DK959990
Clone id TST39A01NGRL0006_C24
Library
Length 656
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_C24. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID
Sequence
GAGCCCTGCTGTGAGGAGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACTCGGT
GTGTGCATGCATGTGTGTGTGAAAGCTTCCTCTGCGTTTCTGTGTGTGTGCGCGCAGCGC
ATTTGTGTATAGGAGAGAGCAAGGGCATCAGCCATGCCGTTCCGCAACGTTGCAGTGGGG
CGCTATGAGGAGTTCACCCACCCGGGGACGCTCAAATGTGGCCTAGCCGAATTCATCTCC
ACCCTCATCTTTGTCTTTGCAGGAGAAGGCTCTGTAATTGCCTATGCCAAATTGACCGAT
GGAGCGTCCTTGACACCCTCGGGACTCACTGGAGTGGCTATATGCCATGGGCTAGCCTTG
TTTGTGGCCGTGGCAATCTCGGCCAACATCTCGGGTGGGCATGTCAACCCTGCGGTCACT
TTCGGCCTGTTTGTCGGCGGTAACATCACCCTCTTCAAAGGCGTTATCTACTGGATTGCT
CAGCTGCTGGGAGCTGCAATTGCATGTCTCCTCCTCAAGCTTGTCACCGATGGCATGACC
ACCTCACCGCATGCCTTGTCAACTGGAGAGCCAGTATGGGGTGCCCTCGTCCTTGAGATC
GTGATCACATTTGGCCTTGTATACACAGTCTATGCCACAGCTGTTGATCCAAGATC
■■Homology search results ■■ -
sp_hit_id P50156
Definition sp|P50156|TIP11_ORYSJ Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica
Align length 167
Score (bit) 231.0
E-value 2.0e-60
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK959990|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_C24, 5'
(656 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|P50156|TIP11_ORYSJ Probable aquaporin TIP1-1 OS=Oryza sativa ... 231 2e-60
sp|O64964|TIP11_MAIZE Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=... 223 9e-58
sp|Q94CS9|TIP12_ORYSJ Probable aquaporin TIP1-2 OS=Oryza sativa ... 220 6e-57
sp|P25818|TIP11_ARATH Aquaporin TIP1-1 OS=Arabidopsis thaliana G... 217 5e-56
sp|Q9ATM0|TIP12_MAIZE Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=... 215 2e-55
sp|Q9FY14|TIP1_MEDTR Probable aquaporin TIP-type OS=Medicago tru... 208 2e-53
sp|O82598|TIP13_ARATH Aquaporin TIP1-3 OS=Arabidopsis thaliana G... 206 7e-53
sp|Q41963|TIP12_ARATH Aquaporin TIP1-2 OS=Arabidopsis thaliana G... 206 1e-52
sp|P42067|TIP1_MEDSA Probable aquaporin TIP-type OS=Medicago sat... 200 5e-51
sp|Q5Z6F0|TIP22_ORYSJ Probable aquaporin TIP2-2 OS=Oryza sativa ... 197 4e-50
sp|Q41951|TIP21_ARATH Aquaporin TIP2-1 OS=Arabidopsis thaliana G... 197 4e-50
sp|P24422|TIP2_TOBAC Probable aquaporin TIP-type RB7-18C OS=Nico... 190 5e-48
sp|P21653|TIP1_TOBAC Probable aquaporin TIP-type RB7-5A OS=Nicot... 189 1e-47
sp|Q84RL6|TIP23_MAIZE Aquaporin TIP2-3 OS=Zea mays GN=TIP2-3 PE=... 187 5e-47
sp|P33560|TIP_ANTMA Probable aquaporin TIP-type OS=Antirrhinum m... 186 7e-47
sp|Q7XA61|TIP21_ORYSJ Probable aquaporin TIP2-1 OS=Oryza sativa ... 185 2e-46
sp|Q9ATL8|TIP22_MAIZE Aquaporin TIP2-2 OS=Zea mays GN=TIP2-2 PE=... 184 4e-46
sp|Q9FGL2|TIP23_ARATH Aquaporin TIP2-3 OS=Arabidopsis thaliana G... 183 6e-46
sp|Q9ATL9|TIP21_MAIZE Aquaporin TIP2-1 OS=Zea mays GN=TIP2-1 PE=... 183 6e-46
sp|Q9FWV6|TIP31_ORYSJ Probable aquaporin TIP3-1 OS=Oryza sativa ... 182 1e-45
sp|Q41975|TIP22_ARATH Probable aquaporin TIP2-2 OS=Arabidopsis t... 182 2e-45
sp|Q9ATL7|TIP31_MAIZE Aquaporin TIP3-1 OS=Zea mays GN=TIP3-1 PE=... 181 2e-45
sp|P26587|TIP31_ARATH Aquaporin TIP3-1 OS=Arabidopsis thaliana G... 175 2e-43
sp|P23958|TIPA_PHAVU Probable aquaporin TIP-type alpha OS=Phaseo... 171 4e-42
sp|O22588|TIP32_ARATH Probable aquaporin TIP3-2 OS=Arabidopsis t... 169 9e-42
sp|Q9AT75|TIP32_MAIZE Aquaporin TIP3-2 OS=Zea mays GN=TIP3-2 PE=... 168 3e-41
sp|Q7XKI5|TIP32_ORYSJ Probable aquaporin TIP3-2 OS=Oryza sativa ... 145 1e-34
sp|Q9LWR2|TIP43_ORYSJ Probable aquaporin TIP4-3 OS=Oryza sativa ... 143 7e-34
sp|O82316|TIP41_ARATH Aquaporin TIP4-1 OS=Arabidopsis thaliana G... 141 3e-33
sp|Q9ATL3|TIP44_MAIZE Aquaporin TIP4-4 OS=Zea mays GN=TIP4-4 PE=... 139 1e-32

