTGCATTATGTTCTCCTTGCAAGATAATCATTG
GCTTCGAAGTGGAACCCGAGAAGAAGCCCCCCGTTGATGTGTGTGAGAGTCCTCGCTCTG
TCCTAGAGTCATCTGCTGTTCTGTCTTCCCATGATAAGCAGCAGGTAGTGGCAAGGTCGC
TCCGCTCTGGGTTAGGGAGCTTGCTTGCAGCCAAAAGCACTAGCGAGGAAGATGTTGGCA
CTGGCCTTGGCATTGTTGCTGAAGCTTCGGTTAAAAGCAAACATCATACTATTAGCAGCA
GCCCCTCCAAAGTGGTGACAGCTTGGCCTGCCAAAACTTATGATCATGATCCTATGAATT
ATGAGGTCTTTCCATTTCAAGAGCAAGGTCTGCATGTTAACTTGCAGGCGGATATGGAAG
ATCGGCTGTTGGATGCTGA
■■Homology search results ■■ -
sp_hit_id O35604
Definition sp|O35604|NPC1_MOUSE Niemann-Pick C1 protein OS=Mus musculus
Align length 82
Score (bit) 33.9
E-value 0.81
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959462|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0004_M02, 5'
         (679 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O35604|NPC1_MOUSE Niemann-Pick C1 protein OS=Mus musculus GN=...    34   0.81 
sp|Q21653|YNG2_CAEEL Uncharacterized protein R02F2.2 OS=Caenorha...    32   4.0  
sp|P70268|PKN1_MOUSE Serine/threonine-protein kinase N1 OS=Mus m...    31   6.9  
sp|P56941|NPC1_PIG Niemann-Pick C1 protein OS=Sus scrofa GN=NPC1...    31   6.9  
sp|Q8BHK1|NIPA1_MOUSE Non-imprinted in Prader-Willi/Angelman syn...    31   6.9  
sp|Q7RTP0|NIPA1_HUMAN Non-imprinted in Prader-Willi/Angelman syn...    31   6.9  

>sp|O35604|NPC1_MOUSE Niemann-Pick C1 protein OS=Mus musculus
           GN=Npc1 PE=2 SV=1
          Length = 1278

 Score = 33.9 bits (76), Expect = 0.81
 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
 Frame = +3

Query: 87  GLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKP-QQVT 263
           G    AC++   +  + N+ N  G+ P       S   ++  E   N T  CN+   +VT
Sbjct: 179 GRDARACNATNWIEYMFNKDN--GQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVT 236

Query: 264 GPALCSPCKIIIGFEVEPEKKP 329
           GP  C  C I+ G + +P   P
Sbjct: 237 GPCSCQDCSIVCGPKPQPPPPP 258


>sp|Q21653|YNG2_CAEEL Uncharacterized protein R02F2.2
            OS=Caenorhabditis elegans GN=R02F2.2/R02F2.3 PE=2 SV=4
          Length = 1182

 Score = 31.6 bits (70), Expect = 4.0
 Identities = 23/72 (31%), Positives = 33/72 (45%), Gaps = 12/72 (16%)
 Frame = +3

Query: 72   PFPDLGL----HTGACSSLLPVH--------SLLNRRNMLGKGPCLPRTSSSRSLLIPCE 215
            P PDL +    H+G C  LLPVH        S   +R+ L     +P   SS+ +L+ C 
Sbjct: 1101 PTPDLRVCEIGHSGPCRILLPVHTPSHSNHPSRKQKRSSLN----VPAQQSSQLMLVSCG 1156

Query: 216  DSDNDTTLCNKP 251
            +  +D T    P
Sbjct: 1157 EGLDDGTATQDP 1168


>sp|P70268|PKN1_MOUSE Serine/threonine-protein kinase N1 OS=Mus
           musculus GN=Pkn1 PE=1 SV=3
          Length = 946

 Score = 30.8 bits (68), Expect = 6.9
 Identities = 17/42 (40%), Positives = 22/42 (52%)
 Frame = +3

Query: 165 PCLPRTSSSRSLLIPCEDSDNDTTLCNKPQQVTGPALCSPCK 290
           P LP TS S S   P  +S     L ++ Q+  GP LCSP +
Sbjct: 571 PGLPSTSCSLSS--PTHESTTSPELPSETQETPGPGLCSPLR 610


>sp|P56941|NPC1_PIG Niemann-Pick C1 protein OS=Sus scrofa GN=NPC1
           PE=2 SV=1
          Length = 1277

 Score = 30.8 bits (68), Expect = 6.9
 Identities = 22/82 (26%), Positives = 35/82 (42%), Gaps = 1/82 (1%)
 Frame = +3

Query: 87  GLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKP-QQVT 263
           G    AC++   +  + N+ N  G+ P       S       E  +N T  C++   +VT
Sbjct: 178 GREAQACNATNWIEYMFNKDN--GQAPFTITPIFSDLPTHGMEPMNNATKGCDESVDEVT 235

Query: 264 GPALCSPCKIIIGFEVEPEKKP 329
           GP  C  C I+ G + +P   P
Sbjct: 236 GPCSCQDCSIVCGPKPQPPPPP 257


>sp|Q8BHK1|NIPA1_MOUSE Non-imprinted in Prader-Willi/Angelman
           syndrome region protein 1 homolog OS=Mus musculus
           GN=Nipa1 PE=2 SV=1
          Length = 323

 Score = 30.8 bits (68), Expect = 6.9
 Identities = 22/78 (28%), Positives = 38/78 (48%)
 Frame = +3

Query: 66  LSPFPDLGLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCN 245
           ++P   LG+  G   S+L  + L  + N+LGK  CL   + S  L+I    S++ TT   
Sbjct: 89  VTPLGALGVPFG---SILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTTQAE 145