>sp|P50156|TIP11_ORYSJ Probable aquaporin TIP1-1 OS=Oryza sativa
subsp. japonica GN=TIP1-1 PE=2 SV=1
Length = 250

Score = 231 bits (590), Expect = 2e-60
Identities = 113/167 (67%), Positives = 134/167 (80%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG ++E HPG LK LAEFISTLIFVFAG+GS +A++KLT G + TP+GL
Sbjct: 1 MPIRNIAVGSHQEVYHPGALKAALAEFISTLIFVFAGQGSGMAFSKLTGGGATTPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
A+ H ALFVAV++ ANISGGHVNPAVTFG FVGGNITLF+G++YWIAQLLG+ +AC L
Sbjct: 61 AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWIAQLLGSTVACFL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
L+ T G+ T L TG VW ALVLEIV+TFGLVYTVYATAVDP+
Sbjct: 121 LRFSTGGLATGTFGL-TGVSVWEALVLEIVMTFGLVYTVYATAVDPK 166


>sp|O64964|TIP11_MAIZE Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=2
SV=1
Length = 250

Score = 223 bits (567), Expect = 9e-58
Identities = 108/167 (64%), Positives = 129/167 (77%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +A+G ++E HPG LK AEFISTLIFVFAG+GS +A++KLT G TP+GL
Sbjct: 1 MPINRIALGSHQEVYHPGALKAAFAEFISTLIFVFAGQGSGMAFSKLTGGGPTTPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
A+ H ALFVAV++ ANISGGHVNPAVTFG FVGGNITLF+G++YW+AQLLG+ +AC L
Sbjct: 61 AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWVAQLLGSTVACFL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
L+ T G T L TG VW ALVLEIV+TFGLVYTVYATAVDP+
Sbjct: 121 LRFSTGGQATGTFGL-TGVSVWEALVLEIVMTFGLVYTVYATAVDPK 166


>sp|Q94CS9|TIP12_ORYSJ Probable aquaporin TIP1-2 OS=Oryza sativa
subsp. japonica GN=TIP1-2 PE=2 SV=1
Length = 252

Score = 220 bits (560), Expect = 6e-57
Identities = 107/167 (64%), Positives = 126/167 (75%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +AVG E +HP T K +AEFIS LIFVFAG GS +A++KLTDG TPSGL
Sbjct: 1 MPVSRIAVGAPGELSHPDTAKAAVAEFISMLIFVFAGSGSGMAFSKLTDGGGTTPSGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
++ H LALFVAVA+ ANISGGHVNPAVTFG FVGGNI+L K V+YW+AQLLG+ +ACLL
Sbjct: 61 ASLAHALALFVAVAVGANISGGHVNPAVTFGAFVGGNISLVKAVVYWVAQLLGSVVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK+ T G +LS G W A+V EIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKIATGGAAVGAFSLSAGVGAWNAVVFEIVMTFGLVYTVYATAVDPK 167



Score = 33.5 bits (75), Expect = 1.0
Identities = 32/127 (25%), Positives = 49/127 (38%)
Frame = +1

Query: 172 AVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHG 351
AVG + G + E + T V+ YA D + +AI G
Sbjct: 129 AVGAFSLSAGVGAWNAVVFEIVMTFGLVYT------VYATAVDPKKGDLGVIAPIAI--G 180

Query: 352 LALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKLVTD 531
+ + G +NPAV+FG V + +YW+ +GAAIA L+ ++
Sbjct: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVTGVW-DNHWVYWLGPFVGAAIAALIYDIIFI 239

Query: 532 GMTTSPH 552
G PH
Sbjct: 240 GQ--RPH 244


>sp|P25818|TIP11_ARATH Aquaporin TIP1-1 OS=Arabidopsis thaliana
GN=TIP1-1 PE=1 SV=1
Length = 251

Score = 217 bits (552), Expect = 5e-56
Identities = 104/167 (62%), Positives = 125/167 (74%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+A+GR +E T P LK LAEFISTLIFV AG GS +A+ KLT+ + TPSGL
Sbjct: 1 MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
A+ H LFVAV++ ANISGGHVNPAVTFG F+GGNITL +G++YWIAQLLG+ +ACL+
Sbjct: 61 AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G+ LS G V A V EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPK 167



Score = 31.2 bits (69), Expect = 5.0
Identities = 28/106 (26%), Positives = 44/106 (41%)
Frame = +1

Query: 205 GTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHGLALFVAVAISA 384
G L + E + T V+ YA D + + + +AI G + +
Sbjct: 140 GVLNAFVFEIVMTFGLVYT------VYATAIDPKNGSLGTIAPIAI--GFIVGANILAGG 191

Query: 385 NISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKL 522
SG +NPAV FG V + T +YW L+G IA L+ ++
Sbjct: 192 AFSGASMNPAVAFGPAV-VSWTWTNHWVYWAGPLVGGGIAGLIYEV 236


>sp|Q9ATM0|TIP12_MAIZE Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2
SV=1
Length = 254

Score = 215 bits (547), Expect = 2e-55
Identities = 104/167 (62%), Positives = 128/167 (76%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +AVG E +HP T K +AEFISTLIFVFAG GS +A++KLTDG + TP+GL
Sbjct: 1 MPVSRIAVGAPGELSHPDTAKAAVAEFISTLIFVFAGSGSGMAFSKLTDGGAATPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
++ H LALFVAV++ ANISGGHVNPAVTFG FVGGNI+L K ++YW+AQLLG+ +ACLL
Sbjct: 61 ASLAHALALFVAVSVGANISGGHVNPAVTFGAFVGGNISLLKALVYWVAQLLGSVVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK+ T G +LS G A+VLE+V+TFGLVYTVYATAVDP+
Sbjct: 121 LKIATGGAALGAFSLSAGVGAMNAVVLEMVMTFGLVYTVYATAVDPK 167



Score = 35.0 bits (79), Expect = 0.35
Identities = 33/127 (25%), Positives = 50/127 (39%)
Frame = +1

Query: 172 AVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHG 351
A+G + G + + E + T V+ YA D + +AI G
Sbjct: 129 ALGAFSLSAGVGAMNAVVLEMVMTFGLVYT------VYATAVDPKKGDLGVIAPIAI--G 180

Query: 352 LALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKLVTD 531
+ + G +NPAV+FG V + V YW+ L GAAIA L+ ++
Sbjct: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVTGVWENHWV-YWVGPLAGAAIAALVYDIIFI 239

Query: 532 GMTTSPH 552
G PH
Sbjct: 240 GQ--RPH 244


>sp|Q9FY14|TIP1_MEDTR Probable aquaporin TIP-type OS=Medicago
truncatula GN=AQP1 PE=1 SV=1
Length = 250

Score = 208 bits (530), Expect = 2e-53
Identities = 110/167 (65%), Positives = 126/167 (75%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG +E THP TLK GLAEFIST IFVFAG GS IAY KLT+ + TP+GL
Sbjct: 1 MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
+I H ALFVAV++ ANISGGHVNPAVTFG FVGGNITL +G++Y IAQLLG+ +A L
Sbjct: 61 ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIVYIIAQLLGSIVASAL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
L VT + LS G V ALVLEIV+TFGLVYTVYATAVDP+
Sbjct: 121 LVFVT-ASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPK 166