Query: 246 KPQQVTGPALCSPCKIII 299
             +++T P       I++
Sbjct: 146 LEEKLTNPVFVGYLCIVL 163


>sp|Q7RTP0|NIPA1_HUMAN Non-imprinted in Prader-Willi/Angelman
           syndrome region protein 1 OS=Homo sapiens GN=NIPA1 PE=1
           SV=1
          Length = 329

 Score = 30.8 bits (68), Expect = 6.9
 Identities = 22/78 (28%), Positives = 38/78 (48%)
 Frame = +3

Query: 66  LSPFPDLGLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCN 245
           ++P   LG+  G   S+L  + L  + N+LGK  CL   + S  L+I    S++ TT   
Sbjct: 95  VTPLGALGVPFG---SILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTTQAE 151

Query: 246 KPQQVTGPALCSPCKIII 299
             +++T P       I++
Sbjct: 152 LEEKLTNPVFVGYLCIVL 169
tr_hit_id Q9JLG3
Definition tr|Q9JLG3|Q9JLG3_CRIGR Niemann-Pick type C1 protein OS=Cricetulus griseus
Align length 82
Score (bit) 35.8
E-value 2.6
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959462|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0004_M02, 5'
         (679 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q9JLG3|Q9JLG3_CRIGR Niemann-Pick type C1 protein OS=Cricetulu...    36   2.6  
tr|A8WTH2|A8WTH2_CAEBR CBR-MAX-2 protein OS=Caenorhabditis brigg...    35   5.7  
tr|Q7TMD4|Q7TMD4_MOUSE Niemann Pick type C1 OS=Mus musculus GN=N...    34   9.7  
tr|Q3U2B2|Q3U2B2_MOUSE Putative uncharacterized protein OS=Mus m...    34   9.7  

>tr|Q9JLG3|Q9JLG3_CRIGR Niemann-Pick type C1 protein OS=Cricetulus
           griseus GN=NPC1 PE=2 SV=1
          Length = 1277

 Score = 35.8 bits (81), Expect = 2.6
 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
 Frame = +3

Query: 87  GLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKP-QQVT 263
           G    AC++   +  + N+ N  G+ P       S   ++  E   N T  CN+   +VT
Sbjct: 178 GKDASACNATNWIEYMFNKNN--GQAPFTITPIFSDLPILGMEPMRNATKGCNESVDEVT 235

Query: 264 GPALCSPCKIIIGFEVEPEKKP 329
           GP  C  C I+ G + +P   P
Sbjct: 236 GPCSCQDCSIVCGPKPQPPPTP 257


>tr|A8WTH2|A8WTH2_CAEBR CBR-MAX-2 protein OS=Caenorhabditis briggsae
           GN=Cbr-max-2 PE=4 SV=1
          Length = 422

 Score = 34.7 bits (78), Expect = 5.7
 Identities = 48/195 (24%), Positives = 71/195 (36%), Gaps = 1/195 (0%)
 Frame = +3

Query: 96  TGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKPQQVTGPAL 275
           +  CSS L   S  +  N L  G  +  TSS   + +  +++ ND ++ +K    T  A 
Sbjct: 50  SSGCSSPLHHSSARSVGNSLSNGSVVSTTSSDGDVQLSNKENSNDKSVGDKNGNTTNKAA 109

Query: 276 CSPCKIIIGFEVEPEKKPPVDVCESPRSVLESSAVLSSHDKQQVVXXXXXXXXXXXXXXK 455
             P            ++PPV V  S R  L  + VL     +++V              K
Sbjct: 110 AEPA---------VSEEPPVRVRASHREKLSDTEVLD--QLREIV-------NPCNPLGK 151

Query: 456 STSEEDVGTGL-GIVAEASVKSKHHTISSSPSKVVTAWPAKTYDHDPMNYEVFPFQEQGL 632
              ++ +G G  G V  A      H   SS    V     KT     M        +Q  
Sbjct: 152 YEMKKQIGVGASGTVFVA------HVAGSSDVVAVKRMAFKTQPKKEMLLTEIKVMKQYR 205

Query: 633 HVNLQADMEDRLLDA 677
           H NL   +E  L+DA
Sbjct: 206 HPNLVNYIESYLVDA 220


>tr|Q7TMD4|Q7TMD4_MOUSE Niemann Pick type C1 OS=Mus musculus GN=Npc1
           PE=2 SV=1
          Length = 1277

 Score = 33.9 bits (76), Expect = 9.7
 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
 Frame = +3

Query: 87  GLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKP-QQVT 263
           G    AC++   +  + N+ N  G+ P       S   ++  E   N T  CN+   +VT
Sbjct: 178 GRDARACNATNWIEYMFNKDN--GQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVT 235

Query: 264 GPALCSPCKIIIGFEVEPEKKP 329
           GP  C  C I+ G + +P   P
Sbjct: 236 GPCSCQDCSIVCGPKPQPPPPP 257


>tr|Q3U2B2|Q3U2B2_MOUSE Putative uncharacterized protein OS=Mus
           musculus GN=Npc1 PE=2 SV=1
          Length = 1094

 Score = 33.9 bits (76), Expect = 9.7
 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
 Frame = +3

Query: 87  GLHTGACSSLLPVHSLLNRRNMLGKGPCLPRTSSSRSLLIPCEDSDNDTTLCNKP-QQVT 263
           G    AC++   +  + N+ N  G+ P       S   ++  E   N T  CN+   +VT
Sbjct: 178 GRDARACNATNWIEYMFNKDN--GQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVT 235

Query: 264 GPALCSPCKIIIGFEVEPEKKP 329
           GP  C  C I+ G + +P   P
Sbjct: 236 GPCSCQDCSIVCGPKPQPPPPP 257