Score = 32.0 bits (71), Expect = 2.9
Identities = 29/127 (22%), Positives = 55/127 (43%), Gaps = 26/127 (20%)
Frame = +1

Query: 223 LAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHGLA--------------- 357
L +++ + VF SV A+ L++G + P+ + + + GL
Sbjct: 112 LGSIVASALLVFVTASSVPAFG-LSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNI 170

Query: 358 -----LFVAVAISANI------SGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIA 504
+ + + ANI +G +NPAV+FG V + + +YW L+G IA
Sbjct: 171 GIIAPIAIGFIVGANILVGGAFTGASMNPAVSFGPAV-VSWSWSNHWVYWAGPLIGGGIA 229

Query: 505 CLLLKLV 525
L+ +++
Sbjct: 230 GLVYEVL 236


>sp|O82598|TIP13_ARATH Aquaporin TIP1-3 OS=Arabidopsis thaliana
GN=TIP1-3 PE=1 SV=1
Length = 252

Score = 206 bits (525), Expect = 7e-53
Identities = 98/167 (58%), Positives = 123/167 (73%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +A+G E + P ++ AEF S +IFVFAG+GS +AY KLT TP+GL
Sbjct: 1 MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
++ H ALFVAV++ AN+SGGHVNPAVTFG F+GGNITL + ++YWIAQLLGA +ACLL
Sbjct: 61 ASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK+ T GM T+ +LS G W A+V EIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGLVYTVYATAVDPK 167



Score = 33.5 bits (75), Expect = 1.0
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = +1

Query: 349 GLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKLVT 528
GL + + + G +NPAV+FG V I V YW+ +GAAIA ++ +
Sbjct: 180 GLIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHWV-YWVGPFIGAAIAAIVYDTIF 238

Query: 529 DG 534
G
Sbjct: 239 IG 240


>sp|Q41963|TIP12_ARATH Aquaporin TIP1-2 OS=Arabidopsis thaliana
GN=TIP1-2 PE=1 SV=2
Length = 253

Score = 206 bits (523), Expect = 1e-52
Identities = 104/168 (61%), Positives = 121/168 (72%), Gaps = 1/168 (0%)
Frame = +1

Query: 154 MPFRNVAVGRY-EEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLT 330
MP RN+A+G EE HP L+ LAEFISTLIFVFAG GS IA+ K+TD + TPSGL
Sbjct: 1 MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 331 GVAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACL 510
A+ H LFVAV++ ANISGGHVNPAVTFG+ +GGNITL +G++YWIAQLLG+ AC
Sbjct: 61 AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 511 LLKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LL T G LS G ALV EIV+TFGLVYTVYATAVDP+
Sbjct: 121 LLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPK 168



Score = 30.8 bits (68), Expect = 6.5
Identities = 27/107 (25%), Positives = 44/107 (41%)
Frame = +1

Query: 205 GTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHGLALFVAVAISA 384
G+L + E + T V+ YA D + + + +AI G + +
Sbjct: 141 GSLNALVFEIVMTFGLVYT------VYATAVDPKNGSLGTIAPIAI--GFIVGANILAGG 192

Query: 385 NISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKLV 525
SG +NPAV FG V + T +YW L+G +A ++ V
Sbjct: 193 AFSGASMNPAVAFGPAV-VSWTWTNHWVYWAGPLIGGGLAGIIYDFV 238


>sp|P42067|TIP1_MEDSA Probable aquaporin TIP-type OS=Medicago sativa
GN=MCP1 PE=2 SV=2
Length = 249

Score = 200 bits (509), Expect = 5e-51
Identities = 108/167 (64%), Positives = 124/167 (74%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG +E THP TLK GLAEFIST IFVFAG GS IAY KLT+ + TP+GL
Sbjct: 1 MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
+I H ALFVAV++ ANISGGHVNPAV FG FVGGNITL +G++Y IAQLLG+ + L
Sbjct: 61 ASIAHAFALFVAVSVGANISGGHVNPAV-FGAFVGGNITLLRGIVYIIAQLLGSIVCSAL 119

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
L VT + LS G V ALVLEIV+TFGLVYTVYATAVDP+
Sbjct: 120 LVFVT-ASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPK 165



Score = 31.2 bits (69), Expect = 5.0
Identities = 29/127 (22%), Positives = 54/127 (42%), Gaps = 26/127 (20%)
Frame = +1

Query: 223 LAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAICHGLA--------------- 357
L + + + VF SV A+ L++G + P+ + + + GL
Sbjct: 111 LGSIVCSALLVFVTASSVPAFG-LSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNI 169

Query: 358 -----LFVAVAISANI------SGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIA 504
+ + + ANI +G +NPAV+FG V + + +YW L+G IA
Sbjct: 170 GIIAPIAIGFIVGANILVGGAFTGASMNPAVSFGPAV-VSWSWSNHWVYWAGPLIGGGIA 228

Query: 505 CLLLKLV 525
L+ +++
Sbjct: 229 GLVYEVL 235


>sp|Q5Z6F0|TIP22_ORYSJ Probable aquaporin TIP2-2 OS=Oryza sativa
subsp. japonica GN=TIP2-2 PE=2 SV=1
Length = 248

Score = 197 bits (501), Expect = 4e-50
Identities = 100/163 (61%), Positives = 122/163 (74%)
Frame = +1

Query: 166 NVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTGVAIC 345
N+A GR+++ +LK +AEFISTL+FVFAG GS IAY KLT GA L P+GL VA+C
Sbjct: 4 NIAFGRFDDSFSAASLKAYVAEFISTLVFVFAGVGSAIAYTKLTGGAPLDPAGLVAVAVC 63

Query: 346 HGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLLLKLV 525
HG LFVAVAI ANISGGHVNPAVTFGL +GG IT+ GV YWIAQLLGA + +L++
Sbjct: 64 HGFGLFVAVAIGANISGGHVNPAVTFGLALGGQITILTGVFYWIAQLLGAIVGAVLVQFC 123

Query: 526 TDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
T G+ T H LS G + +V+EI++TFGLVYTVYATA DP+
Sbjct: 124 T-GVATPTHGLS-GVGAFEGVVMEIIVTFGLVYTVYATAADPK 164



Score = 34.3 bits (77), Expect = 0.59
Identities = 29/104 (27%), Positives = 48/104 (46%), Gaps = 2/104 (1%)
Frame = +1

Query: 220 GLAEFISTLIFVFAGEGSVIA-YAKLTDGASLTPSGLTGVAICHGLALFVAVAISANISG 396
G+ F ++ + G V YA D + + +AI G + + ++ SG
Sbjct: 135 GVGAFEGVVMEIIVTFGLVYTVYATAADPKKGSLGTIAPIAI--GFIVGANILVAGPFSG 192

Query: 397 GHVNPAVTFGLFV-GGNITLFKGVIYWIAQLLGAAIACLLLKLV 525
G +NPA +FG V G+ T IYW+ L+G +A L+ + V
Sbjct: 193 GSMNPARSFGPAVASGDYTNI--WIYWVGPLVGGGLAGLVYRYV 234


tr_hit_id A9PAL3
Definition tr|A9PAL3|A9PAL3_POPTR Putative uncharacterized protein OS=Populus trichocarpa
Align length 167
Score (bit) 248.0
E-value 2.0e-64
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK959990|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0006_C24, 5'
(656 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9PAL3|A9PAL3_POPTR Putative uncharacterized protein OS=Popul... 248 2e-64
tr|A9PJD6|A9PJD6_POPJC Putative uncharacterized protein OS=Popul... 242 1e-62
tr|A9P9I3|A9P9I3_POPTR Putative uncharacterized protein OS=Popul... 242 1e-62
tr|Q5DVT4|Q5DVT4_MIMPU Tonoplast intrinsic protein 1;1 OS=Mimosa... 240 5e-62
tr|Q6DW15|Q6DW15_9CARY Tonoplast intrinsic protein gamma OS=Sali... 239 1e-61
tr|Q9FXM0|Q9FXM0_PYRCO Gamma tonoplast intrinsic protein OS=Pyru... 236 9e-61
tr|A9NZ54|A9NZ54_PICSI Putative uncharacterized protein OS=Picea... 236 9e-61
tr|Q75T55|Q75T55_PRUPE Tonoplast intrinsic protein OS=Prunus per... 235 2e-60
tr|A7QJE3|A7QJE3_VITVI Chromosome chr8 scaffold_106, whole genom... 233 6e-60
tr|Q70AP5|Q70AP5_RICCO Aquaporin OS=Ricinus communis GN=Tip1-1 P... 231 2e-59
tr|A4ZYN4|A4ZYN4_ORYSJ cDNA clone:001-014-B06, full insert seque... 231 2e-59
tr|A2XCH7|A2XCH7_ORYSI Putative uncharacterized protein OS=Oryza... 231 2e-59
tr|A9NQU9|A9NQU9_PICSI Putative uncharacterized protein OS=Picea... 231 4e-59
tr|A5ARF0|A5ARF0_VITVI Putative uncharacterized protein OS=Vitis... 231 4e-59
tr|Q9XG82|Q9XG82_PICAB Major intrinsic protein OS=Picea abies GN... 230 7e-59
tr|O24048|O24048_MESCR Tonoplast intrinsic protein OS=Mesembryan... 229 9e-59
tr|Q0MX10|Q0MX10_VITVI Putative aquaporin OS=Vitis vinifera GN=T... 229 1e-58
tr|A7PQP6|A7PQP6_VITVI Chromosome chr6 scaffold_25, whole genome... 229 1e-58
tr|Q9SM37|Q9SM37_SPOST Putative gamma tonoplast intrinsic protei... 228 2e-58
tr|Q8H6N1|Q8H6N1_9ROSI Tonoplast intrinsic protein OS=Kandelia c... 228 3e-58
tr|O65860|O65860_PICAB Aquaporin-like protein OS=Picea abies GN=... 227 6e-58
tr|B2D2G7|B2D2G7_9CARY Tonoplast intrinsic protein OS=Tamarix hi... 226 7e-58
tr|A7PJ07|A7PJ07_VITVI Chromosome chr13 scaffold_17, whole genom... 225 2e-57
tr|Q5MBH8|Q5MBH8_VITVI Aquaporin OS=Vitis vinifera GN=TIP1;1 PE=... 225 2e-57
tr|A5B1B6|A5B1B6_VITVI Putative uncharacterized protein OS=Vitis... 225 2e-57
tr|Q307T0|Q307T0_PANGI Tonoplast intrinsic protein OS=Panax gins... 224 3e-57
tr|Q9LKL9|Q9LKL9_9MAGN Putative aquaporin TIP3 OS=Vitis berlandi... 224 5e-57
tr|B4FUH7|B4FUH7_MAIZE Aquaporin TIP1.1 OS=Zea mays PE=2 SV=1 223 1e-56
tr|A7J2I5|A7J2I5_WHEAT Tonoplast intrinsic protein OS=Triticum a... 222 1e-56
tr|Q8H1Z5|Q8H1Z5_BRAOB Tonoplast intrinsic protein bobTIP26-2 OS... 222 2e-56

>tr|A9PAL3|A9PAL3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 252

Score = 248 bits (634), Expect = 2e-64
Identities = 121/167 (72%), Positives = 138/167 (82%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG Y E T P LK LAEFISTLIFVFAGEGS +A++KLTDGAS TP+GL
Sbjct: 1 MPIRNIAVGHYRETTQPDALKAALAEFISTLIFVFAGEGSGMAFSKLTDGASNTPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI H ALFVAV++ ANISGGHVNPAVTFG F+GGNITLF+G++YWIAQLLG+ +ACLL
Sbjct: 61 AAIAHAFALFVAVSVGANISGGHVNPAVTFGAFIGGNITLFRGILYWIAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK VT G+ TS ALSTG VW A VLEIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKFVTGGLETSAFALSTGVGVWNAFVLEIVMTFGLVYTVYATAIDPK 167


>tr|A9PJD6|A9PJD6_POPJC Putative uncharacterized protein OS=Populus
jackii PE=2 SV=1
Length = 252

Score = 242 bits (618), Expect = 1e-62
Identities = 118/167 (70%), Positives = 136/167 (81%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG Y E T P L+ LAEFISTLIFVFAGEGS +A+AKLTDGA+ TP+GL
Sbjct: 1 MPIRNIAVGHYHEATQPDALRAALAEFISTLIFVFAGEGSGMAFAKLTDGAANTPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI H ALFVAV++ ANISGGHVNPAVTFG F+GGNITL +G++YWIAQLLG+ +ACLL
Sbjct: 61 AAIAHAFALFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G+ TS ALS+G VW A VLEIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKFTTGGLETSAFALSSGVGVWNAFVLEIVMTFGLVYTVYATAVDPK 167


>tr|A9P9I3|A9P9I3_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 252

Score = 242 bits (618), Expect = 1e-62
Identities = 118/167 (70%), Positives = 136/167 (81%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG Y E T P L+ LAEFISTLIFVFAGEGS +A+AKLTDGA+ TP+GL
Sbjct: 1 MPIRNIAVGHYHEATQPDALRAALAEFISTLIFVFAGEGSGMAFAKLTDGAANTPAGLIA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI H ALFVAV++ ANISGGHVNPAVTFG F+GGNITL +G++YWIAQLLG+ +ACLL
Sbjct: 61 AAIAHAFALFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G+ TS ALS+G VW A VLEIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKFTTGGLETSAFALSSGVGVWNAFVLEIVMTFGLVYTVYATAVDPK 167


>tr|Q5DVT4|Q5DVT4_MIMPU Tonoplast intrinsic protein 1;1 OS=Mimosa
pudica GN=tip1;1 PE=2 SV=1
Length = 251

Score = 240 bits (613), Expect = 5e-62
Identities = 116/167 (69%), Positives = 136/167 (81%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +N+A+GR EE THP TLK GLAEFISTLIFVFAG GS +A+ KLTD + TP+GL
Sbjct: 1 MPVQNIAIGRPEEATHPDTLKAGLAEFISTLIFVFAGSGSGMAFNKLTDNGATTPAGLIS 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI H ALFVAVA+ ANISGGHVNPAVTFG F+GGNI+L +G++YWIAQLLG+ +ACLL
Sbjct: 61 AAIAHAFALFVAVAVGANISGGHVNPAVTFGAFIGGNISLLRGIVYWIAQLLGSVVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK VT G+TT LS+G VW A+V EIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKFVTGGLTTPAFGLSSGVGVWNAVVFEIVMTFGLVYTVYATAVDPK 167


>tr|Q6DW15|Q6DW15_9CARY Tonoplast intrinsic protein gamma
OS=Salicornia herbacea PE=2 SV=2
Length = 254

Score = 239 bits (610), Expect = 1e-61
Identities = 116/167 (69%), Positives = 135/167 (80%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP +AVG E+ HP TLK GLAEFISTLIFV+AG+GS +A+AKLT GA+ TP+GL
Sbjct: 1 MPINRIAVGTTEDARHPNTLKAGLAEFISTLIFVYAGQGSGMAFAKLTAGAANTPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI HG ALFVAVA+ ANISGGHVNPAVTFG F+GGNITL G++YWIAQLLG+ +ACLL
Sbjct: 61 AAISHGFALFVAVAVGANISGGHVNPAVTFGAFIGGNITLLNGIVYWIAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G+ TS AL GE VWGA V+EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKFSTGGLQTSAFALCEGETVWGAFVMEIVMTFGLVYTVYATALDPK 167



Score = 34.3 bits (77), Expect = 6.9
Identities = 25/70 (35%), Positives = 34/70 (48%), Gaps = 7/70 (10%)
Frame = +1

Query: 358 LFVAVAISANI------SGGHVNPAVTFG-LFVGGNITLFKGVIYWIAQLLGAAIACLLL 516
L + + + ANI G +NPAV+FG V N IYW L+GA IA L+
Sbjct: 177 LAIGLIVGANILAGGAFDGASMNPAVSFGPALVSWNWA--NHWIYWAGPLIGAGIAGLIY 234

Query: 517 KLVTDGMTTS 546
+ + G T S
Sbjct: 235 EFIFMGSTDS 244


>tr|Q9FXM0|Q9FXM0_PYRCO Gamma tonoplast intrinsic protein OS=Pyrus
communis GN=Py-gTIP PE=2 SV=1
Length = 252

Score = 236 bits (602), Expect = 9e-61
Identities = 115/167 (68%), Positives = 136/167 (81%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVGR EE HP L+ LAEFISTLIFVFAGEGS +A+AKLTD A+ TP+GL
Sbjct: 1 MPIRNIAVGRPEETYHPDALRAALAEFISTLIFVFAGEGSGMAFAKLTDDAANTPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
A+ H LFVAV+ISANISGGHVNPAVTFG F+GGNI+L +G++YWIAQLLGA +AC L
Sbjct: 61 AALAHAFGLFVAVSISANISGGHVNPAVTFGAFIGGNISLLRGILYWIAQLLGATVACGL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK VT+G TT+ +LS+G VW A V EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKFVTNGQTTAAFSLSSGVGVWNAFVFEIVMTFGLVYTVYATAIDPK 167


>tr|A9NZ54|A9NZ54_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 253

Score = 236 bits (602), Expect = 9e-61
Identities = 117/167 (70%), Positives = 136/167 (81%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MPFR VA+GR EE THP LK LAE ISTLIFVFAGEGS +A+AKLT AS TP+GL
Sbjct: 1 MPFRGVAIGRPEEVTHPTALKAALAELISTLIFVFAGEGSGMAFAKLTSDASTTPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
VA+ HGL LFVAVA+ ANISGGHVNPAVTFG VGG+ITL +G++YWIAQL+GA +ACLL
Sbjct: 61 VALAHGLGLFVAVAVGANISGGHVNPAVTFGALVGGHITLLRGILYWIAQLIGATVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G++TS +LS+G V ALV EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKYTTGGLSTSAFSLSSGVGVGNALVFEIVMTFGLVYTVYATAIDPK 167


>tr|Q75T55|Q75T55_PRUPE Tonoplast intrinsic protein OS=Prunus
persica GN=Pr-gTIP1 PE=2 SV=1
Length = 252

Score = 235 bits (599), Expect = 2e-60
Identities = 115/167 (68%), Positives = 135/167 (80%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVGR EE HP L+ GLAEFISTLIFVFAGEGS +A+AKLT GA+ TP+GL
Sbjct: 1 MPIRNIAVGRPEEAYHPDALRAGLAEFISTLIFVFAGEGSGMAFAKLTHGAATTPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
A+ HG LFVAV++SANISGGHVNPAVTFG FVGGNI+L +G++YWIAQLLG+A+AC L
Sbjct: 61 AALAHGFGLFVAVSVSANISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSAVACGL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
L+ + TTS ALS G VW A V EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LRFAPNYQTTSAFALSEGVGVWNAFVFEIVMTFGLVYTVYATAIDPK 167


>tr|A7QJE3|A7QJE3_VITVI Chromosome chr8 scaffold_106, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00000605001
PE=3 SV=1
Length = 251

Score = 233 bits (595), Expect = 6e-60
Identities = 113/167 (67%), Positives = 132/167 (79%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+A GR EE T P LK AEFI TLIFVFAGEGS +A+ KLTD S TP+GL
Sbjct: 1 MPIRNIAFGRPEEATRPDALKAAFAEFICTLIFVFAGEGSGMAFNKLTDNGSTTPAGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
++ H ALFVAV++ ANISGGHVNPAVTFG FVGGNITL +G++YWIAQLLG+ +ACLL
Sbjct: 61 ASLAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGILYWIAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T+GMTTS +LS+G VW A V EIV+TFGLVYTVYATA+DP+
Sbjct: 121 LKFSTNGMTTSAFSLSSGVTVWNAFVFEIVMTFGLVYTVYATAIDPK 167


>tr|Q70AP5|Q70AP5_RICCO Aquaporin OS=Ricinus communis GN=Tip1-1 PE=2
SV=1
Length = 251

Score = 231 bits (590), Expect = 2e-59
Identities = 115/167 (68%), Positives = 130/167 (77%)
Frame = +1

Query: 154 MPFRNVAVGRYEEFTHPGTLKCGLAEFISTLIFVFAGEGSVIAYAKLTDGASLTPSGLTG 333
MP RN+AVG E T P LK LAEFISTLIFVFAGEGS +A+ KLT+ TPSGL
Sbjct: 1 MPIRNIAVGHPHEATQPDALKAALAEFISTLIFVFAGEGSGMAFNKLTNNGVATPSGLVA 60

Query: 334 VAICHGLALFVAVAISANISGGHVNPAVTFGLFVGGNITLFKGVIYWIAQLLGAAIACLL 513
AI H ALFVAV++ ANISGGHVNPAVTFG FVGGNITL +G++YW AQLLG+ +ACLL
Sbjct: 61 AAIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGILYWFAQLLGSTVACLL 120

Query: 514 LKLVTDGMTTSPHALSTGEPVWGALVLEIVITFGLVYTVYATAVDPR 654
LK T G+TTS ALS+G VW A V EIV+TFGLVYTVYATAVDP+
Sbjct: 121 LKFSTGGLTTSAFALSSGVGVWNAFVFEIVMTFGLVYTVYATAVDPK 167